Rat Blood Leukocytes after Intravenous Injection of Magnetoliposomes on the Basis of Nanomagnetite.

The structure of blood neutrophils, eosinophils, monocytes, and lymphocytes and differential white blood count in adult rats were studied over 120 days after a single intravenous injection of magnetoliposomes based on nanomagnetite. Magnetoliposomes had no effect on the structure of neutrophils, eosinophils, monocytes, and lymphocytes. At the same time, injection of a suspension of magnetoliposomes based on magnetite nanoparticles led to a decrease in lymphocyte count and an increase in the count of monocytes and band and segmented neutrophils in the blood. These changes were transient and the parameters returned to normal by day 40-60 after injection.

Radiolabeled Complex of Antimicrobial Peptides UBI29-41 and UBI18-35 Labeled with 99mTc for Differential Diagnosis of Bone Infection of the Limbs.

We studied an original radiolabeled complex of antimicrobial peptides UBI29-41 and UBI18-35, ubiquicidin derivatives, for distinguishing between bacterial and aseptic inflammation. For radiolabeling of the peptides with technetium-99m, a bifunctional chelating agent succinimide-1-yl 6-(bis(pyridin-2-ylmethyl)amino)hexanoate was used. The obtained complexes 99mТс-DPAH-UBI29-41 and 99mТс-DPAH-UBI18-35 had radiolabeling yield >80% and radiochemical purity >96%. Accumulation of the complexes in the focus of bacterial inflammation in bone structures and the absence of this complex in the site of aseptic inflammation was confirmed in a rat model of traumatic osteomyelitis by single-photon emission computed tomography.

Rat Blood Leukocytes after Intravenous Injection of Chitosan-Modified Magnetic Nanospheres.

Nanosized magnetite particles (magnetic nanospheres) are a prospective basis for creation of new diagnostic and therapeutic agents. The structure of blood leukocytes and the leukocytic formula are studied in adult rats over a period of 120 days after a single intravenous injection of chitosan-modified nanosized magnetite particles. No effects of chitosan-modified magnetic nanospheres on the structure of rat blood leukocytes are detected. Injection of suspension of chitosan-modified magnetite nanospheres is associated with an increase in the levels of monocytes, segmented and stab neutrophils, and a decrease in lymphocyte counts in the blood of rats. The shifts in the leukogram parameters are transitory, the picture returned to normal by day 40 postinjection.

[Morphofunctional Changes of Spleen After Hemostasis by Nonequilibrium Plasma].

BACKGROUND: Heavy bleeding is developed in case of spleen injury. It often leads to death. At present the search and development of new methods for hemostasis of spleen bleeding continues. An innovative method is coagulation of nonequilibrium plasma. OBJECTIVE: Our aim was to study the effect of nonequilibrium plasma on the morphofunctional state of the spleen. METHODS: The non-randomized study by type of "case-control" was conducted. The experiment was carried out on 45 male rabbits weighing 3000-3200 g. 1st group (control; n = 5)--intact animals without surgery; 2nd group (experimental, n = 40)--animals after atypical resection of the spleen and hemostasis by nonequilibrium plasma. The volume of blood loss, FBC, histological data, splenogram analysis data were assessed at defined periods (60 min, 3rd, 5th, 7th, 14th, 30th, 90th, 180th days) after surgery. They were compared with data of the control group. RESULTS: The volume of blood loss during the resection of the spleen with hemostasis by nonequilibrium plasma was 16.6 [15.98; 17.22] ml. Increase of neutrophil to 38 [24; 39] (control group 17[13, 18], p = 0.009), monocytes to 15 [14, 18] (control group 8 [6, 11], p = 0.036) is revealed in the FBC in the early period after hemostasis with nonequilibrium plasma. It is reduced to the norm on 30 day. Histological examination revealed leukocyte infiltration, edema, microvascular stasis, dilatation of vessels in the early period. At long-term period structure of the organ is normalized. Splenogramm analysis revealed a statistically significant (p = 0.023) decrease in the relative number of small lymphocytes by 28% in animals after hemostasis with nonequilibrium plasma as compared to control (23.3 [179; 26.7] versus 30.8 [29.25; 34.3] respectively). CONCLUSION: treatment of bleeding surface by plasma flow for 1.5-2 min is required order to achieve effective hemostasis during surgery of spleen injuries in the experiment. After coagulation there is minimal damage of the spleen parenchyma, which manifests itself as activation reaction. At long-term period parenchyma ofthe spleen completely regenerates with theformation of cicatrical tissue that does not affect thefunctioning of organ.

Complex application of preparation containing ultralow doses of antibodies for the treatment of anemia caused by pubertal uterine bleedings.

Poetam and anaferon (pediatric formulation) administered in combination with iron preparation to patients with anemia induce pronounced positive shifts in metabolic and morphological status of mature erythrocytes: the number of sulfhydryl groups and lipoprotein complexes in cell membranes and dry weight of erythrocytes increased, while the number of forms at different stages of degeneration decreased.

[Evaluation of the effects of antituberculous drugs on the cytochemical status of lymphocytes in vitro].

The effects of basic drugs of chemotherapy for tuberclosis, such as isoniazid and rifampicin, on the activity of the most important mitochondrial enzymes of lymphocytic energy metabolism were studied in patients with infiltrative tuberculosis and healthy donors. The authors detected the significantly inhibited activity of succinate dehydrogenase and the redistribution of lymphocytic populations with the increased proportion of cells with the low and high activities of the enzyme. The more significant negative effect on the lymphocytes of patients with tuberculosis was observed when rifampicin was administered. In patients with pulmonary tuberculosis, the suppressed enzymatic activity of peripheral blood lymphocytes was shown to be more pronounced when the cells were incubated with rifampicin and isoniazid for 2 hours than for 1 hour.

[The superficial architectonics of peripheral blood lymphocytes in patients with pulmonary tuberculosis].

Peripheral blood lymphocytes were electron microscopically studied in patients with disseminated drug-sensitive and drug-resistant pulmonary tuberculosis before and during specific antituberculous chemotherapy. There was a tendency for an increase in the count of lymphocytes with membranous morphostructural changes during the therapy, which was more clearly defined in the drug-resistant type of the infectious process. It is suggested that antituberculous drugs have a damaging effect on the membrane of immunocompetent blood cells.

Clinical study of the efficiency of Poetam in the treatment of the anemic syndrome in pubertal uterine hemorrhages.

Clinical study of the efficiency of Poetam (affinity-purified antibodies to recombinant human erythropoietin) in the treatment of anemia in patients with pubertal uterine hemorrhages proved that combined therapy with Poetam and iron preparation normalized erythron parameters, structural and metabolic status of erythrocytes, and ferrokinetic parameters of the peripheral blood sooner than monotherapy with Poetam or sorbifer.

[Effect of ultradispersible powder of piezoelectric ceramics on the structure of a peripheral red blood cell population].

Ultradispersible powder (UDP) of piezoelectric ceramics, which contains the oxides of lead, bismuth, chromium, etc. was tested for its effects on the structure of a peripheral red blood cell population in the staff maintaining the pilot commercial plants to manufacture piezoceramic UDP and in rats (by using an experimental model of chronic UDP intoxication). A number of general regularities of a red blood cell response to piezoelectric ceramic UDP were identified: despite the absence of visible changes in the values of red blood, there were significant peripheral erythrocytic structural changes shown as the decreased levels of diskocytes, the larger number of degenerative, prehemolytic, and transient forms of cells.

[The surface architectonics, functional characteristics, and metabolism in peripheral blood lymphocytes in acute herpes virus infection]. / Poverkhnostnaia arkhitektonika, funktsional'nye osobennosti i metabolizm limfotsitov perifericheskoi krovi pri khronicheskoi gerpesvirusnoi infektsii.

Twenty-five patients (males--11, females--14; aged 18 to 45) with chronic herpes virus infection relapsing at the time of clinical tests were examined, including investigations of the surface architectonics, the ability to blast-transformation and the metabolic status of peripheral-blood lymphocytes. Chronic herpes virus infection was found to be concurrent with changes of the percentage ratio of lymphocytes with the surface micro-patterns of different types, with a more intense activity of the immunity T-chain and with a changed nature of the intracellular metabolism of lymphocyte cells.

[Effects of ultra-dispersed piezoceramics Pb powders on staff's health status]. / Vliianie ul'tradispersnykh poroshkov p'ezokeramiki na sostoianie zdorov'ia personala.

The ultradispersed piezoceramic powder containing lead oxides, zirconium, titanium, bismuth, cadmium, manganese, strontium, and chromium was tested for its effect on the health status of the staff serving pilot industrial units producing the powder. There was a significant increase in the incidence of digestive disease, a tendency for respiratory and osteomuscular diseases to increase. It is concluded that it is necessary to use individual means of the protection and prophylaxis of the observed diseases.

[Nucleic acid content in peripheral blood lymphocytes from patients with infectious mononucleosis]. / Soderzhanie nukleinovykh kislot v limfotsitakh perifericheskoi krovi pri infektsionnom mononukleoze.

An increase in RNA content in peripheral blood lymphocytes and increased number of macronuclear weakly basophilic cells (presumably due to increased content of B-lymphocytes which, in contrast to sharply basophilic T-lymphocytes, are characterized by light basophilic cytoplasm) are observed in patients with infectious mononucleosis. Macronuclear forms represented equally by hyper- and hypobasophilic elements predominate the structure of atypical mononuclears. Increased content of DNA in the peripheral blood lymphocytes is due to increased number of cells with high DNA content in the patient's blood and appearance of cells with 52-65 arb. units DNA (blast-transformed atypical mononuclears infected with the virus) which are normally absent.

Morphological characteristics of peripheral blood erythrocytes in rats subjected to chronic inhalation of ultradispersed piezoelectric ceramic powder.

In albino rats subjected to chronic inhalation of ultradispersed piezoceramic powder, scanning electron microscopy revealed changes in membrane surface of peripheral blood erythrocytes manifested as decreased number of diskocytes and increased number of transitional, prehemolytic, and degenerating forms. These parameters returned to normal 1 month after termination of inhalations.

[Mechanism of hematopoietic disorders in primary experimental hypothyroidism]. / K mekhanizmu narusheniia gemopoéza pri pervichnom éksperimental'nom gipotireoze.

In male BALB/c mice and male Wistar rats, primary hypothyrosis was induced by potassium perchlorate whose 1% solution was given to the experimental animals instead of portable water for 3 months. Blood features and medullary hemopoiesis were studied. The changes of the parameters under study were unidirectional in the two animal species, but they were more profound in the rats. The animals with primary hypothyrosis had lower counts of erythrocytes, reticulocytes, and hemoglobin. The bone marrow exhibited a reduction in the total count of myelokaryocytes, suppressed proliferative activity of immature cells and erythroid and myeloid populations. Examining the effects of T3 on cultured bone marrow cells of hypothyrosis rats revealed a sharp T3-induced increase in the count of erythroid cells and the level of their K-mitoses.

[Status of nucleoprotein synthesis in hematopoietic organs in mice with primary hypothyroidism]. / Sostoianie sinteza nukleoproteinov v organakh gemopoéza u myshei s pervichnym gipotireozom.

A rate of incorporation of 3H-thymidine, 3H-uridine and 14C-glycine into the nucleoproteins of bone marrow, spleen, thymus and ileocecal lymph nodes was studied in BALB/c mice with hypothyrosis developed after administration of 1% KClO4 within a month. Distinct hypoplasia of bone marrow and spleen was found in the experimental animals; the synthesis of nucleoproteins was most impaired in bone marrow. The dependence of hemopoietic tissues on thyroid hormones was decreased as follows: bone marrow > spleen > thymus > lymph node. After subcutaneous administration of triiodothyronine (T3) in a dose of 50 mg/kg daily within 3 days of synthesis of nucleoproteins was considerably stimulated and the amount of cells was increased in bone marrow and spleen. Less distinct stimulating effect of T3 was observed in the thymus and lymph nodes of these animals.