ABSTRACT
Function of islets of Langerhans of Wistar male rats was studied after irradiation of animals with different doses. The pancreas function was determined with regard to the incorporation of 65Zn into the gland tissues. Total-body irradiation (60Co) with the dose of 4.0 Gy somewhat increased the function of islets; at the dose of 8.0 Gy their function was transiently blocked, and at the dose of 15 Gy the function of islets of Langerhans was blocked completely.
Subject(s)
Islets of Langerhans/physiopathology , Radiation Injuries, Experimental/physiopathology , Animals , Cobalt Radioisotopes , Gamma Rays , Male , Rats , Rats, Inbred Strains , Whole-Body Irradiation , Zinc RadioisotopesABSTRACT
For investigation of postirradiation reactions, of pancreatic insular cells, male Wistar rats were subjected to 1.5, 3, 7, and 15 kR of Co60 gamma-radiation. It was found that after application of doses of 7 kR or higher, interphase death of all the insular A-cells occurred within 6 hours. The cell death was demonstrated to be characterized by chromatin pycnosis, dilatation of the endoplasmic reticulum and perinuclear space, mitochondrial swelling, and desintegration of A-granules. Macrophages took no part in resorption of the dead cells. A-cell necrosis was not accompanied by morphologically observable changes in acinar cells or insucar B-cells. Some possible explanations of the more pronounced radiosensitivity of A-cells are discussed.
Subject(s)
Cobalt Radioisotopes/administration & dosage , Radiation Injuries, Experimental/pathology , Animals , Cell Survival/radiation effects , Islets of Langerhans/pathology , Islets of Langerhans/radiation effects , Islets of Langerhans/ultrastructure , Male , Microscopy, Electron , Radiation Dosage , Rats , Time FactorsABSTRACT
In Wistar rats a study was made of a functional condition of the thyroid gland 1, 6 and 24 hours after partial pancreatectomy. The following signs-served as criteria of a functional condition of the gland: the amount of neutrone-activated iodine in iron, iodine-absorptive capacity of the gland by I131, the amount of protein-bound stable and radioactive iodine in the blood, a morphological condition of the gland structures. It was demonstrated that, despite the initial intensification of the process of synthesis and secretion in the thyroid gland, its secretory activity fell by the end of the experiment. This reduction is regarded as an adaptive response to partial insulin deficiency.
