ABSTRACT
The completion of the Arabidopsis thaliana genome sequence allows a comparative analysis of transcriptional regulators across the three eukaryotic kingdoms. Arabidopsis dedicates over 5% of its genome to code for more than 1500 transcription factors, about 45% of which are from families specific to plants. Arabidopsis transcription factors that belong to families common to all eukaryotes do not share significant similarity with those of the other kingdoms beyond the conserved DNA binding domains, many of which have been arranged in combinations specific to each lineage. The genome-wide comparison reveals the evolutionary generation of diversity in the regulation of transcription.
Subject(s)
Arabidopsis/genetics , Caenorhabditis elegans/genetics , Drosophila melanogaster/genetics , Genome , Saccharomyces cerevisiae/genetics , Transcription Factors/genetics , Amino Acid Motifs , Animals , Arabidopsis/chemistry , Caenorhabditis elegans/chemistry , DNA/metabolism , Drosophila melanogaster/chemistry , Eukaryotic Cells , Evolution, Molecular , Gene Duplication , Genome, Plant , Protein Binding , Protein Structure, Tertiary , Saccharomyces cerevisiae/chemistry , Transcription Factors/chemistry , Transcription Factors/metabolismABSTRACT
A consensus map for loblolly pine (Pinus taeda L.) was constructed from the integration of linkage data from two unrelated three-generation outbred pedigrees. The progeny segregation data from restriction fragment length polymorphism, random amplified polymorphic DNA, and isozyme genetic markers from each pedigree were recoded to reflect the two independent populations of parental meioses, and genetic maps were constructed to represent each parent. The rate of meiotic recombination was significantly greater for males than females, as was the average estimate of genome length for males (1983.7 cM [Kosambi mapping function (K)]) and females [1339.5 cM(K)]. The integration of individual maps allows for the synthesis of genetic information from independent sources onto a single consensus map and facilitates the consolidation of linkage groups to represent the chromosomes n = 12 of loblolly pine. The resulting consensus map consists of 357 unique molecular markers and covers approximately 1300 cM(K).
