Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 8 de 8
Filter
Add more filters










Database
Publication year range
1.
JSLS ; 20(4)2016.
Article in English | MEDLINE | ID: mdl-27904310

ABSTRACT

BACKGROUND AND OBJECTIVES: Patients with previous gastrectomy have an increased incidence of gallstones and gallbladder morbidity requiring surgery. We investigated the possible risk factors that contribute to severe gallbladder disease in patients with previous gastrectomy and the role of laparoscopic cholecystectomy (LC) in the treatment of these patients. METHODS: In this retrospective study, we reviewed a database of patients who underwent LC in our hospital during the period January 1, 2010, through May 1, 2015. RESULTS: The average operation time in patients with previous gastrectomy was longer (P < .05), but the operation times of patients with a long interval (>5 years) between gastrectomy and LC showed no statistical difference from those of patients without a history of gastrectomy (P > .05). The conversion rate did not differ between the 2 groups (P > .05), but in patients with previous gastrectomy, the conversion rate was significantly reduced after we adopted a comprehensive preoperative evaluation procedure (P < .05). The frequency of cholecystitis attacks, rate of combination with gallbladder polyps, and rate of combination with pancreatitis were higher and gallstone formation time shorter, in the patients with malignant tumor, those undergoing Billroth II gastroenterostomy or esophagojejunostomy, and those with accompanying diabetes mellitus or hypercholesterolemia (P < .05). CONCLUSION: LC plays an important role in the treatment of benign gallbladder diseases in patients with a history of gastrectomy, and a comprehensive preoperative evaluation and accomplished surgical technique are necessary for successful outcomes. Previously identified clinical features may represent a risk factor for severe cholecystic morbidity in these patients.


Subject(s)
Cholecystectomy, Laparoscopic/methods , Cholecystitis/surgery , Gastrectomy , Stomach Neoplasms/surgery , Adult , Aged , Aged, 80 and over , Cholecystitis/complications , Female , Gallbladder Diseases/surgery , Humans , Male , Middle Aged , Retrospective Studies , Risk Factors , Stomach Neoplasms/complications , Young Adult
2.
World J Gastroenterol ; 20(16): 4835-8, 2014 Apr 28.
Article in English | MEDLINE | ID: mdl-24782641

ABSTRACT

Splenic artery aneurysm is one of the most common visceral aneurysms, and patients with this type of aneurysm often present without symptoms. However, when rupture occurs, it can be a catastrophic event. Although most of these aneurysms can be treated with percutaneous embolization, some located in uncommon parts of the splenic artery may make this approach impossible. We present a patient with an aneurysm in the proximal splenic artery, close to the celiac trunk, which was treated by laparoscopic ligation only, without resection of the aneurysm, and with long-term preservation of splenic function.


Subject(s)
Aneurysm/surgery , Laparoscopy , Splenic Artery/surgery , Aneurysm/diagnosis , Female , Humans , Ligation , Middle Aged , Splenic Artery/diagnostic imaging , Tomography, X-Ray Computed , Treatment Outcome , Ultrasonography, Doppler, Color
3.
Vaccine ; 30(23): 3395-404, 2012 May 14.
Article in English | MEDLINE | ID: mdl-22480929

ABSTRACT

Human telomerase reverse transcriptase (hTERT) has been identified as an ideal tumor-associated antigen (TAA). Use of a synthetic hTERT epitope peptide to pulse dendritic cells can induce autologous T cell anti-tumor immune responses, but such responses induced by a single epitope peptide have been shown to be weak and a narrow-spectrum. Here, we designed dendritic tandem multiple antigenic peptides (MAPs) containing the following three hTERT epitope peptides: I540, V461 and L766, which are HLA-A*02-, HLA-A*24- and HLA-RDB1*04/11/15-restricted, respectively. The MAPs and their three single-epitope peptides were obtained through solid-phase synthesis. Healthy volunteers that were HLA-A*02(+)/HLA-DRB1*04(+) and HLA-A*24(+)/HLA-DRB1*15(+) were recruited. Myeloid dendritic cells were isolated by magnetic activated cell sorting and were divided into a MAP-stimulated group (MAP-DC), a group in which the three epitope peptides were mixed and used to stimulate the DCs (MixP-DC) and a no peptide-stimulated group (NoP-DC, control group). All of the DCs were cultured in serum-free medium, pulsed with the corresponding peptides on the 3rd, 5th and 7th days, and co-cultured with autologous lymphocytes when they were mature. The related cytokines were measured via ELISA. The killing effects of cytotoxic T lymphocytes (CTLs) on SW480/A549 tumor cells expressing HLA-A*02(+), HepG2/SMMC-7721 cells expressing HLA-A*24(+) and SKOV3 cells negative for HLA-A*02/A*24 were detected by flow cytometry. Our results indicated that the CTLs induced by the MAP-DCs had the greatest anti-tumor effect. Therefore, the dendritic tandem multiple antigenic hTERT epitope peptides combined with MDCs may represent a powerful, broad-spectrum anti-tumor vaccine.


Subject(s)
Cancer Vaccines/immunology , Dendritic Cells/immunology , Epitopes/immunology , Telomerase/immunology , Adult , Cancer Vaccines/administration & dosage , Cancer Vaccines/genetics , Cells, Cultured , Coculture Techniques , Epitopes/genetics , HLA Antigens/genetics , HLA Antigens/immunology , Humans , T-Lymphocytes, Cytotoxic/immunology , Telomerase/genetics , Vaccines, Synthetic/administration & dosage , Vaccines, Synthetic/genetics , Vaccines, Synthetic/immunology
5.
Am Surg ; 77(11): 1496-501, 2011 Nov.
Article in English | MEDLINE | ID: mdl-22196664

ABSTRACT

About 70 per cent of patients with hepatocellular carcinoma are diagnosed at intermediate or advanced stages, and most of them are technically unresectable. As a novel, emerging therapeutic modality, high intensity focused ultrasound (HIFU) has a great potential for tumor treatment. In this review, principle of HIFU technique is introduced, and an overview of clinical applications and limitations of HIFU for HCC treatment, as well as prospects for future development, is provided. Consequently, HIFU has been considered a safe and feasible procedure for HCC treatment.


Subject(s)
Carcinoma, Hepatocellular/surgery , Liver Neoplasms/surgery , Ultrasonic Surgical Procedures/methods , Carcinoma, Hepatocellular/pathology , Feasibility Studies , Humans , Liver Neoplasms/pathology , Neoplasm Recurrence, Local/prevention & control , Neoplasm Staging , Treatment Outcome
6.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 27(6): 626-30, 2011 Jun.
Article in Chinese | MEDLINE | ID: mdl-21651861

ABSTRACT

AIM: To study the antigen specific anti-tumor effect of cytotoxic T lymphocytes(CTLs), which was induced by human telomerase reverse transcriptase (hTERT)-related multiple epitope peptides impulsed myeloid dendritic cells(mDCs), against human leukocyte antigen (HLA)-A24(+) tumor cells. METHODS: Four branches of multiple antigen peptides (MAPs) of hTERT epitopes and three separate peptides were solid-phase artificially synthesized, phlebotomize peripheral blood from HLA-A24(+) healthy volunteers, sorted the blood through MACS MicroBeads and cultured mDCs, Nylon fiber column purified T lymphocytes, mDCs impulsed with each type of peptides were co-cultured with T lymphocytes to induce CTLs specifically killing effect, and the resultant CTLs were used as effector cells, SMMC-7721 with hTERT and HLA-A24 positive and SKOV3 which are hTERT-positive but HLA-A24-negative tumor cells were used as target cells. The level of human IL-12, TNF-α in the culture supernatant was determined by ELISA. Flow cytometry assay was used to assess the killing ability of CTLs against tumor cells. RESULTS: MAPs of hTERT epitopes including I540 (ILAKFLHWL), V461 (VYGFVRACL), L766 (LTDLQPYMRQFVAHL) and three separate peptides could impulse mDCs and then induce CTLs to specifically kill SMMC-7721, CTLs induced by MAPs had stronger cytotoxic effect compared with three separate peptides mixed(P < 0.05). CONCLUSION: mDCs-impulsed with hTERT-associated MAPs can induce production and proliferation of allogenic CTLs, which show antigen specific anti-tumor effect against HLA-A24(+) tumor cells. This result has significantly meaning in tumor immunotherapy.


Subject(s)
Carcinoma, Hepatocellular/immunology , Cell Death/immunology , Dendritic Cells/drug effects , Liver Neoplasms/immunology , Peptide Fragments/administration & dosage , T-Lymphocytes, Cytotoxic/immunology , Telomerase/genetics , Adult , Antigens, Neoplasm/immunology , Carcinoma, Hepatocellular/therapy , Cell Line, Tumor , Cytotoxicity, Immunologic , Dendritic Cells/cytology , Dendritic Cells/immunology , Epitopes/immunology , Female , HLA-A Antigens/metabolism , HLA-A24 Antigen , Humans , Immunotherapy , Interleukin-12/metabolism , Liver Neoplasms/therapy , Peptide Fragments/chemical synthesis , Peptide Fragments/immunology , T-Lymphocytes, Cytotoxic/drug effects , Tumor Necrosis Factor-alpha/metabolism
7.
Huan Jing Ke Xue ; 30(7): 2053-8, 2009 Jul 15.
Article in Chinese | MEDLINE | ID: mdl-19775007

ABSTRACT

Bio-availability of Cu and Zn fixed by phosphate in contaminated soils with application of nutrients were measured by pot experiment. It was simulated for the third national standardization of copper and zinc polluted soils by adding copper and zinc nitrate into red and paddy soils, respectively and together. Phosphate amendment was added to the soils to fix Cu and Zn, then added KCl and NH4Cl or K2SO4 and (NH4)2SO4 fertilizers following to plant Ryegrass, which was harvested after 40 d. Available Cu/Zn content in soils and biomass, Cu/Zn content in the shoot of Ryegrass were determined. Results showed that, compared with no nutrient application, adding KCl and NH4 Cl/K2SO4 and (NH4)2SO4 to polluted red and paddy soils increased the available Cu and Zn content in red soil significantly. The increasing order was KCl and NH4 Cl > K2SO4 and (NH4)2SO4. Especially in single Zn polluted red soil, the available Zn content increased by 133.4% in maximum. Although adding K2SO4 and (NH4)2SO4 could promote the growth of Ryegrass on red soil, and the largest increasing was up to 22.2%, it increased Cu and Zn content in the shoot of Ryegrass for 21.5%-112.6% remarkably. These nutrient effects on available Cu and Zn were not significantly in paddy soil. It was suggested that application of nitrogen and potassium fertilizers to soils could change the bioavailability of Cu/Zn. So it is necessary to take full account of the nutrient influence to the heavy metal stability which fixed by phosphate in contaminated soils when consider contaminated soils remediation by fertilization.


Subject(s)
Copper/chemistry , Environmental Restoration and Remediation/methods , Phosphates/chemistry , Soil Pollutants/chemistry , Zinc/chemistry , Ammonium Chloride/chemistry , Fertilizers , Lolium/growth & development , Potassium Chloride/chemistry
8.
Sheng Wu Gong Cheng Xue Bao ; 19(4): 489-92, 2003 Jul.
Article in Chinese | MEDLINE | ID: mdl-15969071

ABSTRACT

Clinical evidence of hematopoietic restoration with umbilical cord blood (UCB) grafts indicates the UCB can be a useful source of hematopoietic stem cells for routine bone marrow reconstitution. Considering (10 +/- 5) x 10(8) nucleared cells per cord blood unit, there is a potential limitation for the use of cord blood in adults, which, however, can be overcome by ex vivo expansion of cells. A prerequisite for expansion is the significantly higher recovery of MNC, CD34+ cells and colony-forming cells (CFC) by thawing cryopreserved MNC. Cooling rate always acts as a critical factor that can affect the recovery of cells. Although the rate of - 1 degrees C/min is adopted in most of the cryopreservations, no data has been reported about the detailed effects of different cooling rates. The aim of the study was to reveal the different effects of cooling rates on cryopreservation of hematopoietic stem cells from cord blood. UCB samples were collected, and cryopreserved as mononuclear cells (MNC) with different cooling rates of - 0.5 degrees C/min, - 1 degrees C/min, - 5 degrees C/min, and the recovery and viability of MNC and CD34+ cells, the clonogenic capacity and the ex vivo expansion potential of UCB progenitor cells were evaluated after thawing. With - 1 degrees C/min cooling rate, the recovery of MNC reached 93.3% +/- 1.8% , viability 95.0% +/- 3.9% , recovery of CD34+ cells 80.0% +/- 17.9% , and clonogenic recovery were 87.1% +/- 5.5%, 88.5% +/- 8.9%, 86.2% +/- 7.4% for BFU-E CFU-GM CFU-MK, respectively. After 14 days of liquid culture, no significant difference was detected in CFC expansion between fresh and cryopreserved MNC cells with - 1 degrees C/min cooling rate, but this was not the case with - 0.5 degreesC/min and - 5 degrees C/min. In conclusion, it was demonstrated that controlling the rate at - 1 degrees C/min is more suitable for cryopreservation of hematopoietic stem cells than - 0.5 degrees C/min and - 5 degrees C/min.


Subject(s)
Cryopreservation/methods , Fetal Blood/cytology , Hematopoietic Stem Cells/cytology , Cell Survival/physiology , Cells, Cultured , Erythroid Precursor Cells/cytology , Flow Cytometry , Granulocyte-Macrophage Progenitor Cells/cytology , Humans
SELECTION OF CITATIONS
SEARCH DETAIL
...