Subject(s)
Gene Expression Regulation, Viral , Herpesvirus 8, Human/physiology , Valproic Acid/pharmacology , Virus Activation , Butyrates/pharmacology , Herpesvirus 8, Human/drug effects , Herpesvirus 8, Human/genetics , Humans , Tumor Cells, Cultured , Virus Activation/genetics , Virus Replication/drug effectsABSTRACT
The effect of amitraz, a formamidine insecticide, on in vitro intestinal contractions was studied in teh transmurally-stimulated guinea-pig ileum. An electrical stimulation (with 80 V/0.5 msec/0.1 Hz shown on the dial of the stimulator) caused the ileum to contract presumably via the release of acetylcholine. Amitraz (10(-7) to 10(-6) M) produced a dose-dependent inhibition of these transmurally-stimulated contractions. This effect of amitraz was blocked and reversed by idazoxan (10(-6) M), an alpha 2-adrenoceptor antagonist, but was not prevented by prazosin (10(-6) M), an alpha 1-adrenoceptor antagonist. These results suggest that alpha 2-adrenoceptors mediate the effects of amitraz on the transmurally-stimulated guinea-pig ileum. The results also suggest that amitraz decreases intestinal contraction by activating the alpha 2-adrenoceptors in the myenteric (Auerbach's) plexus, thus inhibiting parasympathetic tone.
Subject(s)
Gastrointestinal Motility/drug effects , Ileum/drug effects , Insecticides/pharmacology , Toluidines/pharmacology , Adrenergic alpha-Agonists/physiology , Animals , Depression, Chemical , Dioxanes/pharmacology , Dose-Response Relationship, Drug , Guinea Pigs , Idazoxan , Ileum/physiology , Prazosin/pharmacology , Toluidines/administration & dosage , Toluidines/antagonists & inhibitorsABSTRACT
Production of CO2, fatty acids and glycerol from glucose and acetate was measured in slices of liver and adipose tissue taken from mature dogs. Acetate was the predominant carbon source for de novo fatty acid synthesis in both tissues. Fatty acid synthesis occurred at greater rates in adipose tissue than in liver. Glucose provided carbon for glycerol synthesis production in adipose tissue. Results support the concept that adipose tissue, and not liver, is the principal anatomical site for fatty acid synthesis in dogs.
