ABSTRACT
The intracellular fluorescein fluorescence polarization (IFFP) test indicates that peripheral blood lymphocytes (PBL) of cancer patients display stimulatory sensitivity to a short incubation with specific tumor protein extracts. In this work, a human lymphocyte activation melanoma antigen (LAMA) was purified from supernatant of a human melanoma cell line (L1M1), which could specifically stimulate lymphocytes of melanoma patients. The results showed a significant stimulation of lymphocytes from healthy donors after incubation with phytohaemagglutinin (PHA), while no stimulation was observed after incubation with LAMA. On the other hand, lymphocytes from melanoma patients showed a significant stimulation with LAMA, while generally showing minor or no stimulation with PHA. Melanoma specificity of LAMA was demonstrated by no response in lymphocytes from patients of lung, colon, or breast cancer. The purified fraction is therefore considered to be a shared tissue-specific antigen which may be useful in immunodiagnosis and immunotherapy of melanoma.
Subject(s)
Antigens, Neoplasm , Lymphocyte Activation , Lymphocytes/immunology , Melanoma/diagnosis , Neoplasm Proteins , Antigens, Neoplasm/immunology , Antigens, Neoplasm/isolation & purification , Biomarkers, Tumor , Chromatography, DEAE-Cellulose , Electrophoresis, Polyacrylamide Gel , Fluorescein , Fluorescence Polarization , Humans , Melanoma/chemistry , Melanoma/immunology , Melanoma-Specific Antigens , Neoplasm Proteins/immunology , Neoplasm Proteins/isolation & purification , Phytohemagglutinins , Tumor Cells, CulturedSubject(s)
Bulimia/physiopathology , Buspirone , Disruptive, Impulse Control, and Conduct Disorders/physiopathology , Serotonin Receptor Agonists , Adrenocorticotropic Hormone/blood , Adult , Bulimia/diagnosis , Buspirone/pharmacokinetics , Disruptive, Impulse Control, and Conduct Disorders/diagnosis , Double-Blind Method , Female , Growth Hormone/blood , Humans , Hydrocortisone/blood , Personality Inventory , Prolactin/blood , Receptors, Serotonin/physiology , Serotonin Receptor Agonists/pharmacokineticsABSTRACT
The effect of two anthracyclines--doxorubicin hydrochloride (adriamycin, CAS-25316-40-9), and 4'-epidoxorubicin (epirubicin, CAS-56420-45-2), and an anthracenedione, mitoxantrone (CAS-70476-82-3) on the element content of cultured embryonic rat cardiomyocytes was examined by X-ray microanalysis. Adriamycin induced an increase in Ca, P and S, and a decrease in Cl content. Novantrone caused a marked increase in the Na and a decrease in the Cl content, whereas epirubicin did not affect the content of any of the elements examined. In an attempt to prevent the effect of the drugs on the element content of the cells, the cardiomyocytes were incubated with a blocker of the slow Ca channels--verapamil-- and with each of the three drugs. Verapamil only induced an increase in the Ca and a decrease in the S content of the cells. Except for some protective effect on the element content of the cardiomyocytes, which was altered by adriamycin, the results did not indicate any beneficial effect of verapamil added to the other two drugs, as for this aspect of the study.
