Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 25(6): 1713-1717, 2017 Dec.
Article in Chinese | MEDLINE | ID: mdl-29262903

ABSTRACT

OBJECTIVE: To investigate the effect of myeloma-derived exosomes on surface activating receptors of NK cells, and to explore the mechanism of the function defect of NK cells. METHODS: The exosomes from the supernatant of multiple myeloma cell lines RPMI8226 and U266 were extracted by ultracentrifugation, and the size of them was identified under electron microscope; the human primary NK cells were extracted, and were co-cultured with the myeloma-derived exosomes (40 µg/ml), then the expression levels of surface activating receptors NKp46, NKp30 and NKG2D of NK cells at 0,1,4 and 24 hours were detected by flow cytometry. RESULTS: The exosomes showed small vesicular, sized 30-100 nm under electron microscope. The expression of surface activating receptors of NK cells declined at different degree after co-cultured with myeloma-derived exosomes. CONCLUSION: Myeloma-derived exosomes can inhibit the expression of surface activating receptors of NK cells.


Subject(s)
Exosomes , Multiple Myeloma/pathology , Receptors, Cell Surface/metabolism , Receptors, Natural Killer Cell/physiology , Humans , Killer Cells, Natural , NK Cell Lectin-Like Receptor Subfamily K
SELECTION OF CITATIONS
SEARCH DETAIL
...