ABSTRACT
Transverse colon interposition for oesophageal replacement following oesophagectomy is a rarely seen entity; interposition of the colon exterior to the thoracic cage is rarer still. Here, we report the case of a patient presenting with a large bowel obstruction in a transverse colon interposition located subcutaneously anterior to the sternum, with the diaphragm fully intact, following chest compressions for multiple cardiac arrests. The need for specialty input was highlighted early on, and the large bowel decompressed via nasogastric tube insertion into the interpositioned colon. The patient was then transferred to the acute coronary unit for further observation and management.
Subject(s)
Intestinal Obstruction , Humans , Intestinal Obstruction/diagnostic imaging , Intestinal Obstruction/etiology , Intestinal Obstruction/surgery , Colon/diagnostic imaging , Colon/surgery , Esophagus/surgery , Diaphragm , Esophagectomy/adverse effectsABSTRACT
A polypeptide antigen of 74.0 kDa molecular weight was detected in the antigenic extracts of the 3 developmental stages of Schistosoma mansoni (eggs, cercariae, and adult worms) by western blotting using BRL4 monoclonal antibody (mAb) that significantly protected mice at the levels of 51.6%, 42%, and 53.8% against challenge S. mansoni infection in 3 separate experiments. This antigen was isolated and purified from crude soluble worm antigen preparation by immunoaffinity chromatography using CNBr-activated sepharose-4B beads coupled with the BRL4 mAb. The purified antigen showed a single peak when analyzed by both high-performance liquid chromatography and high-performance capillary electrophoresis. The 74-kDa antigen was characterized as a protein in nature with 56.9% hydrophilic amino acids and 43.1% hydrophobic amino acids. This antigen was detected in 93% of urine samples from infected cases with specificity of 89% among noninfected cases using an enzyme immunoassay-fast dot-enzyme-linked immunosorbent assay based on BRL4 mAb.
Subject(s)
Antigens, Helminth/isolation & purification , Schistosoma mansoni/immunology , Adolescent , Adult , Aged , Amino Acids/analysis , Animals , Antibodies, Helminth/immunology , Antibodies, Monoclonal/immunology , Antigens, Helminth/chemistry , Antigens, Helminth/urine , Blotting, Western , Child , Child, Preschool , Chromatography, Affinity , Chromatography, High Pressure Liquid , Electrophoresis, Polyacrylamide Gel , Enzyme-Linked Immunosorbent Assay , Female , Humans , Hybridomas , Immunization, Passive , Mice , Mice, Inbred BALB C , Middle Aged , Peptide Mapping , Schistosomiasis mansoni/immunology , Schistosomiasis mansoni/urineABSTRACT
A study of the absorptive function of the small intestine in chidlhood malignant lymphomas was carried out at the Cancer Institute of Cairo University. The study comprised twenty-five children, with stage III or IV lymphoma. A complete lymphoma work-up was performed for each patient, comprising clinical, pathological, haematological, biochemical and roentgenological assessments. Evaluation of small intestinal function was done by means of mucosal biopsy, small bowel motor meal, xylose excretion test, and estimation of faecal fat excretion. These tests revealed evidence of malabsorption in 69% of the patients. The study provides a better understanding of childhood malignant lymphomas in Egypt, and shows that malabsorption may be more common in the disease than has been previously suspected.