ABSTRACT
AA is the precursor of oxygenated metabolites called the eicosanoids, that are generated by the cyclooxygenase, lipoxygenase, or cytochrome P450 enzymatic pathways. The biologically active eicosanoids are labile, usually but not always, act near their site of synthesis, and are not stored in any tissue to any appreciable degree. In most tissues, there is a continuous low-level synthesis that is probably essential to normal function. Injury or surgical trauma can evoke a dramatic change in the quality and quantity of the metabolites. Pharmacologic manipulation of the pathways of AA metabolism, or alteration of the effects of AA metabolites, can significantly improve tissue perfusion in a variety of clinical situations. (See Figure 1 for a schematic summary of the pathways involved in arachidonic acid metabolites.
Subject(s)
Arachidonic Acids , Leukotrienes/physiology , Prostaglandins/physiology , Surgery, Plastic , Thromboxanes/physiology , Animals , Arachidonic Acid , Burns/physiopathology , Diet , Humans , Microcirculation , Surgical FlapsABSTRACT
To examine the contribution of arachidonic acid (AA) metabolites to the maintenance of cutaneous vasomotor tone after thermal injury, enzyme inhibitors were topically applied to the hamster cheek pouch before and after a spot burn. By use of video microscopy, blood flow was measured in adjacent arterioles that supplied the injured site. Ringer's solutions containing no drug (vehicle), indomethacin (cyclooxygenase inhibitor), BW755c (cyclooxygenase/lipoxygenase inhibitor), or ketoconazole (lipoxygenase/cytochrome P450 inhibitor) continuously suffused the entire tissue. There were no effects of these drugs on preburn blood flow at concentrations that blocked the vascular effects evoked by topical AA. In all groups, blood flow transiently increased after burn and thereafter decreased to levels that were altered by treatment. These results could not be attributed to alterations in vascular reactivity because neither the burn nor the drugs altered the vasodilation evoked by adenosine or prostacyclin. Relative to Ringer's, indomethacin had no effect, BW755c caused vasodilation, and ketoconazole caused vasoconstriction, which suggests that cytochrome P450 products might be vasoactive mediators in injured tissue. Therefore, purified synthetic compounds were compared with known vasodilators. The potency was prostacyclin greater than 12R-hydroxyeicostetraenoic acid greater than adenosine = 5,6 epoxyeicosatrienoic acid greater than AA, which supports the hypothesis that AA can be the source of a novel class of nonprostaglandin vasodilator compounds. In addition, at least one of the vasodilator responses was stereospecific. Nevertheless, the exact explanation for the differential effects of AA inhibitors on postburn blood flow is unknown.
Subject(s)
Arachidonic Acids/antagonists & inhibitors , Arachidonic Acids/metabolism , Burns/physiopathology , Mouth Mucosa/blood supply , Vasodilator Agents/pharmacology , 4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine , Animals , Arterioles/drug effects , Cheek/blood supply , Cheek/drug effects , Cheek/injuries , Cricetinae , Cytochrome P-450 Enzyme System/pharmacology , Indomethacin/pharmacology , Ischemia/physiopathology , Ketoconazole/pharmacology , Male , Mouth Mucosa/drug effects , Mouth Mucosa/injuries , Oxidation-Reduction , Pyrazoles/pharmacology , Regional Blood Flow/drug effects , Vasoconstriction/drug effects , Vasodilation/drug effectsABSTRACT
Silastic has been used with varying success as an implant material. When Silastic implants fail, migration and infection are generally involved. Perforation of the implant has been suggested as a means of minimizing implant migration. In this study, 30 implants were studied in Rhesus monkeys. The implants varied in external geometric form and in perforation size. Results indicated that fibrous encapsulation of the implant occurred within two weeks. The tissue readily invaded the perforations regardless of pore size. The configuration of connective tissue was unaffected by external geometry. Perforated implants were less mobile than non-perforated implants, and implants with perforations of 3 mm or more contained dense fibrous connective tissue supported by an underlying vascular mesenchymal tissue not seen in implants with smaller pores. As a result of this study, we recommend that perforations be used in Silastic implants and that these perforations be at least 3 mm in diameter.
Subject(s)
Dermatologic Surgical Procedures , Prostheses and Implants , Silicone Elastomers , Animals , Female , Macaca mulatta , Time Factors , Wound HealingSubject(s)
Face/surgery , Prostheses and Implants , Silicone Elastomers , Humans , Models, Anatomic , Surgery, Plastic/methodsABSTRACT
This study of 308 patients demonstrated that reliance upon physical findings alone is not reliable for the diagnosis and the prediction of treatment success in TMJS. However, those patients who had a lengthened masseteric silent period on the EMG had a 93% success rate from treatment with occlusal splint and occlusal adjustment. In contrast, those patients who had a normal masseteric silent period on the EMG had only a 21 percent success rate from the same therapy.
Subject(s)
Electromyography , Temporomandibular Joint Dysfunction Syndrome/therapy , Adolescent , Adult , Child , Child, Preschool , Female , Humans , Male , Maxilla , Middle Aged , Periodontal Splints , Prognosis , Temporomandibular Joint Dysfunction Syndrome/diagnosis , Temporomandibular Joint Dysfunction Syndrome/etiologyABSTRACT
We present a young patient in whom a large soft tissue defect of the neck was repaired with a deltoscapular flap.
