ABSTRACT
The standardization of DNA fragment assembly methods for many tasks of synthetic biology is crucial. This is necessary for synthesizing a wider repertoire of sequences, as well as for further automation and miniaturization of such reactions. In this work, we proposed conditions for the assembly of DNA fragments from chemically synthesized oligonucleotides and we identified the errors occurring in the sequence under these conditions. Additionally, we proposed conditions for further combining synthetic fragments into larger DNA fragments. We showed that the optimized conditions are suitable for the assembly of a wide range of sequences.
ABSTRACT
Accurate distances between two trityl paramagnetic tags site-specifically attached to DNA duplexes were measured by pulsed electron-electron double resonance spectroscopy at 180 and 260 GHz microwave frequencies. Up to a threefold increase in the sensitivity of 260 GHz PELDOR measurements was achieved by using shaped broad-band microwave pulses.
Subject(s)
DNA/chemistry , Microwaves , Trityl Compounds/chemistry , Electron Spin Resonance Spectroscopy , Molecular Dynamics Simulation , Nucleic Acid Conformation , TemperatureABSTRACT
The problem of step-by-step control of radiotherapy of malignant tumors was solved on the basis of Ellis' concept, widely used in radiology. A new method for determining step parameters was proposed. An algorithm and an example of the calculation of parameters of a course of therapy in case of a heterogeneous tumor were given.
Subject(s)
Models, Biological , Neoplasms/radiotherapy , Radiotherapy Planning, Computer-Assisted , Radiotherapy, Computer-Assisted , Algorithms , Humans , Radiotherapy Dosage , SoftwareABSTRACT
A total of 128 patients with alimentary obesity were examined in the course of the treatment. There was a significant elevation in blood serum of total and free cholesterol, its esters, free fatty acids, beta-lipoproteins, and a decrease in the activity of serum lipase. The correlation of cholesterol esters to phospholipids was found to be disordered. Eighty patients demonstrated, after treatment with chloride sodium bromoiodine baths combined with hypocaloric diet and exercise therapy, a favourable clinical time course and normalization of the majority of lipid metabolism indicators, with an increase in serum lipase activity. Twenty-five patients, who received fresh baths under similar conditions, demonstrated less pronounced shifts in lipid metabolism, which were not always significant. The treatment complex including phepranon also gave rise to the normalization of the majority of lipid metabolism indicators. However, one-third of cases developed serious side effects.
