Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Brain Res ; 1130(1): 67-72, 2007 Jan 26.
Article in English | MEDLINE | ID: mdl-17174942

ABSTRACT

Wnt7a and HA-tagged Wnt7a have previously been shown to promote or delay neuronal differentiation respectively. In this study, we show that embryonic days 9.5 and 10.5 transgenic mouse embryos overexpressing Wnt7a specifically in nestin-positive neural stem/progenitor cells displayed a delay in neuronal differentiation, assayed by beta-tubulin III expression. Our results corroborate previous studies using HA-Wnt7a, and suggest a critical role for Wnt7a in control of neuronal progenitor maturation.


Subject(s)
Gene Expression Regulation, Developmental/physiology , Neurons/metabolism , Proto-Oncogene Proteins/metabolism , Stem Cells/metabolism , Tubulin/metabolism , Wnt Proteins/metabolism , Animals , Brain/cytology , Brain/embryology , Brain/metabolism , Cell Differentiation , Intermediate Filament Proteins/metabolism , Mice , Mice, Transgenic , Nerve Tissue Proteins/metabolism , Nestin , Neurons/cytology , Proto-Oncogene Proteins/genetics , Stem Cells/cytology , Wnt Proteins/genetics
2.
Mol Cell Neurosci ; 30(3): 437-51, 2005 Nov.
Article in English | MEDLINE | ID: mdl-16154760

ABSTRACT

Wnt7a has been reported to signal via the canonical pathway, but also in non-canonical pathways acting on the cytoskeleton. Since Wnt7a is expressed after neurulation, we set to investigate the effects of Wnt7a on brain regionalization. We engineered transgenic mouse embryos that, under control of the nestin second intron, overexpressed Wnt7a in neural stem/progenitor cells. Surprisingly, transgenic embryos failed to complete cranial neurulation due to reduced levels and an impaired distribution of actin microfilaments, beta-catenin, and N-cadherin at the neural tube adherens junctions. These transgenic embryos expressed high levels of Vangl2, an essential component of non-canonical Wnt signaling. In agreement with a disregulation of this pathway, aberrant spinal neurulation was detected in the transgenic embryos, revealing a novel function regulated by Wnts. Thus, our findings suggest that Wnt7a overexpression disrupts normal Wnt signaling in the neural tube, resulting in defective adherens junctions and neurulation.


Subject(s)
Adherens Junctions/metabolism , Central Nervous System/embryology , Central Nervous System/metabolism , Neurons/metabolism , Proto-Oncogene Proteins/metabolism , Stem Cells/metabolism , Wnt Proteins/metabolism , Actin Cytoskeleton/metabolism , Animals , Cadherins/metabolism , Cell Adhesion/genetics , Cell Communication/genetics , Cell Differentiation/genetics , Central Nervous System/cytology , Gene Expression Regulation, Developmental/genetics , Mice , Mice, Transgenic , Nerve Tissue Proteins/metabolism , Neural Tube Defects/genetics , Neural Tube Defects/metabolism , Neurons/cytology , Proto-Oncogene Proteins/genetics , Signal Transduction/physiology , Stem Cells/cytology , Up-Regulation/genetics , Wnt Proteins/genetics , beta Catenin/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL
...