[Comparative characterization of the polystyrene plates used in enzyme immunoassay]. / Sravnitel'naia kharakteristika polistirolovykh planshetov, primeniaemykh v IFA.

The data on the comparison of the quality of polystyrene plates manufactured in the USSR with those manufactured by a number of foreign producers are presented. These data indicate that the capacity of antibodies adsorbed on a polystyrene plate for the sorption of antigens depend on the properties of the polymer surface and vary for different plates.

[Possibilities of using immunoenzyme test systems for determining levels of myoglobin, fibrinogen, fibrin-fibrinogen degradation products and fibronectin in the diagnosis of somatic diseases]. / Vozmozhnosti primeneniia immunofermentnykh test-sistem dlia opredeleniia soderzhaniia mioglobina, fibrinogena i produktov degradatsii fibrin-fibrinogena, fibronektina v diagnostike somaticheskikh zabolevanii.

Clinical trials of immunoenzyme test-systems for the quantitative determination of fibronectin, fibrinogen, fibrin/fibrinogen degradation products and myoglobin have been performed on serum and plasma samples obtained from patients and healthy donors. The tests were informative and possessed diagnostic value in the following conditions: fibronectin--in pyogenic septic complications of newborns, burn infections; fibrinogen and fibrin/fibrinogen products--in thrombosis, myocardial infarction and disseminated intravascular coagulation syndrome; myoglobin--in myocardial infarction.

[Isolation, characterization, and translation of heterogeneous nuclear RNA from plasmacytoma cells]. / Vydelenie, kharakteristika i transliatsiia geterogennoi iadernoi RNK iz kletok plazmotsitomy.

It was found that during hot phenol fractionation of plasmacytoma MOPC-21 cells, which produce immunoglobulins of gamma 1 chi-type, separation of nuclear and nucleolar RNAs, differing in kinetics of accumulation of radioactive precursors, distribution during gradient centrifugation procedures and in base composition, takes place. D-RNA 63 degrees and D-RNA 85 degrees, which possess similar characteristics with D-RNAs, discovered earlier in other cells were isolated. It was stated, the D-RNA 85 degrees is considerably enriched by 3'-terminal poly(A) sequences in comparison with D-RNA 63 degrees. During translation in cell-free protein synthesising system from Xenopus oocytes, these two fractions of hnRNA induced formation of immunospecific products. D-RNA 85 degrees stimulated synthesis of gamma 1 and chi chains more efficiently, that D-RNA 63 degrees. Polyadenylated and non-adenylated both D-RNAs stimulated synthesis gamma 1 and chi chains of immunoglobulin G1 during translation. This fact indicates the absence of significant differences in the informational content. These results give opportunity to propose the existence of two levels of processing pre-mRNA for immunoglobulin chains.