[Modulation of an antituberculous immune response in vitro, by using antigen-activated dendritic cells].

The protocol for preparation of mature antigen-activated dendritic cells (DC) from peripheral blood monocytes from patients with pulmonary tuberculosis was optimized. The obtained DCs were concurrently cultured with mononuclear cells (MNC) to activate antigen recognition, by and without adding IL-18 for the formation of directed differentiation of naive T cells to T helper cells type 1 (Th1). Joint cultivation of specific autologous DCs and MNCs from patients with pulmonary tuberculosis is an effective way of activation of the latter, which appeared as an increase in the proliferative potential, stimulation of IFN-gamma production and formation of cytotoxic cells expressing perforin in response to the specific Mycobacterium tuberculosis antigen ESAT-6. The use of recombinant IL-18 to enhance the induction of a Th1 response statistically significantly increases all the study parameters of MNC activation.

[Stress-hyperresponsive period in postnatal development of hypothalamic-hypophyseal-adrenal system in mice].

OBJECTIVE OF THE STUDY: to compare response to stress, activity of adenylatcyclase and enzymes of steroidogenesis in adrenal cells in 3-week and adult (15-week) mice. In 3-week mice, basal and stressor level of blood corticosterone was increased. Their response to stress was faster developing as compared with adult mice. In vitro, 3-week mice had enhanced adrenal sensitivity to ACTH, corticosterone release in response to ACTH, exogenous cAMP and progesterone; and the cAMP level in the cells stimulated with ACTH and forscoline. Thus, in 3-week mice, a stress-hyperresponsive period occurs due to increased release of corticosterone and adrenal sensitivity to ACTH. The reason of this involves enhanced activity of adenylatcyclase and intracellular enzymes of steroidogenesis.

[Allele Frequency Analysis of Four Single Nucleotide Polymorphisms Locating in Promoter and 5'-Untranslated Regions of ABCAI Gene in Young Men - Survivors From Myocardial Infarction]. / Analiz chastoty vyiavleniia chetyrekh strukturnykh polimorfizmov DNK v promotornoi i 5'-netransliruemykh oblastiakh gena ABCA1 u muzhchin, perenesshikh infarkt miokarda v molodom vozraste.

Analysis of allele distribution of four single nucleotide polymorphisms (C-17G, C69T, G-191C and 319insG) of promoter and 5'-untranslated regions of the ABCA1 gene was carried out in a sample of 171 men, who had survived myocardial infarction before 45 years, and in controls. Two-fold increase of T69 and C-191 allele frequencies were observed in Russian population in comparison to Dutch one. While comparing allele and genotype distributions of the polymorphisms in the samples under study no statistically significant differences were found, so as no influence of different alleles on lipid spectrum data was observed. Role of polymorphisms under study appears to be insignificant in formation of genetic susceptibility to myocardial infarction in young men.