Temporal integration of sound motion: Motion-onset response and perception.

The purpose of our study was to estimate the time interval required for integrating the acoustical changes related to sound motion using both psychophysical and EEG measures. Healthy listeners performed direction identification tasks under dichotic conditions in the delayed-motion paradigm. Minimal audible movement angle (MAMA) has been measured over the range of velocities from 60 to 360 deg/s. We also measured minimal duration of motion, at which the listeners could identify its direction. EEG was recorded in the same group of subjects during passive listening. Motion onset responses (MOR) were analyzed. MAMA increased linearly with motion velocity. Minimum audible angle (MAA) calculated from this linear function was about 2 deg. For higher velocities of the delayed motion, we found 2- to 3-fold better spatial resolution than the one previously reported for motion starting at the sound onset. The time required for optimal discrimination of motion direction was about 34 ms. The main finding of our study was that both direction identification time obtained in the behavioral task and cN1 latency behaved like hyperbolic functions of the sound's velocity. Direction identification time decreased asymptotically to 8 ms, which was considered minimal integration time for the instantaneous shift detection. Peak latency of cN1 also decreased with increasing velocity and asymptotically approached 137 ms. This limit corresponded to the latency of response to the instantaneous sound shift and was 37 ms later than the latency of the sound-onset response. The direction discrimination time (34 ms) was of the same magnitude as the additional time required for motion processing to be reflected in the MOR potential. Thus, MOR latency can be viewed as a neurophysiological index of temporal integration. Based on the findings obtained, we may assume that no measurable MOR would be evoked by slowly moving stimuli as they would reach their MAMAs in a time longer than the optimal integration time.

Brain oscillations evoked by sound motion.

The present study investigates the event-related oscillations underlying the motion-onset response (MOR) evoked by sounds moving at different velocities. EEG was recorded for stationary sounds and for three patterns of sound motion produced by changes in interaural time differences. We explored the effect of motion velocity on the MOR potential, and also on the event-related spectral perturbation (ERSP) and inter-trial phase coherence (ITC) calculated from the time-frequency decomposition of EEG signals. The phase coherence of slow oscillations increased with an increase in motion velocity similarly to the magnitude of cN1 and cP2 components of the MOR response. The delta-to-alpha inter-trial spectral power remained at the same level up to, but not including, the highest velocity, suggesting that gradual spatial changes within the sound did not induce non-coherent activity. Conversely, the abrupt sound displacement induced theta-alpha oscillations which had low phase consistency. The findings suggest that the MOR potential could be mainly generated by the phase resetting of slow oscillations, and the degree of phase coherence may be considered as a neurophysiological indicator of sound motion processing.

Attention and speech-processing related functional brain networks activated in a multi-speaker environment.

Human listeners can focus on one speech stream out of several concurrent ones. The present study aimed to assess the whole-brain functional networks underlying a) the process of focusing attention on a single speech stream vs. dividing attention between two streams and 2) speech processing on different time-scales and depth. Two spoken narratives were presented simultaneously while listeners were instructed to a) track and memorize the contents of a speech stream and b) detect the presence of numerals or syntactic violations in the same ("focused attended condition") or in the parallel stream ("divided attended condition"). Speech content tracking was found to be associated with stronger connectivity in lower frequency bands (delta band- 0,5-4 Hz), whereas the detection tasks were linked with networks operating in the faster alpha (8-10 Hz) and beta (13-30 Hz) bands. These results suggest that the oscillation frequencies of the dominant brain networks during speech processing may be related to the duration of the time window within which information is integrated. We also found that focusing attention on a single speaker compared to dividing attention between two concurrent speakers was predominantly associated with connections involving the frontal cortices in the delta (0.5-4 Hz), alpha (8-10 Hz), and beta bands (13-30 Hz), whereas dividing attention between two parallel speech streams was linked with stronger connectivity involving the parietal cortices in the delta and beta frequency bands. Overall, connections strengthened by focused attention may reflect control over information selection, whereas connections strengthened by divided attention may reflect the need for maintaining two streams in parallel and the related control processes necessary for performing the tasks.

Mismatch negativity and psychophysical detection of rising and falling intensity sounds.

Human subjects demonstrate a perceptual priority for rising level sounds compared with falling level sounds. The aim of the present study was to investigate whether or not the perceptual preference for rising intensity can be found in the preattentive processing indexed by mismatch negativity (MMN). Reversed oddball stimulation was used to produce MMNs and to test the behavioral discrimination of rising, falling and constant level sounds. Three types of stimuli served as standards or deviants in different blocks: constant level sounds and two kinds of rising/falling sounds with gradual or stepwise change of intensity. The MMN amplitudes were calculated by subtracting ERPs to identical stimuli presented as standard in one block and deviant in another block. Both rising and falling level deviants elicited MMNs which peaked after 250 ms and did not overlap with N1 waves. MMN was elicited by level changes even when the deviants were not discriminated behaviorally. Most importantly, we found dissociation between earlier and later stages of auditory processing: the MMN responses to the level changes were mostly affected by the direction of deviance (increment or decrement) in the sequence, whereas behavioral performance depended on the direction of the level change within the stimuli (rising or falling).

Genetic characterization of an H2N2 influenza virus isolated from a muskrat in Western Siberia.

Thirty-two muskrats (Ondatra zibethicus) were captured for surveillance of avian influenza virus in wild waterfowl and mammals near Lake Chany, Western Siberia, Russia. A/muskrat/Russia/63/2014 (H2N2) was isolated from an apparently healthy muskrat using chicken embryos. Based on phylogenetic analysis, the hemagglutinin and neuraminidase genes of this isolate were classified into the Eurasian avian-like influenza virus clade and closely related to low pathogenic avian influenza viruses (LPAIVs) isolated from wild water birds in Italy and Sweden, respectively. Other internal genes were also closely related to LPAIVs isolated from Eurasian wild water birds. Results suggest that interspecies transmission of LPAIVs from wild water birds to semiaquatic mammals occurs, facilitating the spread and evolution of LPAIVs in wetland areas of Western Siberia.

Cellular internalization and morphological analysis after intravenous injection of a highly hydrophilic octahedral rhenium cluster complex - a new promising X-ray contrast agent.

The octahedral cluster compound Na2 H8 [{Re6 Se8 }(P(C2 H4 CONH2 )(C2 H4 COO)2 )6 ] has been shown to be highly radio dense, thus becoming a promising X-ray contrast agent. It was also shown that this compound had low cytotoxic effect in vitro, low acute toxicity in vivo and was eliminated rapidly from the body through the urinary tract. The present contribution describes a more detailed cellular internalization assay and morphological analysis after intravenous injection of this hexarhenium cluster compound at different doses. The median lethal dose (LD50 ) of intravenously administrated compound was calculated (4.67 ± 0.69 g/kg). Results of the study clearly indicated that the cluster complex Hn [{Re6 Se8 }(P(C2 H4 CONH2 )(C2 H4 COO)2 )6 ]n-10 was not internalized into cells in vitro and induced only moderate morphological alterations of kidneys at high doses without any changes in morphology of liver, spleen, duodenum, or heart of mice. Copyright © 2016 John Wiley & Sons, Ltd.

Experimental infection and pathology of clade 2.2 H5N1 virus in gulls.

During 2006, H5N1 HPAI caused an epizootic in wild birds, resulting in a die-off of Laridae in the Novosibirsk region at Chany Lake. In the present study, we infected common gulls (Larus canus) with a high dose of the H5N1 HPAI virus isolated from a common gull to determine if severe disease could be induced over the 28 day experimental period. Moderate clinical signs including diarrhea, conjunctivitis, respiratory distress and neurological signs were observed in virus-inoculated birds, and 50% died. The most common microscopic lesions observed were necrosis of the pancreas, mild encephalitis, mild myocarditis, liver parenchymal hemorrhages, lymphocytic hepatitis, parabronchi lumen hemorrhages and interstitial pneumonia. High viral titers were shed from the oropharyngeal route and virus was still detected in one bird at 25 days after infection. In the cloaca, the virus was detected sporadically in lower titers. The virus was transmitted to direct contact gulls. Thus, infected gulls can pose a significant risk of H5N1 HPAIV transmission to other wild migratory waterfowl and pose a risk to more susceptible poultry species. These findings have important implications regarding the mode of transmission and potential risks of H5N1 HPAI spread by gulls.

Do audio-visual motion cues promote segregation of auditory streams?

An audio-visual experiment using moving sound sources was designed to investigate whether the analysis of auditory scenes is modulated by synchronous presentation of visual information. Listeners were presented with an alternating sequence of two pure tones delivered by two separate sound sources. In different conditions, the two sound sources were either stationary or moving on random trajectories around the listener. Both the sounds and the movement trajectories were derived from recordings in which two humans were moving with loudspeakers attached to their heads. Visualized movement trajectories modeled by a computer animation were presented together with the sounds. In the main experiment, behavioral reports on sound organization were collected from young healthy volunteers. The proportion and stability of the different sound organizations were compared between the conditions in which the visualized trajectories matched the movement of the sound sources and when the two were independent of each other. The results corroborate earlier findings that separation of sound sources in space promotes segregation. However, no additional effect of auditory movement per se on the perceptual organization of sounds was obtained. Surprisingly, the presentation of movement-congruent visual cues did not strengthen the effects of spatial separation on segregating auditory streams. Our findings are consistent with the view that bistability in the auditory modality can occur independently from other modalities.

Mismatch negativity evoked by stationary and moving auditory images of different azimuthal positions.

The present study has been designed to evaluate the pre-attentive detection of the location changes for stationary and moving sound sources. Auditory event-related potentials to the click trains simulating stationary and moving fused auditory images were recorded from healthy subjects using an oddball paradigm. The spatial characteristics of stimuli were created by introducing constant or variable interaural time delay (ITD) into the click trains. Repetitive standard auditory images (0 or 800 micros ITD, p=0.9) were interspersed by infrequent deviants (p = 0.1) of three types: stationary and moving to or from standards. The deviants moving to standards elicited similar mismatch negativities (MMNs) as compared to the stationary ones. The deviants moving from standards evoked the lowest and latest MMNs depending on standard location. Results suggest that pre-attentive ITD discrimination is essentially dependent on the pattern of ITD changes at the moment of the deviant onset.