ABSTRACT
Proteins (--SS, --SH, and NH2 groups; tyrosine, tryptophan and arginine), DNA, phospholipids and triglycerides showed a progressive decline in the oocytes of Locusta migratoria with increase in doses of apholate, tepa and hempa. Carbohydrates showed a decline only after higher doses of apholate and tepa whereas they were not affected at all with hempa. The pyroninophilia due to RNA in the oocytes decreased in the ooplasm of oocytes slightly, but increased in the cytoplasm and nucleoplasm of follicular epithelial cells with all the chemosterilants. The follicular epithelial cells degenerated in the mature oocytes, and yolk formation was inhibited.
Subject(s)
Aziridines/pharmacology , Azirines/pharmacology , Grasshoppers/drug effects , Hempa/pharmacology , Oocytes/metabolism , Organophosphorus Compounds/pharmacology , Ovum/metabolism , Animals , Carbohydrate Metabolism , DNA/metabolism , Female , Grasshoppers/physiology , Lipid Metabolism , Proteins/metabolism , Triethylenephosphoramide/pharmacologyABSTRACT
5-Fluorouracil caused necrosis in ovary only whereas testis remained unaffected even with higher doses. In ovary the ooplasm shrank; some follicular epithelial cells became pycnotic, and the nuclei of others showed an abnormal fragmentation of chromatin material; the cytoplasm of follicular epithelial cells was drawn into the peripheral empty space formed by the contraction of the ooplasm, and ultimately the follicular epithelium disintegrated. The interoocytal bridge is some oocytes was lost. The yolk formation was inhibited, and maturation of eggs was prevented. Most of the mature oocytes were also damaged. Inhibition of DNA and RNA synthesis, and decreases in proteins, carbohydrates and lipids was observed. With increased doses and post-treatment periods the effects mentioned above were increased.
Subject(s)
Fluorouracil/pharmacology , Grasshoppers/anatomy & histology , Ovary/cytology , Testis/cytology , Animals , Dose-Response Relationship, Drug , Female , Histocytochemistry , MaleABSTRACT
Columnar epithelium in Suncus murinus is secretory and the secretory product is rich in proteins and polysaccharides; it is devoid of lipids. The secretion is discharged into the lumen by a process of blebbing from the acinar tips of the columnar cells. The basal cells only replace the worn-out columnar cells.
