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1.
Plant Physiol ; 192(2): 1221-1233, 2023 05 31.
Article in English | MEDLINE | ID: mdl-36703219

ABSTRACT

The chloroplast ATP synthase produces the ATP needed for photosynthesis and plant growth. The trans-membrane flow of protons through the ATP synthase rotates an oligomeric assembly of c subunits, the c-ring. The ion-to-ATP ratio in rotary F1F0-ATP synthases is defined by the number of c-subunits in the rotor c-ring. Engineering the c-ring stoichiometry is, therefore, a possible route to manipulate ATP synthesis by the ATP synthase and hence photosynthetic efficiency in plants. Here, we describe the construction of a tobacco (Nicotiana tabacum) chloroplast atpH (chloroplastic ATP synthase subunit c gene) mutant in which the c-ring stoichiometry was increased from 14 to 15 c-subunits. Although the abundance of the ATP synthase was decreased to 25% of wild-type (WT) levels, the mutant lines grew as well as WT plants and photosynthetic electron transport remained unaffected. To synthesize the necessary ATP for growth, we found that the contribution of the membrane potential to the proton motive force was enhanced to ensure a higher proton flux via the c15-ring without unwanted low pH-induced feedback inhibition of electron transport. Our work opens avenues to manipulate plant ion-to-ATP ratios with potentially beneficial consequences for photosynthesis.


Subject(s)
Nicotiana , Photosynthesis , Nicotiana/genetics , Nicotiana/metabolism , Chloroplasts/metabolism , Electron Transport , Chloroplast Proton-Translocating ATPases/genetics , Chloroplast Proton-Translocating ATPases/metabolism , Protons , Adenosine Triphosphate/metabolism
2.
Methods Mol Biol ; 1305: 281-99, 2015.
Article in English | MEDLINE | ID: mdl-25910742

ABSTRACT

The alternative oxidase (AOX) is an integral monotopic membrane protein located on the inner surface of the inner mitochondrial membrane. Branching from the traditional respiratory chain at the quinone pool, AOX is responsible for cyanide-resistant respiration in plants and fungi, heat generation in thermogenic plants, and survival of parasites, such as Trypanosoma brucei, in the human host. A recently solved AOX structure provides insight into its active site, thereby facilitating rational phytopathogenic and antiparasitic drug design. Here, we describe expression of recombinant AOX using two different expression systems. Purification protocols for the production of highly pure and stable AOX protein in sufficient quantities to facilitate further kinetic, biophysical, and structural analyses are also described.


Subject(s)
Magnoliopsida/enzymology , Magnoliopsida/genetics , Mitochondrial Proteins/chemistry , Mitochondrial Proteins/genetics , Oxidoreductases/chemistry , Oxidoreductases/genetics , Plant Proteins/chemistry , Plant Proteins/genetics , Chromatography, Gel/methods , Cloning, Molecular/methods , Crystallization/methods , Enzyme Assays/methods , Escherichia coli/genetics , Magnoliopsida/chemistry , Magnoliopsida/metabolism , Mitochondrial Proteins/isolation & purification , Mitochondrial Proteins/metabolism , Oxidoreductases/isolation & purification , Oxidoreductases/metabolism , Plant Proteins/isolation & purification , Plant Proteins/metabolism , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolism , Schizosaccharomyces/genetics , Solubility , Transformation, Genetic
3.
Mitochondrion ; 19 Pt B: 261-8, 2014 Nov.
Article in English | MEDLINE | ID: mdl-24632469

ABSTRACT

The alternative oxidase (AOX) is a non-protonmotive ubiquinol oxidase that is found in mitochondria of all higher plants studied to date. Structural and functional characterisation of this important but enigmatic plant diiron protein has been hampered by an inability to obtain sufficient native protein from plant sources. In the present study recombinant SgAOX (rSgAOX), overexpressed in a ΔhemA-deficient Escherichia coli strain (FN102), was solubilized from E. coli membranes and purified to homogeneity in a stable and highly active form. The kinetics of ubiquinol-1 oxidation by purified rSgAOX showed typical Michaelis-Menten kinetics (K(m) of 332 µM and Vmax of 30 µmol(-1) min(-1) mg(-1)), a turnover number 20 µmol s(-1) and a remarkable stability. The enzyme was potently inhibited not only by conventional inhibitors such as SHAM and n-propyl gallate but also by the potent TAO inhibitors ascofuranone, an ascofuranone-derivative colletochlorin B and the cytochrome bc1 inhibitor ascochlorin. Circular dichroism studies revealed that AOX was approximately 50% α-helical and furthermore such studies revealed that rSgAOX and rTAO partially retained the helical absorbance signal even at 90 °C (58% and 64% respectively) indicating a high conformational stability. It is anticipated that highly purified and active AOX and its mutants will facilitate investigations into the structure and reaction mechanisms of AOXs through the provision of large amounts of purified protein for crystallography and contribute to further progress of the study on this important plant terminal oxidase.


Subject(s)
Araceae/enzymology , Mitochondrial Proteins/metabolism , Oxidoreductases/metabolism , Plant Proteins/metabolism , Araceae/genetics , Circular Dichroism , Cloning, Molecular , Enzyme Inhibitors/analysis , Enzyme Stability , Escherichia coli/genetics , Escherichia coli/metabolism , Gene Expression , Hot Temperature , Kinetics , Mitochondrial Proteins/chemistry , Mitochondrial Proteins/genetics , Mitochondrial Proteins/isolation & purification , Oxidation-Reduction , Oxidoreductases/chemistry , Oxidoreductases/genetics , Oxidoreductases/isolation & purification , Plant Proteins/chemistry , Plant Proteins/genetics , Plant Proteins/isolation & purification , Protein Conformation , Protein Stability , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolism , Ubiquinone/analogs & derivatives , Ubiquinone/metabolism
4.
Biochim Biophys Acta ; 1837(7): 1219-25, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24530866

ABSTRACT

In the present paper we have investigated the effect of mutagenesis of a number of highly conserved residues (R159, D163, L177 and L267) which we have recently shown to line the hydrophobic inhibitor/substrate cavity in the alternative oxidases (AOXs). Measurements of respiratory activity in rSgAOX expressed in Escherichia coli FN102 membranes indicate that all mutants result in a decrease in maximum activity of AOX and in some cases (D163 and L177) a decrease in the apparent Km (O2). Of particular importance was the finding that when the L177 and L267 residues, which appear to cause a bottleneck in the hydrophobic cavity, are mutated to alanine the sensitivity to AOX antagonists is reduced. When non-AOX anti-malarial inhibitors were also tested against these mutants widening the bottleneck through removal of isobutyl side chain allowed access of these bulkier inhibitors to the active-site and resulted in inhibition. Results are discussed in terms of how these mutations have altered the way in which the AOX's catalytic cycle is controlled and since maximum activity is decreased we predict that such mutations result in an increase in the steady state level of at least one O2-derived AOX intermediate. Such mutations should therefore prove to be useful in future stopped-flow and electron paramagnetic resonance experiments in attempts to understand the catalytic cycle of the alternative oxidase which may prove to be important in future rational drug design to treat diseases such as trypanosomiasis. Furthermore since single amino acid mutations in inhibitor/substrate pockets have been found to be the cause of multi-drug resistant strains of malaria, the decrease in sensitivity to main AOX antagonists observed in the L-mutants studied in this report suggests that an emergence of drug resistance to trypanosomiasis may also be possible. Therefore we suggest that the design of future AOX inhibitors should have structures that are less reliant on the orientation by the two-leucine residues. This article is part of a Special Issue entitled: 18th European Bioenergetic Conference.


Subject(s)
Araceae/enzymology , Mitochondrial Proteins/metabolism , Mutation, Missense , Oxidoreductases/metabolism , Plant Proteins/metabolism , Ubiquinone/analogs & derivatives , Amino Acid Sequence , Binding Sites , Cell Membrane/metabolism , Escherichia coli/genetics , Escherichia coli/metabolism , Mitochondrial Proteins/antagonists & inhibitors , Mitochondrial Proteins/chemistry , Mitochondrial Proteins/genetics , Molecular Sequence Data , Mutagenesis, Site-Directed , Oxidoreductases/antagonists & inhibitors , Oxidoreductases/chemistry , Oxidoreductases/genetics , Plant Proteins/antagonists & inhibitors , Plant Proteins/chemistry , Plant Proteins/genetics , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Terpenes/pharmacology , Ubiquinone/metabolism
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