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1.
G3 (Bethesda) ; 10(1): 255-266, 2020 01 07.
Article in English | MEDLINE | ID: mdl-31719112

ABSTRACT

From yeast to humans, the cell cycle is tightly controlled by regulatory networks that regulate cell proliferation and can be monitored by dynamic visual markers in living cells. We have observed S phase progression by monitoring nuclear accumulation of the FHA-containing DNA binding protein Tos4, which is expressed in the G1/S phase transition. We use Tos4 localization to distinguish three classes of DNA replication mutants: those that arrest with an apparent 1C DNA content and accumulate Tos4 at the restrictive temperature; those that arrest with an apparent 2C DNA content, that do not accumulate Tos4; and those that proceed into mitosis despite a 1C DNA content, again without Tos4 accumulation. Our data indicate that Tos4 localization in these conditions is responsive to checkpoint kinases, with activation of the Cds1 checkpoint kinase promoting Tos4 retention in the nucleus, and activation of the Chk1 damage checkpoint promoting its turnover. Tos4 localization therefore allows us to monitor checkpoint-dependent activation that responds to replication failure in early vs. late S phase.


Subject(s)
S Phase Cell Cycle Checkpoints , Schizosaccharomyces pombe Proteins/metabolism , Transcription Factors/metabolism , Active Transport, Cell Nucleus , Cell Nucleus/metabolism , Checkpoint Kinase 1/genetics , Checkpoint Kinase 1/metabolism , DNA Replication , Mutation , Schizosaccharomyces , Schizosaccharomyces pombe Proteins/genetics , Transcription Factors/genetics
2.
J Vis Exp ; (148)2019 06 24.
Article in English | MEDLINE | ID: mdl-31282894

ABSTRACT

Live-cell imaging is a microscopy technique used to examine cell and protein dynamics in living cells. This imaging method is not toxic, generally does not interfere with cell physiology, and requires minimal experimental handling. The low levels of technical interference enable researchers to study cells across multiple cycles of mitosis and to observe meiosis from beginning to end. Using fluorescent tags such as Green Fluorescent Protein (GFP) and Red Fluorescent Protein (RFP), researchers can analyze different factors whose functions are important for processes like transcription, DNA replication, cohesion, and segregation. Coupled with data analysis using Fiji (a free, optimized ImageJ version), live-cell imaging offers various ways of assessing protein movement, localization, stability, and timing, as well as nuclear dynamics and chromosome segregation. However, as is the case with other microscopy methods, live-cell imaging is limited by the intrinsic properties of light, which put a limit to the resolution power at high magnifications, and is also sensitive to photobleaching or phototoxicity at high wavelength frequencies. However, with some care, investigators can bypass these physical limitations by carefully choosing the right conditions, strains, and fluorescent markers to allow for the appropriate visualization of mitotic and meiotic events.


Subject(s)
Cell Nucleus/metabolism , Meiosis , Microscopy, Fluorescence/methods , Mitosis , Schizosaccharomyces/cytology , Biomarkers/metabolism , Cell Cycle Proteins/metabolism , Chromosomal Proteins, Non-Histone/metabolism , Green Fluorescent Proteins/genetics , Schizosaccharomyces/genetics , Sepharose , Cohesins
3.
Genetics ; 212(2): 417-430, 2019 06.
Article in English | MEDLINE | ID: mdl-31000521

ABSTRACT

Fission yeast Swi6 is a human HP1 homolog that plays important roles in multiple cellular processes. In addition to its role in maintaining heterochromatin silencing, Swi6 is required for cohesin enrichment at the pericentromere. Loss of Swi6 leads to abnormal mitosis, including defects in the establishment of bioriented sister kinetochores and microtubule attachment. Swi6 interacts with Dfp1, a regulatory subunit of DBF4-dependent kinase (DDK), and failure to recruit Dfp1 to the pericentromere results in late DNA replication. Using the dfp1-3A mutant allele, which specifically disrupts Swi6-Dfp1 association, we investigated how interaction between Swi6 and Dfp1 affects chromosome dynamics. We find that disrupting the interaction between Swi6 and Dfp1 delays mitotic progression in a spindle assembly checkpoint-dependent manner. Artificially tethering Dfp1 back to the pericentromere is sufficient to restore normal spindle length and rescue segregation defects in swi6-deleted cells. However, Swi6 is necessary for centromeric localization of Rad21-GFP independent of DDK. Our data indicate that DDK contributes to mitotic chromosome segregation in pathways that partly overlap with, but can be separated from both, Swi6 and the other HP1 homolog, Chp2.


Subject(s)
Chromosomal Proteins, Non-Histone/metabolism , Chromosome Segregation/genetics , Protein Kinases/genetics , Protein Serine-Threonine Kinases/metabolism , Schizosaccharomyces pombe Proteins/genetics , Schizosaccharomyces pombe Proteins/metabolism , Schizosaccharomyces/genetics , Cell Cycle Proteins/metabolism , Centromere/metabolism , Chromobox Protein Homolog 5 , DNA Replication/genetics , Kinetochores/metabolism , Mitosis , Protein Kinases/metabolism , Repressor Proteins/metabolism , Schizosaccharomyces/metabolism , Cohesins
4.
Genes (Basel) ; 8(1)2017 Jan 18.
Article in English | MEDLINE | ID: mdl-28106789

ABSTRACT

The fission yeast centromere, which is similar to metazoan centromeres, contains highly repetitive pericentromere sequences that are assembled into heterochromatin. This is required for the recruitment of cohesin and proper chromosome segregation. Surprisingly, the pericentromere replicates early in the S phase. Loss of heterochromatin causes this domain to become very sensitive to replication fork defects, leading to gross chromosome rearrangements. This review examines the interplay between components of DNA replication, heterochromatin assembly, and cohesin dynamics that ensures maintenance of genome stability and proper chromosome segregation.

5.
Nucleic Acids Res ; 44(4): 1703-17, 2016 Feb 29.
Article in English | MEDLINE | ID: mdl-26682798

ABSTRACT

The formation of RNA-DNA hybrids, referred to as R-loops, can promote genome instability and cancer development. Yet the mechanisms by which R-loops compromise genome instability are poorly understood. Here, we establish roles for the evolutionarily conserved Nrl1 protein in pre-mRNA splicing regulation, R-loop suppression and in maintaining genome stability. nrl1Δ mutants exhibit endogenous DNA damage, are sensitive to exogenous DNA damage, and have defects in homologous recombination (HR) repair. Concomitantly, nrl1Δ cells display significant changes in gene expression, similar to those induced by DNA damage in wild-type cells. Further, we find that nrl1Δ cells accumulate high levels of R-loops, which co-localize with HR repair factors and require Rad51 and Rad52 for their formation. Together, our findings support a model in which R-loop accumulation and subsequent DNA damage sequesters HR factors, thereby compromising HR repair at endogenously or exogenously induced DNA damage sites, leading to genome instability.


Subject(s)
Alternative Splicing/genetics , Genomic Instability/genetics , Homologous Recombination/genetics , RNA Precursors/genetics , Schizosaccharomyces pombe Proteins/genetics , DNA/chemistry , DNA/genetics , DNA Repair/genetics , RNA/chemistry , RNA/genetics , Rad51 Recombinase/genetics , Rad52 DNA Repair and Recombination Protein/genetics , Schizosaccharomyces/genetics , Spliceosomes/genetics , Spliceosomes/metabolism
6.
Nucleus ; 5(4): 352-66, 2014.
Article in English | MEDLINE | ID: mdl-25482124

ABSTRACT

Appropriate targeting of inner nuclear membrane (INM) proteins is important for nuclear function and architecture. To gain new insights into the mechanism(s) for targeting and/or tethering peripherally associated proteins to the INM, we screened a collection of temperature sensitive S. cerevisiae yeast mutants for defects in INM location of the peripheral protein, Trm1-II-GFP. We uncovered numerous genes encoding components of the Spindle Pole Body (SPB), the yeast centrosome. SPB alterations affect the localization of both an integral (Heh2) and a peripheral INM protein (Trm1-II-GFP), but not a nucleoplasmic protein (Pus1). In wild-type cells Trm1-II-GFP is evenly distributed around the INM, but in SPB mutants, Trm1-II-GFP mislocalizes as a spot(s) near ER-nucleus junctions, perhaps its initial contact site with the nuclear envelope. Employing live cell imaging over time in a microfluidic perfusion system to study protein dynamics, we show that both Trm1-II-GFP INM targeting and maintenance depend upon the SPB. We propose a novel targeting and/or tethering model for a peripherally associated INM protein that combines mechanisms of both integral and soluble nuclear proteins, and describe a role of the SPB in nuclear envelope dynamics that affects this process.


Subject(s)
Nuclear Envelope/metabolism , Spindle Pole Bodies/metabolism , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae Proteins/metabolism
7.
PLoS Genet ; 10(3): e1004248, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24675878

ABSTRACT

The Never in Mitosis A (NIMA) kinase (the founding member of the Nek family of kinases) has been considered a mitotic specific kinase with nuclear restricted roles in the model fungus Aspergillus nidulans. By extending to A. nidulans the results of a synthetic lethal screen performed in Saccharomyces cerevisiae using the NIMA ortholog KIN3, we identified a conserved genetic interaction between nimA and genes encoding proteins of the Endosomal Sorting Complex Required for Transport (ESCRT) pathway. Absence of ESCRT pathway functions in combination with partial NIMA function causes enhanced cell growth defects, including an inability to maintain a single polarized dominant cell tip. These genetic insights suggest NIMA potentially has interphase functions in addition to its established mitotic functions at nuclei. We therefore generated endogenously GFP-tagged NIMA (NIMA-GFP) which was fully functional to follow its interphase locations using live cell spinning disc 4D confocal microscopy. During interphase some NIMA-GFP locates to the tips of rapidly growing cells and, when expressed ectopically, also locates to the tips of cytoplasmic microtubules, suggestive of non-nuclear interphase functions. In support of this, perturbation of NIMA function either by ectopic overexpression or through partial inactivation results in marked cell tip growth defects with excess NIMA-GFP promoting multiple growing cell tips. Ectopic NIMA-GFP was found to locate to the plus ends of microtubules in an EB1 dependent manner, while impairing NIMA function altered the dynamic localization of EB1 and the cytoplasmic microtubule network. Together, our genetic and cell biological analyses reveal novel non-nuclear interphase functions for NIMA involving microtubules and the ESCRT pathway for normal polarized fungal cell tip growth. These insights extend the roles of NIMA both spatially and temporally and indicate that this conserved protein kinase could help integrate cell cycle progression with polarized cell growth.


Subject(s)
Cell Cycle Proteins/genetics , Endosomal Sorting Complexes Required for Transport/genetics , Interphase/genetics , Microtubules/genetics , Protein Serine-Threonine Kinases/genetics , Aspergillus nidulans/genetics , Cell Cycle/genetics , Cell Nucleus/genetics , Endosomal Sorting Complexes Required for Transport/metabolism , Green Fluorescent Proteins , Mitosis/genetics , NIMA-Related Kinase 1
8.
Mol Biol Cell ; 25(6): 763-75, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24451264

ABSTRACT

Intercellular bridges are a conserved feature of multicellular organisms. In multicellular fungi, cells are connected directly via intercellular bridges called septal pores. Using Aspergillus nidulans, we demonstrate for the first time that septal pores are regulated to be opened during interphase but closed during mitosis. Septal pore-associated proteins display dynamic cell cycle-regulated locations at mature septa. Of importance, the mitotic NIMA kinase locates to forming septa and surprisingly then remains at septa throughout interphase. However, during mitosis, when NIMA transiently locates to nuclei to promote mitosis, its levels at septa drop. A model is proposed in which NIMA helps keep septal pores open during interphase and then closed when it is removed from them during mitosis. In support of this hypothesis, NIMA inactivation is shown to promote interphase septal pore closing. Because NIMA triggers nuclear pore complex opening during mitosis, our findings suggest that common cell cycle regulatory mechanisms might control septal pores and nuclear pores such that they are opened and closed out of phase to each other during cell cycle progression. The study provides insights into how and why cytoplasmically connected Aspergillus cells maintain mitotic autonomy.


Subject(s)
Cell Cycle Proteins/genetics , Fungal Proteins/genetics , Gene Expression Regulation, Fungal , Mitosis , Protein Serine-Threonine Kinases/genetics , Aspergillus nidulans/cytology , Aspergillus nidulans/genetics , Aspergillus nidulans/metabolism , Cell Cycle Proteins/metabolism , Cytoplasm/metabolism , Fungal Proteins/metabolism , Interphase/genetics , NIMA-Related Kinase 1 , Nuclear Pore/chemistry , Nuclear Pore/metabolism , Protein Serine-Threonine Kinases/metabolism , Signal Transduction
9.
Genetics ; 196(1): 177-95, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24214344

ABSTRACT

The nuclear pore complex proteins SonA and SonB, the orthologs of mammalian RAE1 and NUP98, respectively, were identified in Aspergillus nidulans as cold-sensitive suppressors of a temperature-sensitive allele of the essential mitotic NIMA kinase (nimA1). Subsequent analyses found that sonB1 mutants exhibit temperature-dependent DNA damage sensitivity. To understand this pathway further, we performed a genetic screen to isolate additional conditional DNA damage-sensitive suppressors of nimA1. We identified two new alleles of SonA and four intragenic nimA mutations that suppress the temperature sensitivity of the nimA1 mutant. In addition, we identified SonC, a previously unstudied binuclear zinc cluster protein involved with NIMA and the DNA damage response. Like sonA and sonB, sonC is an essential gene. SonC localizes to nuclei and partially disperses during mitosis. When the nucleolar organizer region (NOR) undergoes mitotic condensation and removal from the nucleolus, nuclear SonC and histone H1 localize in a mutually exclusive manner with H1 being removed from the NOR region and SonC being absent from the end of the chromosome beyond the NOR. This region of chromatin is adjacent to a cluster of nuclear pore complexes to which NIMA localizes last during its progression around the nuclear envelope during initiation of mitosis. The results genetically extend the NIMA regulatory system to include a protein with selective large-scale chromatin location observed during mitosis. The data suggest a model in which NIMA and SonC, its new chromatin-associated suppressor, might help to orchestrate global chromatin states during mitosis and the DNA damage response.


Subject(s)
Aspergillus nidulans/genetics , Cell Cycle Proteins/antagonists & inhibitors , Chromatin/genetics , DNA Repair/genetics , Protein Serine-Threonine Kinases/antagonists & inhibitors , Alleles , Amino Acid Sequence , Cell Cycle Proteins/genetics , Cell Nucleus/genetics , Chromosomes/genetics , DNA Damage/genetics , Fungal Proteins/genetics , Histones/genetics , Mitosis/genetics , Molecular Sequence Data , NIMA-Related Kinase 1 , Nuclear Envelope/genetics , Nuclear Pore/genetics , Nuclear Pore Complex Proteins/genetics , Nucleolus Organizer Region/genetics , Nucleoproteins/genetics , Protein Serine-Threonine Kinases/genetics
10.
Mol Biol Cell ; 24(24): 3842-56, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24152731

ABSTRACT

The NIMA kinase is required for mitotic nuclear pore complex disassembly and potentially controls other mitotic-specific events. To investigate this possibility, we imaged NIMA-green fluorescent protein (GFP) using four-dimensional spinning disk confocal microscopy. At mitosis NIMA-GFP locates to spindle pole bodies (SPBs), which contain Cdk1/cyclin B, followed by Aurora, TINA, and the BimC kinesin. NIMA promotes NPC disassembly in a spatially regulated manner starting near SPBs. NIMA is also required for TINA, a NIMA-interacting protein, to locate to SPBs during initiation of mitosis, and TINA is then necessary for locating NIMA back to SPBs during mitotic progression. To help expand the NIMA-TINA pathway, we affinity purified TINA and found it to uniquely copurify with An-WDR8, a WD40-domain protein conserved from humans to plants. Like TINA, An-WDR8 accumulates within nuclei during G2 but disperses from nuclei before locating to mitotic SPBs. Without An-WDR8, TINA levels are greatly reduced, whereas TINA is necessary for mitotic targeting of An-WDR8. Finally, we show that TINA is required to anchor mitotic microtubules to SPBs and, in combination with An-WDR8, for successful mitosis. The findings provide new insights into SPB targeting and indicate that the mitotic microtubule-anchoring system at SPBs involves WDR8 in complex with TINA.


Subject(s)
Aspergillus nidulans/genetics , Cell Cycle Proteins/genetics , Fungal Proteins/genetics , Mitosis/genetics , Protein Serine-Threonine Kinases/genetics , Amino Acid Sequence , Aurora Kinase A , CDC2 Protein Kinase/genetics , CDC2 Protein Kinase/metabolism , Cyclin B/genetics , Cyclin B/metabolism , Fungal Proteins/metabolism , G2 Phase/genetics , Green Fluorescent Proteins/genetics , Kinesins , Microscopy, Confocal , Mitosis Modulators/metabolism , Molecular Sequence Data , NIMA-Related Kinase 1 , Spindle Pole Bodies
11.
Eukaryot Cell ; 9(5): 831-3, 2010 May.
Article in English | MEDLINE | ID: mdl-20363899

ABSTRACT

A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. Examples defining the utility of the approaches, which should be widely applicable, are included.


Subject(s)
Chromatography, Affinity/methods , Fungal Proteins/isolation & purification , Proteomics/methods , Aspergillus nidulans/metabolism , Electrophoresis, Polyacrylamide Gel , Escherichia coli/metabolism , Saccharomyces cerevisiae/metabolism
12.
Mol Biol Cell ; 18(5): 1710-22, 2007 May.
Article in English | MEDLINE | ID: mdl-17332505

ABSTRACT

Podocalyxin/Gp135 was recently demonstrated to participate in the formation of a preapical complex to set up initial polarity in MDCK cells, a function presumably depending on the apical targeting of Gp135. We show that correct apical sorting of Gp135 depends on a bipartite signal composed of an extracellular O-glycosylation-rich region and the intracellular PDZ domain-binding motif. The function of this PDZ-binding motif could be substituted with a fusion construct of Gp135 with Ezrin-binding phosphoprotein 50 (EBP50). In accordance with this observation, EBP50 binds to newly synthesized Gp135 at the Golgi apparatus and facilitates oligomerization and sorting of Gp135 into a clustering complex. A defective connection between Gp135 and EBP50 or EBP50 knockdown results in a delayed exit from the detergent-resistant microdomain, failure of oligomerization, and basolateral missorting of Gp135. Furthermore, the basolaterally missorted EBP50-binding defective mutant of Gp135 was rapidly retrieved via a PKC-dependent mechanism. According to these findings, we propose a model by which a highly negative charged transmembrane protein could be packed into an apical sorting platform with the aid of its cytoplasmic partner EBP50.


Subject(s)
Sialoglycoproteins/metabolism , Amino Acid Motifs , Animals , Base Sequence , Binding Sites , Carrier Proteins/antagonists & inhibitors , Carrier Proteins/genetics , Cell Polarity , Cells, Cultured , Cytoskeletal Proteins/metabolism , Dogs , Endocytosis , Glycosylation , Membrane Microdomains/metabolism , Models, Biological , Mutation , Protein Kinase C/metabolism , Protein Sorting Signals/genetics , Protein Structure, Tertiary , RNA, Small Interfering/genetics , Sialoglycoproteins/chemistry , Sialoglycoproteins/genetics , Signal Transduction
13.
Histochem Cell Biol ; 127(4): 399-414, 2007 Apr.
Article in English | MEDLINE | ID: mdl-17180683

ABSTRACT

Podocalyxin (PC) was initially identified as a major sialoprotein on the apical surface of glomerular podocytes to perform the filtration barrier function. Later, it was reported to be expressed in endothelial cells, megakaryotes/platelets, and hemangioblasts, the common progenitor cells of the hematopoietic and endothelial cells. Recently, increasing numbers of reports have indicated that PC is not merely a molecule restricted at renal glomerulus, angiogenic or hematopoietic system. To further elucidate the expression pattern and address the possible physiological role of PC in adult mammals, we conducted an extensive study by immunohistochemistry and immunofluorescence staining on various tissues of healthy adult beagle dogs. By combinatory usage of two different anti-podocalyxin antibodies recognizing distinct epitopes in PC, we have demonstrated that (1) PC is expressed in renal tubules, mesothelium, myocardium, striated muscles in tongue, esophagus and extraocular region, myoepithelial cells in esophagus and salivary glands, neurons, and ependyma, etc.; (2) there are at least three forms of PC proteins, depending upon the accessibility of two different PC antibodies, expressed in different organs/systems; and (3) a particular form of PC is distributed in a vesicle-like compartment in certain organs/systems, such as the central nervous system.


Subject(s)
Biomarkers/analysis , Kidney Glomerulus/chemistry , Podocytes/chemistry , Sialoglycoproteins/analysis , Animals , Blotting, Western , Bone Marrow Cells/chemistry , Cell Line , Cell Line, Tumor , Digestive System/chemistry , Dogs , Endocrine System/chemistry , Eye/chemistry , Female , Genitalia, Female/chemistry , Genitalia, Male/chemistry , Humans , Immune System/chemistry , Immunohistochemistry , Kidney Glomerulus/cytology , Male , Myocardium/chemistry , Nervous System/chemistry , Podocytes/cytology , Urinary Tract/chemistry
14.
J Am Soc Nephrol ; 16(6): 1612-22, 2005 Jun.
Article in English | MEDLINE | ID: mdl-15814834

ABSTRACT

GP135 is an apical membrane protein expressed in polarized MDCK epithelial cells. When cultured in three-dimensional collagen gel, MDCK cells form branching tubules in response to hepatocyte growth factor stimulation in a manner that simulates the embryonic renal development. During this process, GP135 displays transient loss of membranous localization but reappears at the cell surface when nascent lumen emerges from the developing tubules. Despite being used for decades as the canonical hallmark of apical surface, the molecular identity and the significance of the dynamic expression of GP135 during the tubulogenic process remain elusive. For exploring the function of GP135, the full-length cDNA encoding GP135 was obtained. Sequence alignments and features analysis confirm GP135 as a canine homolog of podocalyxin, confirming the finding of an earlier independent study. Immunohistochemical assays on canine kidney sections identified both glomerular and tubular distribution of GP135 along the nephron. Mutant MDCK cells expressing siRNA targeted at two regions of GP135 show defects in hepatocyte growth factor-induced tubulogenesis. Re-expression of full-length and an O-linked glycosylation abbreviated construct of GP135 could recapitulate the tubulogenesis process lacking in siRNA knockdown cells; however, a deletion construct devoid of the cytoplasmic domain failed to rescue the phenotype. In summary, the data identify the MDCK apical domain marker GP135 as a tubular form of podocalyxin and provide evidence for its importance in renal tubulogenesis.


Subject(s)
Kidney Tubules/physiology , Sialoglycoproteins/physiology , Animals , Cell Line , Cloning, Molecular , DNA, Complementary , Dogs , Epithelial Cells , Membrane Glycoproteins/physiology , Mice
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