ABSTRACT
BACKGROUND: Kinesio taping combined with manual lymphatic drainage for lower limb swelling and pain after total knee arthroplasty (TKA) has not been reported. The existing studies mainly concentrate on the instant effect of kinesio taping, and there is a lack of clinical research on its long-term effectiveness.OBJECTIVE: To observe the curative efficacy of kinesio taping combined with manual lymphatic drainage for lower limb swelling and pain after TKA.METHODS: Sixty patients with lower limb swelling following TKA were randomly divided into control and experimental groups (n=30 per group), followed by treated with kinesio taping, and kinesio taping combined with manual lymphatic drainage, respectively. The swelling degree, visual analogue scale scores and curative efficacy at baseline and 10 days after treatment were compared between two groups.RESULTS AND CONCLUSION: (1) The swelling degree and visual analogue scale scores were significantly decreased in the two groups after treatment (P < 0.05). Compared with the control group, all indicators were significantly improved in the experimental group (P < 0.05). (2) The total effective rate was 80% in the control group and 100% in the experimental group, and the obvious effective rate was 37% in the control group and 63% in the experimental group,both showing significant differences (P < 0.05). (3) These results suggest that the combination of kinesio taping and manual lymphatic drainage can markedly alleviate the low limb swelling and pain after TKA compared with single kinesio taping.
ABSTRACT
Calcium-binding protein is an indispensable protein which performs extensive and important functions in the growth of Schistosoma japonicum. Based on our primary study on tegument surface proteins of S. japonicun, a cDNA encoding a 66 kDa calcium-binding protein of S. japonicum (Chinese strain) was cloned, sequence analysis revealed that it was identical with that of SjIrV1 of Philippines strains S. japonicum. The expression of SjIrV1 were detected by Real-time PCR, using cDNA templates isolated from 7, 14, 21, 28, 35 and 42 days worms and the results revealed that the gene was expressed in all investigated stages, and the mRNA level of SjIrV1 is much higher in 42 d female worms than that in 42 d male worms. The cDNA containing the open reading frame of IrV1 was subcloned into a pET28a (+) vector and transformed into competent Escherichia coli BL21 for expression. The recombinant protein was purified using a Ni-NTA purification system, and confirmed by high performance liquid chromatography (RP-HPLC) and tandem mass spectrometry (MS/MS). Western blotting analysis showed that recombinant SjIrV1 (rSjIrV1) could be recognized by the S. japonicum infected mouse serum and the mouse serum specific to rSjIrV1, respectively. Immunofluorescence observation exhibited that SjIrV1 was mainly distributed on the tegument of the 35-day adult worms. ELISA test revealed that IgG, IgG1 and IgG2a antibodies are significantly increased in the serum of rSjIrV1 vaccinated mice. The study suggested that rSjIrV1 might play an important role in the development of S. japonicum.
