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1.
Mikrochim Acta ; 191(1): 41, 2023 12 19.
Article in English | MEDLINE | ID: mdl-38112843

ABSTRACT

A highly sensitive tumor necrosis factor α (TNF-α) detection method based on a surface-enhanced Raman scattering (SERS) magnetic patch sensor is reported. Magnetic beads (MNPs) and core shells were used as the capture matrix and signaling probe, respectively. For this purpose, antibodies were immobilized on the surface of magnetic beads, and then Au@4-MBN@Ag core-shell structures coupled with aptamers and TNF-α antigen were added sequentially to form a sandwich immune complex. Quantitative analysis was performed by monitoring changes in the characteristic SERS signal intensity of the Raman reporter molecule 4-MBN. The results showed that the limit of detection (LOD) of the proposed method was 4.37 × 10-15 mg·mL-1 with good linearity (R2 = 0.9918) over the concentration range 10-12 to 10-5 mg·mL-1. Excellent assay accuracy was also demonstrated, with recoveries in the range 102% to 114%. Since all reactions occur in solution and are separated by magnetic adsorption of magnetic beads, this SERS-based immunoassay technique solves the kinetic problems of limited diffusion and difficult separation on solid substrates. The method is therefore expected to be a good clinical tool for the diagnosis of the inflammatory biomarker THF-α and in vivo inflammation screening.


Subject(s)
Aptamers, Nucleotide , Tumor Necrosis Factor-alpha , Silver/chemistry , Gold/chemistry , Magnetics , Aptamers, Nucleotide/chemistry
2.
Food Chem ; 409: 135363, 2023 May 30.
Article in English | MEDLINE | ID: mdl-36592598

ABSTRACT

As a widely used industrial additive of plastic products, phthalate ester (PAE) plasticizers can easily migrate into food, threatening human health. In this work, we proposed a rapid, precise, and reliable method to detect PAE plasticizers in edible oils by using surface-enhanced Raman spectroscopy (SERS) technology. A two-dimensional (2D) silver plate synergizing with a nanosilver sol was prepared as a substrate for SERS to detect potassium hydrogen phthalate (PHP), a hydrolysate of a PAE plasticizer. Detection conditions, such as pH values, drying times, and hydrolysate interference, were optimized. The working curve was well fitted with a linear parameter R2 of 0.9994, and the minimum detection limit was evaluated as 10-9 mol/L. Furthermore, the detection accuracy was supported by five edible oil samples. Therefore, using SERS technology to detect PHP is expected to provide an avenue for PAE plasticizer detection in oils and fats, and it features promising potential applications in food safety.


Subject(s)
Metal Nanoparticles , Phthalic Acids , Humans , Plasticizers/chemistry , Spectrum Analysis, Raman/methods , Oils , Metal Nanoparticles/chemistry
3.
Comput Help People Spec Needs ; 13341: 253-260, 2022 Jul.
Article in English | MEDLINE | ID: mdl-36108327

ABSTRACT

Maps are indispensable for helping people learn about unfamiliar environments and plan trips. While tactile (2D) and 3D maps offer non-visual map access to people who are blind or visually impaired (BVI), this access is greatly enhanced by adding interactivity to the maps: when the user points at a feature of interest on the map, the name and other information about the feature is read aloud in audio. We explore how the use of an interactive 3D map of a playground, containing over seventy play structures and other features, affects spatial learning and cognition. Specifically, we perform experiments in which four blind participants answer questions about the map to evaluate their grasp of three types of spatial knowledge: landmark, route and survey. The results of these experiments demonstrate that participants are able to acquire this knowledge, most of which would be inaccessible without the interactivity of the map.

5.
Carbohydr Polym ; 277: 118853, 2022 Feb 01.
Article in English | MEDLINE | ID: mdl-34893262

ABSTRACT

For the purpose of developing multifunctional water purification materials capable of degrading organic pollutants while simultaneously inactivating microorganisms from contaminated wastewater streams, we report here a facile and eco-friendly method to immobilize molybdenum disulfide into bacterial cellulose via a one-step in-situ biosynthetic method. The resultant nanocomposite, termed BC/MoS2, was shown to possess a photocatalytic activity capable of generating •OH from H2O2, while also exhibiting photodynamic/photothermal mechanisms, the combination of which exhibits synergistic activity for the degradation of pollutants as well as for bacterial inactivation. In the presence of H2O2, the BC/MoS2 nanocomposite exhibited excellent antibacterial efficacy upwards of 99.9999% (6 log units) for the photoinactivation of both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus upon infrared (IR) lamp illumination (100 W, 760 nm ≤ λ ≤ 5000 nm, 15 cm vertical distance; 5 min). Mechanistic studies revealed synergistic pathogen inactivation resulting from the combination of photocatalytically generated •OH and hyperthermia induced by the photothermal conversion of the near-IR light. In addition, the BC/MoS2 nanocomposite also showed excellent photodegradation activity for common aqueous contaminants in the presence of H2O2, including malachite green (a textile dye), catechol violet (a phenol) and formaldehyde. Taken together, our findings demonstrate that sustainable materials such as BC/MoS2 have potential applications in wastewater treatment and microorganism disinfection.


Subject(s)
Anti-Bacterial Agents/pharmacology , Cellulose/pharmacology , Disulfides/pharmacology , Escherichia coli/drug effects , Molybdenum/pharmacology , Staphylococcus aureus/drug effects , Wastewater/microbiology , Anti-Bacterial Agents/chemistry , Cellulose/chemistry , Disulfides/chemistry , Microbial Sensitivity Tests , Molybdenum/chemistry , Water Purification
6.
Nanomaterials (Basel) ; 11(9)2021 Sep 08.
Article in English | MEDLINE | ID: mdl-34578648

ABSTRACT

Owing to the multi-dimensional complexity of human motions, traditional uniaxial strain sensors lack the accuracy in monitoring dynamic body motions working in different directions, thus multidirectional strain sensors with excellent electromechanical performance are urgently in need. Towards this goal, in this work, a stretchable biaxial strain sensor based on double elastic fabric (DEF) was developed by incorporating carboxylic multi-walled carbon nanotubes(c-MWCNTs) and polypyrrole (PPy) into fabric through simple, scalable soaking and adsorption-oxidizing methods. The fabricated DEF/c-MWCNTs/PPy strain sensor exhibited outstanding anisotropic strain sensing performance, including relatively high sensitivity with the maximum gauge factor (GF) of 5.2, good stretchability of over 80%, fast response time < 100 ms, favorable electromechanical stability, and durability for over 800 stretching-releasing cycles. Moreover, applications of DEF/c-MWCNTs/PPy strain sensor for wearable devices were also reported, which were used for detecting human subtle motions and dynamic large-scale motions. The unconventional applications of DEF/c-MWCNTs/PPy strain sensor were also demonstrated by monitoring complex multi-degrees-of-freedom synovial joint motions of human body, such as neck and shoulder movements, suggesting that such materials showed a great potential to be applied in wearable electronics and personal healthcare monitoring.

7.
ACS Appl Mater Interfaces ; 13(26): 31193-31205, 2021 Jul 07.
Article in English | MEDLINE | ID: mdl-34164984

ABSTRACT

Owing to the rise in prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics, infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts are an ever-increasing threat to public health. Thus, novel materials that can stem this crisis, while also functioning via multiple antimicrobial mechanisms so that pathogens are unable to develop resistance to them, are in urgent need. Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity. The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min). Mechanistic studies revealed that the use of cationic chitosan likely facilitated bacterial membrane disruption and/or permeability, with hyperthermia (photothermal) and reactive oxygen species (photodynamic) leading to synergistic pathogen inactivation upon visible-light illumination. No mammalian cell cytotoxicity was observed for the BC/MoS2-CS membrane, suggesting that such composite nanomaterials are attractive as functional materials for infection control applications.


Subject(s)
Anti-Bacterial Agents/pharmacology , Disulfides/pharmacology , Molybdenum/pharmacology , Nanocomposites/chemistry , Photosensitizing Agents/pharmacology , Animals , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/radiation effects , Anti-Bacterial Agents/toxicity , Cell Line , Cellulose/chemistry , Cellulose/toxicity , Chitosan/chemistry , Chitosan/toxicity , Disulfides/chemistry , Disulfides/radiation effects , Disulfides/toxicity , Escherichia coli/drug effects , Heating , Light , Membranes, Artificial , Mice , Microbial Sensitivity Tests , Molybdenum/chemistry , Molybdenum/radiation effects , Molybdenum/toxicity , Nanocomposites/radiation effects , Nanocomposites/toxicity , Photosensitizing Agents/chemistry , Photosensitizing Agents/radiation effects , Photosensitizing Agents/toxicity , Reactive Oxygen Species/metabolism , Staphylococcus aureus/drug effects
8.
Comput Help People Spec Needs ; 12376: 475-484, 2020 Sep.
Article in English | MEDLINE | ID: mdl-33225323

ABSTRACT

Augmented reality (AR) has great potential for blind users because it enables a range of applications that provide audio information about specific locations or directions in the user's environment. For instance, the CamIO ("Camera Input-Output") AR app makes physical objects (such as documents, maps, devices and 3D models) accessible to blind and visually impaired persons by providing real-time audio feedback in response to the location on an object that the user is touching (using an inexpensive stylus). An important feature needed by blind users of AR apps such as CamIO is a 3D spatial guidance feature that provides real-time audio feedback to help the user find a desired location on an object. We have devised a simple audio interface to provide verbal guidance towards a target of interest in 3D. The experiment we report with blind participants using this guidance interface demonstrates the feasibility of the approach and its benefit for helping users find locations of interest.

9.
J Technol Pers Disabil ; 8: 210-222, 2020.
Article in English | MEDLINE | ID: mdl-32802916

ABSTRACT

We describe a new approach to audio labeling of 3D objects such as appliances, 3D models and maps that enables a visually impaired person to audio label objects. Our approach to audio labeling is called CamIO, a smartphone app that issues audio labels when the user points to a hotspot (a location of interest on an object) with a handheld stylus viewed by the smartphone camera. The CamIO app allows a user to create a new hotspot location by pointing at the location with a second stylus and recording a personalized audio label for the hotspot. In contrast with other audio labeling approaches that require the object of interest to be constructed of special materials, 3D printed, or equipped with special sensors, CamIO works with virtually any rigid object and requires only a smartphone, a paper barcode pattern mounted to the object of interest, and two inexpensive styluses. Moreover, our approach allows a visually impaired user to create audio labels independently. We describe a co-design performed with six blind participants exploring how they label objects in their daily lives and a study with the participants demonstrating the feasibility of CamIO for providing accessible audio labeling.

10.
Anal Chim Acta ; 1119: 52-59, 2020 Jul 04.
Article in English | MEDLINE | ID: mdl-32439054

ABSTRACT

Tryptophan plays an important role in the metabolic pathway and tryptophan metabolism is abnormal in tumor tissues. Therefore, Trp and its metabolites in body fluids can be used as tumor markers for cancer monitoring. However, the traditional tryptophan detection method has many shortcomings, such as low sensitivity, time-consuming and so on, which is difficult to meet the clinical needs. Herein, a rapidly, sensitive and reliable method for indirectly detecting Tryptophan (Trp) in serum was proposed by combining diazo-coupling reaction mechanism with surface-enhanced Raman scattering (SERS). The limit of detection (LOD) of Trp can be as low as 20 nM, which is much lower than existing detection methods. In addition, because of obvious change in color in diazo-coupling reaction, a colorimetric detection was applied for convenient determination of Trp in the concentration ranged from 3 × 10-5 - 10-3 M. Furthermore, with the support of good results by SERS assay, the presented method was successfully carried out for the determination of Trp in serum that came from healthy people and colorectal cancer patients. Comparing the difference of Trp content in serum between the two groups, it was found that tryptophan metabolism disorder exists in colorectal cancer patients, which suggested that the accurate detection of tryptophan content may provide important reference for the pathogenesis, prediction and prevention of colorectal cancer.


Subject(s)
Colorimetry , Tryptophan/blood , Humans , Spectrum Analysis, Raman , Surface Properties
11.
Anal Chim Acta ; 1104: 199-206, 2020 Apr 01.
Article in English | MEDLINE | ID: mdl-32106952

ABSTRACT

We have successfully prepared a highly sensitive sandwich nanosensor combined Fe3O4 and Au@ATP@Ag nanorods for histamine detection based on surface-enhanced Raman spectroscopy (SERS). The Fe3O4 beads with -COOH served as a capture part to enrich histamine. The Au@ATP@Ag core-shell nanorods functionalized with Nalpha,Nalpha-Bis(carboxymethyl)-l-lysine (AB-NTA) were then used to connect with the imidazolyl group of histamine, simultaneously the internal standard 4-aminothiophenol (4-ATP) in the core-shell structure was used as the SERS signal. PLS regression model based on concentration range 10-3-10-8mol/L showed a linear trend with R2 = 0.9907. Our new approach can quickly and reliably determine histamine in fish sample and RAW264.7 cell lysates. This protocol for histamine extraction and SERS analysis enables the development of ultra-sensitive method for histamine detection.


Subject(s)
Adenosine Triphosphate/chemistry , Ferrosoferric Oxide/chemistry , Food Analysis/methods , Gold/chemistry , Histamine/analysis , Nanotubes/chemistry , Spectrum Analysis, Raman/methods , Animals , Fishes , Limit of Detection , Mice , Muscles/chemistry , RAW 264.7 Cells , Seafood/analysis , Surface Properties
12.
Biomed Opt Express ; 9(10): 4988-4997, 2018 Oct 01.
Article in English | MEDLINE | ID: mdl-30319916

ABSTRACT

Superhydrophobic silver films were fabricated by silver-mirror reaction and surface functionalization with thiol. The thiol-functionalization significantly improved the hydrophobic property of the Ag films (AFS), and their contact angle values slightly increased with the extension of a thiol alkyl chain, reaching about 160°. The surface-enhanced Raman scattering (SERS) detection capacity of these films were investigated, and AFS-Dodec showed the best substrate for R6G molecule detection with the concentration limit of 10-11 M. AFS functionalized with dodecanethiol (AFS-Dodec) was applied for the SERS detection of uric acid and creatinine, it exhibited good linear dependence relationship between the Raman intensity and analyte concentration in the concentration range of 5~1000 µM.

13.
Nanomaterials (Basel) ; 8(8)2018 Aug 20.
Article in English | MEDLINE | ID: mdl-30127278

ABSTRACT

Herein we utilized coordination interactions to prepare a novel core-shell plasmonic nanosensor for the detection of glucose. Specifically, Au nanoparticles (NPs) were strongly linked with Ag+ ions to form a sacrificial Ag shell by using 4-aminothiophenol (4-PATP) as a mediator, which served as an internal standard to decrease the influence of the surrounding on the detection. The resultant Au-PATP-Ag core-shell systems were characterized by UV-vis spectroscopy, transmission electron microscopy, and surface-enhanced Raman scattering (SERS) techniques. Experiments performed with R6G (rhodamine 6G) and CV (crystal violet) as Raman reporters demonstrated that the Au@Ag nanostructure amplified SERS signals obviously. Subsequently, the Au@Ag NPs were decorated with 4-mercaptophenylboronic acid (4-MPBA) to specifically recognize glucose by esterification, and a detection limit as low as 10-4 M was achieved. Notably, an enhanced linearity for the quantitative detection of glucose (R² = 0.995) was obtained after the normalization of the spectral peaks using 4-PATP as the internal standard. Finally, the practical applicability of the developed sensing platform was demonstrated by the detection of glucose in urine with acceptable specificity.

14.
Proc Conf Comput Robot Vis ; : 40-47, 2014 May.
Article in English | MEDLINE | ID: mdl-25210926

ABSTRACT

There is growing interest among smartphone users in the ability to determine their precise location in their environment for a variety of applications related to wayfinding, travel and shopping. While GPS provides valuable self-localization estimates, its accuracy is limited to approximately 10 meters in most urban locations. This paper focuses on the self-localization needs of blind or visually impaired travelers, who are faced with the challenge of negotiating street intersections. These travelers need more precise self-localization to help them align themselves properly to crosswalks, signal lights and other features such as walk light pushbuttons. We demonstrate a novel computer vision-based localization approach that is tailored to the street intersection domain. Unlike most work on computer vision-based localization techniques, which typically assume the presence of detailed, high-quality 3D models of urban environments, our technique harnesses the availability of simple, ubiquitous satellite imagery (e.g., Google Maps) to create simple maps of each intersection. Not only does this technique scale naturally to the great majority of street intersections in urban areas, but it has the added advantage of incorporating the specific metric information that blind or visually impaired travelers need, namely, the locations of intersection features such as crosswalks. Key to our approach is the integration of IMU (inertial measurement unit) information with geometric information obtained from image panorama stitchings. Finally, we evaluate the localization performance of our algorithm on a dataset of intersection panoramas, demonstrating the feasibility of our approach.

15.
Comput Help People Spec Needs ; 8547: 427-432, 2014.
Article in English | MEDLINE | ID: mdl-25599097

ABSTRACT

This paper describes recent progress on Crosswatch, a smartphone-based computer vision system developed by the authors for providing guidance to blind and visually impaired pedestrians at traffic intersections. One of Crosswatch's key capabilities is determining the user's location (with precision much better than what is obtainable by GPS) and orientation relative to the crosswalk markings in the intersection that he/she is currently standing at; this capability will be used to help him/her find important features in the intersection, such as walk lights, pushbuttons and crosswalks, and achieve proper alignment to these features. We report on two new contributions to Crosswatch: (a) experiments with a modified user interface, tested by blind volunteer participants, that makes it easier to acquire intersection images than with previous versions of Crosswatch; and (b) a demonstration of the system's ability to localize the user with precision better than what is obtainable by GPS, as well as an example of its ability to estimate the user's orientation.

16.
J Assist Technol ; 7(2)2013 Apr 01.
Article in English | MEDLINE | ID: mdl-24353745

ABSTRACT

PURPOSE: This paper describes recent progress on the "Crosswatch" project, a smartphone-based system developed for providing guidance to blind and visually impaired travelers at traffic intersections. Building on past work on Crosswatch functionality to help the user achieve proper alignment with the crosswalk and read the status of walk lights to know when it is time to cross, we outline the directions Crosswatch is now taking to help realize its potential for becoming a practical system: namely, augmenting computer vision with other information sources, including geographic information systems (GIS) and sensor data, and inferring the user's location much more precisely than is possible through GPS alone, to provide a much larger range of information about traffic intersections to the pedestrian. DESIGN/METHODOLOGY/APPROACH: The paper summarizes past progress on Crosswatch and describes details about the development of new Crosswatch functionalities. One such functionality, which is required for determination of the user's precise location, is studied in detail, including the design of a suitable user interface to support this functionality and preliminary tests of this interface with visually impaired volunteer subjects. FINDINGS: The results of the tests of the new Crosswatch functionality demonstrate that the functionality is feasible in that it is usable by visually impaired persons. RESEARCH LIMITATIONS/IMPLICATIONS: While the tests that were conducted of the new Crosswatch functionality are preliminary, the results of the tests have suggested several possible improvements, to be explored in the future. PRACTICAL IMPLICATIONS: The results described in this paper suggest that the necessary technologies used by the Crosswatch system are rapidly maturing, implying that the system has an excellent chance of becoming practical in the near future. ORIGINALITY/VALUE: The paper addresses an innovative solution to a key problem faced by blind and visually impaired travelers, which has the potential to greatly improve independent travel for these individuals.

17.
Proc IEEE Workshop Appl Comput Vis ; : 491-496, 2011 Jan 05.
Article in English | MEDLINE | ID: mdl-21804957

ABSTRACT

Modern household appliances, such as microwave ovens and DVD players, increasingly require users to read an LED or LCD display to operate them, posing a severe obstacle for persons with blindness or visual impairment. While OCR-enabled devices are emerging to address the related problem of reading text in printed documents, they are not designed to tackle the challenge of finding and reading characters in appliance displays. Any system for reading these characters must address the challenge of first locating the characters among substantial amounts of background clutter; moreover, poor contrast and the abundance of specular highlights on the display surface - which degrade the image in an unpredictable way as the camera is moved - motivate the need for a system that processes images at a few frames per second, rather than forcing the user to take several photos, each of which can take seconds to acquire and process, until one is readable.We describe a novel system that acquires video, detects and reads LED/LCD characters in real time, reading them aloud to the user with synthesized speech. The system has been implemented on both a desktop and a cell phone. Experimental results are reported on videos of display images, demonstrating the feasibility of the system.

18.
Proc IEEE Workshop Appl Comput Vis ; 2011(5-7 Jan. 2011): 503-510, 2011 Feb 10.
Article in English | MEDLINE | ID: mdl-21747737

ABSTRACT

There is a growing body of work addressing the problem of localizing printed text regions occurring in natural scenes, all of it focused on images in which the text to be localized is resolved clearly enough to be read by OCR. This paper introduces an alternative approach to text localization based on the fact that it is often useful to localize text that is identifiable as text but too blurry or small to be read, for two reasons. First, an image can be decimated and processed at a coarser resolution than usual, resulting in faster localization before OCR is performed (at full resolution, if needed). Second, in real-time applications such as a cell phone app to find and read text, text may initially be acquired from a lower-resolution video image in which it appears too small to be read; once the text's presence and location have been established, a higher-resolution image can be taken in order to resolve the text clearly enough to read it.We demonstrate proof of concept of this approach by describing a novel algorithm for binarizing the image and extracting candidate text features, called "blobs," and grouping and classifying the blobs into text and non-text categories. Experimental results are shown on a variety of images in which the text is resolved too poorly to be clearly read, but is still identifiable by our algorithm as text.

19.
Acta Crystallogr Sect E Struct Rep Online ; 66(Pt 9): m1129-30, 2010 Aug 18.
Article in English | MEDLINE | ID: mdl-21588531

ABSTRACT

The title dinuclear Fe(II) complex, [Fe(2)(SO(4))(2)(C(13)H(8)N(4))(2)(H(2)O)(4)]·2H(2)O, is centrosymmetric. Two sulfate anions bridge two Fe(II) cations to form the binuclear complex. Each Fe(II) cation is coordinated by two N atoms from a 1H-imidazo[4,5-f][1,10]phenanthroline (IP) ligand, two O atoms from two sulfate anions and two water mol-ecules in a distorted octa-hedral geometry. Extensive O-H⋯O, N-H⋯O and O-H⋯N hydrogen bonding is present in the crystal structure. Weak π-π stacking is observed between parallel IP ring systems, the face-to-face separation being 3.428 (14) Å.

20.
Comput Help People Spec Needs ; 6180: 229-234, 2010 Jul.
Article in English | MEDLINE | ID: mdl-26949756

ABSTRACT

Crossing an urban traffic intersection is one of the most dangerous activities of a blind or visually impaired person's travel. Building on past work by the authors on the issue of proper alignment with the crosswalk, this paper addresses the complementary issue of knowing when it is time to cross. We describe a prototype portable system that alerts the user in real time once the Walk light is illuminated. The system runs as a software application on an off-the-shelf Nokia N95 mobile phone, using computer vision algorithms to analyze video acquired by the built-in camera to determine in real time if a Walk light is currently visible. Once a Walk light is detected, an audio tone is sounded to alert the user. Experiments with a blind volunteer subject at urban traffic intersections demonstrate proof of concept of the system, which successfully alerted the subject when the Walk light appeared.

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