[Morphology and structural peculiarities of Basidiomycetes pathogens].

The materials of studies of morphology and structural peculiarities of viruses, fungi and bacteria, which affect Basidiomycetes under biotechnology process and nature biocenosis conditions are given. The analysis of infection development in button mushroom (Agaricus bisporus) (J.Lge) Imbach and in oyster mushroom (Pleurotus ostreatus Kumm.), which served as model objects in the experiments of various levels of complexity has been carried out. Other kinds of edible and medicinal mushrooms, which were a source of biochemical fractions to form biologicals were investigated.

[Characteristics of green mottle mosaic virus isolates in cucumbers from different regions of Ukraine].

Isolates of Cucumber green mottle mosaic virus (CGMMV) obtained from three greenhouses of Ukraine were identified. The rabbit antisera have been obtained for these isolates. These antisera can be used for diagnostics of CGMMV in plant material by indirect ELISA. An analysis of cDNA sequences of capsid protein of these viruses confirmed that they might be grouped into the novel strain of CGMMV.

[The course of virus infection of Lycopersicon esculentum plants at high concentrations of heavy metals]. / Perebih virusnoï infektsiï u roslyn Lycopersicon esculentum pri vysoykh kontsentratsiakh vazhkykh metaliv.

Effect of certain compositions of heavy metals on the course of the system infection of TMV in plants of tomatoes Lycopersicon esculentum L. under field conditions was investigated. It is shown that the simultaneous influence of the virus infection and heavy metals in concentration of 10 MPC (maximum permissible concentration) resulted in the decrease of the content of total chlorophyll in the investigated plants by degradation of chlorophyll A; that was more considerable decrease as compared with separate influence of each of the factors. Treatment of plants by toxical concentrations of lead and zinc led to inconsiderable delay in development of the system TMV infection together with the increase of the virus content in plants 2 and more times, that evidences for the probable synergism in the action of the given heavy metals and virus infection. On the contrary, copper, though it decreased the content of total chlorophyll, manifested the protective properties and restricted essentially the virus accumulation in a plant as a whole.

Detection of plant viruses using a surface plasmon resonance via complexing with specific antibodies.

The use of instrumental systems based on the surface plasmon resonance (SPR) for rapid diagnosis of intact plant viruses (in particular, tobacco mosaic virus (TMV)) is considered. A new approach using detection of viral antigen and antibody (IgG) complexes formed during the preincubation step (instead of their consecutive application in classical approach) is discussed. A comparison between signal level registered from the mixture of virus and specific serum and that from the sample without virus (samples deposited onto the sensor surface treated with thiocyanate and protein A Staphylococcus aureus) allows unambiguous detection of viral particles in the material studied. The performance capabilities of the method are discussed and illustrated by quantitative detection of virus in the actual samples (cells homogenate) at high concentration.

[Analysis of functional and biochemical changes in feline cerebral hemispheres after subchronic haloperidol administration]. / Analiz funktsional'nykh i biokhimicheskikh izmenenii v polushariiakh mozga u koshek posle subkhronicheskogo vvedeniia galoperiodol.

Feline experiments have revealed that subchronic haloperidol causes asymmetric changes in the mesencephalic levels of dopamine and serotonin and impairs intrahemispheric balance between the dopaminergic and serotoninergic systems. At the same time the neuroleptic increases motor asymmetry, which appears in response to apomorphine, and changes the nature of interhemispheric relationships. The findings suggest that these changes may be one of the causes of extrapyramidal disorders associated with long-term use of the neuroleptic.

[The behavioral and neurochemical changes in rats caused by the subchronic administration of haloperidol]. / Povedencheskie i neirokhimicheskie izmeneniia u krys, vyzvannye subkhronicheskim vvedeniem galoperidola.

Rat experiments showed that subchronic haloperidol enhanced or perverted motor asymmetry appearing in response to apomorphine, resulted in imbalance between the dopaminergic and serotoninergic systems and increased norepinephrine levels. It was concluded that intra- and interhemispheric imbalance might be one of the causes of extrapyramidal disorders associated with the long-term administration of the neuroleptic.