Prioritization of sub-watersheds and subsequent site identification for soil water and conservation practices using the SWAT-AHP integrated model in the Lower Sutlej Sub-basin, India.

Prioritization of watersheds is a crucial step in integrated river basin management. It guides resource allocation, planning, decision-making, stakeholder engagement, targeted interventions, and monitoring and evaluation efforts. By identifying and addressing priority watersheds, comprehensive and sustainable management of water resources can be achieved within a river basin. The aim of the current study is to prioritize watersheds using the Soil and Water Assessment Tool (SWAT) and site suitability analysis for the implementation of soil and water conservation structures (SWCS) in the prioritized watersheds by using an analytical hierarchy process (AHP) of the Lower Sutlej Sub-basin, India. The model was calibrated with observed data from 2017 to 2019 with the first 2 years (2015-16) as a warm-up period. Furthermore, validation of the model was done using 2-year data from 2020 to 2021. The model showed successful performance in terms of the R2 range from 0.72 to 0.89, the NSE range from 0.67 to 0.73, the PBIAS range from - 26.70 to 11.30, and the RSR range from 0.51 to 0.57 for stream flow and sediment yield during calibration and validation period. The basin's average annual soil loss ranged from 3.08 to 21.63 t/ha/year, a watershed with the WS2 index seeing the highest rate of soil erosion (21.63 t/ha/year). Hence, WS2 was found to be a top priority. In addition, the site suitability analysis of the prioritized watershed (WS2) reveals that about 1.42% of the area is extremely suitable, 16.14% is highly suitable, and 35.58% is moderately suitable for SWCS. According to the site suitability map validation, both dam locations were found in highly suitable areas, so the developed site suitability map is accurate. This research will help sustainability planners and managers make more informed decisions when building SWCS at suitable sites for better land and water conservation.

Root plasticity: an effective selection technique for identification of drought tolerant maize (Zea mays L.) inbred lines.

The decline in tropical maize productivity due to climatic vulnerability is a matter of serious concern as being a food and feed/fodder commodity, it is an important crop for the sustenance of human life. Genetic selections and development of water deficit stress (WDS) tolerant commercial varieties have potential to offset the impact of changing temperatures and precipitation. For trait-specific genetic enhancement, there is a need to understand a suite of adaptation strategies for crop. We studied the response of various shoot and root traits in 71 maize inbreds of diverse origin under simulated sub-optimal water supply controlled conditions, delineated an array of traits which must be considered for selection for WDS and validated the inbreds harbouring tolerance to WDS for selection of authentic donor lines to develop WDS tolerant hybrids. A large data set was limited to uncorrelated traits based on principal component analysis and variability among maize lines was deciphered using heatmap dendrogram. We also reported the relevance of root anatomical plasticity to the inherent potential of lines to combat WDS. We recommend incorporating the changes in number and diameter of xylem and metaxylem under simulated controlled conditions as a part of precise phenotyping for WDS in maize. The study led to identification of WDS tolerant line LM22 in maize.

Geospatial technology for assessment of soil erosion and prioritization of watersheds using RUSLE model for lower Sutlej sub-basin of Punjab, India.

Erosion of soil by water coupled with human activities is considered as one of the most serious agents of land degradation, posing severe threat to agricultural productivity, soil health, water quality, and ecological setup. The assessment of soil erosion and recognition of problematic watersheds are pre-requisite for management of erosion hazards. In the present study, Revised Universal Soil Loss Equation (RUSLE) integrated with remote sensing (RS) and geographic information system (GIS) has been used to assess the soil erosion in lower Sutlej River basin of Punjab, India, and prioritize the watersheds for implementation of land and water conservation measures. The total basin area was about 8577 km2 which was divided into 14 sub-watersheds with the area ranging from 357.8 to 1354 km2. The data on rainfall (IMD gridded data), soil characteristics (FAO soil map), topography (ALOS PALSAR DEM) and land use (ESRI land use and land cover map) were prepared in the form of raster layers and overlaid together to determine the average annual soil loss. The results revealed that the average annual soil loss varied from 1.26 to 25 t ha-1, whereas total soil loss was estimated to be 2,441,639 tonnes. The spatial distribution map of soil erosion showed that about 94.4% and 4.7% of the total area suffered from very slight erosion (0-5 t ha-1 year-1) and slight erosion (5-10 t ha-1 year-1), respectively, whereas 0.11% (9.38 km2) experienced very severe soil loss (> 25 t ha-1 year-1). Based on estimated average annual soil loss of sub-watersheds, WS8 was assigned the highest priority for implementation of soil and water conservation measures (323.5 t ha-1 year-1), followed by WS9 (303.8 t ha-1 year-1), whereas WS2 was given last priority owing to its lowest value of soil loss (122.02 t ha-1 year-1). The present study urges that conservation strategies should be carried out in accordance with the priority ranking of diverse watersheds. These findings can certainly be used to implement soil conservation plans and management practices in order to diminish soil loss in the river basin.

Genome wide association studies for acid phosphatase activity at varying phosphorous levels in Brassica juncea L.

Acid phosphatases (Apases) are an important group of enzymes that hydrolyze soil and plant phosphoesters and anhydrides to release Pi (inorganic phosphate) for plant acquisition. Their activity is strongly correlated to the phosphorus use efficiency (PUE) of plants. Indian mustard (Brassica juncea L. Czern & Coss) is a major oilseed crop that also provides protein for the animal feed industry. It exhibits low PUE. Understanding the genetics of PUE and its component traits, especially Apase activity, will help to reduce Pi fertilizer application in the crop. In the present study, we evaluated 280 genotypes of the diversity fixed foundation set of Indian mustard for Apase activity in the root (RApase) and leaf (LApase) tissues at three- low (5µM), normal (250µM) and high (1mM) Pi levels in a hydroponic system. Substantial effects of genotype and Pi level were observed for Apase activity in both tissues of the evaluated lines. Low Pi stress induced higher mean RApase and LApase activities. However, mean LApase activity was relatively more than mean RApase at all three Pi levels. JM06016, IM70 and Kranti were identified as promising genotypes with higher LApase activity and increased R/S at low Pi. Genome-wide association study revealed 10 and 4 genomic regions associated with RApase and LApase, respectively. Annotation of genomic regions in the vicinity of peak associated SNPs allowed prediction of 15 candidates, including genes encoding different family members of the acid phosphatase such as PAP10 (purple acid phosphatase 10), PAP16, PNP (polynucleotide phosphorylase) and AT5G51260 (HAD superfamily gene, subfamily IIIB acid phosphatase) genes. Our studies provide an understanding of molecular mechanism of the Apase response of B. juncea at varying Pi levels. The identified SNPs and candidate genes will support marker-assisted breeding program for improving PUE in Indian mustard. This will redeem the crop with enhanced productivity under restricted Pi reserves and degrading agro-environments.

Synthesis and in vitro study of novel Mannich bases as antibacterial agents.

A series of novel Mannich bases derived from 5-chloro-2-methoxybenzamide and sulfonamides/amines have been synthesised and the antibacterial activities were evaluated against various Gram positive and Gram negative strains of bacteria. Some of the synthesized compounds showed superior in vitro activities as compared to their parent sulfonamides.