ABSTRACT
OBJECTIVE: To study the chemical constituents of a marine-derived fungus possessing anti-tumor activity. METHODS: The compounds were isolated by various chromatographic methods and their structures were elucidated through spectroscopic analysis and chemical and physical properties. RESULTS: Nine compounds were isolated and identified as: (S)-(-)-N-benzoylphenylalaninol, asperphnamate, cerevisterol, (22E, 24R)-6beta-methnoxyergosta-7,22-diene-3beta, 5alpha-diol, (22E, 24R)-5alpha, 6alpha-epoxy-ergosta-8, 22-diene-3beta, 7alpha-diol, (22E, 24R) -3beta, 5alpha, 9alpha-trihydroxyergosta-7, 22-diene-6-one, (22E, 24R) -3beta-hydroxy-ergosta-5, 8, 22-trien-7-one, ergosterol, ergosterol peroxide. CONCLUSION: (22E, 24R)-3beta-hydroxy-ergosta-5, 8, 22-trien-7-one is isolated from microorganisms for the first time.
Subject(s)
Alkaloids/isolation & purification , Amides/isolation & purification , Antineoplastic Agents/isolation & purification , Ergosterol/isolation & purification , Fungi/chemistry , Alkaloids/chemistry , Amides/chemistry , Antineoplastic Agents/chemistry , Ergosterol/analogs & derivatives , Ergosterol/chemistry , Magnetic Resonance Spectroscopy , Marine Biology , Molecular Structure , Mycelium/chemistry , Phytosterols/chemistry , Phytosterols/isolation & purificationABSTRACT
Spectrophotometric determination of procaine hydrochloride is described. The procaine hydrochloride reacts with 1,2-naphthoquinone-4-sulfonic acid in pH 3.60 buffer solution to form a salmon pink compound, and its maximum absorption wavelength is at 484 nm, epsilon(484)=5.22 x 10(3).The absorbance for procaine hydrochloride from 0.30 to 100 microg ml(-1) obeys Beer's law. The linear regression equation of the calibration graph is C=19.23A-0.03, with a linear regression correlative coefficient is 0.9996, the detection limit is 0.28 microg ml(-1); recovery is from 98.0 to 105.2%. Effects of pH, surfactant, organic solvent, foreign ions, and standing time on the determination of procaine hydrochloride have been examined. This method is rapid and simple, and can be used for the determination of procaine hydrochloride in injection solution of procaine hydrochloride. The results obtained by this method agreed with those by the official method (dead-stop titration).
Subject(s)
Naphthoquinones , Pharmaceutical Preparations/chemistry , Procaine/analysis , Hydrogen-Ion Concentration , Indicators and Reagents , Solvents , Spectrophotometry/methodsABSTRACT
AIM: To study the chemical constituents in the mycelia of Hypomyces sp.. METHODS: Silica gel column chromatography was employed for the isolation and purification. Chemical and spectral methods were used to determine the structures of the isolated compounds. RESULTS: Two compounds were isolated and identified as: hypomycin C (I) and hypomycin D (II). CONCLUSION: Compounds I and II are new compounds.
Subject(s)
Hypocreales/chemistry , Perylene/isolation & purification , Quinones/isolation & purification , Chromatography, Gel/methods , Fermentation , Molecular Conformation , Molecular Structure , Mycelium/chemistry , Perylene/analogs & derivatives , Perylene/chemistry , Quinones/chemistryABSTRACT
OBJECTIVE: To study the chemical constituents of Shiraia bambusicola. METHOD: Column chromatography with silica gel was employed for the isolation and purification of constituents. The structures were elucidated by means of chemical and spectroscopic data. RESULT: Seven compounds were obtained and identified as hypocrellin A (I), hypocrellin B (II), hypocrellin C (III), hypomycin A (IV), ergosterol (V), ergosterol peroxide (VI) and 1,8-dihydroxy anthraquinone (VII). CONCLUSION: Compounds (IV) (VII) were separated from Shiraia bambusicola for the first time.
