Effect of acute progestational hypoxia on the content of biogenic amines in the brain of albino rat pups: Peptide correction.

We studied the effect of exposure to acute hypobaric hypoxia in the progestational period on the content of biogenic amines in the brainstem and cerebral cortex in rat pups of different age. The possibility of correcting hypoxia-induced changes with regulatory peptides was evaluated. We found that early antenatal hypoxia disturbs maturation of catecholaminergic systems in the brain. It should be emphasized that the differences from the control varied depending on the age of rat pups. Single intranasal administration of Semax heptapeptides and beta-casomorphine-7 to pregnant females prevented changes in the content of biogenic amines in CNS of the offspring during postnatal ontogeny.

[Effectiveness of dietary non-starch polysaccharides in experimental toxic hepatitis]. / Effektivnost' pishchevykh nekrakhmal'nykh polisakharidov pri éksperimental'nom toksicheskom gepatite.

The efficiency of some polysaccharides was investigated in mice with an experimental toxic hepatitis. Hepatitis was induced by the oral administration of 10% solution CCl4 in olive oil at a dosage of 3 ml/kg body weight every day during 7 days. After that tested substances were administrated every day 30-40 min before a feeding at a dosage of 150 mg/kg body weight during 14-21 days. Results showed that a calcium alginate, two low-methoxyl pectins (one with the degree of esterification about 50% and other with the degree of esterification less 5%), fucoidan, and chitozan, but not lambda-carrageenan and kappa-carrageenan, have beneficial affects on liver total lipid, glycogen, malondialdehyde, and diene conjugates as well as on blood total lipid and alanine aminotransferase activity in animals with experimental toxic hepatitis.

[The biogenic amine content of rat tissues in the postresuscitation period following hemorrhagic shock and the effect of the preparation semax]. / Soderzhanie biogennykh aminov v tkaniakh krys v postreanimatsionnom periode posle gemorragicheskogo shoka i vliianie preparata semaks.

LPO products were measured in plasma and biogenic amines (serotonin, adrenalin, noradrenalin) in tissues of rats in different periods after hemorrhagic shock provoked by taking blood and maintenance of arterial pressure at the level of 40 mm Hg for 1 hour. Resuscitation was conducted by administration of autoblood. It was found that splenic serotonin levels decreased on experiment day 7 and went up on day 28. On late experiment stages noradrenalin levels in the adrenals were high. Early after resuscitation the trend was noted to higher LPO products concentration in plasma and serotonin in the brain stem. Intravenous injection of semax prevented serotonin fall in the spleen on experiment day 7. It is suggested that biogenic amines, especially serotonin system, are involved in mechanisms of postresuscitation disorders, in cerebral defects in particular, through prolongation of secondary hypoxia early after hemorrhagic shock and activation of hypothalamo-hypophyso-adrenal system late after the shock.

[The action of sublethal doses of ionizing radiation on the endogenous radioresistance background]. / Issledovanie deistviia subletal'nykh doz ioniziruiushchego izlucheniia na éndogennyi fon radiorezistentnosti.

Sublethal doses of X radiation (0.5 Gy and 1 Gy) caused the alterations in levels of main components of endogenous radioresistance background in rat tissues. There were demonstrated the decrease of serotonin content in stomach mucosa and spleen, adrenalin, noradrenalin and corticosteroid contents in adrenal glands, nonprotein thiols content in spleen, and the increase of lipid peroxide level in serum on the 3-14 days after irradiation. The recovery of the investigated parameters was occurred to the 21 day after exposure.

[Effects of MIGI-K on general resistance of the body]. / Vliianie MIGI-K na obshchuiu rezistentnost' organizma.

The data on the effect of MIGI-K preparation, acidic hydrolyzate from mussel meat, on organism resistance are presented. The effect is based on the ability of MIGI-K to influence the level of endogenous compounds responsible for radioresistance, such as biogenic amines and lipid hydroperoxides, as well as certain parameters determining immune status of organism.

[Electrophoretic separation of rat spleen and thymus leukocytes and their reactions to mitogens in vitro]. / Elektroforeticheskoe razdelenie limfotsitov selezenki i timusa krys i ikh reaktsiia na mitogeny in vitro.

Electrophoretic mobility (EPM) of lymphocytes from the thymus and spleen of August and Wistar rats as well as capacity of lymphocytes with different surface hemagglutinin (PHA) and concanavalin A (Con A) were studied by the method of free flow electrophoresis. Lymphocytes of the rat spleen were shown, depending on the surface charge, to divide into two groups during cultivation: cells with high and low electrophoretic mobility. At separation the lymphocytes consisted of 8--10 fractions with different EPM. There was a relationship between the surface charge of the lymphocytes and their stimulation rate by mitogens. Increased thymidine-3H uptake was recorded at mitogenic exposure of lymphocytes from the spleen with high EPM. Low mobile lymphoid elements of the spleen did not respond to mitogenic stimulation. A subpopulation of thymocytes with low EPM was resistant to Con A stimulation. The thymocytes of rats did not virtually respond to PHA irrespective of EPM.

[Interaction of mitogenic lectins with lymphocyte plasma membranes]. / Vzaimodeistvie mitogennykh lektinov s plazmaticheskimi membranami limfotsitov.

It is shown that mitogenic stimuli from concanavalin A and phytohemagglutinin are summed up in the time. Under given experimental conditions each of the mitogens used did not induce mitogenesis when the interaction with lymphocytes continued for 14 and/or 20 hours. The results obtained are discussed from the point of view of the cell-to-cell interaction between the lymphocytes. It is assumed that the ability of the stimuli to sum up in the time underlies the nonspecific mechanism of defense from tolerance.