Post-blast histological changes to three animal bones exposed to close-range chemical detonation.

A range of investigative practices to aid explosive-related death investigations currently exist, although the use of histopathological bone samples to diagnose blast exposure and the distance of individuals from the blast source has not been previously reported. Forensic histopathology has been used effectively on soft tissue samples to define blast-related injuries effectively, analysing human organs such as the lungs, brain, liver, and skeletal muscles, providing important and useful forensic pathology interpretations. However, no studies currently exist examining the post-blast histological changes in human or animal bones subjected to blasts for forensic pathology practice, despite the opportunity that hard tissue bone samples present, given their significantly lower rate of decomposition over soft tissue. This study presents the first evidence-based findings on the post-blast histological changes in three animal bones when exposed to close-range chemical detonation (C4). The study's qualitative findings highlight critical changes in the tissue architecture of three different animal bone sources due to blast effects with range from the blast source. This emphasises the potential use of histopathological bone sample analysis in future blast-related death investigations, while providing ideas to further explore this work using larger-scale experiments and post-blast case studies in aid of applying this work to human samples and forensic pathology practice.

Insights into the primate trade into the European Union and the United Kingdom.

Illegal and/or unsustainable trade is a major obstacle to effective primate conservation. The wildlife trade in the European Union (EU) and the United Kingdom (UK) is significant, but for many species, such as primates, the trade is poorly understood and sparsely reported. All EU countries are Party to the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES); all primates are listed on Appendix I or II of CITES and are included on Annex A or B of Regulation (EC) No 338/97. We here combine data from several databases (CITES, UN Comtrade, TRAFFIC WiTIS) and seizure reports, to provide a narrative of the trade in primates into and within Europe. The legal import of live primates (2002-2021) amounted to 218,000-238,000 individuals (valued at US$ 869 million), with France, the UK, and Spain as the main importers and Mauritius, Vietnam, and China as the main exporters. Over 21,000 primate parts (trophies, skulls, bodies) were imported mainly from African countries, and UN Comtrade data suggests that ~ 600 tonnes of primate meat was imported mainly from Asia. The vast majority of live primates are either captive-born or captive-bred, and this proportion has increased over time. Reports of the illegal primate trade are far from complete, but the illegal trade of specific species or primate meat can have negative impacts of wild populations of already imperiled species. Stronger policies and more effective enforcement in consumer countries, such as the EU, would also aid in, and garner support for, better protecting primates in primate range states.

Determining the sustainability of legal wildlife trade.

Exploitation of wildlife represents one of the greatest threats to species survival according to the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services. Whilst detrimental impacts of illegal trade are well recognised, legal trade is often equated to being sustainable despite the lack of evidence or data in the majority of cases. We review the sustainability of wildlife trade, the adequacy of tools, safeguards, and frameworks to understand and regulate trade, and identify gaps in data that undermine our ability to truly understand the sustainability of trade. We provide 183 examples showing unsustainable trade in a broad range of taxonomic groups. In most cases, neither illegal nor legal trade are supported by rigorous evidence of sustainability, with the lack of data on export levels and population monitoring data precluding true assessments of species or population-level impacts. We propose a more precautionary approach to wildlife trade and monitoring that requires those who profit from trade to provide proof of sustainability. We then identify four core areas that must be strengthened to achieve this goal: (1) rigorous data collection and analyses of populations; (2) linking trade quotas to IUCN and international accords; (3) improved databases and compliance of trade; and (4) enhanced understanding of trade bans, market forces, and species substitutions. Enacting these core areas in regulatory frameworks, including CITES, is essential to the continued survival of many threatened species. There are no winners from unsustainable collection and trade: without sustainable management not only will species or populations become extinct, but communities dependent upon these species will lose livelihoods.

Ricochet of 9 mm pistol bullets on glazed ceramic tiles: An empirical study in support of shooting incident reconstruction.

Experimental ricochet data on the most commonly found bullet-target combinations in shooting incidents are contemporary necessity in conducting ricochet analysis during shooting reconstructions. As bullet ricochet behavior and associated evidence production are affected by various factors, understanding specific phenomena related to each bullet-target combination is essential for shooting investigators to reconstruct bullet ricochet incidents accurately. However, there is a significant lack of empirical data in this area despite the significant demand. Building on a previous study conducted with rifle bullets, this empirical study explores the ricochet behavior and surface evidence when 9 mm Luger pistol bullets ricochet off glazed ceramic wall and floor tiles. The study highlights the critical angles of bullets for floor and wall tiles (14.8 degrees and 16.6 degrees, respectively), and different bullet ricochet trends when 9 mm bullets ricochet off standard wall and floor tiles. This paper introduces for the first time in a ricochet event, the "caterpillar effect" or "caterpillar impact mark" and the first observation of the "nucleus" ricochet mark for pistol caliber bullet ricochets, both of which are important novel findings. This study further demonstrates the significance of undergoing practical and relevant ricochet experimentation for the accurate reconstruction of shooting incidents.

It's a sign: Animal welfare and zoo type are predictors of animal identification signage usage and quality at zoo exhibits.

Conservation education programs are listed as priority actions for almost every threatened species on the International Union for Conservation of Nature Red List. Zoos play an important role in delivering such programs, yet evidence of zoo education in many non-western countries is limited. Here, we evaluate animal identification signage prevalence and quality at zoo exhibits and investigate whether animal welfare, zoo type (accredited, government, and private), admission fee, zoo size, and proximity to urban centers are influencing factors. We used hornbills (Bucerotidae) as a model taxon, surveyed hornbill signage, and conducted welfare assessments of hornbill exhibits. We developed scoring frameworks and applied content analysis to analyze signage quality. Our results show that out of 18 zoos that displayed hornbills, 15 had hornbill signage. However, of the 106 hornbill exhibits in these zoos, 33% had no signage. We also found that signage presence or absence at individual zoos and signage quality is strongly correlated with animal welfare quality. Zoo type is a key factor in predicting signage and welfare quality, with accredited zoos scoring highest for both signage and welfare, followed by government and private zoos. Private zoos charged higher admission than other zoo types, and zoo size and proximity to urban centers did not influence signage or welfare scores. Overall, we conclude that in our study, signage usage and quality are inadequate, highlighting the importance of compliance with robust zoo standards to improve education and welfare within zoos to support global conservation goals.

An empirical study on the close-range post-ricochet orientation of AK bullets (7.62 mm × 39 mm).

Most of the injuries and deaths from ricocheting bullets in shooting incidents are usually reported due to misaimed shots that had ricocheted close to the victims. Although the destabilisation of ricocheted bullets during their ricochet flights is a generally known phenomenon, no significant quantitative-based scientific studies have attempted to understand bullets' post-ricochet orientations at close distances. This empirical study explores close-range post-ricochet orientations of AK bullets (7.62 mm × 39 mm) on a range of domestic surface types typically encountered during bullet ricochet incidents. This study has revealed that ricocheting AK bullets off of various wood types and tile samples produce side-on impacts into closely located targets following a rightwards yaw action. It has also been shown that AK bullets ricocheting off concrete and cement samples at 5-degree incident angles produced nose-forward impacts on paper witness screens, similar to an orthogonal impact of a direct-fired shot. The findings present important new information on the post-ricochet yawing behaviour of AK bullets, which has the potential to aid future shooting reconstructions in which victims are hit by closely ricocheted bullets.

The U.S. market for imported wildlife not listed in the CITES multilateral treaty.

The international wildlife trade presents severe conservation and environmental security risks, yet no international regulatory framework exists to monitor the trade of species not listed in the appendices of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). We explored the composition and dynamics of internationally regulated versus nonregulated trade, with a focus on importations of wild-caught terrestrial vertebrates entering the United States from 2009 to 2018. We used 10 years of species-level trade records of the numbers of live, wild-caught animals imported to the United States and data on International Union for the Conservation of Nature (IUCN) estimates of extinction risk to determine whether there were differences in the diversity, abundance, and risk to extinction among imports of CITES-listed versus unlisted species. We found 3.6 times the number of unlisted species in U.S. imports compared with CITES-listed species (1366 vs. 378 species). The CITES-listed species were more likely to face reported conservation threats relative to unlisted species (71.7% vs. 27.5%). However, 376 unlisted species faced conversation threats, 297 species had unknown population trends, and 139 species were without an evaluation by the IUCN. Unlisted species appearing for the first time in records were imported 5.5 times more often relative to CITES-listed species. Unlisted reptiles had the largest rate of entry, averaging 53 unique species appearing in imports for the first time per year. Overall trade quantities were approximately 11 times larger for imports of unlisted species relative to imports of CITES-listed species. Countries that were top exporters of CITES-listed species were mostly different from exporters of unlisted species. Because of the vulnerabilities of unlisted, traded species entering the United States and increasing global demand, we strongly recommend governments adapt their policies to monitor and report on the trade of all wildlife.

El Mercado Estadunidense de Fauna Importada No Enlistada en el Tratado Multilateral CITES Resumen Aunque el mercado internacional de fauna representa un riesgo severo para la conservación y la seguridad ambiental, no existe un marco internacional de regulación para monitorear el mercado de especies que no están en los apéndices de la Convención sobre el Comercio Internacional de Especies Amenazadas de Fauna y Flora Silvestres (CITES). Exploramos la composición y las dinámicas del mercado regulado internacionalmente frente al que no lo está, enfocados en la importación de vertebrados terrestres capturados en vida silvestre que entraron a Estados Unidos entre 2009 y 2018. Usamos el registro de comercio a nivel de especie del número de animales vivos capturados en vida silvestre e importados a Estados Unidos durante diez años y datos de los estimados de extinción de la Unión Internacional para la Conservación de la Naturaleza (UICN) para determinar si hay diferencias en la diversidad, abundancia y el riesgo de extinción entre las especies importadas enlistadas o no en CITES. Encontramos 3.6 veces más el número de especies no enlistadas en las importaciones a Estados Unidos en comparación con las especies enlistadas en CITES (1,366 versus 378 especies). Fue más probable que las especies de CITES enfrentaran amenazas de conservación reportadas en relación con las especies no enlistadas (71.7% vs. 27.5%). Sin embargo, 376 especies no enlistadas enfrentaron amenazas de conservación, 297 especies no cuentan con tendencias poblacionales conocidas y 139 especies no estaban evaluadas por la UICN. Las especies no enlistadas que aparecieron por primera vez en los registros fueron importadas 5.5 más veces en relación con las especies en CITES. La mayor tasa de entrada la tuvieron los reptiles no enlistados, con un promedio de 53 especies únicas al año registradas por primera vez en las importaciones. La cantidad generalizada de intercambios fue once veces mayor para la importación de especies no enlistadas en relación con la importación de especies CITES. La mayoría de los principales países exportadores de especies CITES fue diferente a la de los exportadores de especies no enlistadas. Debido a la vulnerabilidad de las especies comerciales no enlistadas que entran a los Estados Unidos y al incremento de la demanda global, recomendamos firmemente que los gobiernos adapten sus políticas para monitorear y reportar el mercado de toda la fauna.

Ricochet of AK bullets (7.62 MM X 39 MM) on glazed ceramic tiles: An empirical study in support of shooting incident reconstructions.

Indoor environments provide numerous hard surfaces and nearby objects which facilitate the shots fired to ricochet off and hit victims. Out of many surface types, ceramic tile surfaces are considered to be one of the most commonly available and encountered surface type in indoor shootings. However, no studies had attempted to understand the ricochet behaviour and surface evidence of ceramic tiles with any bullet type. This study explores the ricochet behaviour of one of the most commonly reported bullet type in recent shooting incidents; AK bullets (7.62 mm × 39 mm/ M43) on two glazed ceramic tile samples used for indoor walls and flooring. The study's results present the critical angles of glazed ceramic floor and wall tile samples along with a few significant and currently not reported ricochet-surface mark characteristics with greater forensic significance for use in AK gun-related ricochet investigations. This study further emphasises the need for case-by-case empirical approaches to understanding the ricochet behaviour of different bullet-target combinations during ricochet investigations. The study also opens up a new research area to explore whether the observed results are common to steel core AK bullets or common to other ammunition types and tile surfaces with different compositions.

Dataset of seized wildlife and their intended uses.

The illegal wildlife trade (IWT) threatens conservation and biosecurity efforts. The Internet has greatly facilitated the trade of wildlife, and researchers have increasingly examined the Internet to uncover illegal trade. However, most efforts to locate illegal trade on the Internet are targeted to one or few taxa or products. Large-scale efforts to find illegal wildlife on the Internet (e-commerce, social media, dark web) may be facilitated by a systematic compilation of illegally traded wildlife taxa and their uses. Here, we provide such a dataset. We used seizure records from three global wildlife trade databases to compile the identity of seized taxa along with their intended usage (i.e., use-type). Our dataset includes c. 4.9k distinct taxa representing c. 3.3k species and contains c. 11k taxa-use combinations from 110 unique use-types. Further, we acquired over 45k common names for seized taxa from over 100 languages. Our dataset can be used to conduct large-scale broad searches of the Internet to find illegally traded wildlife. Further, our dataset can be filtered for more targeted searches of specific taxa or derived products.

Ricochet of AK bullets (7,62 × 39 mm) on concrete and cement surfaces; a forensic-based study.

The ricochet behaviour of AK bullets (7,62 x 39 mm) on two different concrete samples (rough and a cement-skimmed intermediate) and one cement sample were explored in this study. The estimated critical angles for these surfaces were shown to be 10.8° and 11.1° for the rough and intermediate concrete surfaces and 13.2° for the cement surfaces. In all occasions, fragmentation of the bullets was observed upon reaching critical angles. The results from the concrete surfaces highlighted the sensitivity of AK bullet ricochet angles from concrete surfaces with a different surface roughness and composition. Almost all resulting ricochet angles for the more frangible cement surfaces were observed to be much higher than currently reported literature would suggest. Consistent bullet wipe markings were observed on most samples, acting as a strong indicator for the directionality of the projectile during ricochet. Interestingly for the cement surface, a clear relationship was reported between the angle of incidence and the depth of the crater produced. Ultimately, this study highlights the potential uncertainties and possible errors that could occur in bullet trajectory determination from ricochet marks for this bullet and target combination if theoretic interpretations alone are used. There is no substitute for bullet and surfacespecific practical trials if the most accurate shooting scene reconstructions are to be carried out.

AK bullet (7.62 × 39 mm) holes on 1-mm sheet metal: A forensic-related study in aid of bullet trajectory reconstruction.

Bullet holes play a major role in the trajectory determination of fired bullets, potentially suggesting where the shots were fired from. Few methods are currently employed to estimate the angles of incidence of fired bullets using bullet holes. The literature has stressed that knowledge about the impact behavior of the specific bullet-substrate interfaces, including the most viable method for estimating the angle of incidence of that combination, requires empirical testing to enable successful analysis. The perforating bullet hole characteristics of AK bullets fired into 1-mm sheet metal over a range of angles of incidence have been documented in this study to aid bullet trajectory determination. A strong inverse relationship has been demonstrated between particular impact mark dimensions and the angles of incidence, revealing the possibility of using these dimensions to predict the angles of incidence for AK bullets perforating 1-mm sheet metal in actual crime scenes. This study has further confirmed the existence of a recently reported phenomenon for impacts related to this projectile-substrate combination, the "double-headed impact mark," and relationships with the angles of incidence of fired AK bullets. While suggesting an alternative and confirmative new method to understand the angles of incidence of fired AK bullets in 1-mm sheet metal using measured bullet defects, the study highlights a complex deviation phenomenon and a potential error that could occur when determining bullet trajectories using probing, stringing, or laser methods for the impact conditions described.

AK bullet (7,62 × 39 mm) ricochet off flat, wooden targets; A forensic-based study.

The ricochet behaviour of AK bullets (7,62 × 39 mm), one of the prominently reported bullet type in recent shooting incidents worldwide, has been examined on a range of different wooden surfaces in this study. The critical angles of AK bullets for teak, Jack wood, mahogany and pine were reported with close values ranging from 9.9 to 13.3- degrees, suggestive of being a valuable reference that can be used during AK bullet ricochet-related shooting investigations with similar conditions. The study also highlighted a significant phenomenon against the existing understanding on the wood hardness and the critical angles of bullets. The widths of the AK bullet wipe marks generated on the wood surfaces were highly consistent, regardless of impact angle, allowing an effective approximation of bullet caliber. Bullet tunnelling effect were also observed with pinewood samples in this study. The study also further highlights the great variability of bullet ricochet phenomena and the challenging nature of using the theoretical and experimental results relates to wood ricochet studies for shooting reconstructions.

A standardized dataset for conservation prioritization of songbirds to support CITES.

In this article we present a standardized dataset on 6659 songbirds (Passeriformes) highlighting information relevant to species conservation prioritization with a main focus to support the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). Data were collected from both scientific and grey literature as well as several online databases. The data are structured into six knowledge categories: Conventions and Treaties, Human Use, Extinction Risk, Management Opportunities, Biological Information, and Intrinsic Values. The Conventions and Treaties category includes the listings for two international conventions, CITES and the Convention on the Conservation of Migratory Species of Wild Animals (CMS), as well as EU listings for the EU Wildlife Trade Regulations and the EU Birds Directive. The Human Use category contains information on both regulated trade collected from the CITES Trade Database and the United States' Law Enforcement Management Information System (LEMIS), and highly aggregated data on seizures which we obtained from TRAFFIC, the United Nations Office on Drugs and Crime (UNODC) and two data sources on traditional medicine. We also present, for the first time, the complete Songbirds in Trade Database (SiTDB), a trade database curated by taxon expert S. Bruslund based on expert knowledge, literature review, market surveys and sale announcements. Data on the types of human use, including traditional medicine are also provided. The knowledge area on Extinction Risk contains data on the species' IUCN Red List status, the Alliance for Zero Extinction Trigger Species status, site and population at the site, the species' IUCN Climate Change Vulnerability Assessment, and the listing of priority species at the Asian Songbird Crisis Summit. In the Management Opportunities category, we gathered data on ex-situ management from Species360 zoo holdings as well as species management plans from the European and North American Zoo Associations (EAZA and AZA, respectively). Biological Information includes data on body mass, clutch size, diet, availability of data from the IUCN Red List on habitat systems, extent of occurrence, generation length, migration pattern, distribution, and biological data from the Demographic Species Knowledge Index, number of occurrences recorded by the Global Biodiversity Information Facility (GBIF) as well as genomic data from the Bird 10 000K Genomes (B10K) project, Vertebrate Genome Project (VGP) and GenBank. Information on invasive species is also part of this knowledge area. The Intrinsic Value category refers to two measures of the species' intrinsic value, namely Ecological and Evolutionary Distinctiveness. In order to make these knowledge areas comparable, we standardized data following the taxonomy of the Handbook of the Birds of the World and Birdlife (Version 4, 2019). The data enable a broad spectrum of analyses and will be useful to scientists for further research and to policymakers, zoos and other conservation stakeholders for future prioritization decisions.

A forensic-based study on low angled AK rifle bullet entry wounds using a porcine model.

An entry wound normally carries more macroscopic evidence than found in exit wounds. In addition to common wound characteristics of an entry wound such as an abrasion collar, burn marks etc., large amounts of trace evidences from the bullet and gun such as gunshot residues, powder tattoo, grease ring (bullet wipe) etc., are also present in entry wounds making them a richer source of evidence than exit wounds. This forensic-based study explores the low-angled entry wound characteristics of AK rifle bullets (7,62 mm × 39 mm) on porcine skin. This demonstrated a special entry wound characteristic similar to the commonly reported "abrasion ring" but differs in structure with an abraded area around the entry wounds and more significantly damaged outer margins. A significant inverse relationship between the AK bullet's approaching angle and the length of the external entry wound has also been reported in this work, with each finding contributing new knowledge to the forensic pathology of gunshot wounds. The findings of this study also have the potential to aid in shooting incident reconstructions.

Forensic based empirical study on ricochet behaviour of Kalashnikov bullets (7.62mm×39mm) on 1mm sheet metal.

Bullet ricochet off a surface in a shooting scene occurs in diverse conditions and affected by array of factors. Therefore ricochet analysis of a particular incident demands case by case analysis supported by the knowledge of existing subject knowledge and empirical testing. In this view, existing empirical test results on bullet ricochet experiments have become always assisted and referred by investigators during scene reconstruction and in Courts. This forensic based research was aimed to understand the ricochet behaviour and related aspects of Kalashnikov bullets (7.62mm×39mm) on 1mm sheet metal. 1mm sheet metal was selected as the target surface of the study, based on its greater possibility of existing in concurrent urban environments as vehicle bodies, electrical appliances, road signs, boundary walls, partitions, walls of mobile houses etc. The research added brand new knowledge to the firearm investigation field in general and specifically to AK shooting investigations and scene reconstruction. The ricochet angles and critical angle of 1mm sheet metal and AK bullets, relationship of different impact feathers and angle of incidences are some of the main findings of the research. The double head impact mark which produces as a result of bullet's interaction with the target and it's specific relationships with the incident angles is the other most important results of the research which has not reported so far in a bullet ricochet study. In addition to the contribution of knowledge to the field of forensic sciences in general and forensic ballistic in particular, this research further emphasises the need for case by case empirical study to understand the ricochet behaviour of different bullet and target combinations. On the other hand, this study very deeply convinced firearms investigators on the risk of applying general bullet ricochet theory on yielding and nonyielding surfaces to reconstruct all bullet ricochet incidents which would result in wrong interpretations, critical errors in shooting scene reconstruction and finally for false testimonies.

Effect of PET Image Reconstruction Techniques on Unexpected Aorta Uptake

Objectives: To determine if unexpected aorta uptake seen in some patients is influenced by popular modern reconstruction algorithms using semi-quantitative and qualitative analysis. Methods: Twenty-five consecutive patients without suspected vascular disease were selected for 18F-FDG positron emission tomography/ computed tomography (PET/CT) scanning and images of the aorta were created using iterative reconstruction (IT), IT + time of flight (TOF), IT + TOF + point spread function correction (referred collectively as UHD) with and without metal artefact reduction (MAR) algorithms. An experienced radiologist created aorta and blood pool (BP) regions of interests then copied these to all reconstructions for accurate positioning before recording target aorta standardized-uptake-values (SUVmax) and background BP SUVmean. Furthermore, target-to-background ratio (TBRmax) was defined by aorta SUVmax-to-BP SUVmean ratio for more analysis. Results: For aorta SUVmax with IT, IT + TOF, UHD, UHD + MAR reconstructions the mean ± standard deviation recorded were 2.15±0.43, 2.25±0.51, 2.25±0.45 and 2.09±0.4, respectively. Values for BP SUVmean were 1.61±0.31, 1.58±0.28, 1.58±0.28 and 1.47±0.25, respectively. Likewise, for TBRmax these were 1.35±0.19, 1.43±0.21, 1.43±0.19, 1.43±0.18, respectively. ANOVA analysis revealed no significant differences for aorta SUVmax (F(0.86) p=0.46), BP SUVmean (F(1.22) p=0.31) or TBRmax (F(0.99) p=0.4). However, the qualitative visual analysis revealed significant differences between IT + TOF with UHD (p=0.02) or UHD + MAR (p=0.02). Conclusion: Reconstruction algorithm effect on aorta SUVmax or BP SUVmean or TBRmax was not statistically significant. However, qualitative visual analysis showed significant differences between IT + TOF as compared with UHD or UHD + MAR reconstructions. Harmonization of techniques with a larger patient cohort is recommended in future clinical trials.

Ethnozoological assessment of animals used by Mon traditional medicine vendors at Kyaiktiyo, Myanmar.

ETHNOPHARMACOLOGICAL RELEVANCE: Wild animals are widely used in traditional Asian medicine but information from Myanmar is lacking. We show that a wide range of animals are used at a pilgrimage site, mostly for their rendered fats and oils to be used in mixed concoctions. The majority of species were sold to be used to treat aching joints, muscle ache and skin diseases. AIM OF THE STUDY: To assess wildlife for sale for medicinal purposes, and document their medicinal use at Kyaiktiyo, a pilgrimage site at a 1100m tall mountain, with many of the pilgrims climbing to the top. In addition we address legal issues relating to the production and sale of traditional medicine that contain legally protected animals. MATERIAL AND METHODS: Four visits were made to Kyaiktiyo, Myanmar, between 2000 and 2017 to quantify animal parts on display and through discussions with vendors to obtain information on medicinal use of these parts. RESULTS: Twenty-three species, mostly mammals, were recorded to be used for traditional medicine. The most common were Chinese serow Capricornis milneedwardsii, Asian elephant Elephas maximus, and Asiatic black bear Ursus thibetanus. Over 600 bodies or body parts were present. Combined, these parts purportedly provided cures or relief for at least 15 ailments or diseases. The most commonly mentioned treatment was that of using rendered animal fats/oils externally to relieve/cure aching joints or muscles. This treatment allegedly provides instant relief to pilgrims after an arduous climb up the mountain. Purported cures for various skin diseases was the next common use for the animal species on offer. Ten of the species observed for sale at Kyaiktiyo are listed as globally threatened, and 15 are protected and cannot be legally traded. Ambiguities in Myanmar's legislation mean that protected animals or their body parts cannot be traded, however traditional medicines can be made out of them provided rules relating to the manufacturing of traditional medicines are adhered to. CONCLUSION: This study indicated that animals and their parts continue to be openly offered for sale at Kyaiktiyo to treat various illnesses. Despite these products potential medical, traditional or cultural importance, solutions have to been found on how to ensure that, in line with Myanmar's laws, use of traditional local medicine does not impede the conservation of imperilled species.

MicroRNA and gene co-expression networks characterize biological and clinical behavior of rhabdomyosarcomas.

Rhabdomyosarcomas (RMS) in children and adolescents are heterogeneous sarcomas broadly defined by skeletal muscle features and the presence/absence of PAX3/7-FOXO1 fusion genes. MicroRNAs are small non-coding RNAs that regulate gene expression in a cell context specific manner. Sequencing analyses of microRNAs in 64 RMS revealed expression patterns separating skeletal muscle, fusion gene positive and negative RMS. Integration with parallel gene expression data assigned biological functions to 12 co-expression networks/modules that reassuringly included myogenic roles strongly correlated with microRNAs known in myogenesis and RMS development. Modules also correlated with clinical outcome and fusion status. Regulation of microRNAs by the fusion protein was demonstrated after PAX3-FOXO1 reduction, exemplified by miR-9-5p. MiR-9-5p levels correlated with poor outcome, even within fusion gene positive RMS, and were higher in metastatic versus non-metastatic disease. MiR-9-5p reduction inhibited RMS cell migration. Our findings reveal microRNAs in a regulatory framework of biological and clinical significance in RMS.