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1.
Thorax ; 57(6): 524-7, 2002 Jun.
Article in English | MEDLINE | ID: mdl-12037228

ABSTRACT

BACKGROUND: Smooth muscle contraction is one of the hallmarks of asthma. A recently developed pyridine derivative, Y-27632, a selective Rho kinase inhibitor, has been reported to inhibit the smooth muscle contraction of human and animal trachea in ex vivo systems but its effect in animal models of airway hyperresponsiveness (AHR) has not been examined. The purpose of this study was to evaluate the effect of Y-27632 in a murine model of allergic and virally induced AHR. METHODS: Baseline lung resistance and methacholine induced AHR were measured in mice sensitised to ovalbumin (OVA) and also in mice infected with respiratory syncytial virus (RSV) following ovalbumin sensitisation (OVA/RSV). RESULTS: Time course and dose ranging experiments indicated that 30 mg/kg Y-27632 given by gavage 2 hours before methacholine challenge significantly reduced baseline lung resistance and prevented AHR in OVA sensitised mice. Y-27632 also suppressed AHR induced by the bronchospastic agent serotonin in OVA sensitised mice and prevented methacholine induced AHR in OVA/RSV mice. CONCLUSIONS: These results suggest that the signalling pathway mediated through Rho kinase may have an important role in bronchial smooth muscle tone in allergen induced and virus induced AHR and should be considered as a novel target for asthma treatment.


Subject(s)
Antihypertensive Agents/therapeutic use , Benzopyrans/therapeutic use , Bronchial Hyperreactivity/drug therapy , Respiratory Syncytial Virus Infections/complications , Airway Resistance/drug effects , Animals , Asthma/drug therapy , Asthma/physiopathology , Bronchial Hyperreactivity/etiology , Dose-Response Relationship, Drug , Female , Lung/drug effects , Mice , Mice, Inbred BALB C , Ovalbumin
2.
J Infect Dis ; 184(11): 1374-9, 2001 Dec 01.
Article in English | MEDLINE | ID: mdl-11709778

ABSTRACT

Severe respiratory syncytial virus (RSV) infection has been hypothesized to be a risk factor for the development of allergy and asthma, but epidemiologic studies in humans have been inconclusive. By use of a well-characterized murine model of RSV infection and allergic sensitization with ovalbumin, the effect of a preceding severe RSV infection on the development of the pulmonary allergic inflammatory response and airway hyperresponsiveness (AHR) was tested. The impact of prior allergic sensitization on RSV-induced illness, as measured by weight loss, also was evaluated. RSV infection before allergic sensitization decreased allergen-induced AHR, production of interleukin-13 in lung tissue, and lung eosinophilia. In contrast, allergic sensitization before RSV infection increased AHR and decreased RSV-related weight loss and lung levels of interferon-gamma but did not alter viral clearance. These data provide evidence that RSV-associated AHR occurs in hosts with allergic responses and that allergic inflammation is diminished when preceded by RSV infection.


Subject(s)
Hypersensitivity/immunology , Ovalbumin/immunology , Respiratory Syncytial Virus Infections/complications , Allergens/immunology , Animals , Bronchial Hyperreactivity/diagnosis , Bronchial Hyperreactivity/virology , Bronchoconstrictor Agents , Female , Hypersensitivity/complications , Interferon-gamma/biosynthesis , Interleukin-13/biosynthesis , Lung/immunology , Lung/virology , Lymphocyte Count , Methacholine Chloride , Mice , Mice, Inbred BALB C , Pulmonary Eosinophilia/complications , Pulmonary Eosinophilia/immunology , Respiratory Syncytial Virus Infections/immunology , Respiratory Syncytial Virus Infections/virology , Respiratory Syncytial Viruses/growth & development , Time Factors , Virus Replication , Weight Loss
3.
Immunol Res ; 23(2-3): 179-91, 2001.
Article in English | MEDLINE | ID: mdl-11444383

ABSTRACT

The central goal of our laboratory is to understand the regulation of lymphoid cells through molecular mechanisms of signal transduction and transcriptional control. A long-standing focus has been on changes that influence the effector function of mature lymphocytes. Work in the laboratory is oriented toward the identification of new regulatory mechanisms using cell lines and primary cells, and the validation of these in vitro findings in mouse models of immune responses and diseases. In this review, we summarize key insights into the regulation of T helper cell function during the phase of immunity where effector responses arise de novo. Particular interest has been centered on cytokine gene regulation as part of T cell differentiation into the Th1 and Th2 subsets. Information on IL-4 receptor signaling and the role of NF-kappaB transcription factors is reviewed. Our more recent work is designed to understand how regulation at the Th1/2 effector stages is related to the control of memory T cell survival, immune recall responses, and the role of these responses in immune-mediated disease.


Subject(s)
Gene Expression Regulation/physiology , Interleukin-4/physiology , Signal Transduction/physiology , T-Lymphocyte Subsets/immunology , Transcription, Genetic/physiology , Adoptive Transfer , Animals , Asthma/immunology , Chromatin/genetics , Chromatin/ultrastructure , Cytokines/biosynthesis , Cytokines/genetics , Cytokines/physiology , Humans , I-kappa B Proteins/physiology , Immunologic Memory , Mice , Mice, Inbred BALB C , Mice, Knockout , Mice, Transgenic , NF-kappa B/physiology , Protein Kinases/physiology , Proto-Oncogene Proteins c-bcl-2/physiology , Receptors, Interleukin-4/physiology , T-Lymphocytes, Cytotoxic/immunology , Th1 Cells/immunology , Th2 Cells/immunology , Transcription Factors/physiology
4.
Clin Exp Allergy ; 31(3): 387-90, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11260149

ABSTRACT

Asthma is a chronic inflammatory disease of the airways which may involve an oxidant injury to the lung. Assessment of oxidant stress is difficult in vivo, but measurement of F2-isoprostanes (F2-IsoPs), free radical-catalysed products of arachidonic acid, appears to offer a reliable approach for quantitative measurement of oxidative stress status in vivo. We have recently developed a mass spectrometric assay for 2,3-dinor-5,6-dihydro-15-F2t-IsoP (15-F2t-IsoP-M), the major urinary metabolite of the F2-IsoP, 15-F2t-IsoP (8-iso-PGF2a). Measurement of the urinary excretion of this metabolite offers a reliable index of oxidative stress status in vivo that has advantages over measuring unmetabolized F2-IsoPs in urine and plasma. To assess the occurrence of oxidative stress in patients with atopic asthma following allergen exposure in vivo by measuring the urinary excretion of 15-F2t-IsoP-M. Analysis of 15-F2t-IsoP-M by GC-NICI-MS in nine mild atopic asthmatics following inhaled allergen provocation and four asthmatic subjects after inhaled challenge with methacholine. Urinary excretion of 15-F2t-IsoP-M increased at 2 h after allergen challenge and remained significantly elevated in all urine collections during the subsequent 8-h period of the study compared to the baseline value (ANOVA, and Student-Newman-Keuls multiple comparisons test). No increase in the urinary excretion of 15-F2t-IsoP-M occurred after inhalation of methacholine. Allergen challenge causes an oxidant injury in human atopic asthmatics. 15-F2t-IsoP-M is a valuable marker of oxidant stress in vivo.


Subject(s)
Asthma/urine , Dinoprost/urine , Oxidative Stress , Allergens , Asthma/immunology , Dinoprost/analogs & derivatives , F2-Isoprostanes , Humans , Hypersensitivity/immunology , Hypersensitivity/urine
5.
J Med Virol ; 63(2): 178-88, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11170055

ABSTRACT

Severe respiratory syncytial virus (RSV)-induced disease is associated with childhood asthma and atopy. We combined murine models of allergen-sensitization and RSV infection to explore the interaction of allergic and virus-induced airway inflammation and its impact on airway hyperresponsiveness (AHR). We found that RSV infection during ova-sensitization (OVA/RSV) increased and prolonged AHR compared to mice only RSV-infected (RSV) or ova-sensitized (OVA). AHR is known to be associated with an increase in Type 2 cytokines (IL-4, IL-5, and IL-13) in allergen-sensitized mice. Therefore, we hypothesized that RSV-induced enhancement of AHR was a result of potentiating the Type 2 cytokine profile promoted by ova-sensitization. Surprisingly, we found that Type 2 cytokines induced by ova-sensitization were not increased by RSV infection despite the increase in AHR, and in some cases were diminished. RNAse protection assay revealed no difference in IL-4 and IL-5 mRNA levels between the OVA and OVA/RSV groups, and IL-13 mRNA was significantly decreased in the OVA/RSV mice compared to the OVA group. Flow cytometric analysis of Type 2 cytokines demonstrated the same frequency of IL-4 and IL-5 production in lung-derived T lymphocytes from the OVA/RSV and OVA groups. Direct cytokine ELISA measurements of lung supernatant showed the level of IL-13 was significantly decreased in the OVA/RSV group compared to OVA mice, while there was no difference in either IL-4 or IL-5 between these two groups. These data indicate that the enhanced and prolonged AHR caused by the interaction of allergic airway inflammation and virus-induced immune responses is a complex process that can not be explained simply by augmented production of Type 2 cytokines.


Subject(s)
Allergens/immunology , Hypersensitivity/immunology , Respiratory Syncytial Virus Infections/immunology , Respiratory Syncytial Viruses , Animals , Cytokines/analysis , Cytokines/metabolism , Disease Models, Animal , Female , Flow Cytometry , Immunization , Interleukin-13/metabolism , Interleukin-4/metabolism , Interleukin-5/metabolism , Lung/immunology , Lymphocytes/immunology , Mice , Mice, Inbred BALB C , Ovalbumin/immunology , RNA, Messenger/analysis , Specific Pathogen-Free Organisms
6.
Methods Mol Med ; 56: 411-22, 2001.
Article in English | MEDLINE | ID: mdl-21336918

ABSTRACT

Metabolism of arachidonic acid results in a host of biologically active compounds with profound effects on airway inflammation (1). After activation of cellular phospholipases and release of free arachidonic acid, catalyzed insertion of oxygen occurs enzymatically via action of one of the two known cyclooxygenase isoenzymes (COX-1 and COX-2). The unstable bicyclic intermediate, PGH(2), undergoes subsequent metabolism to form prostaglandins (PG), thromboxane (Tx), and leukotrienes (LT) (see Fig.1). In addition, free radicals can oxygenate arachidonate although it is bound to the diacylgycerol backbone of membrane phospholipids. The family of compounds formed in this way, known as isoprostanes, are stereochemically different and incorporate a large number of regioisomeric compounds that may confound measurement of PG (2-5 and see Chapter 33 ). Arachidonic acid can also be metabolized by specific cytochrome P(450) enzymes to regioisomeric epoxides and stereo specific hydro xyeicosatetraenoic (HETE) acids (6). Fig. 1. Overview of pathways of metabolism of arachidonic acid during airway inflammation. Following activation of cellular phospholipases, arachidonic acid is cleaved from membrane phospholipids. It is undergoing dioxygenation catalyzed by cyclooxygenase (either COX-1 or COX-2 isoforms) to form the unstable endoperoxide intermediate PGH2. Specific isomerases with varied cellular distribution further metabolize PGH2 to bioactive prostaglandins and thromboxanes.

7.
Methods Mol Med ; 56: 423-30, 2001.
Article in English | MEDLINE | ID: mdl-21336919

ABSTRACT

The discoveries by Jack Roberts and Jason Morrow of the nonenzymatic oxidation of cell membrane phospholipids to form isoprostanes has revolutionized the field of eicosanoids (1). Prior to their discoveries, it was dogma that the important biologically active eicosanoids were formed by enzymes acting on arachidonic acid that had been cleaved from phospholipids by the action of phospholipases. Their research has clearly shown that important biologically active fatty acid metabolites are formed in a variety of inflammatory conditions from the action of oxygen radicals on arachidonic acid, while it is still present in complex phospholipids. These oxidized compounds may alter cell structure and signaling, and when released by the action of phospholipases, are immediately available to bind to receptors to modulate cell activity. The free radical attack on arachidonate yields an endoperoxide which can then be transformed nonenzymatically to F, D, E ring prostaglandins. Thus, each enzymatically formed eicosanoid appears to have its own class of isoprostanes, including isothromboxanes (2,3). Likewise, the isoleukotrienes have been described. In addition, compounds such as the hydroxyeicosatetraenoic acids (HETEs) can also be formed in this fashion (4,5). The biologic activity of these compounds is only now being examined.

8.
Am J Respir Crit Care Med ; 162(3 Pt 1): 878-82, 2000 Sep.
Article in English | MEDLINE | ID: mdl-10988099

ABSTRACT

Eosinophilic bronchitis is a common cause of chronic cough, which like asthma is characterized by sputum eosinophilia, but in contrast to asthma there is no variable airflow obstruction or airway hyperresponsiveness. Our hypothesis was that the differences in airway pathophysiology maybe due to less active airway inflammation in eosinophilic bronchitis, with reduced release of important effector mediators. We measured the concentration of various proinflammatory mediators in induced sputum cell-free supernatant in eight patients with eosinophilic bronchitis, 17 patients with asthma matched for sputum eosinophil count, and 10 normal subjects. Cysteinyl-leukotrienes (cys-LT) were measured by enzyme immunoassay, eosinophilic cationic protein (ECP) by fluoroimmunoassay, prostanoids (PGE(2), PGD(2), TXB(2), and PGF(2alpha)) by gas chromatography-negative ion chemical ionization-mass spectroscopy, and histamine by radioenzymic assay. The geometric mean sputum eosinophil count was similar in asthma (13.4%) and eosinophilic bronchitis (12.5%). Sputum cys-LT and ECP were a mean (95% CI) 1.6-fold (1.1, 2.5) and 6.4-fold (1.4, 28) higher in eosinophilic bronchitis and 1.9-fold (1.3, 2.9) and 7.7-fold (1.2, 46) higher in asthma compared with that in control subjects (geometric mean, 5.9 and 95 ng/ml, respectively). In eosinophilic bronchitis the mean concentration of sputum PGD(2) (0.79 ng/ml) and histamine (168 ng/ml) were significantly higher than in asthma (mean absolute difference in PGD(2) concentration, 0.47 ng/ml [95% CI, 0.19 to 0. 74] and mean-fold difference in histamine concentration, 6.7 [95% CI 1.7 to 26]) and normal subjects (0.64 ng/ml [0.36 to 0.90] and 11-fold [3.3 to 36]), respectively. In conclusion, eosinophilic bronchitis is associated with active airway inflammation with increased release of vasoactive and bronchoconstrictor mediators.


Subject(s)
Asthma/immunology , Bronchitis/immunology , Eosinophilia/immunology , Inflammation Mediators/metabolism , Sputum/immunology , Adult , Aged , Asthma/diagnosis , Bronchitis/diagnosis , Eosinophilia/diagnosis , Eosinophils/immunology , Female , Humans , Leukocyte Count , Male , Middle Aged
9.
J Infect Dis ; 182(3): 671-7, 2000 Sep.
Article in English | MEDLINE | ID: mdl-10950758

ABSTRACT

Respiratory syncytial virus (RSV)-induced disease is associated with recurrent episodes of wheezing in children, and an effective vaccine currently is not available. The use of 2 immunizations (a formalin-inactivated, alum-precipitated RSV vaccine [FI-RSV] given intramuscularly and live RSV given intranasally [LVIN]), with a control immunization, were compared in a well-characterized model of RSV challenge, with or without concomitant allergic sensitization with ovalbumin. FI-RSV caused a significant increase in airway hyperresponsiveness in mice after RSV infection during allergic sensitization, and this was associated with an increase in type 2 cytokine production. In contrast, immunization with LVIN did not change type 2 cytokine production and protected against RSV-induced airway hyperresponsiveness in the setting of allergic sensitization. This study suggests that immune modulation with RSV vaccination can have profound effects on RSV-induced airway disease and that prevention of airway hyperresponsiveness is an important end point in vaccine development.


Subject(s)
Respiratory Hypersensitivity/immunology , Respiratory Syncytial Viruses/immunology , Vaccines, Inactivated/immunology , Viral Vaccines/immunology , Administration, Intranasal , Animals , Disease Models, Animal , Female , Formaldehyde , Methacholine Chloride , Mice , Mice, Inbred BALB C , Respiratory Hypersensitivity/chemically induced , Respiratory Hypersensitivity/virology , Respiratory Syncytial Virus Infections/immunology , Respiratory Syncytial Viruses/drug effects , Vaccines, Inactivated/administration & dosage , Viral Vaccines/administration & dosage
10.
Am J Respir Crit Care Med ; 162(2 Pt 1): 637-40, 2000 Aug.
Article in English | MEDLINE | ID: mdl-10934099

ABSTRACT

Prostaglandin E(2) (PGE(2)) inhibits the early and late bronchoconstrictor response to inhaled allergen. The mechanisms of action, however, are not understood. We investigated the effect of inhaled PGE(2) on the release of prostaglandin D(2) (PGD(2)), preformed mast cell mediators, and other products of arachidonic acid metabolism. We compared inhaled PGE(2) (100 microgram) to placebo in a randomized double-blind crossover study. Ten atopic asthmatics underwent bronchoscopy immediately after inhalation of PGE(2) or placebo. Bronchoalveolar lavage (BAL) was performed at baseline, and in a separate segment 4 min after allergen instillation. Nebulized PGE(2) was well tolerated. PGE(2) concentrations in baseline lavage fluid were significantly greater after PGE(2) inhalation than after placebo. PGD(2) concentrations after allergen challenge were significantly reduced in those subjects receiving nebulized PGE(2) compared with control subjects. We conclude that PGE(2) can be safely delivered by inhalation. Nebulized PGE(2) administered before to segmental allergen challenge reduced PGD(2) in BAL fluid (BALF). PGE(2) also decreased the production of other mediators of the arachidonic acid pathway, although not significantly. The reduction of PGD(2) may be part of the mechanism by which PGE(2) blocks the early asthmatic response.


Subject(s)
Asthma/drug therapy , Dinoprostone/pharmacology , Prostaglandin D2/metabolism , Administration, Inhalation , Adolescent , Adult , Allergens , Asthma/immunology , Bronchoalveolar Lavage Fluid/chemistry , Bronchoscopy , Cross-Over Studies , Dinoprostone/administration & dosage , Double-Blind Method , Humans , Lung/metabolism , Prostaglandin D2/analysis
11.
Am J Respir Crit Care Med ; 162(2 Pt 1): 676-81, 2000 Aug.
Article in English | MEDLINE | ID: mdl-10934105

ABSTRACT

The immunomodulatory role of arachidonic acid metabolites in allergic sensitization is undefined. Prostaglandin E(2) (PGE(2)), a product of arachidonic acid metabolism through the cyclooxygenase pathway, has been reported to favor Type 2-like cytokine secretion profiles in murine and human CD4(+) T cells by inhibiting the production of Type 1-associated cytokines. On the basis of these in vitro data, we hypothesized that indomethacin, a nonselective cyclooxygenase inhibitor, would diminish allergen-induced production of Type 2 cytokines in mice, and protect against airway hyperresponsiveness (AHR) to methacholine. We found that ovalbumin-sensitized mice that were treated with indomethacin (OVA-indomethacin mice) had significantly greater AHR (p < 0.05) and higher levels of IL-5 (176 +/- 52 versus 66 +/- 4 pg/ml) and IL-13 (1,226 +/- 279 versus 475 +/- 65 pg/ml) in lung supernatants than mice sensitized with ovalbumin alone (OVA mice), while levels of IL-4 and serum IgE were not different. Lung mRNA expression of the C-C chemokine MCP-1 was increased in OVA-indomethacin mice, while there was no difference between the two groups in lung mRNA expression of eotaxin, MIP-1alpha, MIP-1beta, or MIP-2. Histologic examination revealed greater pulmonary interstitial eosinophilia in OVA-indomethacin mice as well. Contrary to our expectations, we conclude that in the BALB/c mouse, cyclooxygenase inhibition during allergen sensitization increases AHR, production of IL-5 and IL-13, and interstitial eosinophilia.


Subject(s)
Cyclooxygenase Inhibitors/pharmacology , Hypersensitivity/physiopathology , Indomethacin/pharmacology , Interleukin-13/biosynthesis , Interleukin-5/biosynthesis , Respiratory Hypersensitivity/metabolism , Animals , Arachidonic Acid/metabolism , Chemokines, CC/analysis , Female , Immunoglobulin E/blood , Interleukin-4/analysis , Interleukin-6/analysis , Lung/chemistry , Lung/pathology , Methacholine Chloride , Mice , Mice, Inbred BALB C , Ovalbumin/immunology , Respiratory Hypersensitivity/prevention & control , Specific Pathogen-Free Organisms
12.
J Appl Physiol (1985) ; 88(6): 2214-8, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10846038

ABSTRACT

PGE(2) is an important cyclooxygenase product that modulates airway inflammatory and smooth muscle responses. Signal transduction is mediated by four EP receptor subtypes that cause distinct effects on cell metabolism. To determine the role of EP(2) receptor activation, we produced a mouse lacking the EP(2) receptor by targeted gene disruption. The effect of aerosolized PGE(2) and other agonists was measured using barometric plethysmography and by measurements of lung resistance in mechanically ventilated mice. Inhalation of PGE(2) inhibited methacholine responses in wild-type but not in mice lacking the EP(2) receptor [EP(2)(-/-)]. After airway constriction was induced by methacholine aerosol, PGE(2) reduced the airway constriction enhanced pause in wild-type mice (from 0.88 +/- 0.15 to 0.55 +/- 0.06) but increased it in EP(2)(-/-) mice (from 0.73 +/- 0. 08 to 1.27 +/- 0.19). Similar results were obtained in mechanically ventilated mice. These data indicate that the EP(2) receptor mediates the bronchodilation effect of PGE(2).


Subject(s)
Bronchodilator Agents/pharmacology , Dinoprostone/pharmacology , Receptors, Prostaglandin E/physiology , Administration, Inhalation , Airway Resistance/drug effects , Animals , Bronchi/drug effects , Bronchoconstriction/drug effects , Bronchoconstrictor Agents/pharmacology , Lung/drug effects , Methacholine Chloride/pharmacology , Mice , Mice, Knockout/genetics , Receptors, Prostaglandin E/genetics , Receptors, Prostaglandin E, EP2 Subtype
13.
Am J Respir Crit Care Med ; 161(5): 1553-8, 2000 May.
Article in English | MEDLINE | ID: mdl-10806153

ABSTRACT

We have used the relatively noninvasive technique of induced sputum to measure allergen-induced changes in the concentration of eicosanoid mediators in bronchial secretions from atopic asthmatics. Sputum induction was performed before and 24 h after inhalational allergen challenge in 14 atopic asthmatics who developed a late asthmatic reaction (LAR). Differential cell counts were made on sputum cytospins and eicosanoid (cysteinyl leukotrienes [cys LTs], prostaglandin D(2) [PGD(2)], and PGE(2)) concentrations were measured in the sputum supernatants. The percentage of eosinophils at baseline correlated with the concentration of cys LTs (r = 0.84, p < 0.001) but not prostanoid mediators. Allergen challenge produced a significant increase in the concentration of sputum cys LTs from 3. 45 ng/ml sputum to 11.95 ng/ml (p = 0.002), which correlated with the increase in sputum eosinophils (r = 0.55, p < 0.05). There were no significant changes in PGD(2) or PGE(2) concentrations in sputum supernatants in response to challenge. Thus, the noninvasive technique of induced sputum has been used to demonstrate increased cys LTs, but not prostanoids associated with LAR after allergen challenge. The correlation between eosinophil numbers and cys LT concentrations at baseline values and 24 h after allergen challenge is consistent with these cells being a principal source of cys LTs within the airways at these time points.


Subject(s)
Allergens/administration & dosage , Asthma/metabolism , Hypersensitivity, Immediate/metabolism , Leukotrienes/analysis , Sputum/chemistry , Adult , Asthma/pathology , Bronchial Provocation Tests , Cell Count , Dinoprostone/analysis , Female , Humans , Hypersensitivity, Immediate/pathology , Leukotriene C4/analysis , Leukotriene D4/analysis , Leukotriene E4/analysis , Male , Middle Aged , Prostaglandin D2/analysis , Sputum/cytology
14.
Am J Respir Crit Care Med ; 160(6): 1905-9, 1999 Dec.
Article in English | MEDLINE | ID: mdl-10588604

ABSTRACT

Further definition of the role of leukotrienes (LT) and prostaglandins (PG) in asthma would be helped by a noninvasive method for assessing airway production. The supernatant from sputum induced with hypertonic saline and dispersed using dithiotrietol has been successfully used to measure other molecular markers of airway inflammation and might be a useful method. We have measured induced sputum supernatant LTC(4)/D(4)/E(4) concentrations using enzyme immunoassay and PGE(2), PGD(2), TXB(2), and PGF(2alpha) using gas chromatography-negative ion chemical ionization-mass spectroscopy in 10 normal subjects and in 26 subjects with asthma of variable severity. Sputum cysteinyl-leukotrienes concentrations were significantly greater in subjects with asthma (median, 9.5 ng/ml) than in normal control subjects (6.4 ng/ml; p < 0.02) and greater in subjects with persistent asthma requiring inhaled corticosteroids (median, 11.4 ng/ml) or studied within 48 h of an acute severe exacerbation of asthma (13 ng/ml) than in subjects with episodic asthma treated with inhaled beta(2)-agonists only (7.2 ng/ml). There were no significant differences in the concentrations of other eicosanoids between groups, although there was a negative correlation between the percentage sputum eosinophil count and sputum PGE(2) concentration (r = -0.48; p < 0.01) in subjects with asthma. We conclude that induced sputum contains high concentrations of eicosanoids and that sputum LTC(4)/D(4)/E(4) concentrations are significantly greater in subjects with asthma than in normal subjects. The inverse relationship between eosinophilic airway inflammation and sputum PGE(2) concentration would be consistent, with the latter having an anti-inflammatory role.


Subject(s)
Asthma/metabolism , Eicosanoids/analysis , Sputum/chemistry , Adult , Asthma/drug therapy , Asthma/pathology , Cysteine/analysis , Eosinophils , Female , Humans , Inflammation Mediators/analysis , Leukocyte Count , Leukotrienes/analysis , Male , Middle Aged , Prostaglandins/analysis , Sputum/cytology , Thromboxane B2/analysis
15.
Am J Respir Crit Care Med ; 160(6): 1947-51, 1999 Dec.
Article in English | MEDLINE | ID: mdl-10588611

ABSTRACT

It is thought that reactive oxygen species (ROS) participate in the inflammation which characterizes asthma, but the evidence supporting this contention is incomplete. F(2)-isoprostanes (F(2)-IsoPs) are arachidonate products formed on membrane phospholipids by the action of ROS and thereby represent a quantitative measure of oxidant stress in vivo. Using a mass spectrometric assay we measured urinary release of F(2)-IsoPs in 11 patients with mild atopic asthma after inhaled allergen challenge. The excretion of F(2)-IsoPs increased at 2 h after allergen (1.5 +/- 0.2 versus 2.6 +/- 0.3 ng/mg creatinine) and remained significantly elevated in all urine collections for the 8-h period of the study (analysis of variance [ANOVA]). The measured compounds were of noncyclooxygenase origin because neither aspirin nor indomethacin given before challenge suppressed them. Urinary F(2)-IsoPs remained unchanged after inhaled methacholine challenge. In nine atopic asthmatics, F(2)-IsoPs were quantified in bronchoalveolar lavage fluid (BALF) at baseline values and in a separate segment 24 h after allergen instillation. F(2)-IsoPs were elevated late in the BALF (0.9 +/- 0.2 versus 11.4 +/- 3.0 pg /ml, baseline versus allergen, respectively, p = 0.007). The increase was inhibited by pretreatment of the subjects with inhaled corticosteroids. These findings provide a new evidence for a role for ROS and lipid peroxidation in allergen-induced airway inflammation.


Subject(s)
Allergens , Asthma/metabolism , Bronchial Provocation Tests , Oxidative Stress , Prostaglandins F/biosynthesis , Administration, Inhalation , Adult , Asthma/drug therapy , Asthma/immunology , Asthma/physiopathology , Beclomethasone/administration & dosage , Bronchoalveolar Lavage Fluid/chemistry , Cross-Over Studies , Cyclooxygenase Inhibitors/pharmacology , Double-Blind Method , Forced Expiratory Volume , Glucocorticoids/administration & dosage , Humans , Hypersensitivity, Immediate/complications , Hypersensitivity, Immediate/metabolism , Mass Spectrometry , Methacholine Chloride , Middle Aged , Prostaglandins F/urine , Reactive Oxygen Species/physiology , Skin Tests
16.
J Immunol ; 163(9): 5116-24, 1999 Nov 01.
Article in English | MEDLINE | ID: mdl-10528218

ABSTRACT

T cell function is a critical determinant of immune responses as well as susceptibility to allergic diseases. Activated T cells can differentiate into effectors whose cytokine profile is limited to type 1 (IFN-gamma-dominant) or type 2 (IL-4-, IL-5-dominant) patterns. To investigate mechanisms that connect extracellular stimuli with the regulation of effector T cell function, we have measured immune responses of transgenic mice whose NF-kappa B/Rel signaling pathway is inhibited in T cells. Surprisingly, these mice developed type 2 T cell-dependent responses (IgE and eosinophil recruitment) in a model of allergic pulmonary inflammation. In contrast, type 1 T cell responses were severely impaired, as evidenced by markedly diminished delayed-type hypersensitivity responses, IFN-gamma production, and Ag-specific IgG2a levels. Taken together, these data indicate that inhibition of NF-kappa B can lead to preferential impairment of type 1 as compared with type 2 T cell-dependent responses.


Subject(s)
I-kappa B Proteins , NF-kappa B/physiology , Proto-Oncogene Proteins c-rel/physiology , Th1 Cells/immunology , Th2 Cells/immunology , Animals , Bronchial Hyperreactivity/genetics , Bronchial Hyperreactivity/immunology , Bronchial Hyperreactivity/pathology , Cell Movement/genetics , Cell Movement/immunology , Cytokines/biosynthesis , DNA-Binding Proteins/genetics , Eosinophilia/genetics , Eosinophilia/immunology , Eosinophilia/pathology , Hypersensitivity, Delayed/genetics , Hypersensitivity, Delayed/immunology , Immunoglobulin Isotypes/biosynthesis , Lung/immunology , Lung/pathology , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Inbred DBA , Mice, Transgenic , NF-KappaB Inhibitor alpha , NF-kappa B/antagonists & inhibitors , Proto-Oncogene Proteins c-rel/antagonists & inhibitors , Respiratory Hypersensitivity/genetics , Respiratory Hypersensitivity/immunology , Respiratory Hypersensitivity/pathology , Th1 Cells/metabolism , Th2 Cells/metabolism , Transcription Factor RelA
17.
Chest ; 115(2): 475-81, 1999 Feb.
Article in English | MEDLINE | ID: mdl-10027449

ABSTRACT

STUDY OBJECTIVES: The purpose of this cross-sectional study was to confirm the observation that pulse oximetry tracing correlates with pulsus paradoxus, and is therefore a measure of the severity of air trapping in obstructive airway disease. DESIGN: Cross-sectional survey. SETTING: The ICU in a tertiary care academic hospital. PATIENTS: Twenty-six patients consecutively admitted to the ICU with obstructive airway disease, either asthma or COPD. MEASUREMENTS AND RESULTS: Forty-six percent of the study patients required mechanical ventilation, and 69% had an elevated pulsus paradoxus. We defined the altered pulse oximetry baseline tracing as the respiratory waveform variation (RWV). The RWV was measured in numerical form as the change in millimeters from the baseline. Pulsus paradoxus was significantly correlated with the RWV of the pulse oximetry tracing (p < 0.0001). An analysis of the respiratory variations in the pulse oximetry waveforms in obstructive lung disease patients reflects the presence and degree of auto-positive end-expiratory pressure (auto-PEEP; p < 0.0001). CONCLUSIONS: We describe the characteristic alterations in the pulse oximetry tracings that occur in the presence of pulsus paradoxus and auto-PEEP. Since pulse oximetry is available universally in ICUs and emergency departments, it may be a useful noninvasive means of continually assessing pulsus paradoxus and air trapping severity in obstructive airway disease patients.


Subject(s)
Asthma/physiopathology , Lung Diseases, Obstructive/physiopathology , Oximetry , Respiratory Mechanics , Adult , Aged , Asthma/therapy , Cross-Sectional Studies , Humans , Lung Diseases, Obstructive/therapy , Middle Aged , Respiration, Artificial
18.
J Med Virol ; 57(2): 186-92, 1999 Feb.
Article in English | MEDLINE | ID: mdl-9892406

ABSTRACT

Severe respiratory syncytial virus (RSV)-induced disease is associated with childhood asthma and atopy. We combined models of allergen sensitization and RSV infection to begin exploring the immunologic interactions between allergic and virus-induced airway inflammation and its impact on airway hypersensitivity. Airway resistance was measured after methacholine challenge in tracheally intubated mice by whole body plethysmography. Lung inflammation was assessed by bronchoalveolar lavage (BAL) and histopathology. RSV infection alone did not cause significant airway hyperresponsiveness (AHR) to methacholine. Ovalbumin (OVA)-induced AHR lasted only a few days past the discontinuance of OVA aerosol in mice that were ovalbumin sensitized and mock infected. In contrast, OVA-sensitized mice infected with RSV during the OVA aerosol treatments (OVA/RSV) had AHR for more than 2 weeks after infection. However, 2 weeks after either RSV or mock infection, OVA/RSV mice had significantly more lymphocytes found during BAL than OVA mice, whereas the OVA and OVA/RSV groups had the same number of eosinophils. Histopathologic analysis confirmed an increased inflammation in the lungs of OVA/RSV mice compared with OVA mice. In addition, OVA/RSV mice had a more widespread distribution of mucus in their airways with increased amounts of intraluminal mucus pools compared with the other groups. Thus, prolonged AHR in RSV-infected mice during ovalbumin-sensitization correlates with increased numbers of lymphocytes in BAL fluid, increased lung inflammation, and mucus deposition in the airways, but not with airway eosinophilia. A further understanding of the immunologic consequences of combined allergic and virus-induced airway inflammation will impact the management of diseases associated with airway hyperreactivity.


Subject(s)
Methacholine Chloride/immunology , Ovalbumin/immunology , Respiratory Hypersensitivity/immunology , Respiratory Hypersensitivity/virology , Respiratory Syncytial Virus Infections/immunology , Animals , Body Weight/immunology , Bronchial Provocation Tests , Eosinophils/immunology , Female , Inflammation/immunology , Lung/drug effects , Lung/immunology , Lung/pathology , Lymphocytes/immunology , Macrophages/immunology , Methacholine Chloride/pharmacology , Mice , Mice, Inbred BALB C , Ovalbumin/pharmacology , Respiratory Hypersensitivity/chemically induced , Specific Pathogen-Free Organisms , Time Factors , Viral Plaque Assay
19.
Compr Ther ; 24(10): 511-8, 1998 Oct.
Article in English | MEDLINE | ID: mdl-9801850

ABSTRACT

This article summarizes recent findings regarding the impact of viruses on reactive airway disease. Technical breakthroughs are improving our ability to diagnosis viral infections. However, the real breakthrough will come when pharmacologic interventions can specifically prevent the symptoms of virally induced asthma.


Subject(s)
Asthma/virology , Virus Diseases/diagnosis , Virus Diseases/therapy , Animals , Asthma/etiology , Diagnosis, Differential , Humans , Virus Diseases/complications
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