ABSTRACT
A method is described for routine quantitation of very low numbers of white cells (WBCs) in platelet components, using flow cytometry and dual nucleic acid stains. The WBC quantitation is based on the detection of nucleated cells labeled with propidium iodide and thiazole orange, with chicken red cells added as an internal counting standard. Platelet concentrates containing 0.001 to 1500 WBCs per microL (2 x 10(2)-3 x 10(8) WBCs/component) and 600 to 2800 x 10(3) platelets per microL were analyzed for the number of contaminating WBCs. The method was found to be linear throughout the 7 log10 range and to have sufficient reproducibility and sensitivity for routine analysis of WBC-reduced platelet concentrates.
Subject(s)
Blood Platelets/cytology , Leukocyte Count , DNA/blood , Erythrocyte Count/methods , Flow Cytometry/methods , Fluorescence , Humans , Leukocyte Count/methods , Light , RNA/blood , Reproducibility of Results , Scattering, RadiationABSTRACT
This report describes a new system for collection of platelet concentrate (PC) and cell-free plasma (PPP) from apheresis donors. The system uses two separation devices and requires only a single venipuncture. The Plateletcell device separates primary platelet concentrate (PPC) from anticoagulated whole blood and the Plasmacell-C device separates the PPC into PC and PPP. Results of functional studies performed indicate that the separation process does not alter viability of either the PPC, the PC, or the PPP. Platelet function after 5 days of storage is maintained. An average yield of 3.4 +/- 0.7 x 10(11) platelets in 201 g of PC and 422 g of PPP were harvested in 71 +/- 13 min of donor time from donors with preprocedure hematocrits averaging 42.5 +/- 2.0% and preprocedure platelet counts averaging 265 +/- 61 x 10(3)/microliters.
