[Possible relationship between the cytologic characteristics of mouse leukemia P-388 cells and their sensitivity to rubomycin]. / Vozmozhnaia sviaz' mezhdu tsitologicheskimi kharakteristikami opukholevykh kletok leikoza P-388 myshei i ikh chuvstvitel'nost'iu k rubomitsinu.

In search for markers of tumor cell resistance to cytostatic drugs, cytologic characteristics were compared of resistant and sensitive to rubomycin variants of mouse P-388 leukemia. Morphocytochemical, morphometrical and cytogenetic investigations were performed as well as measurements of intracellular pH. As shown by the fluorescent test, pH levels in rubomycin-sensitive and resistant subpopulations differed. This fact evidences an important role of intracellular pH in inducing the resistance and revealed some mechanisms of rubomycin-resistant tumor cell selection.

[Seasonal changes in lysosomal pH of peripheral blood cells of healthy subjects]. / Sezonnye izmeneniia vnutrilizosomal'nogo pH v kletkakh perifericheskoi krovi zdorovykh liudei.

We studied pH values in lysosomes of peripheral blood white cells of 49 blood donors using the two-wavelengths (470 and 450 nm) microspectrophotometry of neutral red after supravital in vitro staining. In polymorphonuclear leucocytes lysosomal pH reaches the highest values in August-September (6.98 +/- 0.04) and the lowest--in February-March (6.23 +/- 0.09), p less than 0.05.

[Role of intracellular pH in the development of resistance of leukemic cells to antineoplastic drugs]. / Rol' vnutrikletochnogo pH v vozniknovenii ustoichivosti leikoznykh kletok k protivoopukholevym preparatam.

Intracellular pH (pHi) of mouse lymphoid leukemia P388 cells was measured at the wavelength of 518 and 570 nm of fluorescein: pHi of rubomycin-sensitive P388 cells was higher than pHi of the cells resistant to this antitumour antibiotic (6.98 +/- 0.04 and 6.63 +/- 0.03, respectively, P less than 0.001). The role of pHi is established during induction of the resistance of tumour cells to the antitumour drugs.

[Changes in the pH of leukocyte granules during the storage of preserved donor blood]. / Izmenenie pH granul leikotsitov v protsesse khraneniia konservirovannoi donorskoi krovi.

Data are presented on pH values in lymphocyte and neutrophil granules, as well as parameters of fluctuating pH changes in individual granules at early terms of storage (up to 72 h), evaluated by cytophotometry with the use of the vital stain neutral red in microspectrophotometer (Univar Reichert, Austria). An average value of pH in donor neutrophil granules was 6.57-0.12 (range 6.21-7.02). At day 3 of storage it decreased at average by 0.18 pH units. Rhythmical changes of pH in individual granules with pH 0.2-0.44 were found on all days of storage. The period of the rhythm lengthened from 3 min on day 1 to 4 min on day 3. This shift as well as acidification of granules during storage reflects their functional deterioration. The storage did not affect pH values in lymphocyte lysosomes which at average were 6.4, 6.5 and 6.35 on days 1, 2 and 3, respectively.