The α2,3-selective potentiators of GABAA receptors, KRM-II-81 and MP-III-80, produce anxiolytic-like effects and block chemotherapy-induced hyperalgesia in mice without tolerance development.

Opiate analgesics are one of the treatment options for severe chronic pain, including late-stage cancer, chronic back pain and other disorders. The recent resurgence in opioid overdose has highlighted the serious need for alternative medicines for pain management. While a role for potentiators of α2/3-containing GABAA receptors in the modulation of pain has been known for several years, advancements in this area required data from selective compounds. KRM-II-81(5-(8-ethynyl-6-(pyridin-2-yl)-4H-benzo[f]imidazo[1,5-a][1,4]diazepin-3- yl)oxazole) and analogs selectively potentiate GABAA receptors containing α2/3 subunits and have recently been shown to attenuate pain behaviors in several acute and chronic pain models in rodents. The present study was designed to ascertain whether KRM-II-81 and the structural analog MP-III-80 (3-ethyl-5-(8-ethynyl-6-(pyridin-2-yl)-4H-benzo[f]imidazo[1,5-a][1,4]diazepin-3-yl)-1,2,4-oxadiazole) would block chemotherapeutic agent paclitaxel-induced pain in male, C57BL/6 mice. Both compounds significantly inhibited pain behaviors evoked by cold and tactile stimulation in paclitaxel-treated mice as did the neuropathic pain drug gabapentin. Subchronic dosing for 22 days with KRM-II-81 and MP-III-80 demonstrated enduring analgesic efficacy without tolerance development, while the effects of gabapentin showed evidence of tolerance development. KRM-II-81 and MP-III-80 also decreased marble-burying behavior in this mouse strain as did the anxiolytic drug chlordiazepoxide. In contrast to KRM-II-81 and MP-III-80, chlordiazepoxide had motor-impairing effects at anxiolytic-like doses. The data add to the literature documenting that these selective potentiators of α2/3-containing GABAA receptors are effective in a host of animal models used to detect novel analgesic drugs. The anxiolytic-like efficacy of these compounds fits well with the comorbidity of anxiety in patients with chronic pain and cancer.

A comparative evaluation between cheiloscopic patterns and the permanent molar relationships to predict the future malocclusions.

BACKGROUND: To assess the correlation between different cheiloscopic patterns with the permanent molar relationships. MATERIAL AND METHODS: 300 children who are 14-16 years old with completely erupted 2nd permanent molars upto occlusal table were recruited and the pattern of molar terminal plane was recorded in the proforma. Lip prints of these subjects were recorded with lipstick-cellophane method and middle 10mm of lower lip was analysed for the lip print pattern as suggested by Sivapathasundharam et al. The pattern were classified based on Tsuchihashi and Suzuki classification. RESULTS: Type II (branched) pattern was the most predominant cheiloscopic pattern. The predominant patterns which related to the Angle's classification were; type I (complete vertical) pattern for class I, type IV (reticular) pattern for class II and presence of type IV (reticular) pattern and absence of type I' (incomplete vertical) pattern for class III. In class III molar relationship, males showed an increased type II (branched) pattern and females showed an increased type IV (reticluar) pattern. CONCLUSIONS: Lip prints can provide an alternative to dermatoglyphics to predict malocclusions in permanent dentition. Further studies with larger sample size are required to provide an insight into its significant correlations. Key words:Cheiloscopy, Angle's classification, malocclusion.

Effect of protective coating on microleakage of conventional glass ionomer cement and resin-modified glass ionomer cement in primary molars: An In vitro study.

AIM: The aim of this in vitro study was to evaluate and compare the effect of protective coating G-Coat Plus on microleakage of conventional glass ionomer cement (CGIC) and resin-modified glass ionomer cement (RMGIC) in primary molars. MATERIALS AND METHODS: A total of 120 samples were divided into four groups with 30 samples in each group. Group A - teeth restored with CGIC. Group B - teeth restored with CGIC coated with G-Coat Plus. Group C - teeth restored with RMGIC. Group D - teeth restored with RMGIC coated with G-Coat Plus. Microleakage was tested using 2% basic fuschin dye penetration and measured at ×40 original magnification under the stereomicroscope. RESULTS: The results of this in vitro study showed that coating with G-Coat Plus showed a reduction in microleakage in CGIC and complete absence of microleakage in RMGIC when compared to the noncoated groups, which was statistically significant. CGIC without G-Coat Plus showed highest microleakage followed by RMGIC without G-Coat Plus, followed by a relatively less microleakage in CGIC with G-Coat Plus. CONCLUSION: Significant reduction in microleakage was seen in conventional GIC and RMGIC with G-Coat Plus when compared to conventional GIC and RMGIC without G-Coat Plus.

Preferences and choices of a child concerning the environment in a pediatric dental operatory.

BACKGROUND: The aim of this cross-sectional descriptive study was to determine children's preferences in a dental clinic so as to reduce anxiety during dental procedures. In this study survey methodology was used. MATERIALS AND METHODS: A questionnaire was designed to evaluate the child's preference in a dental hospital so as to remove anxiety during a dental procedure. This study was carried out on 50 children aged 6-10 years. The children were randomly selected based on their first dental visit in a private dental college. RESULTS: A large number of children preferred listening to rhymes and watching cartoons while undergoing dental treatment. They also preferred the walls painted with cartoons, the dental chair full of toys, a scented environment, and the presence of their parents during the treatment. CONCLUSION: The results of this study will help the dental team decide on the appropriate design of the pediatric dental operatory room in order to provide a comfortable dental environment which will reduce anxiety in children and improve the quality of health care.