Two new frog species from the Litoria rubella species group from eastern Australia.

The bleating tree frog (Litoria dentata) is one of the more prominent pelodryadid frogs of eastern Australia by virtue of its extremely loud, piercing, male advertisement call. A member of the Litoria rubella species group, L. dentata has a broad latitudinal distribution and is widespread from coastal and subcoastal lowlands through to montane areas. A recent mitochondrial DNA analysis showed a deep phylogeographic break between populations of L. dentata on the mid-north coast of New South Wales. Here we extended the mitochondrial survey with more geographically comprehensive sampling and tested the systematic implications of our findings with nuclear genome wide single-nucleotide polymorphism, morphological and male advertisement call datasets. While similar in appearance and in male advertisement call, our integrative analysis demonstrates the presence of three species which replace each other in a north-south series. We redescribe Litoria dentata, which is restricted to coastal north-eastern New South Wales, and formally describe Litoria balatus sp. nov., from south-eastern Queensland, and Litoria quiritatus sp. nov., from the mid-coast of New South Wales to north-eastern Victoria.

Derivation of clinically applicable schwann cells from bone marrow stromal cells for neural repair and regeneration.

Schwann cells are critically important for tissue repair, axonal regrowth and remyelination following injury to peripheral nerves. The absence of Schwann cells or an equivalent cell type in the central nervous system (CNS) may limit the regeneration capacity of the CNS. Mesenchymal stem cells (MSCs) have therefore been investigated for their potential to be induced to develop a Schwann cell phenotype. The methods for derivation of Schwann cell-like cells from MSCs and the benefits and limitations of each of these methods are presented in this review. Issues related to instability of the derived Schwann cell phenotype, apoptosis of derived cells in transplants, and the inability to predict with confidence how the cells will behave after transplantation are discussed. Finally, we suggest the need for further elucidation of the biology of Schwann cell differentiation and the signals for their derivation from MSC, in order to resolve these obstacles and to enable transplantation of MSC-derived Schwann cells as a therapeutic strategy in CNS injury.

Perspective: Economically speaking about nurse staffing.

Evidence exists telling us that we are currently in the early stages of a nursing shortage, and at the same time consumer demands for health care continues to increase. Principals of economics, when applied to such a state forewarn of changing times and can help us to predict a future very different that what we now see.

The requirement for natural sunlight to prevent vitamin D deficiency in iguanian lizards.

To investigate possible causes of embryonic and neonatal mortality in a group of captive Fijian iguanas (Brachylophus fasciatus and Brachylophus vitiensis), the vitamin D status of adults in the colony was compared with that of agamid and iguanid lizards either housed in indoor enclosures under artificial ultraviolet light or exposed to natural sunlight (wild-caught or captive animals housed outdoors). Those under artificial lighting had a significantly lower vitamin D status than those housed exclusively outdoors, whereas the vitamin D status of Fijian iguanas that had received intermittent exposure to natural sunlight was intermediate and not significantly different from that of animals housed exclusively outdoors. However, eggs from some of these Fijian iguanas had substantially lower vitamin D content than eggs from outdoor iguanid and agamid animals. Artificial ultraviolet light, therefore, might not be an adequate substitute for natural sunlight to maintain vitamin D status of lizards. This possible inadequacy may be because either artificial ultraviolet light has a lower intensity of the wavelengths that induce vitamin D than does sunlight or the intensity of the artificial lighting is not sufficient.

An examination of plantar pressure measurements to identify the location of diabetic forefoot ulceration.

The purpose of this study was to evaluate pressure-time parameters on the sole of the foot in order to predict sites of pathology in diabetics. Mid-gait walking steps from 36 subjects diagnosed with diabetes and ulceration to the forefoot were analyzed using the Pedar in-shoe pressure analysis system. The time that weight was loaded on the hallux and lesser digits was found to be significantly shorter on the feet with ulceration compared to those without. A combination of five pressure measure reports could significantly differentiate between the three common locations of forefoot ulcerations. The significant pressure measure reports were the pressure-time integral measured over the whole foot and pressure-time integral and peak pressure measured separately at the first and combined lesser metatarsophalangeal joint regions. A preliminary model was developed using discriminate analysis that was accurate to 72.7% in identifying the location of the subjects' ulceration. Sensitivity and specificity formulas were calculated to demonstrate the diagnostic value of the model. The model accurately identified ulceration locations to a sensitivity of 83% and a specificity of 69%. Further refinement and testing is underway to refine the model to develop a screening tool to identify the site of potential foot ulceration and to prevent it and its sequelae of lower limb ulceration in the diabetic population.

Use of a computerized digital camera in podiatric medical practice.

Multimedia technology was once rarely found outside the realm of commercial production studios or in elaborate computer games. However, with the addition of only a few simple accessories, recent advances have made this technology readily available to the podiatric medical practitioner on a desktop office computer. The role that the application of multimedia technology using a computerized digital camera can play in a podiatric medical practice--including in such areas as record keeping, outcome measurement, patient education, interdisciplinary communications, and practice-management tools--is discussed.

The distribution and identification of dangerously venomous Australian terrestrial snakes.

The identification of dangerous Australian snakes is important in instituting therapy for envenomation. Despite the availability of a number of identification guides with varying degrees of generality, identification can be problematic for several reasons. These include a diversity of common names, many of which are inappropriate or regionally applied to different species, identification keys that focus on variable features, intraspecific variation and interspecific convergence in colouration, and recent changes in scientific nomenclature of species and genera. Geographic distribution of the dangerously venomous species can be a useful aid to identification, by limiting the range of options in a region. However, delineation of the limits of distribution relies on fine scale mapping beyond the resolution of most identification guides. This article provides a summary of the geographic limits of the dangerously venomous Australian snakes, with particular emphasis on major population centres, and clarifies some problems in identification, particularly among brown-coloured snakes.

Instrumentation considerations of a clinical and a computerized technique for the measurement of foot angles.

Measurement of the foot angles either directly from the patient, from video images, or from radiographs is integral to podiatric clinical practice to confirm diagnoses and to plan, prescribe, and monitor treatment protocols. The reliability, precision, and accuracy involved in any measured value limits the validity and usefulness of the measurement to optimal patient management. Studies are described that ensured the accuracy and validity of the standard clinical tool, the universal goniometer (UG), by applying a calibration protocol. These same calibration angles were then measured by a computer-assisted human movement analysis system, the Ariel Performance Analysis System (APAS). The APAS was found to overestimate consistently the UG angular measures by less than 1 degree and this amount of error was considered clinically irrelevant. The angular results obtained by a clinician and a technician using the APAS on two separate days were tested and were found to be comparable and reliable to within 1 degree, and thus the analysis was deemed to be of excellent reliability and precision. The study found that clinicians could establish the accuracy and validity of their instruments by means of simple calibration, and that computer measures could be repeated on patients by a clinical or a technician. The simple calibration procedure described will assist the clinician to ensure that the measures obtained in the clinical setting have minimal measurement error and that the values can be confidently used to make decisions and draw clinical inferences.

Potency in groups: articulating a construct.

This paper examines potency, defined as the collective belief in a group that it can be effective, and its role in determining group effectiveness. The paper illustrates the construct and reviews its origins. The distinctiveness of potency from efficacy and from other collective and motivational constructs is described. The measurement of potency is also examined. The paper concludes with a presentation of a conceptual framework for understanding the determinants of potency and with a discussion of future theory, research and practice.

Histological and immunopathological analysis of recovered encapsulated allogeneic islets from transplanted diabetic BB/W rats.

Allogeneic islets encapsulated in an alginate/poly-L-lysine membrane and transplanted into diabetic BB/W rats resulted in graft failure within 2 weeks of transplantation. Graft failure was associated with a dense pericapsular infiltrate (PCI) that resulted in necrosis of the encapsulated islets. The PCI could be inhibited by immunosuppressive agents, including cyclosporine and dexamethasone, and this resulted in a significant increase in graft survival. Immunopathological characterization of the PCI indicated that there was a predominance of macrophages. T helper cells also appeared to be present in this PCI. Empty capsules were also found to induce a similar PCI that was identical in composition to that found around encapsulated islets. Thus alginate/poly-L-lysine capsules do not appear to be biocompatible and may account for the variable results in islet graft survival found with these capsules.

Transplantation of encapsulated allogeneic islets into diabetic BB/W rats. Effects of immunosuppression.

Allogeneic islets obtained from Lewis rats were transplanted into diabetic BB/W rats with or without cyclosporine. In addition, these islets were encapsulated in alginate-poly L-lysine membranes and then transplanted into diabetic BB/W rats with or without immunosuppressive and/or antiinflammatory agents. The agents used were cyclosporine, dexamethasone, indomethacin (Ind), or a combination of these. Our results show that islets alone survived for 7 days, with or without CsA therapy. Encapsulated islets survived for 14.2 days, and this was extended by CsA, Dex, or CsA + Ind. Loss of encapsulated graft functions was associated with formation of a dense pericapsular infiltrate, which was inhibited by CsA, Dex, CsA + Ind, or CsA + Dex. In addition, the infiltrate was reduced in animals that had diabetes for long periods of time (greater than 5 months versus less than 1 month). Empty capsules also provoked this cellular response. Thus, encapsulation of islets resulted in slightly prolonged islet survival, which was further enhanced by immunosuppression.

The fate of transplanted encapsulated islets in spontaneously diabetic BB/Wor rats.

The present study was undertaken to determine whether the transplantation of encapsulated MHC identical islets into diabetic BB/Wor/BB rats could cure their diabetes. Islets were isolated from diabetes-resistant BB/Wor/WB rats and encapsulated in alginate-polylysine-alginate membranes. Five thousand islets were transplanted into the peritoneal cavity of spontaneously diabetic BB/Wor/BB rats (n = 9) that had been insulin dependent for more than five weeks. Similar diabetic animals were transplanted with 5,000 unencapsulated islets (n = 10) or with empty capsules (n = 3). After transplantation of free islets, the animals reverted to the insulin-dependent state after a median of 16 days (17 +/- 4 days, mean +/- SEM). After transplantation of encapsulated islets, the animals reverted to the diabetic state after a median of 59 days (54 +/- 10 days, mean +/- SEM). Light microscopic and electron microscopic analyses of capsules recovered from the peritoneal cavity after failure of graft function showed no evidence either of lymphocytic invasion of the capsules or of specific destruction of the islets. The capsules were, however, overgrown by a layer of histiocytes and numerous layers of fibroblasts. Empty capsules recovered after 15 and 60 days were overgrown to the same extent.

Morphological and quantitative aspects of the development of cytoplasmic granules in concanavalin A-stimulated normal human lymphocytes.

We have studied normal human lymphocytes stimulated by concanavalin A to determine the rate and extent of development of cytoplasmic granules. Morphological studies on stimulated lymphocytes were undertaken at the light and electron microscopic level. The percentage of T lymphocytes bearing different CD antigens (CD3, CD4, and CD8) that also contained cytoplasmic granules was also assessed. Granules were identified in a high percentage of lymphocytes bearing each of the three CD markers studied.

Microencapsulated hepatocytes for bioartificial liver support.

Free hepatocytes, harvested from normal rat livers by portal vein collagenase perfusion, were encapsulated within alginate-polylysine membranes and served as a liver support system. The encapsulated hepatocytes remained viable and were able to synthesize protein for up to 3 weeks in culture. Allografts of encapsulated hepatocytes replaced the function of a damaged liver and reduced the mortality rate among rats with galactosamine-induced fulminant hepatic failure.