New molecular identifiers for Simulium limbatum and Simulium incrustatum s.l. and the detection of genetic substructure with potential implications for onchocerciasis epidemiology in the Amazonia focus of Brazil.

The Amazonia onchocerciasis focus of southern Venezuela and northern Brazil is the larger of the two remaining Latin American onchocerciasis foci where disease transmission still occurs and is often regarded as the most challenging of all the Latin American foci to eliminate onchocerciasis. The site is home to a population of over 20,000 semi-nomadic, hunter-gatherer Yanomami people and is made-up of a mosaic of rainforest and savannah ecologies, which are influenced by the area's undulating terrain and rich geological diversity. At least six blackfly vectors have been implicated in onchocerciasis transmission in this focus; however, because of the difficulty in their routine identification the relative importance of each has been obscured. Simulium limbatum and Simulium incrustatum s.l. have both been recorded as vectors in the Amazonia focus, but they are difficult to discriminate morphologically and thus the ecological range of these species, and indeed the presence of S. limbatum in the Amazonia focus at all, have remained controversial. In the work described here, we report 15 S. incrustatum s.l. CO1 sequences and 27 S. limbatum sequences obtained from field-caught adult female blackflies collected from forest and savannah localities, inside and just outside the Amazonia focus. Phylogenetic analysis with the sequences generated in this study, showed that both the S. limbatum and the S. incrustatum s.l. CO1 sequences obtained (even from specimens living in sympatry) all fell into discrete species-specific bootstrap-supported monophyletic groups and thus confirmed the utility of the CO1 gene for identifying both these species inside the Amazonia focus. As the S. limbatum-exclusive cluster included CO1 sequences obtained from forest-caught and morphologically identified specimens these results provide the clearest evidence yet of the presence of S. limbatum inside the Amazonia focus. The question, however, of whether S. limbatum is actually a vector in the focus still remains unanswered as the data presented here also suggest that S. limbatum found in the savannahs adjacent to, but outside the Amazonia focus (and which represent the only S. limbatum population to be unambiguously incriminated as a host of Onchocerca volvulus), are genetically distinct from those living inside the focus. These findings highlight the need for a clearer picture of the vector taxonomy inside the Amazonia onchocerciasis focus.

Vector competence for Onchocerca volvulus in the Simulium (Notolepria) exiguum complex: cytoforms or density-dependence?

Although Simulium exiguum Roubaud s.l. is present in all South American onchocerciasis foci, it is a significant vector only in Colombia and Ecuador. This variable vectorial role has been attributed to sibling forms that differ in their ability to allow Onchocerca volvulus larval development and their preferred bloodmeal hosts. Here we evaluate the relationship between parasite availability in human skin and infective larval output measured as (a) number of L3 larvae and (b) proportion of surviving flies with L3s in the Cayapa form of S. exiguum s.l. from Ecuador, taking into account the variation in counts of microfilariae (mf) from 6skin snips/patient. Comparisons with other cytoforms (Aguarico, Bucay and Quevedo, absent in the main Ecuadorean onchocerciasis foci) are made to suggest the relative roles of intrinsic susceptibility or co-adaptation versus density-dependent parasite uptake. A nonlinear (limitation) relationship, characterised by an initial rapid increase in infective larvae with increasing mf skin density was confirmed for the Cayapa cytoform. The proportion of infective Cayapa flies increased and saturated rapidly (reaching 80% for >/= 20mf/mg skin). After adjusting for density dependence, non-Cayapa cytoforms exhibited significantly lower L3 loads and proportions of infective flies for a given mf skin density than Cayapa flies, indicating that the susceptibility of those cytoforms is intrinsically lower than that of the Cayapa cytoform and that the differences observed are not due to density-dependent effects.

Human onchocerciasis in Brazil: an overview

The article reports on the history of the disease`s discovery, its distribution, and incrimination of vector simuliid species. The literature that has been generated on the parasite, its vectors, and control of the disease is critically analyzed as well as the organization of epidemiological surveys and the control program developed by the Brazilian government and an international agency. Suggestions for future work are made. (AU)

Human onchocerciasis in Brazil: an overview

Human onchocerciasis was recently discovered in Brazil among Yanomámi Indians living along the border region with Venezuela in the States of Amazonas and Roraima. The article reports on the history of the disease's discovery, its distribution, and incrimination of vector simuliid species. The literature that has been generated on the parasite, its vectors, and control of the disease is critically analyzed as well as the organization of epidemiological surveys and the control program developed by the Brazilian government and an international agency. Suggestions for future work are made

Human onchocerciasis in Brazil: an overview.

Human onchocerciasis was recently discovered in Brazil among Yanomámi Indians living along the border region with Venezuela in the States of Amazonas and Roraima. The article reports on the history of the disease's discovery, its distribution, and incrimination of vector simuliid species. The literature that has been generated on the parasite, its vectors, and control of the disease is critically analyzed as well as the organization of epidemiological surveys and the control program developed by the Brazilian government and an international agency. Suggestions for future work are made.

Onchocerciasis in Ecuador: the situation in 1989

Details are given of the prevalence rates of onchocerciasis from the most recent surveys (1989) conducted in northern Ecuador. The disease has intensified and dispersed considerably due to migration of infected individuals and the presence of a highly efficient vector. Comparison of these data with those from two previous surveys carried out in 1982/83 and 1986 and correlated with entomological findings highly the danger of the formation of new foci of onchocerciasis in areas currently free of the disease. Recommendations are made for further entomological studies in areas either recently or likely to be affected by the disease where potential vectors are unknown or different to those registred in the Santiago focus. Invermectin treatment with local vector control in specific areas is advocated to reduce the disease to a low level of public health importance

Identification of vector species (Diptera: Simuliidae) of human onchocerciasis in the Amazonia focus of Brazil and Venezuela

Revisamos as posiçöes taxonômicas de três espécies de simulídeos da Amazônia, Simulium guianense Wise, S. oyapockense Flock & Abonnenc e S. yarzabali Ramirez Perez. Sinonimizamos S. cuasisanguineum Ramires Perez, Yarzabal & Peterson com S. oyapockense, revalidamos S. yarzabali que estava em sinonímia com S. incrustatum. Discutimos os papéis destas três espécies na transmissäo de oncocercose e mansonelose humana na Amazônia

O foco brasileiro de oncocercose: novas observaçöes feitas nas áreas dos rios Mucajaí e Catrimâni, Território de Roraima / The brazilian focus of onchocerciasis: new observations in areas of the Mucajaí and Catrimâni, Territory of Roraima

Um novo inquérito para oncocercose, realizado em 1984, entre índios Yanomami da parte média dos rios Mucajaí e Catrimâni (Território de Roraima), mostrou que, decorridos vários anos das primeiras investigaçöes - uma década no caso do rio Mucajaí - os índices de prevalência, nesses dois locais da periferia do foco brasileiro, näo haviam sofrido alteraçäo significativa. Levando-se em conta apenas os residentes nas aldeias ou malocas abrangidas pelo inquérito, a prevalência atingiu 3,1% nos índios do rio Mucajaí, enquanto ficou em zero nos do rio Catrimâni. Dada a presença contínua, nas referidas aldeias, de índios visitantes, altamente infectados, oriundos da parte central e mais elevada do território indígena - onde cerca de 90% doa adultos têm oncocercose - seria de esperar o achado de valores bem maiores (acima pelo menos daqueles encontrados anteriormente), caso um vetor apropiado estivesse presente na regiäo. Simulium oyapockense s.1. é a única espécie antropofílica de simulídeo, em toda zona inferior da área ocupada pelos Yanomami (altitude ao redor de 200 metros), abundante o suficiente para constituir-se em transmissor da oncocercose. Sem dúvida, no entanto, trata-se de um mau vetor (como aliás já foi demonstrado experimentalmente para Mansonella ozzardi) ou, até mesmo, de espécie näo vetora de Onchocerca volvulus, pois, de outra forma, os índices de prevalência na parte média dos rios Mucajaí e Catrimâni já teriam crescido durante o período assinalado. Para explicar as altas taxas alcançadas pela endemia na porçäo central e cheia de acidentes (altitude superior a 900 metros) do território Yanomami, há que se admitir a presença aí de um outro vetor, muito eficiente, cujos hábitos estariam ligados à regiäo montanhosa da fronteira entre o Brasil e a Venezuela

Mansonella ozzardi no Território Federal de Roraima, Brasil: distribuiçäo e achado de um novo vetor na área do rio Surumu / Mansonella ozzardi in the Federal Territory of Roraima, Brazil: distribution and finding of a new vector in the area of Surumu River

Um inquérito hemoscópico, conduzido entre indígenas Makuxi, pertencentes a 15 localidades da regiäo nordeste do Território Federal de Roraima, revelou a presença de microfilárias de Mansonella ozzardi em 3,2% das 652 pessoas examinadas. O número de microfilárias nos positivos era pequeno, näo ultrapassando a 18 nas amostras de sangue(20mm3) colhidas. A baixa densidade na microfilaremia e a inexistência da infecçäo em menores de 15 anos sugerem que a filariose está sendo adquirida pelos indígenas fora de suas aldeias, provavelmente nos garimpos de ouro situados na parte alta do rio Maú. Simulium oyapockense s.l. ou Simulium roraimense - espécie muito espalhada no norte do Território - foi reconhecido como vetor de M. ozzardi na área do rio Surumu. As fêmeas desse simulídeo, embora capazes de suportar o desenvolvimento da filária até L3, näo se infectam com facilidade. Assim, numa tentativa de infecçäo experimental, apenas 20,6% das fêmeas alimentadas sobre um voluntário (com 12mf/20mm3 de sangue colhido da polpa digital) exibiram estádios larvares de M. ozzardi (1-2 larvas somente por exemplar infectado). A alta prevalência em índios Sanumá e Mayongong, que vivem no lado oposto do Território, aponta para a existência, na área do rio Auaris, onde se localizam esses indígenas, de um outro vetor, muito mais eficiente do que S. oyapockense s.l