ABSTRACT
Increasing research has demonstrated that lncRNAs participate in the development of multiple cancer types. However, the role of TTNAS1 in endometrial cancer (EC) remains unknown. The present study aimed to explore the function of titinantisense RNA1 (TTNAS1) in EC progression and the underlying mechanisms. qRTPCR was performed to assess the TTNAS1 expression patterns in EC tissues and cell lines. Loss of function experiments were carried out to estimate the effects of TTNAS1 on EC cell proliferation, migration and invasion. To reveal the underlying mechanisms, informatics tools were used to predict the targets. Rescue experiments were performed to investigate the TTNAS1regulated miR376a3p/pumilio homolog 2 (PUM2) axis involved. The results of the present study revealed that TTNAS1 was highly expressed in both EC tissues and cell lines, and TTNAS1 knockdown inhibited EC cell proliferation, migration and invasion. With respect to the mechanisms, miR376a3p was revealed to be targeted by TTNAS1, and reversed the effects on EC development induced by TTNAS1. In addition, PUM2 was positively regulated by TTNAS1, and miR376a3p mediated the regulation between them. Furtherly, in vivo experiments confirmed the results. Collectively, TTNAS1 enhanced EC cell proliferation and metastasis by targeting the miR376a3p/PUM2 axis, which may shed light on EC diagnosis and treatment.
