A comprehensive study for the potential removal of 152+154Eu radionuclides using a promising modified strontium-based MOF.

A facile modification of a strontium-based MOF using oxalic acid was carried out to prepare MTSr-OX MOF, which was used as a potential substance for eliminating 152+154Eu radioisotopes. Various analytical techniques were used to characterize MTSr-OX-MOF. The prepared MOF had a rod-like structure with a BET surface area of 101.55 m2 g-1. Batch sorption experiments were used to investigate the sorption performance of MTSr-OX-MOF towards 152+154Eu radionuclides where different parameters like pH, contact time, initial 152+154Eu concentration, ionic strength, and temperature were scrutinized to determine the optimum conditions for 152+154Eu removal. MTSr-OX-MOF showed superior effectiveness in the elimination of 152+154Eu with a maximum sorption capacity of 234.72 mg g-1 at pH 3.5. Kinetics fitted with the pseudo-second-order model and the Langmuir model correctly described the sorption mechanism. The thermodynamic variables were carefully examined, demonstrating that the 152+154Eu sorption was endothermic as well as spontaneous. The MTSr-OX-MOF has been found to be a significantly more effective sorbent towards 152+154Eu than that of many other adsorbents. When applied to real active waste, MTSr-OX-MOF demonstrated excellent removal performance for a wide range of radionuclides. As a result, the MTSr-OX-MOF can be recognized as an attractive solution for the 152+154Eu purification from active waste.

Correction: Novel sensor for the determination of CA 15-3 in serum of breast cancer patients based on Fe-gallic acid complex doped in modified cellulose polymer thin films.

[This corrects the article DOI: 10.1039/D3RA02495D.].

Recent progress in high-performance environmental impacts of the removal of radionuclides from wastewater based on metal-organic frameworks: a review.

The nuclear industry is rapidly developing and the effective management of nuclear waste and monitoring the nuclear fuel cycle are crucial. The presence of various radionuclides such as uranium (U), europium (Eu), technetium (Tc), iodine (I), thorium (Th), cesium (Cs), and strontium (Sr) in the environment is a major concern, and the development of materials with high adsorption capacity and selectivity is essential for their effective removal. Metal-organic frameworks (MOFs) have recently emerged as promising materials for removing radioactive elements from water resources due to their unique properties such as tunable pore size, high surface area, and chemical structure. This review provides an extensive analysis of the potential of MOFs as adsorbents for purifying various radionuclides rather than using different techniques such as precipitation, filtration, ion exchange, electrolysis, solvent extraction, and flotation. This review discusses various MOF fabrication methods, focusing on minimizing environmental impacts when using organic solvents and solvent-free methods, and covers the mechanism of MOF adsorption towards radionuclides, including macroscopic and microscopic views. It also examines the effectiveness of MOFs in removing radionuclides from wastewater, their behavior on exposure to high radiation, and their renewability and reusability. We conclude by emphasizing the need for further research to optimize the performance of MOFs and expand their use in real-world applications. Overall, this review provides valuable insights into the potential of MOFs as efficient and durable materials for removing radioactive elements from water resources, addressing a critical issue in the nuclear industry.

A novel test device and quantitative colorimetric method for the detection of human chorionic gonadotropin (hCG) based on Au@Zn-salen MOF for POCT applications.

The human chorionic gonadotropin (hCG) hormone is a biomarker that can predict tumors and early pregnancy; however, it is challenging to develop sensitive qualitative-quantitative procedures that are also effective, inventive, and unique. In this study, we used a novel easy in situ reaction of an organic nano-linker with Zn(NO3)2·6H2O and HAuCl4·3H2O to produce a gold-zinc-salen metal-organic framework composite known as Au-Zn-Sln-MOF. A wide variety of micro-analytical instruments and spectroscopic techniques were used in order to characterize the newly synthesized Au-Zn-Sln-MOF composite. Disclosure is provided for a novel swab test instrument and a straightforward colorimetric approach for detecting hCG hormone based on an Au-Zn-Sln-MOF composite. Both of these methods are easy. In order to validate a natural enzyme-free immunoassay, an Au-Zn-Sln-MOF composite was utilized in the role of an enzyme; a woman can use this gadget to determine whether or not she is pregnant in the early stages of the pregnancy or whether or not her hCG levels are excessively high, which is a symptom that she may have a tumor. This cotton swab test device is compatible with testing of various biological fluids, such as serum, plasma, or urine, and it can be easily transferred to the market to commercialize it as a costless kit, which will be 20-30% cheaper than what is available on the market. Additionally, it can be used easily at home and for near-patient testing (applications of point-of-care testing (POCT)).

Prostate-Specific Antigen Monitoring Using Nano Zinc(II) Metal-Organic Framework-Based Optical Biosensor.

BACKGROUND: The prostate-specific antigen (PSA) is an important cancer biomarker that is commonly utilized in the diagnosis of prostate cancer. The development of a PSA determination technique that is rapid, simple, and inexpensive, in addition to highly accurate, sensitive, and selective, remains a formidable obstacle. METHODS: In this study, we developed a practical biosensor based on Zn(II) metal-organic framework nanoparticles (Zn-MOFs-NPs). Many spectroscopic and microanalytical tools are used to determine the structure, morphology, and physicochemical properties of the prepared MOF. RESULTS: According to the results, Zn-MOFs-NPs are sensitive to PSA, selective to an extremely greater extent, and stable in terms of chemical composition. Furthermore, the Zn-MOFs-NPs did not exhibit any interferences from other common analytes that might cause interference. The detection limit for PSA was calculated and was 0.145 fg/mL throughout a wide linear concentration range (0.1 fg/mL-20 pg/mL). CONCLUSIONS: Zn-MOFs-NPs were successfully used as a growing biosensor for the monitoring and measurement of PSA in biological real samples.

Smart prototype for an electronic color sensor device for visual simultaneous detection of macrofuran based on a coated paper strip.

Nowadays, in the clinical, pharmaceutical, and environmental sectors, the development of facile and sensitive analytical methods and/or innovative devices for the follow-up and detection of antibiotics and pharmaceutical formulations, in general, are urgently needed and still challenging. This work declared three vital applications for broad-spectrum nitrofurantoin (macrofuran) antibiotic detection and quantification: A colorimetric method, a coated paper strip-based nano-lanthanum complex prototype and fabrication of smart electronic color sensor device-based coated paper strips. The colorimetric method showed a significant response upon increasing the concentration of the nitrofurantoin in a range between (1.0-100.0 ng/mL) via a visual color change from orange-yellow to red colors degree with detection and quantification limits of 0.175 and 0.53 ng/mL, respectively, whereas the nano-lanthanum complex coated paper strip prototype showed qualitative on-site sensing for nitrofurantoin via naked eye color changes which can be detected anywhere. Moreover, a smart prototype for detecting macrofuran in the means of paper color change in the RGB color component extraction algorithm and the grayscale projection value processing algorithm was fabricated. The change in RGB color on the coated paper strip was detected using an electronic color sensor device. The developed colorimetric method, coated paper strip, and the electronic color sensor device prototype exhibited fast, simple, costless, and selective towards macrofuran over the competing analyzed. As well as, showed good applicability in the different real samples spiked with different concentrations of macrofuran.

A novel strontium-based MOF: synthesis, characterization, and promising application in removal of 152+154Eu from active waste.

Removal of hazardous radioactive materials such as 152+154Eu from active waste using the batch approach has attracted attention nowadays. In this work, a novel melamine-terephthalic strontium metal-organic framework (MTSr-MOF) was prepared via a hydrothermal method. The MTSr-MOF was characterized by various analytical techniques such as FT-IR, 1H/13C-NMR, mass spectroscopy, XPS, XRD, TGA, BET, FE-SEM/EDX, TEM, and UV. The obtained data revealed that MTSr-MOF exhibited brick-like building blocks that were bridged together by the linkers, and each block had a thickness of ∼120 nm. The BET surface area was 74.04 m2 g-1. MTSr-MOF was used for the removal of 152+154Eu radionuclides from active waste. Further functionalization using various modifiers, including oxalic acid, EDTA, sulfuric acid, and sodium hydroxide was carried out to improve the sorption efficiency of MTSr-MOF towards 152+154Eu radionuclides. Among them, MTSr-MOF modified with oxalic acid (MTSr-OX-MOF) demonstrated a superior removal efficiency toward 152+154Eu radionuclides when compared to MTSr-MOF or other published reports, with a removal efficiency of more than 96%. The higher sorption efficiency of the MTSr-OX-MOF indicates that it could be a promising candidate for the removal of 152+154Eu radionuclides from radioactive waste.

Nanomaterials and metal-organic frameworks for biosensing applications of mutations of the emerging viruses.

The world today lives in a state of terrible fear due to the mutation of the emerging COVID-19. With the continuation of this pandemic, there is an urgent need for fast, accurate testing devices to detect the emerging SARS-CoV-2 pandemic in terms of biosensors and point-of-care testing. Besides, the urgent development in personal defense tools, anti-viral surfaces and wearables, and smartphones open the door for simplifying the self-diagnosis process everywhere. This review introduces a quick COVID-19 overview: definition, transmission, pathophysiology, the identification and diagnosis, mutation and transformation, and the global situation. It also focuses on an overview of the rapidly advanced technologies based on nanomaterials and MOFs for biosensing, diagnosing, and viral control of the SARS-CoV-2 pandemic. Finally, highlight the latest technologies, applications, existing achievements, and preventive diagnostic strategies to control this epidemic and combat the emerging coronavirus. This humble effort aims to provide a helpful survey that can be used to develop a creative solution and to lay down the future vision of diagnosis against COVID-19.

Long-term histopathological changes of the nasal mucosa after total laryngectomy: a prospective cohort study.

BACKGROUND: Changes in the nasal function following total laryngectomy resulted in histopathological alterations of the nasal mucosa. We aimed to evaluate the long-term histopathological changes and the mucociliary clearance (MCC) of the nasal mucosa after total laryngectomy. METHODS: We performed a histological examination of inferior turbinate biopsy, and saccharine test to assess the MCC time for patients who were candidates for total laryngectomy before the procedure, 6-12 months after surgery, and at least two years postoperatively. RESULTS: Seventy-five patients scheduled for total laryngectomy were initially enrolled in our study. We excluded patients who received postoperative radiotherapy or were lost during the follow-up period. Eventually, 63 and 54 patients were available for assessment 6-12 months after surgery and at least two years postoperatively, respectively. Except for ciliary and goblet cell destruction, which were significantly reduced 6-12 months postoperatively, there were no statistically significant differences in the histopathological findings of the nasal mucosa before surgery and 6-12 months postoperatively. After two years, the histopathological alterations of the nasal mucosa were statistically more evident than those before surgery and 6-12 months postoperatively; the most common histopathological findings were mononuclear cell infiltration and stromal fibrosis. The mean MCC time preoperatively was 12.56 minutes that statistically significantly decreased to 11.81 minutes 6-12 months after surgery; then, it significantly increased to 20.98 minutes at least two years postoperatively. CONCLUSIONS: After total laryngectomy, the nasal mucosa showed histopathological alterations and early enhancement of the MCC, which was later impaired due to nasal mucosal atrophy and the saprophytic infection.

New approach in SARS-CoV-2 surveillance using biosensor technology: a review.

Biosensors are analytical tools that transform the bio-signal into an observable response. Biosensors are effective for early detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection because they target viral antigens to assess clinical development and provide information on the severity and critical trends of infection. The biosensors are capable of being on-site, fast, and extremely sensitive to the target viral antigen, opening the door for early detection of SARS-CoV-2. They can screen individuals in hospitals, airports, and other crowded locations. Microfluidics and nanotechnology are promising cornerstones for the development of biosensor-based techniques. Recently, due to high selectivity, simplicity, low cost, and reliability, the production of biosensor instruments have attracted considerable interest. This review article precisely provides the extensive scientific advancement and intensive look of basic principles and implementation of biosensors in SARS-CoV-2 surveillance, especially for human health. In this review, the importance of biosensors including Optical, Electrochemical, Piezoelectric, Microfluidic, Paper-based biosensors, Immunosensors, and Nano-Biosensors in the detection of SARS-CoV-2 has been underscored. Smartphone biosensors and calorimetric strips that target antibodies or antigens should be developed immediately to combat the rapidly spreading SARS-CoV-2. Wearable biosensors can constantly monitor patients, which is a highly desired feature of biosensors. Finally, we summarized the literature, outlined new approaches and future directions in diagnosing SARS-CoV-2 by biosensor-based techniques.

Correction: Ultrafast conversion of carcinogenic 4-nitrophenol into 4-aminophenol in the dark catalyzed by surface interaction on BiPO4/g-C3N4 nanostructures in the presence of NaBH4.

[This corrects the article DOI: 10.1039/D1RA02852A.].

A novel nano-lanthanum complex: synthesis, characterization and application as a macrofuran chemosensor in pharmaceutical, biological and environmental samples.

Macrofuran is widely used as an antibiotic for the treatment of urinary tract infections. Nevertheless, it is prohibited due to toxicity and environmental concerns. The development of a fast, simple, and cost-effective approach for the determination of macrofuran antibiotic (MFA) is still a challenge. Herein, we report a chemosensor based on a nano-lanthanum complex derived from phenylenediamine. The physicochemical properties and structure of the prepared complex were confirmed using different spectroscopic tools such as X-ray diffraction (XRD), scanning electron microscopy equipped with EDX, elemental analysis, Fourier transform-infrared (FT-IR) spectroscopy, UV-vis spectroscopy, mass spectroscopy and photoluminescence (PL). The nano-lanthanum complex was found to be chemically stable, highly sensitive and selective to MFA, without interference from other common antibiotics. The limit of detection for MFA was 0.025 ng mL-1, over a linear concentration range of 0.02-30.0 ng mL-1, with a correlation coefficient of 0.994. The nano-lanthanum complex can be used successfully as a promising chemosensor for MFA determination in pharmaceutical formulation and different biological samples (whole blood-serum-plasma). In addition, this approach will protect human beings from the environmental hazards of antibiotics through the detection of the low limit of MFA. Meanwhile, the mechanism of interaction between the nano-lanthanum complex and MFA was studied and investigated.

Ultrafast conversion of carcinogenic 4-nitrophenol into 4-aminophenol in the dark catalyzed by surface interaction on BiPO4/g-C3N4 nanostructures in the presence of NaBH4.

The heterogeneous catalytic conversion of pollutants into useful industrial compounds is a two-goals at once process, which is highly recommended from the environmental, economic, and industrial points of view. In this regard, design materials with high conversion ability for a specific application is required to achieve such a goal. Herein, the synthesis conditions for the fabrication of BiPO4 nanorod bundles supported on g-C3N4 nanosheets as heterojunction composites was achieved using a facile ex situ chemical deposition for the reductive conversion of carcinogenic 4-nitrophenol (4-NP) into 4-aminophenol (4-AP). To better understand the mechanistic reduction pathways, BiPO4/g-C3N4 composites with varying ratios where obtained. The morphology and structure of BiPO4/g-C3N4 composites were checked using several methods: XRD, FE-SEM, HRTEM, XPS, and FT-IR, and it was found that hexagonal phase BiPO4 nanorod bundles were randomly distributed on the g-C3N4 nanosheets. Overall, the reduction ability of BiPO4/g-C3N4 composites was far better than bare BiPO4 and g-C3N4. A total reductive conversion of 4-NP at a concentration of 10 mg L-1 into 4-AP was found with 50% BiPO4/g-C3N4 composite within only one minute of reaction. Moreover, the presence of reducing agent (NaBH4) enhanced the kinetic rate constant up to 2.914 min-1 using 50% BiPO4/g-C3N4, which was much faster than bare BiPO4 (0.052 min-1) or g-C3N4 (0.004 min-1). The effects of some operating parameters including the initial concentration of 4-NP and catalyst dosage were also evaluated during the experiments. BiPO4/g-C3N4 showed great stability and recyclability, wherein, the catalytic reduction efficiency remains the same after five runs. A plausible 4-NP reduction mechanism was discussed. The high catalytic activity with the good stability of BiPO4/g-C3N4 make it a potential candidate for the reduction of nitroaromatic compounds in real wastewaters.

A novel nano copper complex: potentiometry, DFT and application as a cancer prostatic biomarker for the ultrasensitive detection of human PSA.

Worldwide, prostate cancer is considered to be one of the three most commonly occurring cancers amongst the male population. Clinically, early detection of diverse forms of cancer before they spread and become incurable plays an important role in treatment strategy. Therefore, the development of fast, accurate, sensitive, and low-cost analytical methodologies and techniques for the detection of cancer biomarkers is an attractive research area for scientists globally. Herein, a Schiff base ligand (A1) was prepared via the refluxing of 3-aminobenzoic acid with 1,2-phenylenediamine. After that, a nano Cu complex (N1) was synthesized by reacting A1 with copper chloride. The produced A1 and N1 were characterized using several techniques to determine their physicochemical properties. A density functional theory study was carried out to rationalize the experimental work and support the obtained results. Moreover, the nano Cu complex (N1) was used for the fabrication of a potentiometric membrane biosensor for the early detection of the prostate-specific antigen (PSA). The results reveal that the electrode displays a stable Nernstian response of 29.26 ± 0.87 mV per decade for PSA in a linear dynamic range of 5.0 pg mL-1-10.0 ng mL-1, in a pH range of 6.5-9.2, with a short response time of 25 ± 5 s. The lifetime was between 5-7 weeks under different storage conditions. The detection (LOD) and quantification (LOQ) limits were 0.098 and 0.297 pg mL-1, respectively. The presence of different interfering species on the potentiometric biosensor response against PSA was investigated. The sensing mechanism of N1 toward PSA and the applicability of the developed electrode for the screening and quantification of PSA in real serum samples were also studied.

Dual Naked-Eye and Optical Chemosensor for Morphine Detection in Biological Real Samples Based on Cr(III) Metal-Organic Framework Nanoparticles.

The analytical detection and quantification of abuse drugs such as morphine (MOR) in biological samples are vital missions and remains to attract challenges for forensic toxicology, law enforcement, world antidoping organization, and social health fields. MOR, a benchmark analgesic drug known as "pain killer", is one of the powerful opioid medications for relieving pain, and overdose of MOR is toxic. In this article, novel promising chromium metal-organic framework nanoparticles [Cr(III)-MOF-NPs] were produced via facile synthesis and characterized using high-resolution transmission electron microscopy, field-emission scanning electron microscopy/energy-dispersive X-ray spectroscopy, mass spectrometry, X-ray photoelectron spectroscopy, elemental analysis, UV-vis, Fourier transform infrared, and thermogravimetry/differential scanning calorimetry, as well as photoluminescence (PL) investigation and magnetic properties. The PL study results revealed that the Cr(III)-MOF-NPs exhibited an emission band at 593 nm. The Cr(III)-MOF-NPs could be used in fast, selective, and sensitive MOR detection and quantification. Under the optimum experimental conditions, with the addition of MOR, a blueshift from 593 to 566 nm occurred with a remarkable PL intensity enhancement, and the color changed from brown to yellow (visually/naked-eye detection). The Cr(III)-MOF-NPs optical chemosensor exhibited a stable response for MOR in a concentration range between 0.1 and 350 nM. The detection and quantification limits were 0.167 and 0.443 nM, respectively, with a correlation coefficient (r 2) of 0.96. The developed PL chemosensor showed high selectivity for MOR over other competing interfering matrices. Moreover, the ultrasensitive chemosensor was extensively used for the determination of MOR spiked in different real samples (serum and urine samples) with acceptable recoveries and satisfactory results.

A novel HCV electrochemical biosensor based on a polyaniline@Ni-MOF nanocomposite.

Hepatitis-C virus ribonucleic acid (HCV-RNA) recognition and quantification based on real-time polymerase chain reaction (RT-PCR) is key to infection control, management, and response to treatment due to its specificity, sensitivity, and quantification capabilities. However, the high cost, time requirements, and need for sophisticated laboratory infrastructure have limited the use of this method in rapid screening, blood banks, and point-of-care testing (POCT). In this work, a novel label-free electrochemical biosensor constructed using a polyaniline@nickel metal-organic framework (Ni-MOF) nanocomposite was developed for direct detection of unamplified HCV nucleic acid. A robust biosensor was fabricated using smooth layer-by-layer deposition of the polyaniline@Ni-MOF nanocomposite, deoxyribonucleic acid (DNA) probe, and bovine serum albumin (BSA) onto a glassy carbon electrode (GCE) and was subsequently monitored real-time via cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The sensitivity and specificity of the newly developed biosensor were specifically examined using the EIS approach. The results revealed that the novel biosensor is highly efficient in quantitative sensing of the HCV target in the presence of nonspecific nucleic acids over the range of 1 fM-100 nM with a detection limit of 0.75 fM (at a S/N ratio of 3). To the best of the authors' knowledge, the proposed biosensor is superior to other MOF platforms. These research findings are expected to have a positive influence on the quantitative detection of HCV RNA and other nucleic acids by offering exceptional accuracy and cost effectiveness, especially in low resource countries. Moreover, this biosensor could be simply adopted for full automation and used in point-of-care testing.

Correction to: Novel advanced nanomaterial based on ferrous metal-organic framework and its application as chemosensors for mercury in environmental and biological samples.

The authors would like to call the reader's attention to the fact that, unfortunately, there was an oversight regarding the Acknowledgment in this manuscript; please find the correct information below.

Novel advanced nanomaterial based on ferrous metal-organic framework and its application as chemosensors for mercury in environmental and biological samples.

In this work, promising novel ferrous metal-organic framework nanoparticles (Fe(II)-MOF-NPs) were prepared via a simple method. The produced materials were fully characterized using FE-SEM, EDX, HR-TEM, elemental analysis, mass spectrometry, FT-IR, UV-Vis, TG/DSC, XRD, XPS, and analysis of magnetic properties. Colorimetric and photoluminescence (PL) investigations of Fe(II)-MOF-NPs were also carried out. The specific coordination binding between the Hg(II) and amino groups of the MOF led to an enhancement of the PL and the absorbance intensities. Therefore, Hg(II) concentrations could be determined quantitatively. A fast, sensitive, and selective method of mercury ion detection based on colorimetric and PL chemosensors using Fe(II)-MOF-NPs was developed. At optimal conditions, the PL and colorimetric chemosensors exhibited stable responses for Hg(II) in a concentration range of 1.0 nM to 1.0 µM with detection limits (LOD) = 1.17 and 1.14 nM and quantification limits (LOQ) = 1.59 and 1.48 nM, respectively. The developed PL and colorimetric chemosensors exhibited high selectivity towards Hg(II) over the other competing metal ions. Moreover, both ultrasensitive chemosensors were further investigated for determination of Hg(II) in different water sources (tap, mineral, river, sea, and wastewater) as well as in biological samples (blood serum and urine samples), with satisfactory recoveries. Graphical abstract.

A novel cerium(iii)-isatin Schiff base complex: spectrofluorometric and DFT studies and application as a kidney biomarker for ultrasensitive detection of human creatinine.

In this paper, a new isatin-Schiff base L1 was prepared via a simple reaction of isatin with 2-amino-3-hydroxypyridin. Subsequently, cerium(iii)-Schiff base complex C1 was obtained through the reaction of the prepared Schiff base L1 with cerium chloride via a hydrothermal method. The prepared L1, as well as C1, were fully characterized using many spectroscopic techniques, such as mass spectra, elemental analysis, UV-vis, FT-IR, 1H-NMR, 13C-NMR, FE-SEM/EDX, and HR-TEM. A photoluminescence study (PL) was carried out for the prepared complex C1. The promising photoluminescence results revealed that C1 could be used for the detection of creatinine in different human biological samples as a selective optical biosensor. The results showed that C1 after excitation at 370 nm has a strong emission band at 560 nm. The calibration graph was obtained in a wide concentration range between 2.5 and 480 nM creatinine with limits of detection (LOD) and quantitation (LOQ) of 1.07 and 3.25 nM, respectively. In addition, the correlation coefficient (r 2) was found to be 0.9890. The PL spectra indicate that C1 has high selectivity toward creatinine without interference from other different analytes and can be successfully used as an optical sensor for creatinine detection. The mechanism of quenching between the Ce(iii) complex and creatinine was a dynamic type. The geometry of Schiff base L1 and its cerium(iii) complex C1 was proven by using density functional theory (DFT). The energy of the LUMO and HOMO, energy gap, dipole moment and structure-activity relationship were determined and confirmed.

Nucleic acids biosensors based on metal-organic framework (MOF): Paving the way to clinical laboratory diagnosis.

Development of ultra-sensitive, high specific and cost-effective nucleic acids (NAs) biosensors is critical for early diagnosis of cancer, genetic diseases and follows up response to treatment. Metal-organic frameworks (MOFs) as sensing materials underwent significant development in recent years due to their unique merits, such as structural diversity, tunable pore scale, large surface area, remarkable adsorption affinities, and good thermal stability. MOFs have shown potential contribution in nucleic acids biosensors research. Herein, a comprehensive overview on NAs biosensors state of the art based on MOFs has been discussed extensively, including different MOFs platforms sensing strategies (fluorescence, electrochemistry, electrochemiluminescence, and colorimetric techniques), their analytical performance and figures of merit in clinical diagnostics, with the future perspective in introducing MOFs in clinical laboratory diagnostics. Moreover, the different MOFs synthesis methods have been highlighted to serve as a guide for the researchers in selecting the appropriate platform that suits their research needs, and applications.