Coexpression of all constituents of the cholesterol hydroxylase/lyase system in Escherichia coli cells.

Using the pTrc99A/P450scc vector, a plasmid was constructed in which cDNAs for cytochrome P450scc, adrenodoxin reductase, and adrenodoxin are situated in a single expression cassette. This plasmid was shown to direct the synthesis of all the above proteins in Escherichia coli. Their localization in the E. coli cells and stoichiometry were determined. Cell homogenates exhibited cholesterol hydroxylase/lyase activity, due to catalytically active forms of all three proteins. Thus, the full set of constituents of the mammalian cholesterol hydroxylase/lyase system was shown to be synthesized in bacterial cells for the first time.

[The importance of x-ray and endoscopic studies of the stomach in the complex examination of patients following resections and vagotomies]. / Znachenie rentgenologicheskogo i éndoskopicheskogo issledovanii zheludka v kompleksnom obsledovanii bol'nykh posle rezektsii i vagotomii.

X-ray and endoscopic examinations were made in the course of follow-up of patients surgically treated for gastroduodenal ulcer (n = 134). X-ray investigations of postoperative stomach make it possible to assess it functionally, to determine its relations with other parts of gastrointestinal tract. Endoscopy is helpful in revealing changes in gastric mucosa, anastomosis capable of producing diverse motor and evacuatory disturbances of the stomach. The methods are recommended in postoperative follow-up as these can identify early abnormalities and give information for relevant correction. The correction made by the authors resulted in a decrease in temporary disability by 2-3 weeks and in unfavorable outcomes from 17.2 to 4.3%.