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1.
J Med Chem ; 56(7): 2738-46, 2013 Apr 11.
Article in English | MEDLINE | ID: mdl-23342998

ABSTRACT

HIV-1 reverse transcriptase (RT) undergoes a series of conformational changes during viral replication and is a central target for antiretroviral therapy. The intrinsic flexibility of RT can provide novel allosteric sites for inhibition. Crystals of RT that diffract X-rays to better than 2 Å resolution facilitated the probing of RT for new druggable sites using fragment screening by X-ray crystallography. A total of 775 fragments were grouped into 143 cocktails, which were soaked into crystals of RT in complex with the non-nucleoside drug rilpivirine (TMC278). Seven new sites were discovered, including the Incoming Nucleotide Binding, Knuckles, NNRTI Adjacent, and 399 sites, located in the polymerase region of RT, and the 428, RNase H Primer Grip Adjacent, and 507 sites, located in the RNase H region. Three of these sites (Knuckles, NNRTI Adjacent, and Incoming Nucleotide Binding) are inhibitory and provide opportunities for discovery of new anti-AIDS drugs.


Subject(s)
HIV Reverse Transcriptase/metabolism , Allosteric Site , Base Sequence , Crystallography, X-Ray , DNA Primers , HIV Reverse Transcriptase/chemistry , Models, Molecular , Protein Conformation
2.
Wiley Interdiscip Rev RNA ; 2(2): 277-98, 2011.
Article in English | MEDLINE | ID: mdl-21957010

ABSTRACT

The 5' mRNA cap structure is essential for efficient gene expression from yeast to human. It plays a critical role in all aspects of the life cycle of an mRNA molecule. Capping occurs co-transcriptionally on the nascent pre-mRNA as it emerges from the RNA exit channel of RNA polymerase II. The cap structure protects mRNAs from degradation by exonucleases and promotes transcription, polyadenylation, splicing, and nuclear export of mRNA and U-rich, capped snRNAs. In addition, the cap structure is required for the optimal translation of the vast majority of cellular mRNAs, and it also plays a prominent role in the expression of eukaryotic, viral, and parasite mRNAs. Cap-binding proteins specifically bind to the cap structure and mediate its functions in the cell. Two major cellular cap-binding proteins have been described to date: eukaryotic translation initiation factor 4E (eIF4E) in the cytoplasm and nuclear cap binding complex (nCBC), a nuclear complex consisting of a cap-binding subunit cap-binding protein 20 (CBP 20) and an auxiliary protein cap-binding protein 80 (CBP 80). nCBC plays an important role in various aspects of nuclear mRNA metabolism such as pre-mRNA splicing and nuclear export, whereas eIF4E acts primarily as a facilitator of mRNA translation. In this review, we highlight recent findings on the role of the cap structure and cap-binding proteins in the regulation of gene expression. We also describe emerging regulatory pathways that control mRNA capping and cap-binding proteins in the cell.


Subject(s)
Gene Expression Regulation , RNA Cap-Binding Proteins/physiology , RNA Caps/physiology , Active Transport, Cell Nucleus/genetics , Active Transport, Cell Nucleus/physiology , Alternative Splicing/genetics , Alternative Splicing/physiology , Animals , Cell Nucleus/metabolism , Humans , Models, Biological , Models, Molecular , Nonsense Mediated mRNA Decay/genetics , Nonsense Mediated mRNA Decay/physiology , RNA Cap-Binding Proteins/chemistry , RNA Cap-Binding Proteins/genetics , RNA Cap-Binding Proteins/metabolism , RNA Caps/chemistry , RNA Caps/genetics , RNA Caps/metabolism , RNA Processing, Post-Transcriptional
3.
Proc Natl Acad Sci U S A ; 108(25): 10104-8, 2011 Jun 21.
Article in English | MEDLINE | ID: mdl-21636784

ABSTRACT

The enzyme guanylyltransferase (GTase) plays a central role in the three-step catalytic process of adding an (m7)GpppN cap cotranscriptionally to nascent mRNA (pre-mRNAs). The 5'-mRNA capping process is functionally and evolutionarily conserved from unicellular organisms to human. However, the GTases from viruses and yeast have low amino acid sequence identity (∼25%) with GTases from mammals that, in contrast, are highly conserved (∼98%). We have defined by limited proteolysis of human capping enzyme residues 229-567 as comprising the minimum enzymatically active human GTase (hGTase) domain and have determined the structure by X-ray crystallography. Seven related conformational states of hGTase exist in the crystal. The GTP-binding site is evolutionarily and structurally conserved. The positional variations of the oligonucleotide/oligosaccharide binding fold lid domain over the GTP-binding site provide snapshots of the opening and closing of the active site cleft through a swivel motion. The pattern of conserved surface residues in mammals, but not in yeast, supports the finding that the recognition of the capping apparatus by RNA polymerase II and associated transcription factors is highly conserved in mammals, and the mechanism may differ somewhat from that in yeast. The hGTase structure should help in the design of biochemical and molecular biology experiments to explore the proteinprotein and proteinRNA interactions that ensure regulated transcription of genes in humans and other mammals.


Subject(s)
Nucleotidyltransferases/chemistry , Animals , Binding Sites , Crystallography, X-Ray , DNA Mutational Analysis , Fungal Proteins/chemistry , Fungal Proteins/genetics , Humans , Molecular Sequence Data , Nucleotidyltransferases/genetics , Protein Structure, Tertiary
4.
J Biol Chem ; 283(42): 28010-9, 2008 Oct 17.
Article in English | MEDLINE | ID: mdl-18703506

ABSTRACT

BMP2 (bone morphogenetic protein 2) is a multifunctional member of the transforming growth factor-beta family of growth factors. Disruption of BMP2 signaling results in developmental defects, cancers, and other diseases. BMP2 mRNAs are alternatively polyadenylated, resulting in mRNAs with distinct 3'-untranslated regions. The longer mRNA contains additional putative binding sites for post-transcriptional regulatory factors, including micro-RNAs. We combined functional assays with computational analyses of emerging genome data to define site- and species-specific polyadenylation determinants. In all mouse and human cell lines tested, shorter mRNAs resulting from using the first polyadenylation signal (PA1) were more abundant than mRNAs from the second signal (PA2). However, the PA1/PA2 usage ratios were 2-3-fold higher in human than in mouse cells. Expression of human BMP2 constructs in mouse cells and mouse constructs in human cells showed that cis-regulatory elements direct species-specific 3' processing of BMP2 transcripts. A 72-nucleotide region downstream of PA2 in the mouse sequence contains two novel cis-acting elements previously hypothesized to regulate polyadenylation in a bioinformatics analysis. Mutations that humanized the mouse-specific elements lowered the affinity for cleavage stimulation factor CstF64 and significantly weakened the PA2 signal relative to the PA1 signal. Thus, we have experimentally defined for the first time cis-regulatory elements that control a species-specific difference in the 3'-end processing of BMP2 and potentially of other genes.


Subject(s)
Bone Morphogenetic Protein 2/genetics , 3' Untranslated Regions , Animals , Base Sequence , Cell Line, Tumor , Chickens , Expressed Sequence Tags , Humans , Mice , Models, Biological , Molecular Sequence Data , Polyadenylation , Protein Structure, Tertiary , Sequence Homology, Nucleic Acid , Transfection
5.
Mol Cell Biol ; 28(19): 5829-36, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18678651

ABSTRACT

Addition of a 5' cap to RNA polymerase II transcripts, the first step of pre-mRNA processing in eukaryotes from yeasts to mammals, is catalyzed by the sequential action of RNA triphosphatase, guanylyltransferase, and (guanine-N-7)methyltransferase. The effects of knockdown of these capping enzymes in mammalian cells were investigated using T7 RNA polymerase-synthesized small interfering RNA and also a lentivirus-based inducible, short hairpin RNA system. Decreasing either guanylyltransferase or methyltransferase resulted in caspase-3 activation and elevated terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) staining characteristic of apoptosis. Induction of apoptosis was independent of p53 tumor suppressor but dependent on BAK or BAX. In addition, levels of the BH3 family member Bim increased, while Mcl-1 and Bik levels remained unchanged during apoptosis. In contrast to capping enzyme knockdown, apoptosis induced by cycloheximide inhibition of protein synthesis required BAK but not BAX. Both Bim and Mcl-1 levels decreased in cycloheximide-induced apoptosis while Bik levels were unchanged, suggesting that apoptosis in siRNA-treated cells is not a direct consequence of loss of mRNA translation. siRNA-treated BAK(-/-) BAX(-/-) double-knockout mouse embryonic fibroblasts failed to activate capase-3 or increase TUNEL staining but instead exhibited autophagy, as demonstrated by proteolytic processing of microtubule-associated protein 1 light chain 3 (LC3) and translocation of transfected green fluorescent protein-LC3 from the nucleus to punctate cytoplasmic structures.


Subject(s)
Acid Anhydride Hydrolases/genetics , Apoptosis , Autophagy , Methyltransferases/genetics , Nucleotidyltransferases/genetics , RNA, Small Interfering/genetics , Acid Anhydride Hydrolases/metabolism , Animals , Cell Line, Tumor , Humans , Methyltransferases/metabolism , Mice , Nucleotidyltransferases/metabolism , RNA Interference , bcl-2 Homologous Antagonist-Killer Protein/genetics , bcl-2 Homologous Antagonist-Killer Protein/metabolism , bcl-2-Associated X Protein/genetics , bcl-2-Associated X Protein/metabolism
6.
Nucleic Acids Res ; 36(15): 5083-92, 2008 Sep.
Article in English | MEDLINE | ID: mdl-18676450

ABSTRACT

HIV-1 reverse transcriptase (RT) is a primary target for anti-AIDS drugs. Structures of HIV-1 RT, usually determined at approximately 2.5-3.0 A resolution, are important for understanding enzyme function and mechanisms of drug resistance in addition to being helpful in the design of RT inhibitors. Despite hundreds of attempts, it was not possible to obtain the structure of a complex of HIV-1 RT with TMC278, a nonnucleoside RT inhibitor (NNRTI) in advanced clinical trials. A systematic and iterative protein crystal engineering approach was developed to optimize RT for obtaining crystals in complexes with TMC278 and other NNRTIs that diffract X-rays to 1.8 A resolution. Another form of engineered RT was optimized to produce a high-resolution apo-RT crystal form, reported here at 1.85 A resolution, with a distinct RT conformation. Engineered RTs were mutagenized using a new, flexible and cost effective method called methylated overlap-extension ligation independent cloning. Our analysis suggests that reducing the solvent content, increasing lattice contacts, and stabilizing the internal low-energy conformations of RT are critical for the growth of crystals that diffract to high resolution. The new RTs enable rapid crystallization and yield high-resolution structures that are useful in designing/developing new anti-AIDS drugs.


Subject(s)
Crystallography, X-Ray , HIV Reverse Transcriptase/chemistry , Nitriles/chemistry , Protein Engineering/methods , Pyrimidines/chemistry , Reverse Transcriptase Inhibitors/chemistry , Cloning, Molecular , Drug Design , HIV Reverse Transcriptase/genetics , HIV Reverse Transcriptase/metabolism , Models, Molecular , Mutagenesis , Rilpivirine
7.
Proc Natl Acad Sci U S A ; 105(5): 1466-71, 2008 Feb 05.
Article in English | MEDLINE | ID: mdl-18230722

ABSTRACT

TMC278 is a diarylpyrimidine (DAPY) nonnucleoside reverse transcriptase inhibitor (NNRTI) that is highly effective in treating wild-type and drug-resistant HIV-1 infections in clinical trials at relatively low doses ( approximately 25-75 mg/day). We have determined the structure of wild-type HIV-1 RT complexed with TMC278 at 1.8 A resolution, using an RT crystal form engineered by systematic RT mutagenesis. This high-resolution structure reveals that the cyanovinyl group of TMC278 is positioned in a hydrophobic tunnel connecting the NNRTI-binding pocket to the nucleic acid-binding cleft. The crystal structures of TMC278 in complexes with the double mutant K103N/Y181C (2.1 A) and L100I/K103N HIV-1 RTs (2.9 A) demonstrated that TMC278 adapts to bind mutant RTs. In the K103N/Y181C RT/TMC278 structure, loss of the aromatic ring interaction caused by the Y181C mutation is counterbalanced by interactions between the cyanovinyl group of TMC278 and the aromatic side chain of Y183, which is facilitated by an approximately 1.5 A shift of the conserved Y(183)MDD motif. In the L100I/K103N RT/TMC278 structure, the binding mode of TMC278 is significantly altered so that the drug conforms to changes in the binding pocket primarily caused by the L100I mutation. The flexible binding pocket acts as a molecular "shrink wrap" that makes a shape complementary to the optimized TMC278 in wild-type and drug-resistant forms of HIV-1 RT. The crystal structures provide a better understanding of how the flexibility of an inhibitor can compensate for drug-resistance mutations.


Subject(s)
Anti-HIV Agents/chemistry , Drug Resistance, Viral/genetics , Enzyme Inhibitors/chemistry , HIV Reverse Transcriptase/chemistry , Nitriles/chemistry , Pyrimidines/chemistry , Reverse Transcriptase Inhibitors/chemistry , Amino Acid Motifs/genetics , Amino Acid Sequence , Crystallography, X-Ray , Drug Design , HIV Reverse Transcriptase/genetics , Humans , Molecular Sequence Data , Mutation , Protein Conformation , Protein Engineering , Rilpivirine
8.
Proc Natl Acad Sci U S A ; 104(45): 17620-5, 2007 Nov 06.
Article in English | MEDLINE | ID: mdl-17978174

ABSTRACT

Cap formation is the first step of pre-mRNA processing in eukaryotic cells. Immediately after transcription initiation, capping enzyme (CE) is recruited to RNA polymerase II (Pol II) by the phosphorylated carboxyl-terminal domain of the Pol II largest subunit (CTD), allowing cotranscriptional capping of the nascent pre-mRNA. Recent studies have indicated that CE affects transcription elongation and have suggested a checkpoint model in which cotranscriptional capping is a necessary step for the early phase of transcription. To investigate further the role of the CTD in linking transcription and processing, we generated a fusion protein of the mouse CTD with T7 RNA polymerase (CTD-T7 RNAP). Unexpectedly, in vitro transcription assays with CTD-T7 RNAP showed that CE promotes formation of DNA.RNA hybrids or R loops. Significantly, phosphorylation of the CTD was required for CE-dependent R-loop formation (RLF), consistent with a critical role for the CTD in CE recruitment to the transcription complex. The guanylyltransferase domain was necessary and sufficient for RLF, but catalytic activity was not required. In vitro assays with appropriate synthetic substrates indicate that CE can promote RLF independent of transcription. ASF/SF2, a splicing factor known to prevent RLF, and GTP, which affects CE conformation, antagonized CE-dependent RLF. Our findings suggest that CE can play a direct role in transcription by modulating displacement of nascent RNA during transcription.


Subject(s)
Acid Anhydride Hydrolases/metabolism , Multienzyme Complexes/metabolism , Nucleotidyltransferases/metabolism , Transcription, Genetic , Humans
9.
Mol Cell Biol ; 25(7): 2644-9, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15767670

ABSTRACT

A characteristic feature of gene expression in eukaryotes is the addition of a 5'-terminal 7-methylguanine cap (m7GpppN) to nascent pre-mRNAs in the nucleus catalyzed by capping enzyme and cap methyltransferase. Small interfering RNA (siRNA) knockdown of cap methyltransferase in HeLa cells resulted in apoptosis as measured by terminal deoxynucleotidyltransferase-mediated dUTP-tetramethylrhodamine nick end labeling assay, demonstrating the importance of mRNA 5'-end methylation for mammalian cell viability. Nuclear localization of cap methyltransferase is mediated by interaction with importin-alpha, which facilitates its transport and selective binding to transcripts containing 5'-terminal GpppN. The methyltransferase 96-144 region has been shown to be necessary for importin binding, and N-terminal fusion of this sequence to nonnuclear proteins proved sufficient for nuclear localization. The targeting sequence was narrowed to amino acids 120 to 129, including a required 126KRK. Although full-length methyltransferase (positions 1 to 476) contains the predicted nuclear localization signals 57RKRK, 80KKRK, 103KKRKR, and 194KKKR, mutagenesis studies confirmed functional motifs only at positions 80, 103, and the previously unrecognized 126KRK. All three motifs can act as alternative nu clear targeting signals. Expression of N-truncated cap methyltransferase (120 to 476) restored viability of methyltransferase siRNA knocked-down cells. However, an enzymatically active 144-476 truncation mutant missing the three nuclear localization signals was mostly cytoplasmic and ineffective in preventing siRNA-induced loss of viability.


Subject(s)
Cell Nucleus/metabolism , Methyltransferases/chemistry , Methyltransferases/metabolism , Nuclear Localization Signals/physiology , RNA Caps/metabolism , Active Transport, Cell Nucleus , Amino Acid Motifs , Amino Acid Sequence , Apoptosis/genetics , Cell Survival , HeLa Cells , Humans , Methyltransferases/genetics , Mutation/genetics , Nuclear Localization Signals/genetics , Protein Binding , RNA Polymerase II/metabolism , RNA, Small Interfering/genetics , RNA, Small Interfering/metabolism , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , alpha Karyopherins/metabolism
10.
Proc Natl Acad Sci U S A ; 101(20): 7572-7, 2004 May 18.
Article in English | MEDLINE | ID: mdl-15136722

ABSTRACT

Capping of the 5' ends of nascent RNA polymerase II transcripts is the first pre-mRNA processing event in all eukaryotic cells. Capping enzyme (CE) is recruited to transcription complexes soon after initiation by the phosphorylation of Ser-5 of the carboxyl-terminal domain of the largest subunit of RNA polymerase II. Here, we analyze the role of CE in promoter clearance and its functional interactions with different factors that are involved in promoter clearance. FCP1-mediated dephosphorylation of the carboxyl-terminal domain results in a drastic decrease in cotranscriptional capping efficiency but is reversed by the presence of DRB sensitivity-inducing factor (DSIF). These results suggest involvement of DSIF in CE recruitment. Importantly, CE relieves transcriptional repression by the negative elongation factor, indicating a critical role of CE in the elongation checkpoint control mechanism during promoter clearance. This functional interaction between CE and the negative elongation factor documents a dynamic role of CE in promoter clearance beyond its catalytic activities.


Subject(s)
Promoter Regions, Genetic , RNA Caps/biosynthesis , RNA Polymerase II/metabolism , Nuclear Proteins/metabolism , Phosphoprotein Phosphatases/metabolism , Transcription Factors , Transcription, Genetic , Transcriptional Elongation Factors
11.
Proc Natl Acad Sci U S A ; 100(22): 12666-71, 2003 Oct 28.
Article in English | MEDLINE | ID: mdl-14569024

ABSTRACT

The HIV type 1 (HIV-1) Tat protein stimulates transcription elongation by recruiting P-TEFb (CDK9/cyclin T1) to the transactivation response (TAR) RNA structure. Tat-induced CDK9 kinase has been shown to phosphorylate Ser-5 of RNA polymerase II (RNAP II) C-terminal domain (CTD). Results presented here demonstrate that Tat-induced Ser-5 phosphorylation of CTD by P-TEFb stimulates the guanylyltransferase activity of human capping enzyme and RNA cap formation. Sequential phosphorylation of CTD by Tat-induced P-TEFb enhances the stimulation of human capping enzyme guanylyltransferase activity and RNA cap formation by transcription factor IIH-mediated CTD phosphorylation. Using an immobilized template assay that permits isolation of transcription complexes, we show that Tat/TAR-dependent phosphorylation of RNAP II CTD stimulates cotranscriptional capping of HIV-1 mRNA. Upon transcriptional induction of latently infected cells, accumulation of capped transcripts occurs along with Ser-5-phosphorylated RNAP II in the promoter proximal region of the HIV-1 genome. Therefore, these observations suggest that Tat/TAR-dependent phosphorylation of RNAP II CTD is crucial not only in promoting transcription elongation but also in stimulating nascent viral RNA capping.


Subject(s)
Gene Products, tat/metabolism , HIV-1/genetics , RNA Caps/genetics , RNA Polymerase II/chemistry , RNA Polymerase II/metabolism , RNA, Messenger/genetics , RNA, Viral/genetics , Binding Sites , Humans , Phosphorylation , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/metabolism , Ribonucleotides/metabolism , Transcription Factors/chemistry , Transcription Factors/metabolism , Transcription, Genetic , tat Gene Products, Human Immunodeficiency Virus
12.
J Biol Chem ; 278(16): 14168-73, 2003 Apr 18.
Article in English | MEDLINE | ID: mdl-12576475

ABSTRACT

Capping of the initiated 5' ends of RNA polymerase II products is evolutionarily and functionally conserved from yeasts to humans. The m(7)GpppN cap promotes RNA stability, processing, transport, and translation. Deletion of capping enzymes in yeasts was shown to be lethal due to rapid exonucleolytic degradation of uncapped transcripts or failure of capped but unmethylated RNA to initiate protein synthesis. Using RNA interference and Caenorhabditis elegans we have found that RNA capping is also essential for metazoan viability. C. elegans bifunctional capping enzyme was cloned, and capping activity by the expressed protein as well as growth complementation of yeast deletion strains missing either RNA triphosphatase or guanylyltransferase required terminal sequences not present in the previously isolated cel-1 clone. By RNA interference analysis we show that cel-1 is required for embryogenesis. cel-1(RNAi) embryos formed cytoplasmic granules characteristic of a phenocluster of RNA processing genes and died early in development.


Subject(s)
Nucleotidyltransferases/genetics , Nucleotidyltransferases/physiology , Acid Anhydride Hydrolases/metabolism , Amino Acid Sequence , Animals , Caenorhabditis elegans , Cytoplasm/metabolism , DNA, Complementary/metabolism , Drosophila , Gene Deletion , Genetic Complementation Test , Glutathione Transferase/metabolism , Humans , Molecular Sequence Data , Nucleotidyltransferases/chemistry , Nucleotidyltransferases/metabolism , Phenotype , Plasmids/metabolism , RNA Interference , Recombinant Fusion Proteins/metabolism , Saccharomyces cerevisiae/metabolism , Sequence Homology, Amino Acid
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