[Anti-stress effect of dalargin in immobilization stress in rats]. / Antistressornyi éffekt dalargina pri immobilizatsionnom stresse u krys.

The function of the isolated perfused rat hearts was studied in four groups of experiments. Group 1--included the hearts of intact animals ("absolute control"), group 2--the hearts of rats subjected to 24 hour immobilization in supine position against the background of triple intramuscular injections of placebo (control), group 3 included the hearts of rats, which during 24 hour immobilization stress were thrice injected a synthetic analogue of endogenous opioids Dalargin in a dose of 3 g/kg, and group 4 included the hearts of animals, which during immobilization were administered Dalargin in a dose of 10 g/kg of body mass. Ulcer index as indicator of stress injury of gastric mucosa was also determined. In the control group of experiments (group 2) 24 hour immobilization stress resulted in complete depression of cardiac performance as compared with group 1, and ulcer index approximated 1. In group 3 the indices of cardiac performance even exceeded those in group 1 (intact animals). As compared with group 2, ulcer index in group 3 decreased by 9 times. In application of Dalargin in a dose of 10 g/kg complete preservation of heart function indices and complete prevention of stress injury of gastric mucosa were also observed. Thus, Dalargin possesses cardioprotective and anti-ulcerogenic effect in immobilization stress in rats. Most probably, this phenomenon can be attributed to its ability to inhibit the activity of sympathoadrenal system, which gets enhanced during stress.

[A comparative study of the effectiveness of regulatory peptides in acute experimental pancreatitis]. / Sravnitel'naia otsenka éffektivnosti reguliatornykh peptidov pri ostrom éksperimental'nom pankreatite.

The effect of regulatory peptides on the functional activity of pancreatic cells and phagocytic cells of reticuloendothelial system were determined in intact dogs against the background of acute experimental pancreatitis. Assessment of the efficacy of regulatory peptides was made with the aid of scintigraphic studies in gamma chamber using for this purpose colloid 198Au and 75Se-methionine. It was established that introduction of regulatory peptides to dogs leads to inhibition of capture of labeled methionine by pancreatocytes, practically not influencing the elimination of colloid gold from circulation.

[Increasing myocardial tolerance of global ischemia and reperfusion injury using a synthetic peptide]. / Povyshenie s pomo'shchiu sinteticheskogo peptida tolerantnosti miokarda k "globa'lnoi ishemii" i reperfuzionnym povrezhdeniiam.

The synthetic tripeptide Tyr-Pro-Arg was tested for its cardioprotective effect when added to the cardioplegic solution or when used during reperfusion (R) after "global myocardial ischemia" (GMI). After the baseline functional parameters had been recorded, the isolated perfused hearts (IPH) from rats with the working left ventricle were subjected to 30-minute GMI at 37 degrees C. When the tripeptide was added to the cardioplegic solution (3.10(-9) M), the degree of the functional recovery of IPH was higher after GMI than when a "pure" cardioplegic solution was applied. The functional parameters of IPH were restored to a considerable extent (up to 70-100%) when the tripeptide was added to the perfusate in the same concentration during R following GMI of 30-minute duration at 37 degrees C. Without the tripeptide, the function of IPH was not restored. Thus, the synthetic analogue of the endogenous opioids, Tyr-Pro-Arg" enhances the tolerance of rat IPH myocardium to exposure to GMI and R.

[Antiulcer activity of dibunol in experimental stomach and duodenal ulcers]. / Protivoiazvennaia aktivnost' dibunola pri éksperimental'nykh iazvakh zheludka i dvenadtsatiperstnoi kishki.

The antiulcer effect of two medicinal forms (oil solution, liniment) of dibunol in rats with different models of the stomach and duodenum ulcers was studied. The therapeutic activity of dibunol was evaluated by determining the index of ulcer formation as well as during the morphological and histochemical studies. The two medicinal forms of dibunol possessed equal antiulcer activity by all studied criteria. One should note a characteristic property of dibunol--its ability to produce pronounced epithelialization of ulceroerosive and ulceronecrotic lesions of the mucosa without rough scarring.

[Compensation of exocrine pancreatic insufficiency during its partial, subtotal and total resection]. / Kompensatsiia ékzokrinnoi nedostatochnosti podzheludochnoi zhelezy pri chastichnoi, subtotal'noi i total'noi ee rezektsii.

Chronic experiments on dogs have shown that the damage of extra-secretory pancreatic function by duct ligation caused marked compensatory changes of stomach function. The increase in gastric juice secretion and gastric juice proteolytic activity was accompanied by the reduction in its acidity. In addition to quantitative changes, qualitative shifts were revealed (amylolytic activity in strongly acid pH-reaction), never observed in the gastric juice of intact animals. Partial pancreas resection (up to 75%) both in control and test animals 10-14 months after pancreatic duct ligation was not accompanied by significant changes in gastric juice secretion. Total pancreas resection in dogs with previous pancreatic duct ligation caused neither prompt animal death, as in the control, nor the inhibition of compensatory reactions of gastric juice secretion.

[Inhibitory effect of leu-enkephalin on gastric secretion in dogs]. / Ingibiruiushchii éffekt lei-énkefalina na zheludochnuiu sekretsiiu u sobak.

The action of lei-enkephaline (EK) on gastric secretion was studied in various models: in 4 dogs with gastric fistula according to Basov, in 4 dogs with denervated ventricles according to Heidenheim, and on an isolated mucous membrane of the frog stomach. EK effect on gastrin content in the dog blood was also studied to show its significant inhibitory action on hydrochloric acid secretion in the dog stomach. It was shown that H+ isolation, both basal and EK-stimulated, by an isolated mucous membrane of the frog stomach remains unchanged. Blood gastrin level in the dog tended to dropping down under the effect of EK.

Functional and morphological adaptation of the gastric glands to disturbances of external pancreatic secretion and experimental reflux pancreatitis.

The mucous membrane of the gastric fundus was investigated in dogs after interruption of the external pancreatic secretion and the formation of experimental reflux pancreatitis. A marked increase in the number of chief cells and a decrease in the number of parietal cells were found in the gastric fundal glands between 6 and 15-17 months after the operation. Meanwhile the RNA concentration in the chief cells was increased. These changes were constant throughout the period of investigation (until 20 months). The results are in full agreement changes in the secretory function of the stomach described previously under the same conditions and they can therefore be regarded as being compensatory or adaptive in character.