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1.
Environ Microbiol ; 22(6): 2150-2164, 2020 06.
Article in English | MEDLINE | ID: mdl-32141148

ABSTRACT

A clone encoding carboxymethyl cellulase activity was isolated during functional screening of a human gut metagenomic library using Lactococcus lactis MG1363 as heterologous host. The insert carried a glycoside hydrolase family 9 (GH9) catalytic domain with sequence similarity to a gene from Coprococcus eutactus ART55/1. Genome surveys indicated a limited distribution of GH9 domains among dominant human colonic anaerobes. Genomes of C. eutactus-related strains harboured two GH9-encoding and four GH5-encoding genes, but the strains did not appear to degrade cellulose. Instead, they grew well on ß-glucans and one of the strains also grew on galactomannan, galactan, glucomannan and starch. Coprococcus comes and Coprococcus catus strains did not harbour GH9 genes and were not able to grow on ß-glucans. Gene expression and proteomic analysis of C. eutactus ART55/1 grown on cellobiose, ß-glucan and lichenan revealed similar changes in expression in comparison to glucose. On ß-glucan and lichenan only, one of the four GH5 genes was strongly upregulated. Growth on glucomannan led to a transcriptional response of many genes, in particular a strong upregulation of glycoside hydrolases involved in mannan degradation. Thus, ß-glucans are a major growth substrate for species related to C. eutactus, with glucomannan and galactans alternative substrates for some strains.


Subject(s)
Clostridiales/growth & development , Gastrointestinal Microbiome , beta-Glucans , Bacterial Proteins/genetics , Clostridiales/genetics , Gene Expression , Glucans/pharmacology , Glycoside Hydrolases/genetics , Humans , Proteomics
2.
BMC Genomics ; 15: 1054, 2014 Dec 03.
Article in English | MEDLINE | ID: mdl-25467604

ABSTRACT

BACKGROUND: Lactococcus lactis is the most used species in the dairy industry. Its ability to adapt to technological stresses, such as oxidative stress encountered during stirring in the first stages of the cheese-making process, is a key factor to measure its technological performance. This study aimed to understand the response to oxidative stress of Lactococcus lactis subsp. cremoris MG1363 at the transcriptional and metabolic levels in relation to acidification kinetics and growth conditions, especially at an early stage of growth. For those purposes, conditions of hyper-oxygenation were initially fixed for the fermentation. RESULTS: Kinetics of growth and acidification were not affected by the presence of oxygen, indicating a high resistance to oxygen of the L. lactis MG1363 strain. Its resistance was explained by an efficient consumption of oxygen within the first 4 hours of culture, leading to a drop of the redox potential. The efficient consumption of oxygen by the L. lactis MG1363 strain was supported by a coherent and early adaptation to oxygen after 1 hour of culture at both gene expression and metabolic levels. In oxygen metabolism, the over-expression of all the genes of the nrd (ribonucleotide reductases) operon or fhu (ferrichrome ABC transports) genes was particularly significant. In carbon metabolism, the presence of oxygen led to an early shift at the gene level in the pyruvate pathway towards the acetate/2,3-butanediol pathway confirmed by the kinetics of metabolite production. Finally, the MG1363 strain was no longer able to consume oxygen in the stationary growth phase, leading to a drastic loss of culturability as a consequence of cumulative stresses and the absence of gene adaptation at this stage. CONCLUSIONS: Combining metabolic and transcriptomic profiling, together with oxygen consumption kinetics, yielded new insights into the whole genome adaptation of L. lactis to initial oxidative stress. An early and transitional adaptation to oxidative stress was revealed for L. lactis subsp. cremoris MG1363 in the presence of initially high levels of oxygen. This enables the cells to maintain key traits that are of great importance for industry, such as rapid acidification and reduction of the redox potential of the growth media.


Subject(s)
Adaptation, Physiological/genetics , Lactococcus lactis/genetics , Oxidative Stress/genetics , Transcriptome/genetics , Animals , Cattle , Fermentation/genetics , Food Microbiology , Lactococcus lactis/growth & development , Metabolomics , Milk/metabolism , Milk/microbiology , Oxidation-Reduction , Oxygen/metabolism , Phenotype
3.
Microbiology (Reading) ; 158(Pt 12): 2936-2945, 2012 Dec.
Article in English | MEDLINE | ID: mdl-23023974

ABSTRACT

The widely used plasmid-free Lactococcus lactis strain MG1363 was derived from the industrial dairy starter strain NCDO712. This strain carries a 55.39 kb plasmid encoding genes for lactose catabolism and a serine proteinase involved in casein degradation. We report the DNA sequencing and annotation of pLP712, which revealed additional metabolic genes, including peptidase F, d-lactate dehydrogenase and α-keto acid dehydrogenase (E3 complex). Comparison of pLP712 with other large lactococcal lactose and/or proteinase plasmids from L. lactis subsp. cremoris SK11 (pSK11L, pSK11P) and the plant strain L. lactis NCDO1867 (pGdh442) revealed their close relationship. The plasmid appears to have evolved through a series of genetic events as a composite of pGdh442, pSK11L and pSK11P. We describe in detail a scenario by which the metabolic genes relevant to the growth of its host in a milk environment have been unified on one replicon, reflecting the evolution of L. lactis as it changed its biological niche from plants to dairy environments. The extensive structural instability of pLP712 allows easy isolation of derivative plasmids lacking genes for casein degradation and/or lactose catabolism. Plasmid pLP712 is transferable by transduction and conjugation, and both of these processes result in significant molecular rearrangements. We report the detailed molecular analysis of insertion sequence element-mediated genetic rearrangements within pLP712 and several different mechanisms, including homologous recombination and adjacent deletion. Analysis of the integration of the lactose operon into the chromosome highlights the fluidity of the MG1363 integration hotspot and the potential for frequent movement of genes between plasmids and chromosomes in Lactococcus.


Subject(s)
DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Genomic Instability , Metabolic Networks and Pathways/genetics , Plasmids , Conjugation, Genetic , Evolution, Molecular , Gene Transfer, Horizontal , Industrial Microbiology , Lactococcus lactis/genetics , Lactococcus lactis/isolation & purification , Molecular Sequence Data , Recombination, Genetic , Sequence Analysis, DNA , Sequence Homology , Transformation, Genetic
4.
Appl Environ Microbiol ; 77(19): 6826-35, 2011 Oct.
Article in English | MEDLINE | ID: mdl-21841021

ABSTRACT

Manipulation of NADH-dependent steps, and particularly disruption of the las-located lactate dehydrogenase (ldh) gene in Lactococcus lactis, is common to engineering strategies envisaging the accumulation of reduced end products other than lactate. Reverse transcription-PCR experiments revealed that three out of the four genes assigned to lactate dehydrogenase in the genome of L. lactis, i.e., the ldh, ldhB, and ldhX genes, were expressed in the parental strain MG1363. Given that genetic redundancy is often a major cause of metabolic instability in engineered strains, we set out to develop a genetically stable lactococcal host tuned for the production of reduced compounds. Therefore, the ldhB and ldhX genes were sequentially deleted in L. lactis FI10089, a strain with a deletion of the ldh gene. The single, double, and triple mutants, FI10089, FI10089ΔldhB, and FI10089ΔldhBΔldhX, showed similar growth profiles and displayed mixed-acid fermentation, ethanol being the main reduced end product. Hence, the alcohol dehydrogenase-encoding gene, the adhE gene, was inactivated in FI10089, but the resulting strain reverted to homolactic fermentation due to induction of the ldhB gene. The three lactate dehydrogenase-deficient mutants were selected as a background for the production of mannitol and 2,3-butanediol. Pathways for the biosynthesis of these compounds were overexpressed under the control of a nisin promoter, and the constructs were analyzed with respect to growth parameters and product yields under anaerobiosis. Glucose was efficiently channeled to mannitol (maximal yield, 42%) or to 2,3-butanediol (maximal yield, 67%). The theoretical yield for 2,3-butanediol was achieved. We show that FI10089ΔldhB is a valuable basis for engineering strategies aiming at the production of reduced compounds.


Subject(s)
Butylene Glycols/metabolism , Lactococcus lactis/genetics , Lactococcus lactis/metabolism , Mannitol/metabolism , Metabolic Engineering , Metabolic Networks and Pathways/genetics , NAD/metabolism , Ethanol/metabolism , Fermentation , Gene Deletion , Gene Expression , Gene Expression Profiling , Glucose/metabolism , L-Lactate Dehydrogenase/genetics , L-Lactate Dehydrogenase/metabolism , Lactococcus lactis/growth & development , Promoter Regions, Genetic , Reverse Transcriptase Polymerase Chain Reaction
5.
Appl Environ Microbiol ; 76(9): 3026-31, 2010 May.
Article in English | MEDLINE | ID: mdl-20228099

ABSTRACT

Overexpression of the lactococcal CsiA protein affects the cell wall integrity of growing cells and leads to leakage of intracellular material. This property was optimized and exploited for the targeted release of biologically active compounds into the extracellular environment, thereby providing a new delivery system for bacterial proteins and peptides. The effects of different levels of CsiA expression on the leakage of endogenous lactate dehydrogenase and nucleic acids were measured and related to the impact of CsiA expression on Lactococcus lactis cell viability and growth. A leakage phenotype was obtained from cells expressing both recombinant and nonrecombinant forms of CsiA. As proof of principle, we demonstrated that CsiA promotes the efficient release of the heterologous Listeria bacteriophage endolysin LM4 in its active form. Under optimized conditions, native and heterologous active-molecule release is possible without affecting cell viability. The ability of CsiA to release intracellular material by controlled lysis without the requirement for an external lytic agent provides a technology for the control of both the extent of lysis and its timing. Taken together, these results demonstrate the potential of this novel approach for applications including product recovery in industrial fermentations, food processing, and medical therapy.


Subject(s)
Bacterial Proteins/metabolism , Lactococcus lactis/metabolism , Cell Wall/metabolism , Endopeptidases/metabolism , Lactococcus lactis/genetics
6.
J Bacteriol ; 191(22): 7142-3, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19767436

ABSTRACT

Lactobacillus johnsonii is a member of the acidophilus group of lactobacilli. Because of their probiotic properties, including attachment to epithelial cells, immunomodulation, and competitive exclusion of pathogens, representatives of this group are being intensively studied. Here we report the complete annotated genome sequence of Lactobacillus johnsonii FI9785, a strain which prevents the colonization of specific-pathogen-free chicks by Clostridium perfringens.


Subject(s)
Genome, Bacterial/genetics , Lactobacillus acidophilus/genetics , Poultry/microbiology , Animals , Molecular Sequence Data , Sequence Analysis, DNA
7.
Mol Microbiol ; 72(3): 779-94, 2009 May.
Article in English | MEDLINE | ID: mdl-19400771

ABSTRACT

Conjugation is a widely spread mechanism allowing bacteria to adapt and evolve by acquiring foreign DNA. The chromosome of Lactococcus lactis MG 1363 contains a 60 kb conjugative element called the sex factor capable of high-frequency DNA transfer. Yet, little is known about the proteins involved in this process. Comparative genomics revealed a close relationship between the sex factor and elements found in Gram-positive pathogenic cocci. Among the conserved gene products, CsiA is a large protein that contains a highly conserved domain (HCD) and a C-terminal cysteine, histidine-dependent amidohydrolases/peptidases (CHAP) domain in its C-terminal moiety. Here, we show that CsiA is required for DNA transfer. Surprisingly, increased expression of CsiA affects cell viability and the cells become susceptible to lysis. Point mutagenesis of HCD reveals that this domain is responsible for the observed phenotypes. Growth studies and electron microscope observations suggest that CsiA is acting as a cell wall synthesis inhibitor. In vitro experiments reveal the capacity of CsiA to bind d-Ala-d-Ala analogues and to prevent the action of penicillin binding proteins. Our results strongly suggest that CsiA sequesters the peptidoglycan precursor and prevents the final stage of cell wall biosynthesis to enable the localized assembly of the DNA transfer machinery through the cell wall.


Subject(s)
Bacterial Proteins/metabolism , Cell Wall/metabolism , Conjugation, Genetic , F Factor/metabolism , Lactococcus lactis/genetics , Bacterial Proteins/genetics , Carboxypeptidases/antagonists & inhibitors , Comparative Genomic Hybridization , Conserved Sequence , DNA, Bacterial/genetics , F Factor/genetics , Microbial Viability , Microscopy, Electron, Transmission , Multigene Family , Mutagenesis , Peptidoglycan/metabolism , Point Mutation
8.
Curr Microbiol ; 57(3): 200-5, 2008 Sep.
Article in English | MEDLINE | ID: mdl-18600375

ABSTRACT

E. coli fumarate nitrate reductase (FNR) binds to conserved FNR sites to regulate transcription under anaerobic condition. L. lactis subsp. cremoris MG1363 strain contains two FNR-like proteins (FlpA and B) encoded by flpA and flpB genes and the rcfA gene-encoded RcfA in L. lactis subsp. lactis IL1403 strain. Potential FNR-binding sites were located upstream of these genes. The flpA promoter is expressed in MG1363 anaerobically and aerobically. The flpB and rcfA promoters have typical class II FNR-dependent promoters and are activated anaerobically in MG1363 and IL1403, respectively. Despite their strong homology, the Flp and RcfA proteins cannot substitute for each other and control these promoters in the heterologous strains. The flpA and flpB promoters require FlpA and FlpB for activation in the MG1363 background. This was confirmed by expressing FlpB under nisin control in flp mutants and monitoring flpA promoter expression. In flpB- backgrounds, both FlpA and FlpB were required for flpA promoter expression. FlpB could not complement for the lack of FlpA protein in flpA- backgrounds.


Subject(s)
Bacterial Proteins/biosynthesis , Lactococcus lactis/metabolism , Bacterial Proteins/genetics , Gene Expression Regulation, Bacterial , Lactococcus lactis/genetics , Promoter Regions, Genetic , Succinate Dehydrogenase/genetics
9.
FEBS J ; 274(22): 5924-36, 2007 Nov.
Article in English | MEDLINE | ID: mdl-17944947

ABSTRACT

Lactococcus lactis FI9078, a construct carrying a disruption of the ldh gene, converted approximately 90% of glucose into lactic acid, like the parental strain MG1363. This unexpected lactate dehydrogenase activity was purified, and ldhB was identified as the gene encoding this protein. The activation of ldhB was explained by the insertion of an IS905-like element that created a hybrid promoter in the intergenic region upstream of ldhB. The biochemical and kinetic properties of this alternative lactate dehydrogenase (LDHB) were compared to those of the ldh-encoded enzyme (LDH), purified from the parental strain. In contrast to LDH, the affinity of LDHB for NADH and the activation constant for fructose 1,6-bisphosphate were strongly dependent on pH. The activation constant increased 700-fold, whereas the K(m) for NADH increased more than 10-fold, in the pH range 5.5-7.2. The two enzymes also exhibited different pH profiles for maximal activity. Moreover, inorganic phosphate acted as a strong activator of LDHB. The impact of replacing LDH by LDHB on the physiology of L. lactis was assessed by monitoring the evolution of the pools of glycolytic intermediates and cofactors during the metabolism of glucose by in vivo NMR. Structural analysis by comparative modeling of the two proteins showed that LDH has a slightly larger negative charge than LDHB and a greater concentration of positive charges at the interface between monomers. The calculated pH titration curves of the catalytic histidine residues explain why LDH maintains its activity at low pH as compared to LDHB, the histidines in LDH showing larger pH titration ranges.


Subject(s)
Genes, Bacterial , Isoenzymes/genetics , L-Lactate Dehydrogenase/genetics , Lactococcus lactis/genetics , Base Sequence , Catalysis , DNA Primers , Glucose/metabolism , Isoenzymes/chemistry , Isoenzymes/metabolism , Kinetics , L-Lactate Dehydrogenase/chemistry , L-Lactate Dehydrogenase/metabolism , Models, Molecular , Molecular Sequence Data
10.
Appl Environ Microbiol ; 73(23): 7771-80, 2007 Dec.
Article in English | MEDLINE | ID: mdl-17933937

ABSTRACT

In this study, we describe the genetic organizations of six and five apparent prophage-like elements present in the genomes of the Lactococcus lactis subsp. cremoris strains MG1363 and SK11, respectively. Phylogenetic investigation as well bioinformatic analyses indicates that all 11 prophages belong to subdivisions of the lactococcal P335 group of temperate bacteriophages.


Subject(s)
Genome, Bacterial , Lactococcus lactis/genetics , Prophages/genetics , Bacteriophages/classification , Bacteriophages/genetics , Chromosome Mapping , Computational Biology , Gene Expression Profiling , Open Reading Frames/genetics , Phylogeny , Species Specificity
11.
J Bacteriol ; 189(8): 3256-70, 2007 Apr.
Article in English | MEDLINE | ID: mdl-17307855

ABSTRACT

Lactococcus lactis is of great importance for the nutrition of hundreds of millions of people worldwide. This paper describes the genome sequence of Lactococcus lactis subsp. cremoris MG1363, the lactococcal strain most intensively studied throughout the world. The 2,529,478-bp genome contains 81 pseudogenes and encodes 2,436 proteins. Of the 530 unique proteins, 47 belong to the COG (clusters of orthologous groups) functional category "carbohydrate metabolism and transport," by far the largest category of novel proteins in comparison with L. lactis subsp. lactis IL1403. Nearly one-fifth of the 71 insertion elements are concentrated in a specific 56-kb region. This integration hot-spot region carries genes that are typically associated with lactococcal plasmids and a repeat sequence specifically found on plasmids and in the "lateral gene transfer hot spot" in the genome of Streptococcus thermophilus. Although the parent of L. lactis MG1363 was used to demonstrate lysogeny in Lactococcus, L. lactis MG1363 carries four remnant/satellite phages and two apparently complete prophages. The availability of the L. lactis MG1363 genome sequence will reinforce its status as the prototype among lactic acid bacteria through facilitation of further applied and fundamental research.


Subject(s)
Genome, Bacterial , Lactococcus lactis/genetics , Bacillus Phages/genetics , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Base Sequence , Carbohydrate Metabolism , Genome, Viral , Lactococcus lactis/metabolism , Lactococcus lactis/virology , Molecular Sequence Data , Plasmids/genetics , Prophages/genetics
12.
J Bacteriol ; 188(6): 2106-14, 2006 Mar.
Article in English | MEDLINE | ID: mdl-16513740

ABSTRACT

CluA is a cell surface-presented protein that causes cell aggregation and is essential for a high-efficiency conjugation process in Lactococcus lactis. We know from previous work that in addition to promoting cell-to-cell contact, CluA is involved in sex factor DNA transfer. To define the CluA domains involved in aggregation and in transfer, we first performed random mutagenesis of the cluA gene using a modified mini-Tn7 element which generated five amino acid insertions located throughout the encoded protein. Thirty independent cluA insertion mutants expressing modified CluA proteins at the cell surface were isolated and characterized further. The level of aggregation of each mutant was determined. The cell binding capacity of CluA was affected strongly when the protein had a mutation in its N-terminal region, which defined an aggregation domain extending from amino acid 153 to amino acid 483. Of the cluA mutants that still exhibited aggregation, eight showed an attenuated ability to conjugate, and six mutations were located in a 300-amino-acid C-terminal region of the protein defining a transfer domain (Tra). This result was confirmed by a phenotypic analysis of an additional five mutants obtained using site-directed mutagenesis in which charged amino acids of the Tra domain were replaced by alanine residues. Two distinct functional domains of the CluA protein were defined in this work; the first domain is involved in cell binding specificity, and the Tra domain is probably involved in the formation of the DNA transport machinery. This is the first report of a protein involved in conjugation that actively contributes to DNA transfer and mediates contact between donor and recipient strains.


Subject(s)
Bacterial Proteins/physiology , Conjugation, Genetic/physiology , DNA, Bacterial/metabolism , F Factor/metabolism , Lactococcus lactis/physiology , Membrane Proteins/physiology , Amino Acid Sequence , Amino Acid Substitution , Bacterial Adhesion , Bacterial Proteins/genetics , Conjugation, Genetic/genetics , DNA Transposable Elements , Lactococcus lactis/genetics , Membrane Proteins/genetics , Molecular Sequence Data , Mutagenesis, Insertional , Mutagenesis, Site-Directed , Mutation, Missense , Protein Structure, Tertiary/genetics , Protein Structure, Tertiary/physiology , Recombination, Genetic
13.
Mol Microbiol ; 57(4): 1101-12, 2005 Aug.
Article in English | MEDLINE | ID: mdl-16091047

ABSTRACT

Dps proteins play a major role in the protection of bacterial DNA from damage by reactive oxygen species. Previous studies have implicated the extended lysine-containing N-terminal regions of Dps subunits in DNA binding, but this part of the structure has not previously been observed crystallographically. Here the structures of two Dps proteins (DpsA and DpsB) from Lactococcus lactis MG1363 reveal for the first time the presence of an N-terminal alpha helix that extends from the core of the Dps subunit. Consequently, the N-terminal helices are displayed in parallel pairs on the exterior of the dodecameric Dps assemblies. Both DpsA and DpsB bind DNA. Deletion of the DpsA N-terminal helix impaired DNA binding. The N-terminal Lys residues of Escherichia coli Dps have been implicated in DNA binding. Replacement of the lactococcal DpsA Lys residues 9, 15 and 16 by Glu did not inhibit DNA binding. However, DNA binding was inhibited by EDTA, suggesting a role for cations in DNA binding. In contrast to E. coli, Bacillus brevis and Mycobacterium smegmatis Dps:DNA complexes, in which DNA interacts with crystalline Dps phases, L. lactis DNA:Dps complexes appeared as non-crystalline aggregates of protein and DNA in electron micrographs.


Subject(s)
Bacterial Proteins/chemistry , DNA-Binding Proteins/chemistry , Lactococcus lactis/metabolism , Amino Acid Sequence , Bacterial Proteins/metabolism , Bacterial Proteins/ultrastructure , Crystallography, X-Ray , DNA/chemistry , DNA/metabolism , DNA/ultrastructure , DNA-Binding Proteins/metabolism , DNA-Binding Proteins/ultrastructure , Molecular Sequence Data , Open Reading Frames , Protein Structure, Secondary , Solutions/chemistry
14.
Microbiology (Reading) ; 150(Pt 8): 2503-2512, 2004 Aug.
Article in English | MEDLINE | ID: mdl-15289547

ABSTRACT

CluA is a 136 kDa surface-bound protein encoded by the chromosomally located sex factor of Lactococcus lactis MG1363 and is associated with cell aggregation linked to high-frequency transfer of the sex factor. To further investigate the involvement of CluA in these phenomena, the cluA gene was cloned on a plasmid, downstream from the lactococcal nisA promoter. In a sex-factor-negative MG1363 derivative, nisin-controlled CluA expression resulted in aggregation, despite the absence of the other genes of the sex factor. Therefore, CluA is the only sex factor component responsible for aggregation. The direct involvement of CluA in the establishment of cell-to-cell contact for aggregate formation was observed by electron microscopy using immunogold-labelled CluA antibodies. Inactivation of cluA in an MG1363 background led to a dramatic decrease in sex factor conjugation frequency compared to the parental strain. Increasing levels of CluA expressed in trans in the cluA-inactivated donor strain facilitated a gradual restoration of conjugation frequency, reaching that of the parental strain. In conclusion, CluA is essential for efficient sex factor transfer in conjugation of L. lactis.


Subject(s)
Bacterial Proteins/genetics , Conjugation, Genetic , Lactococcus lactis/genetics , Animals , Antibodies, Bacterial , Bacterial Proteins/immunology , Bacterial Proteins/isolation & purification , DNA, Bacterial/genetics , F Factor/genetics , Gene Expression Regulation, Bacterial , Immunohistochemistry , Lac Operon , Lactococcus lactis/growth & development , Lactococcus lactis/ultrastructure , Microscopy, Electron , Nisin/genetics , Promoter Regions, Genetic
15.
Appl Environ Microbiol ; 70(3): 1466-74, 2004 Mar.
Article in English | MEDLINE | ID: mdl-15006767

ABSTRACT

Mannitol is a sugar polyol claimed to have health-promoting properties. A mannitol-producing strain of Lactococcus lactis was obtained by disruption of two genes of the phosphoenolpyruvate (PEP)-mannitol phosphotransferase system (PTS(Mtl)). Genes mtlA and mtlF were independently deleted by double-crossover recombination in strain L. lactis FI9630 (a food-grade lactate dehydrogenase-deficient strain derived from MG1363), yielding two mutant (Delta ldh Delta mtlA and Delta ldh Delta mtlF) strains. The new strains, FI10091 and FI10089, respectively, do not possess any selection marker and are suitable for use in the food industry. The metabolism of glucose in nongrowing cell suspensions of the mutant strains was characterized by in vivo (13)C-nuclear magnetic resonance. The intermediate metabolite, mannitol-1-phosphate, accumulated intracellularly to high levels (up to 76 mM). Mannitol was a major end product, one-third of glucose being converted to this hexitol. The double mutants, in contrast to the parent strain, were unable to utilize mannitol even after glucose depletion, showing that mannitol was taken up exclusively by PEP-PTS(Mtl). Disruption of this system completely blocked mannitol transport in L. lactis, as intended. In addition to mannitol, approximately equimolar amounts of ethanol, 2,3-butanediol, and lactate were produced. A mixed-acid fermentation (formate, ethanol, and acetate) was also observed during growth under controlled conditions of pH and temperature, but mannitol production was low. The reasons for the alteration in the pattern of end products under nongrowing and growing conditions are discussed, and strategies to improve mannitol production during growth are proposed.


Subject(s)
Lactococcus lactis/genetics , Lactococcus lactis/metabolism , Mannitol/metabolism , Base Sequence , Biological Transport, Active , DNA, Bacterial/genetics , Escherichia coli Proteins , Food Microbiology , Genes, Bacterial , Genetic Engineering , Glucose/metabolism , L-Lactate Dehydrogenase/genetics , L-Lactate Dehydrogenase/metabolism , Lactococcus lactis/growth & development , Magnetic Resonance Spectroscopy , Models, Biological , Monosaccharide Transport Proteins , Mutation , Operon , Phosphoenolpyruvate Sugar Phosphotransferase System/genetics , Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism , Plasmids/genetics , Recombination, Genetic , Sugar Alcohol Dehydrogenases/genetics , Sugar Alcohol Dehydrogenases/metabolism
16.
Microbiology (Reading) ; 148(Pt 11): 3467-3476, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12427938

ABSTRACT

Mannitol metabolism in Lactococcus lactis MG1363 and in a derivative strain deficient in lactate dehydrogenase (LDH(d)) was characterized. Both strains had the ability to grow on mannitol as an energy source, although this polyol was a poorer substrate for growth than glucose. When compared to glucose, the metabolism of mannitol caused an NADH burden due to formation of an additional NADH molecule at the reaction catalysed by mannitol-1-phosphate dehydrogenase (Mtl1PDH). This resulted in a prominent accumulation of mannitol 1-phosphate (Mtl1P) both in growing and resting cells, suggesting the existence of a severe bottleneck at Mtl1PDH. Growth on mannitol induced the activity of Mtl1PDH in both the LDH(d) and MG1363 strains. The lower accumulation of Mtl1P in mannitol-grown cells when compared to glucose-grown LDH(d) cells, as monitored by in vivo (13)C-NMR, reflects this induction. A clear shift towards the production of ethanol was observed on mannitol, indicating pressure to regenerate NAD(+) when this substrate was used. A strategy to obtain a mannitol-overproducing strain is proposed.


Subject(s)
L-Lactate Dehydrogenase/metabolism , Lactococcus lactis/metabolism , Mannitol/metabolism , Biomass , Carbon Isotopes , Fructose/metabolism , Glucose/metabolism , Glyceric Acids/metabolism , L-Lactate Dehydrogenase/deficiency , L-Lactate Dehydrogenase/genetics , Lactococcus lactis/enzymology , Lactococcus lactis/genetics , Magnetic Resonance Spectroscopy , Mutation
17.
J Biol Chem ; 277(31): 28088-98, 2002 Aug 02.
Article in English | MEDLINE | ID: mdl-12011086

ABSTRACT

The involvement of nicotinamide adenine nucleotides (NAD(+), NADH) in the regulation of glycolysis in Lactococcus lactis was investigated by using (13)C and (31)P NMR to monitor in vivo the kinetics of the pools of NAD(+), NADH, ATP, inorganic phosphate (P(i)), glycolytic intermediates, and end products derived from a pulse of glucose. Nicotinic acid specifically labeled on carbon 5 was synthesized and used in the growth medium as a precursor of pyridine nucleotides to allow for in vivo detection of (13)C-labeled NAD(+) and NADH. The capacity of L. lactis MG1363 to regenerate NAD(+) was manipulated either by turning on NADH oxidase activity or by knocking out the gene encoding lactate dehydrogenase (LDH). An LDH(-) deficient strain was constructed by double crossover. Upon supply of glucose, NAD(+) was constant and maximal (approximately 5 mm) in the parent strain (MG1363) but decreased abruptly in the LDH(-) strain both under aerobic and anaerobic conditions. NADH in MG1363 was always below the detection limit as long as glucose was available. The rate of glucose consumption under anaerobic conditions was 7-fold lower in the LDH(-) strain and NADH reached high levels (2.5 mm), reflecting severe limitation in regenerating NAD(+). However, under aerobic conditions the glycolytic flux was nearly as high as in MG1363 despite the accumulation of NADH up to 1.5 mm. Glyceraldehyde-3-phosphate dehydrogenase was able to support a high flux even in the presence of NADH concentrations much higher than those of the parent strain. We interpret the data as showing that the glycolytic flux in wild type L. lactis is not primarily controlled at the level of glyceraldehyde-3-phosphate dehydrogenase by NADH. The ATP/ADP/P(i) content could play an important role.


Subject(s)
Glycolysis/physiology , Lactococcus lactis/metabolism , NAD/metabolism , Aerobiosis , Carbon Isotopes , Crossing Over, Genetic , DNA Primers , Glucose/metabolism , Homeostasis , L-Lactate Dehydrogenase/metabolism , Lactococcus lactis/genetics , Lactococcus lactis/growth & development , Magnetic Resonance Spectroscopy , Models, Biological , Multienzyme Complexes/metabolism , NADH, NADPH Oxidoreductases/metabolism , Niacin/metabolism , Oxidation-Reduction
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