ABSTRACT
The first Soviet kits for the serological identification of streptococci, groups A, B, and C, on the basis of the coagglutination test were developed. Each kit was intended for 35-40 determinations. The optimum concentration of streptococci during their identification by means of the reagents making up the kit was about 1.6 x 10(9) cells/ml. The specificity of the reagents in comparison with the results of the identification of streptococci by reference methods was 97.3 +/- 0.9%. The reagents making up the kits can be presumably used for solving a number of practical problems in the epidemiological surveillance of streptococcal infection.
Subject(s)
Serotyping/instrumentation , Streptococcus agalactiae/classification , Streptococcus pyogenes/classification , Streptococcus/classification , Agglutination Tests/instrumentation , Agglutination Tests/standards , Evaluation Studies as Topic , Humans , Latex Fixation Tests , Precipitin Tests , Reagent Kits, Diagnostic/standards , Reference Standards , Russia , Sensitivity and Specificity , Serotyping/standards , Streptococcal Infections/diagnosisABSTRACT
The influence of sodium nucleinate on the growth kinetics, streptokinase activity and virulence of streptococcal populations, groups A and C, was studied. As revealed in these studies, the kinetics of the growth of the populations of both strains in the exponential phase did not depend on the concentration of sodium nucleinate in the culture medium. Measurements made on hours 15, 20 and 24 of growth showed the presence of close, direct and statistically significant correlation between the content of biomass, as well as streptokinase activity and specific streptokinase activity, and the concentration of sodium nucleinate in the culture medium. On the basis of the calculation of the coefficients of determination, the main part (70-96%) of the total dispersion of each of the above-mentioned characteristics dispersion of each of the above-mentioned characteristics could be attributed to variations in the concentration of sodium nucleinate. Five passages of faintly virulent streptococcal strains, groups A and C, were not accompanied by a rise in their virulence.
