ABSTRACT
The specific oxidase activity of ceruloplasmin (activity per unit mass of enzyme protein) was studied in plasma of pregnant women with gestosis, feto-placental insufficiency, postmature and in normal pregnancy. The specific oxidase activity decreased in all groups with pathology. The ceruloplasmin concentration decreased in the postmature group.
Subject(s)
Ceruloplasmin/analysis , Oxidoreductases/blood , Placental Insufficiency/blood , Pre-Eclampsia/blood , Adult , Ceruloplasmin/metabolism , Copper/blood , Female , Humans , Infant, Newborn , Infant, Postmature/blood , Pregnancy/bloodABSTRACT
Oxidase activity dynamics in the blood plasma of test animals was studied upon intravenous and intramuscular injections of ceruloplasmin. It was found that the enzymatic activity does not reflect the content of drug in the blood plasma and is not always correlated with the amount of exogenously introduced drug. Intramuscular injections can also be used for the administration of ceruloplasmin.
Subject(s)
Ceruloplasmin/pharmacokinetics , Animals , Female , Injections, Intramuscular , Injections, Intravenous , Kinetics , Male , RatsABSTRACT
The immunochemically pure preparation of lactoferrin was isolated from human colostrum and used for the immunization of animals with a view of obtaining antiserum, and also as a reference preparation for the determination of the content of lactoferrin in the standard. The monospecific antiserum to lactoferrin, obtained as the result of this procedure, was used for the determination of the content of lactoferrin in samples of human milk by the method of radial immunodiffusion. Through the content of lactoferrin in human milk showed considerable fluctuations, its level essentially decreased on the second week of lactation. In cases of the microbial contamination of milk the tendency towards an increase in the content of lactoferrin was observed irrespective of the time of lactation.
