ABSTRACT
In many diseases associated with impairments in iron metabolism, erythrocytes exhibit an increased sensitivity to oxidative stress induced in vitro. In this study, we have examined the antioxidant status of erythrocytes from healthy donors and from 12 patients with disorders of iron homeostasis by measuring the extent of t-BHP-induced hemolysis in vitro. The extent of hemolysis observed with patient erythrocytes was significantly higher than that observed in experiment with normal cells. After therapeutic infusions of the antioxidants mexidol or emoxypin, oxidative hemolysis in patients was restored to normal values and blood hepcidin content increased significantly. A significant correlation was observed between hepcidin concentration after treatment and t-BHP-induced hemolysis before treatment. These data suggest that antioxidants may exert a favorable effect under pathological conditions associated with iron overload disease.
Subject(s)
Antioxidants/therapeutic use , Erythrocytes/metabolism , Hepcidins/blood , Iron Metabolism Disorders/drug therapy , Picolines/therapeutic use , Antioxidants/administration & dosage , Antioxidants/metabolism , Female , Hemolysis/drug effects , Humans , Infusions, Intravenous , Iron/blood , Iron Metabolism Disorders/blood , Male , Middle Aged , Picolines/administration & dosage , Treatment OutcomeABSTRACT
We have previously proposed the osmofiltration method based on a modified Hanss hemorheometer to analyze distributions of erythrocytes in their ability to pass through membrane filters with 3 microns pores. Upon decrease in medium osmolality (u) the erythrocyte volume increases. When cell volume becomes V = Vcr at u = ucr, such cell loses its ability to pass through a 3 microns pore. The flow rate of erythrocyte suspension containing cells with different ucr through a filter gradually decreases with decreasing medium osmolality. This rate becomes zero at some u = omega, when the number of non-filterable cells in the applied sample approaches the number of pores in filter. Experimental determination of the dependencies of the filtration rate on medium osmolality for various hematocrit values allows to obtain omega for each hematocrit and, thereby, to assess the distribution of erythrocytes in ucr. Here, we propose a simplified version of this method, which allows screening of the erythrocytes in heterogeneous suspensions for the distribution in ucr by measuring omega for only two hematocrit values, 0.1% and 1%. Applications of the proposed method are exemplified by analysing the erythrocyte populations of healthy donors, of patients with microspherocytosis, hemochromatosis and normal erythrocyte populations in an acidic environment.
Subject(s)
Erythrocyte Deformability , Hemorheology , Erythrocyte Volume , Hematocrit , Hemochromatosis/blood , Hemofiltration , Humans , Hydrogen-Ion Concentration , Micropore Filters , Osmolar Concentration , Spherocytosis, Hereditary/bloodABSTRACT
AIM: To establish changes of and correlations between erythrodieresis and blood rheology in hereditary hemochromatosis (HHC). MATERIALS AND METHODS: Hemorheologic indices (asymptomatic blood and plasma viscosity, that of blood at low shift speed, red cell deformity and aggregation) were studied in 42 HHC patients and 23 healthy donors. The patients were divided into groups by homozygous (groups 1 and 2) or heterozygous (groups 3 and 4) allel genes and sex (females-groups 2 and 4, males-groups 1 and 3). RESULTS: All the patients had increased resistance of red cells in high percentage of their high-resistance populations (correlation index 0.8-0.9) due to destruction and elimination from the circulation. These changes were more pronounced in males, similar in homo- and heterozygotes. The presence in the peripheral blood of red cells with high resistance is associated with a 25-30% decrease in the red cell rigidity, in groups 1 and 3 this decrease reached 30-40%. To a certain degree high deformity and aggregation of red cells is explained by high plasma viscosity (correlation index 0.4). Plasma viscosity was high in all the patients of all groups. CONCLUSION: HHC runs with rejuvenascence of erythroid cells. Changed activity of erythrodieresis and damage to red cells at the level of microcirculation may be involved in iron metabolism disturbance and development of tissue hemosiderosis.
Subject(s)
Blood Viscosity , Hemochromatosis/blood , Hemochromatosis/genetics , Hemolysis/physiology , Adolescent , Adult , Aged , Aged, 80 and over , Erythrocytes/physiology , Female , Genotype , Hemochromatosis/physiopathology , Humans , Male , Microcirculation , Middle AgedSubject(s)
Anion Exchange Protein 1, Erythrocyte/metabolism , Erythrocyte Membrane/metabolism , Hemochromatosis/blood , Spectrin/metabolism , Adolescent , Adult , Aged , Calorimetry, Differential Scanning , Child , Erythrocyte Membrane/ultrastructure , Freeze Fracturing , Hemochromatosis/genetics , Hemochromatosis/pathology , Humans , Male , Middle AgedABSTRACT
Hyperferremia is shown to affect antioxidant system of the body, oxidation-reduction reactions in the cells seen as shifts in lymphocyte chemiluminescence. Dynamic changes in lymphocyte chemiluminescence reflect the level of hyperferremia.
Subject(s)
Iron/blood , Lymphocytes/metabolism , Antioxidants , Combined Modality Therapy , Deferoxamine/therapeutic use , Dose-Response Relationship, Drug , Ferritins/pharmacology , Free Radicals/blood , Hemochromatosis/blood , Hemochromatosis/therapy , Humans , Iron/pharmacology , Luminescent Measurements , Lymphocyte Activation/drug effects , Lymphocytes/drug effects , Myelodysplastic Syndromes/blood , Myelodysplastic Syndromes/therapy , Plasmapheresis , Transferrin/analysisSubject(s)
Erythrocyte Membrane/physiology , Hemochromatosis/blood , Adolescent , Adult , Calorimetry/methods , Female , Hemochromatosis/genetics , Humans , Male , Middle AgedSubject(s)
Hemochromatosis/drug therapy , Hemosiderosis/drug therapy , Iron Chelating Agents/therapeutic use , Iron/antagonists & inhibitors , Administration, Oral , Animals , Drug Carriers , Hemochromatosis/metabolism , Hemosiderosis/metabolism , Humans , Iron/metabolism , Iron Chelating Agents/administration & dosage , Liposomes , Mice , Rabbits , RatsSubject(s)
Blood Banks , Blood Transfusion/instrumentation , Adult , Equipment Design , Humans , Middle Aged , Polymers , USSRSubject(s)
Arterial Occlusive Diseases/therapy , Leg/blood supply , Plasma Substitutes/therapeutic use , Animals , Arteries , Blood Viscosity/drug effects , Collateral Circulation/drug effects , Dextrans/therapeutic use , Hemodynamics/drug effects , Humans , Intraoperative Care , Molecular Weight , Postoperative Care , Povidone/therapeutic use , Regional Blood Flow/drug effects , Thrombosis/therapyABSTRACT
To treat patients with chronic arterial occlusion of the lower extremities, the authors used 3% solution of low molecular polyvinyl alcohol in 0.9% sodium chloride solution-polydese. The substance was injected intravenously and intraarterially in a dosage of 10 ml per k Kg of weight. There was noted a good tolerance of the drug, and also the absence of toxic, pyrogenic and allergic reactions. In polydese therapy a marked improvement of the peripheral blood circulation was observed. The conducted clinical observations, laboratory studies have proved polydese to be the drug of a positive rheological effect.