Subject(s)
Adaptation, Physiological , Liver/physiology , Lung/physiology , Adaptation, Physiological/drug effects , Animals , Environmental Exposure , Histocytochemistry , Liver/drug effects , Lung/drug effects , Male , Oxidation-Reduction , Propane/analogs & derivatives , Propane/toxicity , Rats , Time FactorsABSTRACT
Simultaneous effect of benz(a)pyrene and sulphurous gas was accompanied by distinct alteration in metabolism of sialoglycoproteins in liver tissue; at the time N-acetylneuraminic acid was redistributed in liver subcellular organelles (mitochondria, lysosomes and endoplasmic reticulum) as well as cytochromes P-450 and b5 were induced in liver microsomes. The alterations observed appear to play a definite role in manifestation of the unfavourable effect in combined action of the carcinogen and chemical contaminations of environments.
Subject(s)
Benzopyrenes/pharmacology , Liver/drug effects , Sialoglycoproteins/metabolism , Sulfur Dioxide/pharmacology , Animals , Benzo(a)pyrene , Liver/metabolism , Lysosomes/drug effects , Lysosomes/metabolism , Male , Microsomes, Liver/drug effects , Microsomes, Liver/metabolism , Mitochondria, Liver/drug effects , Mitochondria, Liver/metabolism , Protein Binding/drug effects , Rats , Time FactorsABSTRACT
The authors performed a comparative biochemical study of some enzymes of lysosomic origin (hyaluronidase, N-acetyl-beta-D-glucosaminidase, beta-glucosidase, beta-galatosidase and acid phosphatase), of the state of enzyme substrate system N-acetylneuraminic acid---aldolase of neuramic acid and of the activity of lactatedehydrogenase (soluble in cytosol and bound on mitochodria) in the liver, lungs and blood serum of rats at various regimens of the inhalation action of CCl4. On the basis of results obtained they determined the biological importance of the change of activity of enzymes differently localized in cells at the adaptation of an organisme to the noxious action of CCl4.
Subject(s)
Carbon Tetrachloride/pharmacology , Fructose-Bisphosphate Aldolase/metabolism , Hydrolases/metabolism , L-Lactate Dehydrogenase/metabolism , Liver/enzymology , Animals , Cytoplasm/enzymology , Liver/ultrastructure , Lung/enzymology , Lysosomes/enzymology , Male , RatsSubject(s)
Enzymes/metabolism , Metals/toxicity , Testicular Diseases/chemically induced , Animals , Male , Organ Specificity , RatsABSTRACT
The authors effected a comparative examination of morphologic and biochemical investigation of structural and enzymic reactions of the rat liver during different periods of permanent inhalation influence of carbon tetrachloride. Three stages of morphologico-biochemical parllels in the development of hepatotropic effect characterized by certain regularities in the enzymic desorganization of lysosyme, mitochondrias and cytasol by derangement of the exchange of biopolymers containing carbohydrate (of glycoproteids, glycosaminoglycans) and DNK-synthetizing function in liver in comparison with control animals were revealed. Mechanisms of the hepatotropic effect and hygienic importance of structural function changes of hepatocytes due to action of factors polluting the atmosphere are appreciated.
Subject(s)
Carbon Tetrachloride/pharmacology , Liver/drug effects , Air Pollutants/adverse effects , Animals , Liver/cytology , Liver/enzymology , Male , RatsABSTRACT
Activity of lysosomal enzymes and content of carbohydrate containing biopolymers (glycosaminoglycans, glycoproteins) were altered in various tissues (liver, kidney, brain, aorta) of experimental animals intoxicated with carbon disulfide. Possible role of the impairments observed, related to neurotropic and hepatotoxic effects, is discussed. Subsequent development of atherogenic and embryotoxic effects of carbon disulfide--one of contaminators of surroundings--are considered.
