Biological Properties of Yeast-based Mannoprotein for Prospective Biomedical Applications.

BACKGROUND: Natural products constitute more than half of all biomolecules lately being used in clinical settings. Mannoprotein derived from the yeast cell wall has found full biotechnological applications. OBJECTIVE: This study was intended to investigate the antioxidant, anticancer, and toxicological properties of Kluyveromyces marxianus mannoprotein (KM). METHODS: The KM extract was obtained through a sequence of operations, including centrifugation for cell isolation, precipitation with potassium citrate/sodium metabisulfite, and recovery and purification. Its antioxidant, growth inhibition, macrophage mitogenic, and toxic activities were evaluated for its future use in the biomedical field. RESULTS: Significant inhibitory effects of KM were obtained on reactive species. It showed antiproliferative activity against HeLa (human cervical adenocarcinoma) and MCF-7 (human breast cancer) cell lines with no toxic effects on HUVECs (human umbilical vein endothelial cells). The in vitro model of CHO-K1 (Chinese hamster ovary) cell lines did not show the cytotoxic and genotoxic of KM. Moreover, it enhanced macrophage activity in terms of nitric oxide (NO) production and viability. No sign of acute toxicity was found in BALB/c mice, and body weight remained unchanged in guinea pigs over three months. CONCLUSION: Comprehensive biological evaluations in this study are expected to expand the potential of KM as a natural material.

Lead and cadmium biosorption from milk by Lactobacillus acidophilus ATCC 4356.

The food and water contamination with heavy metals is increasing due to the environmental pollutions. Lead and cadmium are the toxic heavy metals for humans that can be found in air, soil, water, and even food. Lactic acid bacteria have the ability to remove and diminish the level of heavy metals. In this study, Lactobacillus acidophilus was used to remove lead and cadmium in milk and the capability of this valuable bacterium in biosorption of these metals low concentrations (µg/L or ppb) in milk was evaluated. First, the variables on lead and cadmium removal by this bacterium have been studied by Plackett-Burman design. Then, the bioremoval process was optimized and the three main factors, the bacterium concentration, contact time, and the initial heavy metal concentration were chosen by using a central composite design. The optimum lead and cadmium bioremoval yield of 80% and 75% were observed, respectively, at 1 × 1012 CFU of L. acidophilus in milk at the 4th day and the initial ion concentration of 100 µg/L. The 3D plots analysis showed the interaction effects on metal biosorption. This study showed that L. acidophilus is a natural effective biosorbent for lead and cadmium removal from milk.

An In Vitro and In Vivo Cholinesterase Inhibitory Activity of Pistacia khinjuk and Allium sativum Essential Oils.

OBJECTIVES: Alzheimer's disease (AD), an overwhelming neurodegenerative disease, has deleterious effects on the brain that consequently causes memory loss and language impairment. This study was intended to investigate the neuroprotective activity of the two essential oils (EOs) from Iranian Pistacia khinjuk (PK) leaves and Allium sativum (AS) cloves against ß-Amyloid 25-35 (Aß25-35) induced elevation of cholinesterase enzymes in AD. METHODS: The EOs of PK (PKEO) and AS (ASEO) were prepared and analyzed in terms of extraction yield, phenolic content, and cholinergic markers in vitro. Moreover, both were administered orally to adult male Wistar rats at concentrations of 1, 2, and 3%. The inhibitory potential of PKEO and ASEO was compared with Donepezil (0.75 mg/kg) against the high activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. RESULTS: PKEO reached an inhibition rate of 83.6% and 81.4% against AChE and BChE, respectively. ASEO had lower anti-cholinesterase activity (65.4% and 31.5% for the inhibition AChE and BChE). PKEO was found to have more phenolic content than ASEO. A significantly positive correlation was observed between the total phenolics and anti-cholinesterase potential. In rats, both EOs decreased the enzyme activity in a concentration-dependent manner. As compared with Donepezil, the significant difference in the AChE and BChE inhibition occurred as rats were treated with PKEO 3% (p < 0.05). CONCLUSION: It could be concluded that PKEO and ASEO are potent inhibitors of AChE and BChE in rats that hold promise to be used for the treatment of AD.

The interventional relationship between frequent fish consumption and depression symptoms in aging adults: a randomized controlled trial.

OBJECTIVE: The present investigation was intended to test the hypothesis that the elderly provided with the frequent consumption of fishes marinated in essential oil of Perilla frutescens (EOPF) or not would experience fewer depressive symptoms after 6 months. METHODS: A total of 180 participants were recruited from Sina Hospital, Mashhad, Iran, who were diagnosed with depression based on Diagnostic and Statistical Manual of Mental Disorders, 4th edition, Text Revision and Beck Depression Inventory. Participants (n = 180) were randomly assigned in a 1:1:1 ratio to Groups A, B, and C. The last two were provided with an instruction to consume Caspian white fish marinated in the presence or absence of EOPF (434 g each week or four meals per week). Group A served as the control with the common diet. The outcome measures were performed using the Beck Depression Inventory and the General Health Questionnaire. RESULTS: There were no statistically significant differences in depressive symptom scores between groups with frequent fish consumption as compared with the control (p > 0.05). Yet adjustment for covariates showed that there was a significant reduction in depression among them (p < 0.05). Moreover, consumption of fish and EOPF was associated with more considerable improvements than Groups A and B (p < 0.05). CONCLUSIONS: It could be concluded that high intakes of unsaturated fatty acids can afford to diminish likelihood of late-life depression. Copyright © 2017 John Wiley & Sons, Ltd.