Long-term response of interspecific competition among three typical bloom-forming species to changes in phosphorus and temperature.

Phosphorus and temperature play an important role in the succession of diatom-dinoflagellate blooms. However, there is little long-term research on interspecific competition based on phosphorus source and temperature. Here, interspecific competition among Skeletonema costatum, Prorocentrum donghaiense and Karenia mikimotoi was studied using trialgal laboratory co-cultures under different phosphorus and temperature conditions. These results suggest that S. costatum and P. donghaiense alternated as competing dominant species during the experimental period, which coincides with the different phosphorus conditions. However, K. mikimotoi growth was significantly inhibited throughout the experiment. We suggest that this may be due to different algal requirements for phosphorus, optimal growth temperatures, and possible allelopathic effects. This study provides a comprehensive mechanism of interspecific competition between diatom-dinoflagellate in response to phosphorus and temperature and elucidates the seasonal succession of diatom-dinoflagellate from late spring to early summer in the Changjiang River Estuary and the adjacent East China Sea.

Changes in dinoflagellate and diatom blooms in the East China Sea over the last two decades, under different spatial and temporal scale scenarios.

Frequent algal blooms in the nearshore area of the East China Sea (ECS) pose a serious threat to both the marine environment and human health. Climate and environmental changes play an important role in the occurrence of diatoms and dinoflagellates blooms. Using the MODIS-Aqua 1-km satellite observations, the outbreaks of dinoflagellate and diatom blooms in the ECS coast in summer during 2003-2022 were mapped. Our results found that although the bloom frequency of dinoflagellate was consistently higher than diatoms, its bloom intensity showed a slightly decline trend in recent decades. The driving factors analysis showed that river runoff and sediments discharge played different effect on the formation of diatom and dinoflagellate blooms. Besides, our results compared the effect of El Niño and La Niña on bloom occurrences. This study was supposed to provide detailed insights into algal blooms, with important implications for relevant meteorological and climate changes in coastal regions.

Transcriptome analysis of the bloom-forming dinoflagellate Prorocentrum donghaiense exposed to Ginkgo biloba leaf extract, with an emphasis on photosynthesis.

Ginkgo biloba leaf extract (GBE) can effectively treat bloom-forming freshwater algae. However, there is limited information about the underlying suppression mechanism of the marine bloom-forming Prorocentrum donghaiense-the most dominant algal bloom species in the East China Sea. We investigated the effect of GBE on P. donghaiense in terms of its response to photosynthesis at the molecular/omic level. In total, 93,743 unigenes were annotated using six functional databases. Furthermore, 67,203 differentially expressed genes (DEGs) were identified in algae treated with 1.8 g∙L-1 GBE. Among these DEGs, we identified the genes involved in photosynthesis. PsbA, PsbB and PsbD in photosystem II, PsaA in photosystem I, and PetB and PetD in the cytochrome b6/f complex were downregulated. Other related genes, such as PsaC, PsaE, and PsaF in photosystem I; PetA in the cytochrome b6/f complex; and atpA, atpD, atpH, atpG, and atpE in the F-type H+-ATPase were upregulated. These results suggest that the structure and activity of the complexes were destroyed by GBE, thereby inhibiting the electron flow between the primary and secondary quinone electron acceptors, primary quinone electron acceptor, and oxygen-evolving complex in the PSII complex, and interrupting the electron flow between PSII and PSI, ultimately leading to a decline in algal cell photosynthesis. These findings provide a basis for understanding the molecular mechanisms underlying P. donghaiense exposure to GBE and a theoretical basis for the prevention and control of harmful algal blooms.

Oscillations of algal cell quota: Considering two-stage phosphate uptake kinetics.

Elucidating the mechanism of effect of phosphate (PO43-) uptake on the growth of algal cells helps understand the frequent outbreaks of algal blooms caused by eutrophication. In this study, we develop a comprehensive mathematical model that incorporates two stages of PO43- uptake and accounts for transport time delay. The model parameter values are determined by fitting experimental data of Prorocentrum donghaiense and the model is validated using experimental data of Karenia mikimotoi. The numerical results demonstrate that the model successfully captures the general characteristics of algal growth and PO43- uptake under PO43- sufficient conditions. Significantly, the experimental and mathematical findings suggest that the time delay associated with the transfer of PO43- from the surface-adsorbed PO43- (Ps) pool to the intracellular PO43- (Pi) pool may serve as a physiologically plausible mechanism leading to oscillations of algal cell quota. These results have important implications for resource managers, enabling them to predict and deepen their understanding of harmful algal blooms.

Allelopathic Inhibition and Mechanism of Quercetin on Microcystis aeruginosa.

The utilization of allelochemicals to inhibit algal overgrowth is a promising approach for controlling harmful algal blooms (HABs). Quercetin has been found to have an allelopathic effect on algae. However, its responsive mechanism needs to be better understood. In the present study, the inhibitory effects of different quercetin concentrations on M. aeruginosa were evaluated, and the inhibition mechanisms were explored. The results demonstrated that quercetin significantly inhibited M. aeruginosa growth, and the inhibitory effect was concentration-dependent. The inhibition rate of 40 mg L-1 quercetin on algal density reached 90.79% after 96 h treatment. The concentration of chlorophyll-a (chl-a) in treatment groups with quercetin concentrations of 10, 20, and 40 mg L-1 decreased by 59.74%, 74.77%, and 80.66% at 96 h, respectively. Furthermore, quercetin affects photosynthesis and damages the cell membrane, respiratory system, and enzyme system. All photosynthetic fluorescence parameters, including the maximum photochemical quantum yield (Fv/Fm), the actual photochemical quantum yield (YII), the maximum relative electron transfer rate (rETRmax), and light use efficiency (α), exhibited a downtrend after exposure. After treatment with 20 mg L-1 quercetin, the nucleic acid and protein content in the algal solution increased, and the respiration rate of algae decreased significantly. Additionally, superoxide dismutase (SOD) activities significantly increased as a response to oxidative stress. In comparison, the activities of ribulose 1,5-biphosphate carboxylase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC) decreased significantly. These results revealed that quercetin could inhibit M. aeruginosa by affecting its photosynthesis, respiration, cell membrane, and enzymic system. These results are promising for controlling M. aeruginosa effectively.

Effects of Ginkgo biloba extract on growth, photosynthesis, and photosynthesis-related gene expression in Microcystis flos-aquae.

The inhibitory effect of plants on algae offers a new and promising alternative method for controlling harmful algal blooms. Previous studies showed that anti-algal effects might be obvious from extracts of fallen leaves from terrestrial plants, which had great potential for cyanobacterial control in field tests. To investigate the anti-algal activities and main algicidal mechanisms of Ginkgo biloba fallen leaves extracts (GBE) on Microcystis flos-aquae, the cell density, photosynthetic fluorescence, and gene expression under different concentrations of GBE treatments were tested. GBE (3.00 g L-1) showed a strong inhibitory effect against M. flos-aquae with an IC50 (96h) of 0.79 g L-1. All the inhibition rates of maximal quantum yield (Fv/Fm), effective quantum yield (Fq'/Fm'), and maximal relative electron transfer rate (rETRmax) were more than 70% at 96 h at 3.00 g L-1 and more than 90% at 6.00 g L-1. Further results of gene expression of the core proteins of PSII (psbD), limiting enzyme in carbon assimilation (rbcL), and phycobilisome degradation protein (nblA) were downregulated after exposure. These findings emphasized that photosynthetic damage is one of the main toxic mechanisms of GBE on M. flos-aquae. When exposed to 12.00 g L-1 GBE, no significant influence on the death rate of zebrafish or photosynthetic activity of the three submerged plants was found. Therefore, appropriate use of GBE could control the expansion of M. flos-aquae colonies without potential risks to the ecological safety of aquatic environments, which means that GBE could actually be used to regulate cyanobacterial blooms in natural waters.

The effect of plant extracts on growth and photosynthetic fluorescence characteristics of Microcystis flos-aquae.

The cyanobacteria Microcystis flos-aquae can cause harmful algal blooms in waterbodies, which threaten the normal functioning of aquatic ecosystems and human health. Some plant extracts are considered as promising algaecides. In this study, the effects of ten plant extracts (Cinnamomum camphora, Ginkgo biloba, Firmiana platanifolia, Salix babylonica, Euphorbia humifusa, Erigeron annuus, Solidago canadensis, Alternanthera philoxeroides, Thalia dealbata and Eichhornia crassipes) against M. flos-aquae were investigated. The results showed that all ten plant extracts had a significant inhibitory effect on M. flos-aquae growth after 96 h (P < 0.01). The inhibition rates of S. babylonica, E. humifusa, S. canadensis and A. philoxeroides were over 70.00%. Furthermore, the E. humifusa extract had the best inhibitory effect on the photosynthesis of M. flos-aquae, with the effective quantum yield of photosystem II and maximal relative electron transport rate decreasing by 97.50% and 97.00%, respectively, after 96 h. Additionally, the E. humifusa extract was found to be non-toxic to non-target organisms such as Brachydanio rerio and Vallisneria spiralis within 96 h. This study contributes to the existing knowledge and data of freshwater cyanobacteria blooms, and provides insights for their control and the restoration of freshwater systems affected by cyanobacteria blooms.

Changes in community structure and photosynthetic activities of total phytoplankton species during the growth, maintenance, and dissipation phases of a Prorocentrum donghaiense bloom.

The potential interactions between the bloom-forming dinoflagellates and other phytoplankton during the algal bloom cycle are interesting, while the causes for the phytoplankton community changes were not fully understood. We hypothesized that phytoplankton community structure and photosynthetic activities of total phytoplankton have their special characteristics in different phases of the algal blooms. To test this hypothesis, a survey covering the process of a Prorocentrum donghaiense bloom in coastal waters between Dongtou and Nanji Islands was carried out from 9 to 20 May 2016, and the changes in the phytoplankton community and photosynthetic activities of total phytoplankton were determined. Surface seawater was sampled for microscopic analysis of phytoplankton composition and pulse amplitude modulated (PAM) chlorophyll fluorescence analysis of photosynthetic activities of the total phytoplankton species. A total of 25, 31, and 19 phytoplankton species were identified in its growth (9-12 May), maintenance (13-18 May) and dissipation phases (19-20 May), respectively. Diatoms were dominant in terms of species number while dinoflagellates were predominant at cell abundance. Dinoflagellates were the major dominant species during three phases of the bloom based on the dominance (Y) value, whereas the dominant species extended to dinoflagellates and diatoms including P. donghaiense, Coscinodiscus argus, Gonyaulax spinifera, Cyclotella sp. and Scrippsiella trochoidea in the dissipation phase. In the maintenance phase, the average cell abundances of the total phytoplankton and P. donghaiense were consistent with the chlorophyll a (Chla) concentration in the seawater; for the diversity indices of total phytoplankton species, Simpson index (C) was the highest while Shannon index (H') and Pielou evenness index (J') were the lowest. Furthermore, photosynthetic activities of the total phytoplankton species represented by the effective quantum yield (Fq'/Fm') and the maximum relative electron transport rate (rETRmax) in the maintenance phase were higher than those in the growth and dissipation phases. The results indicated that the characteristics of phytoplankton community structure and photosynthetic activities could be regarded as criteria in predicting the phases of algal blooms.

Regulation of phosphate uptake kinetics in the bloom-forming dinoflagellates prorocentrum donghaiense with emphasis on two-stage dynamic process.

Phosphorus is an essential element for the growth and reproduction of algae. In recent years, the frequent outbreaks of algal blooms caused by eutrophication have drawn much attention to the influence of phosphate (P) uptake on the growth of algal cells. The previous study only considered the effect of total P pools on the P uptake process of algal cells and considered the process as one stage, which is insufficient. P uptake by algae is actually a two-stage kinetic process because in many algae species, surface-adsorbed P pools account for a large proportion of total P pools. In this paper, we fit one-stage and two-stage models of P uptake by algae to our experimental data on short-term uptake kinetics of algae Prorocentrum donghaiense under P-deplete and P-replete conditions at 24°C. According to the experimental results, P. donghaiense possesses different P uptake characteristics under different P concentrations. P. donghaiense grows faster and exponentially for longer periods of time under P-replete condition. Ranges of change of Qc (cell quota of intracellular P) and Sp (cell quota of surface-adsorbed P) during the culture time are obviously larger under P-replete condition than those under P-deplete condition. The value of Kp (represents the impact of P-starvation on P uptake rate) in one-stage model under P-deplete condition is smaller than that under P-replete condition, which is opposite to results of two-stage model and does not meet the actual biological significance of Kp. The two-stage model gives more reasonable and realistic explanations to the process of P uptake by algae no matter from the perspective of intuitive fitting effect, biological significance of parameters, statistical test results or essential dynamic process. These results, combined with long-term lab and field data in ocean, could be used to effectively predict algal blooms.

[Pelagic Mysidacea in East China Sea and its significance as the indicator of water mass].

Based on the investigation data of four seasonal oceanographic censuses in the area 23 degrees 30' - 33 degrees N and 118 degrees 30' - 128 degrees E of East China Sea in 1997-2000, this paper studied the ecotypes and geographic and seasonal distribution of pelagic Mysidacea in this sea area, with its significance as the indicator of water mass approached. The results showed that among the species of pelagic Mysidacea, Acanthomysis latiscauda, Gastrosaccus pelagicus, Siriella sinensis and Hemisiriella pulchra were the coastal subtropical species with some features of warm temperate species, while Pseudanchialina pusilla, Promysis orientalis, Erythrops minuta and Anisomysis bipartoculata were the offshore subtropical species with some features of tropical species. Different from other zooplanktons, most of the pelagic Mysidacea species had lower optimal temperature and salinity and distributed more at near-shore in spring and winter, showing the features of warm temperate near-shore species. The areas with high abundance of A. latiscauda, S. sinensis and H. pulchra were just the converging areas of the Changjiang dilute waters and warm current masses and of coastal waters and offshore high salinity waters. Erythrops minuta and Anisomysis bipartoculata could be used as the indicators of warm current, P. orientalis and Doxomysis quadrispinosa were the indicators of the Taiwan warm current, while the rest were not good enough to be the indicators of water masses.