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1.
Bull Exp Biol Med ; 176(1): 101-104, 2023 Nov.
Article in English | MEDLINE | ID: mdl-38085397

ABSTRACT

The pharmacological activity of granulocyte CSF (G-CSF) immobilized using electron-beam synthesis nanotechnology (imG-CSF) was evaluated in an experimental model of ovarian reserve depletion. The effectiveness of the drug was compared with that of its unmodified form. Depletion of the ovarian follicular pool in female Sprague-Dawley rats was caused by a single intravenous injection of the antitumor drug etoposide in the maximum tolerated dose. The effectiveness of the studied drugs was assessed by serum concentration of anti-Mullerian hormone (AMH) measured by ELISA and by the number of primordial, two-layer, multilayer, and atretic follicles counted on serial sections of the ovaries (5-µm thick; through the entire organ) stained with hematoxylin and eosin. It was found that imG-CSF prevents depletion of the ovarian reserve in the model used, which was confirmed by high AMH concentration and higher numbers of primordial, two- and multilayer follicles in comparison with the corresponding parameters in the control (etoposide), and by a decrease in the severity of atretic processes. Unmodified form of the drug demonstrated lower efficiency.


Subject(s)
Ovarian Reserve , Rats , Animals , Female , Etoposide , Granulocyte Colony-Stimulating Factor/pharmacology , Electrons , Rats, Sprague-Dawley , Anti-Mullerian Hormone , Models, Theoretical
2.
Bull Exp Biol Med ; 175(4): 503-507, 2023 Aug.
Article in English | MEDLINE | ID: mdl-37775703

ABSTRACT

We studied ante- and postnatal development of the offspring of intact female rats crossed with males injected with low doses of methotrexate 3 and 6 months before mating. The time of crossing corresponded to the manifestation of the cytostatic effect on spermatogonial stem cells. The offspring of methotrexate-treated males was characterized by increased preimplantation losses and fetal growth restriction in the antenatal period and inhibition of physical development, delayed formation of sensory-motor reflexes, and impaired learning abilities in the postnatal period.


Subject(s)
Methotrexate , Prenatal Exposure Delayed Effects , Humans , Rats , Animals , Pregnancy , Female , Male , Methotrexate/pharmacology , Prenatal Exposure Delayed Effects/chemically induced , Reproduction , Learning , Reflex
3.
Bull Exp Biol Med ; 173(6): 714-718, 2022 Oct.
Article in English | MEDLINE | ID: mdl-36322303

ABSTRACT

We studied the effect of antioxidants dibornol (2,6-diisobornyl-4-methylphenol) and its derivative (4-hydroxymethyl-2,6-diisobornylphenol), members of the alkylated phenols group, on the redox potential of male germ cells and their morphological and functional state in the rat model of pathospermia. Pharmacological effect was observed in animals treated with dibornol. The studied compounds led to the normalization of the antioxidant-prooxidant balance. However, the value of this indicator against the background of treatment with dibornol derivative attested to a shift in the redox balance of cells towards reduction reactions.


Subject(s)
Antioxidants , Phenols , Male , Animals , Rats , Antioxidants/pharmacology , Antioxidants/therapeutic use , Antioxidants/metabolism , Reactive Oxygen Species , Oxidation-Reduction , Phenols/pharmacology , Phenols/therapeutic use , Germ Cells/metabolism
4.
Bull Exp Biol Med ; 173(3): 341-345, 2022 Jul.
Article in English | MEDLINE | ID: mdl-35852682

ABSTRACT

Delayed gonadotoxic effects were revealed in outbred male sexually mature rats (SD) after exposure to paclitaxel in the prepubertal period, and the possibility of their correction with p-tyrosol was shown. It was found, that administration of paclitaxel does not inhibit the ability of animals to conceive, but impairs the reserve capacity of the testicular tissue. In intact female rats crossed with male rats receiving paclitaxel, increased post-implantation fetal death was observed. Combined administration of paclitaxel and p-tyrosol alleviated the delayed effects of the cytostatic treatment on the prepubertal testis.


Subject(s)
Phenylethyl Alcohol , Testis , Animals , Female , Male , Paclitaxel/toxicity , Phenylethyl Alcohol/analogs & derivatives , Phenylethyl Alcohol/pharmacology , Rats
5.
Bull Exp Biol Med ; 172(5): 632-636, 2022 Mar.
Article in English | MEDLINE | ID: mdl-35352256

ABSTRACT

The regenerative properties of p-tyrosol were investigated in a model of testicular insufficiency caused by a toxic effect on spermatogonial stem cells (single administration of paclitaxel in the maximum tolerable dose). Against the background of p-tyrosol administration, we observed an increase in the number of normal spermatogonia and Sertoli cells, stimulation of spermatogenesis, and renewal of the spermatogenic tissue. The treatment with p-tyrosol also led to a decrease in DNA damage in cells of the testicular tissue. These changes were accompanied by a decrease in the level of free radicals, an increase in antioxidant protection, and normalization of the redox potential.


Subject(s)
Spermatogonia , Testis , Humans , Male , Phenylethyl Alcohol/analogs & derivatives , Spermatogenesis , Stem Cells
6.
Bull Exp Biol Med ; 171(1): 37-40, 2021 May.
Article in English | MEDLINE | ID: mdl-34050414

ABSTRACT

The morphological and functional state of the reproductive system was studied in male outbred rats (SD stock) and male F1(CBA×C57BL/6) mice after long-term (3 months) methotrexate administration. The drug was administered subcutaneously once a week for 4 weeks, the dose for male rats was 1 mg/kg, for male mice 2.2 mg/kg. It was found that male rats retained the ability to conceive, their reproductive potential was not limited by increased risk of embryo death. At the same time, signs of astheno- and pathospermia were revealed. The testicular tissue was characterized by reduced content of the sources of the proliferative pool of spermatogenesis. In mice treated with methotrexate, increased content of DNA breaks was detected in the testicular cells.


Subject(s)
Methotrexate , Spermatogenesis , Animals , Male , Methotrexate/toxicity , Mice , Rats , Reproduction , Testis
7.
Bull Exp Biol Med ; 163(2): 214-217, 2017 Jun.
Article in English | MEDLINE | ID: mdl-28726202

ABSTRACT

We studied possible toxic effects of antiviral drug Kagocel on reproductive function in pubertal male rats. The drug was administered in therapeutic and 10-fold higher doses throughout the spermatogenesis cycle (48 days). Kagocel did not reduce mating and fertilizing capacities, did not suppress spermatogenesis, and had no toxic effects on the offspring. The results characterize Kagocel as a drug with a broad reproductive safety profile and demonstrate that the age limits for using Kagocel in pediatric practice can be extended.


Subject(s)
Antiviral Agents/adverse effects , Gossypol/adverse effects , Spermatogenesis/drug effects , Animals , Male , Puberty/drug effects , Rats , Rats, Sprague-Dawley , Sexual Behavior, Animal/drug effects , Testis/drug effects
8.
Bull Exp Biol Med ; 161(5): 683-686, 2016 Sep.
Article in English | MEDLINE | ID: mdl-27709383

ABSTRACT

Effectiveness of the granulocyte colony-stimulating factor immobilized by using electronbeam synthesis nanotechnology was investigated on the model of experimental testicular failure caused by the toxic effect on stem spermatogonia. Administration of the drug to experimental paclitaxel-treated animals increased the number of sources of the proliferative pool of spermatogenesis and its productivity. The effectiveness of immobilized granulocyte colony-stimulating factor was based on its ability to stimulate reparative regeneration of the spermatogenic tissue, which manifested in a decrease in spermatogenic layer maturity and increase in the number of microenvironment cells. Effectiveness of the immobilized form of the drug was superior to that of non-immobilized form.


Subject(s)
Granulocyte Colony-Stimulating Factor/pharmacology , Immobilized Proteins/pharmacology , Infertility, Male/drug therapy , Spermatogonia/physiology , Animals , Antineoplastic Agents/adverse effects , Drug Evaluation, Preclinical , Granulocyte Colony-Stimulating Factor/therapeutic use , Immobilized Proteins/therapeutic use , Infertility, Male/chemically induced , Male , Nanotechnology , Paclitaxel/adverse effects , Rats, Wistar , Regeneration , Spermatogenesis , Spermatogonia/drug effects
9.
Bull Exp Biol Med ; 158(4): 513-6, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25708337

ABSTRACT

A course of dihydroquercetin (antioxidant) injections to 5-month-old Wistar rats with sulpiride-induced benign prostatic hyperplasia led to reduction of proliferative activity in the glandular structures and to attenuation of the inflammatory reaction in the tissue. Prostatic antioxidant/prooxidant balance returned to normal after the treatment.


Subject(s)
Antioxidants/metabolism , Prostatic Hyperplasia/chemically induced , Prostatic Hyperplasia/drug therapy , Prostatic Hyperplasia/pathology , Quercetin/analogs & derivatives , Reactive Oxygen Species/metabolism , Sulpiride/adverse effects , Animals , Histological Techniques , Male , Quercetin/administration & dosage , Quercetin/metabolism , Quercetin/pharmacology , Rats , Rats, Wistar , Statistics, Nonparametric
10.
Bull Exp Biol Med ; 155(1): 67-70, 2013 May.
Article in English | MEDLINE | ID: mdl-23667874

ABSTRACT

Comparative evaluation of the efficiency of prostatotropic agents was carried out in rat experiments. Serenoa repens plant preparation and polypeptides isolated from the cattle prostate were used for the treatment of benign hyperplasia. Drugs in parallel with sulpiride similarly led to shrinkage of the acinar epithelial area and to emergence of a trend to an increase of the stromal/epithelial proportion, more so after Serenoa repens treatment.


Subject(s)
Peptides/therapeutic use , Phytotherapy , Plant Extracts/therapeutic use , Prostatic Hyperplasia/drug therapy , Animals , Cattle , Male , Prostate , Prostatic Hyperplasia/chemically induced , Rats , Serenoa , Sulpiride
11.
Bull Exp Biol Med ; 143(5): 614-6, 2007 May.
Article in English | MEDLINE | ID: mdl-18239783

ABSTRACT

The possibility of reducing the ovariotoxicity of antitumor drug etoposide with buserelin, a hypothalamic regulator of pituitary function, was studied in female Wistar rats. Quantitative analysis of ovarian structural and functional elements on serial sections through the entire organ showed that 3 months after combined treatment with etoposide and buserelin, the morphological picture of the ovarian glands did not differ from that in intact animals of the same age, while etoposide monotherapy led to earlier development of atrophic processes. Six months after treatment, the number of bi- and multilayer follicles was significantly higher in rats receiving combined therapy compared to animals treated with etoposide alone.


Subject(s)
Antineoplastic Agents, Phytogenic/toxicity , Buserelin/pharmacology , Etoposide/toxicity , Fertility Agents, Female/pharmacology , Ovary/drug effects , Animals , Female , Rats , Rats, Wistar
12.
Bull Exp Biol Med ; 141(5): 578-80, 2006 May.
Article in English | MEDLINE | ID: mdl-17181057

ABSTRACT

Progeny of Wistar rats treated with vepesid 1, 3, and 6 months before mating is characterized by common pathological changes. These changes were more pronounced and more diverse in animals descending from females receiving the cytostatic compared to the progeny of treated males. The severity of toxic effects depended on the period between mating and vepesid treatment. Cytostatic treatment 3 months before mating was associated with minimum toxicity for the progeny.


Subject(s)
Abnormalities, Drug-Induced , Antineoplastic Agents, Phytogenic/toxicity , Etoposide/toxicity , Prenatal Exposure Delayed Effects , Animals , Animals, Newborn , Female , Fetal Death/chemically induced , Male , Pregnancy , Rats , Rats, Wistar , Time Factors , Topoisomerase II Inhibitors
13.
Bull Exp Biol Med ; 140(3): 317-9, 2005 Sep.
Article in English | MEDLINE | ID: mdl-16307047

ABSTRACT

Experiments on adult rats showed that a single intravenous injection of antitumor drug vepeside in a MTD (maximum tolerable dose) reduced the reproductive status during periods corresponding to exposure of mature sex cells, spermatocytes, and spermatogonia in male rats and exposure of oocytes in ovulating, mature, and primordial follicles in female rats. Reduction of the male and female reproductive function manifested in increased antenatal mortality of the progeny. The toxic effects of the drug on mature male sex cells caused temporary partial infertility.


Subject(s)
Etoposide/pharmacology , Reproduction/drug effects , Animals , Female , Fertility/drug effects , Fertilization/drug effects , Fetal Death , Male , Oocytes/drug effects , Pregnancy , Pregnancy Rate , Rats , Spermatozoa/drug effects
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