ABSTRACT
This article displays raw data linked to the research article "Compositional Analysis and Biological Characterization of Cornus officinalis on Human 1.1B4 Pancreatic ß Cells" [1]. This data was generated by utilizing HPLC/(+and -)ESI-MSn on Cornus officinalis (CO) from four independent sources [1]. The aim was to identify the chemical profile of CO from multiple sources to compare the similarities and differences resulting from various processing methods, and compile a list of known and novel constituents to elucidate the bioactive ingredients. This report contains the full chromatogram and a raw list of the constituents found in CO including chemical name, retention time, and molecular weight from all four sources. All data from HPLC/MS analysis is raw and unprocessed.
ABSTRACT
Type 1 diabetes (T1D) is an autoimmune disease resulting from the loss of pancreatic ß cells and subsequent insulin production. Novel interventional therapies are urgently needed that can protect existing ß cells from cytokine-induced death and enhance their function before symptomatic onset. Our initial evidence is suggesting that bioactive ingredients within Cornus officinalis (CO) may be able to serve in this function. CO has been extensively used in Traditional Chinese Medicine (TCM) and reported to possess both anti-inflammatory and pro-metabolic effects. We hypothesize that CO treatment may provide a future potential candidate for interventional therapy for early stage T1D prior to significant ß cell loss. Our data demonstrated that CO can inhibit cytokine-mediated ß cell death, increase cell viability and oxidative capacity, and increase expression of NFATC2 (Nuclear Factor of Activated T Cells, Cytoplasmic 2). We have also profiled the bioactive components in CO from multiple sources by HPLC/MS (High Performance Liquid Chromatography/Mass Spectrometry) analysis. Altogether, CO significantly increases the energy metabolism of ß cells while inducing the NFAT pathway to signal for increased proliferation and endocrine function.
