Effect of Platinum Nanoparticles on the Structure of Protein Molecules and Regulation of the Tone of Brain Vessels in Experimental Animals.

The effects of platinum nanoparticles on the morphological structures of the solid phase of blood serum and the tone of cerebral microvessels as indicators of the dynamics of homeostasis were studied on outbred albino male rats (n=40) weighing 300-350 g. Platinum nanoparticles were injected to experimental animals in 1 ml of physiological saline. For systemic BP measurements and blood sampling, the femoral artery was isolated and catheterized. The study of solid phase structures of blood serum was conducted by the method of cuneiform dehydration. The results showed that injection of platinum nanoparticles significantly affected the body of experimental animals.

A Marker of Cerebral Ischemia in Solid State Structures of Blood Serum.

Reversible cerebral ischemia of medium severity was reproduced in male Wistar rats by bilateral occlusion of the common carotid arteries. Solid-phase structures (anisomorphons) were obtained by marginal dehydration of the serum. Small focal isotropic defects in the serum anisomorphon texture were found in 100% cases during occlusion of the carotid arteries. Similar signs were detected in all patients with chronic cerebral ischemia, which proved specificity of this morphological marker of the disease.

Evaluation of Serum Reaction to Low-Intensity Laser Exposure by Its Solid Phase Structure.

The effects of various modes of low-intensity laser exposure on the sera of healthy subjects and patients are studied. It is shown by the cuneiform dehydration method that the serum structure is liable to change in response to certain modes of low-intensity laser exposure. The structure of patients' sera is more sensitive to low-intensity laser exposure.

Blood serum structures integrated assessment of pathophysiological changes in experimental cerebral ischemia.

The purpose: The purpose. To reveal solid-phase structures of blood serum during and after experimental cerebral ischemia. Methods: Integrated estimation pathophysiological changes at brain ischemia received on solid-phase structures of blood serum (BS) at 27 rats-male Vistar in weight of 300-390 g. Reversible brain ischemia of moderate severity was reproduced imposing surgical clips on both common carotids for 15 minutes then clips removed and provided bloodstream restoration on carotids. Blood took away in Eppendorf`s test tube in volume on 1 ml before imposing clips, directly ahead of their removal and in 30 minutes after bloodstream restoration. By the Method cuneiform dehydration received BS facia (dry a film) which structures analyzed by means of stereomicroscope MZ12 firm «Leica¼. Results of researches fixed on structural parameters BS before imposing clips, after removal clips and in 30 minutes after bloodstream restoration. Results: Before ischemia the integrated picture facias BS rats had the harmonious system organisation: the radial or partially-radial arrangement of the cracks, accurately generated konkrecias that testified to normal physiological condition of experimental animals. During the period occlusions carotids there were markers of a ischemia, activation of a capillary blood-groove, inflammatory reaction and stress. Partially these signs remained at reperfusion. Conclusion: The conducted researches of structures BS on experimental animals have allowed to reveal marker structures BS similar revealed at inspection of people with an ischemia of a brain.

[Morphological marker of tumor progression in laryngeal cancer].

Dynamic observation of 32 patients with laryngeal cancer, treated with radiotherapy and combination therapy, was performed using diagnostic technology "Litos-system", including the definition of serum morphotypes by marginal dehydration of biological fluids. In nine of them, on the background of the treatment, there was continued growth of the tumor. In the blood serum seven of nine patients had special microspheroliths, characterized by the presence of "wavy" structures. Such "wavy" microspheroliths could be seen as a harbinger of the continued growth of the tumor, because after their appearance there were detected signs of clinical progression. Apparently, the appearance of mentioned "wavy" structures in the patient's blood is a result of release into the blood of qualitatively new macromolecules produced by a clone of malignant tumor cells.

[Current views of etiopathogenetic mechanisms underlying the development of chronic pyelonephritis in subjects of different age].

A literature review of the issues pertinent to etiology and pathogenesis of chronic pyelonephritis is presented. Different aspects of pathogenesis of the disease in selected age groups of the patients (from childhood to senility) are considered and analysed with special reference to the etiology of acute and chronic pyelonephritis as well as the risk factors of this disease.

[Peculiarities of diagnostics of weakly symptomatic clinical course of chronic pyelonephritis].

The difficulty of early diagnostics of weakly symptomatic clinical course of chronic pyelonephritis (CP) is attributable to the lack of adequate methods. We used a novel Litos system diagnostic technology based on morphological analysis of dehydrated biological fluid The aim of the study was to develop methods for CP diagnostics from morphological characteristics of urine at different stages of the disease. It included 108 patients aged 58+/-1.3 yr with CP and 24 practically healthy subjects. Standard examination of all patients was supplemented by the analysis of urine using the Litos system. It was shown that morphological picture of urine makes it possible to objectively detect not only complete remission or exacerbation of the disease but also partial remission and high risk of CH even in patients with weakly symptomatic clinical course of this pathology. In case of incomplete remission, the treatment requires adequate long-term (three-course) antibacterial therapy despite normal results of urine analysis and microbiological studies. Patients at high risk of CP need to be prescribed preventive phytotherapy.

[The marker of discogenic diseases of the nervous system in the cerebrospinal fluid].

Using a novel diagnostic technology that allows to investigate the structure of a biological fluid formed during its phase transition into a dried film, we revealed a cause of mistaken results of protein concentrations in the cerebrospinal fluid of patients with discogenic radiculitis. The traditional laboratory study does not reveal the elevated content of protein in patients with discogenic radiculitis and hernia of invertebral discs due to its more active binding with salts with the following sedimentation during centrifugation. It can be explained by the involvement of salt crystals in the formation of the inert organic-mineral aggregate with protein molecules which structure was changed by dystrophy, ischemia, hypoxia, mechanic damage, tumor process. The aggregate is characterized by abnormally tight links. This phenomenon is known as biomineralization, the universal mechanism preventing the organism from toxic effects of products of degraded tissues.

[Morphological changes in blood plasma of patients with infectious endocarditis].

The aim of the study was to examine the morphological picture of sera obtained from 34 patients with infectious endocarditis (IE) pre-, intra- and postoperatively after their cuneiform dehydration. The method allowed integral homeostasis to be assessed including detection of markers of various pathological processes (onset of generalization of Candida infection and development of polyorgan dysfunction). Results of surgical treatment of IE for the period of 2002-2007 are presented.

[Microbiocenosis of oral cavity in patients from different age groups].

Worsening of state of oral cavity is observed not only in elderly persons, but also in adults aswell as in adolescents and children. The leading role in development of dental caries and periodontal diseases belongs to microorganisms. Oral cavity bacterial landscape was determined in adolescents, adults and elderly people with normal periodont or with gingivitis or periodontitis. The study showed that species and qualitative composition of oral cavity microflora were different in different age groups. In patients with normal periodont, non pathogenic (common commensals) microbial composition was revealed. In abnormal state of oral cavity, its microflora was characterized by the presence of high titers of potentiality pathogenic microorganisms.

[Treatment of chronic tonsillitis: prognostic criteria of efficacy].

An original method of wedge dehydration of biological fluids assessing the ability for separation of organic and mineral constituents was for the first time used in patients with different forms of chronic tonsillitis (CT). The method was used in 102 patients aged 8-68 years with chronic inflammation of the palatine tonsils out of exacerbation. A dehydrated drop of tonsillar lacuna discharge (TLD), facia, was studied morphologically under microscope at small magnification (x10--x50). Three types of TLD facia were identified in CT patients. These types characterized severity of the pathological process in the palatine tonsils. Changes of facia type in patients with different CT forms were compared. Basic regularities in the disease progress were determined. This enabled prognosis of a further course of CT in an individual patient and, therefore, planning treatment policy for each case.

Diagnostic markers in the structures of human biological liquids.

INTRODUCTION: To date, morphology research in laboratory diagnostics has referred only to cell tissues and excluded biological fluids. Our new technology is based on the study of structures of biological fluids formed during transition from liquid to solid state by dehydration under special conditions. These structures are formed due to the special "mosaic" configuration of molecules and microaggregates of the substance (protein and others) dissolved in the studied fluids. It should be considered as well that the specific structure of protein molecules is a fundamental basis of anatomical and physiological peculiarities of an organism. All pathological processes are initiated by the changes in the spatial configuration of protein molecules. METHODS: We have developed a special method named "cuneiform dehydration of biological liquids" to obtain thin films which possess specific structures. During the transition of a biological liquid into a solid, organic molecules build specific macrostructures in conformity with their configuration. RESULTS: Studies of the solid phase structures of different biological liquids allow us to find specific markers of various pathological processes in the human organism. In blood serum, we discovered markers of inflammation, sclerosis, chronic intoxication; in tear--markers of different stages of glaucoma; and in synovial fluid--markers of different stages of osteoarthrosis. In our investigation of 143 healthy people and 1,419 patients, a high rate of compliance of the discovered markers with the results of routine clinical and laboratory methods were observed. As the suggested technology has a higher sensitivity, the results obtained by this technology and routine methods do not always coincide. CONCLUSION: The developed technology gives new information about the state of the human organism and may be used in practice for diagnostics of various pathological processes and at pre-clinical studies of their development, as well as for the evaluation of the efficacy of medical treatment.

[Morphological structure of oral liquid--diagnostic possibilities].

This research has revealed the morphological structure of the oral liquid obtained from 187 adolescents on the basis of self-organized process occurring during the phase transition "liquid-solid". The results of the research have been compared with the data obtained in the course of dental checkup. The parameters of the oral liquid morphological structure in case of healthy periodontium have been identified and the markers of pathology caused by periodontal tissue inflammation and caries have been determined.

Bio-liquid morphological analysis.

Information is presented on the new scientific line in medicine and biology: bio-liquid morphology. The interdisciplinary character of the given research area is emphasized. The problems and prospects of bio-liquid morphological analysis development both in applied and fundamental aspects are discussed.

[Express-methods in diagnostics of early stages of urolithiasis]. / Ekspress-metody v diagnostike rannikh stadii mochekamennoi bolezni.

A complex of methods for early diagnosis of urolithiasis is proposed. This complex includes ultrasonic investigation of the kidneys and new diagnostic technique Litos-System which allow making diagnosis before formation of a renal calculus.

[Diagnosis, pharmacologic therapy and prevention of urolithiasis]. / Diagnostika, medikamentoznoe lechenie i profilaktika mochekamennoi bolezni.

The article reviews present-day pathogenetic and diagnostic conceptions and methods of diagnosis of urolithiasis. Its medication and prevention methods are outlined.

[A morphological pattern of the aqueous humor in the evaluation of pathophysiological mechanisms of involutional cataract genesis]. / Morfologicheskaia kartina vodianistoi vlagi v otsenke patofiziologicheskikh mekhanizmov involiutivnogo kataraktogeneza.

The method of wedge-type dehydration was made use of to study the specificity of the systemic structure of 496 samples of aqueous humor obtained in the course of surgical interventions in 62 patients for senile cataract. The results were analyzed with respect to a cataract stage and it was established that the systemic structure of aqueous humor reflects different-phase impairments in the system of the intermolecular interaction of aqueous-humor components, which is mainly related with activated processes of organic products' mineralization as observed in senile cataract progression.

[Diuress for the treatment of early stages of urolithiasis]. / Diuress dlia lecheniia rannikh stadii urolitiaza.

The efficacy of the plant diuretic Diuress is evaluated by using the rapid diagnosis program including renal ultrasonography, Lithos system, and urinary sediment microscopy.