The effect of melatonin on the mouse ameloblast-lineage cell line ALCs.

Melatonin plays a critical role in promoting the proliferation of osteoblasts and the growth and development of dental papilla cells. However, the effect and mechanism of melatonin on the growth and development of ALCs still need to be explored. CCK8 assay was used for the evaluation of cell numbers. qRT-PCR was used to identify the differentially expressed genes in ALCs after melatonin treatment. The number and morphology of ALCs were investigated by confocal microscopy. Alkaline phosphatase assay and Alizarin red S staining were used for measuring mineralization. Then, we focused on observing the crucial factors of the signaling pathway by RNA-seq and qRT-PCR. Melatonin limited the cell number of ALCs in a dose-dependent manner and promoted the production of actin fibers. A high concentration of melatonin significantly promoted the mRNA levels of enamel matrix proteins and the formation of mineralized nodules. RNA-seq data showed that Wnt signaling pathway may be involved in the differentiation of ALCs under the influence of melatonin. This study suggests that melatonin plays a regulatory role in the cell number, differentiation, and mineralization of the ALCs, and then shows the relationship between the Wnt signaling pathway with the ALCs under melatonin.

A modified dental age assessment method for 5- to 16-year-old eastern Chinese children.

OBJECTIVES: Age estimation is widely applied in the field of orthodontics, pediatric dentistry, and forensic science. Dental age estimation by the radiological method is frequently used because of its convenience and noninvasiveness. However, there are not enough suitable methods for eastern Chinese children. This study aimed to establish a modified formula for eastern Chinese children according to the Demirjian method and then compared the accuracy of the modified method with the Demirjian method and Willems method. MATERIALS AND METHODS: A total of 2367 dental panoramic radiographs from individuals aged 5-16 years of eastern China were collected as samples. Age estimation was conducted using the Demirjian and Willems methods. The polynomial curve fitting method was used to modify the Demirjian method to improve its application to the eastern Chinese children. The paired t test and accuracy ratio were used to compare the applicability of the modified methods with two commonly used methods. RESULTS: The mean chronological age (CA) of the subjects was 11.20 ± 3.29 years for boys and 10.99 ± 3.12 years for girls. The mean difference values between the CA and dental age (DA) (CA-DA) using the Demirjian and Willems methods were 0.73 and 0.7 for boys, respectively, and both 0.79 for girls. The modified method using the polynomial curve fitting presented a smaller underestimation compared with CA for both boys (0.04 years) and girls (0.09 years), which showed a high suitability to Chinese children to some extent. CONCLUSIONS: The Willems method was more accurate in estimating DA compared with the Demirjian method. However, the modified method was more accurate than the two methods; therefore, it can be used in eastern Chinese children. CLINICAL RELEVANCE: It was thought to be a non-invasive, convenient, and efficient method to connect DA and CA. By estimating dental age, pediatrist, and orthodontists can better understand the development of permanent teeth and provide a more accurate orthodontic treatment time and treatment plan to children patients.

Applicability of 2 Dental Age Estimation Methods to Taiwanese Population.

The aim of this study is to assess the accuracy of Demirjian and Willems dental age estimation methods on a population of 8- to 15-year-old Taiwanese children. A total of 799 digital panoramic radiographs (404 boys, 395 girls) of children aged from 8.00 to 15.99 years are evaluated. All panoramic films are assessed by 2 examiners using Demirjian and Willems methods. We randomly select 48 panoramic films to evaluate the reproducibility of dental age measurements. The intraobserver and interobserver Cohen's kappa values are 0.733 and 0.762, respectively.The difference between chronological age and dental age is calculated for each individual using both methods. The result shows that Demirjian method slightly overestimates the chronological age of children. The mean age difference is 0.12 years for boys (standard deviation [SD], 0.80) and 0.21 years for girls (SD, 0.95). Willems method slightly underestimates the chronological age of children. The mean age difference is -0.22 years for boys (SD, 0.80) and -0.12 years for girls (SD, 0.92).In conclusion, Demirjian and Willems methods are both accurate for assessing dental age in the Taiwan region of China. The mean age differences of both methods are within ±1 year, which is acceptable at forensic odontology level. However, although the discrepancy between chronological age and dental age is frequently observed between -0.5 and 0.5 years in both methods, there is no guarantee it will be accurate for any particular individual, further modifications to both methods are suggested.