METTL3 promotes choriocarcinoma progression by activating the miR-935/GJA1 pathway in an m6A-dependent manner.

The emerging role of microRNA-935 (miR-935) in modulating cancer progression has been recognized. However, its role in regulating choriocarcinoma (CCA) development and progression remains unknown. The present work aims to reveal the effect of miR-935 on CCA cell tumor properties and the related mechanism. The RNA expression of methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit (METTL3), miR-935, and gap junction protein alpha 1 (GJA1) was detected by quantitative real-time polymerase chain reaction. Protein expression of GJA1, Ki67, and METTL3 was measured by western blotting and immunohistochemistry assays. CCK-8 and colony formation were used to analyze cell proliferation. Transwell assays were performed to assess cell migration and invasion. Angiogenesis was investigated by tube formation assay. Xenograft mouse model assay was used to determine miR-935-mediated effect on tumor formation in vivo. The luciferase reporter assay and RNA pull-down assay were used to verify the relationship between miR-935 and GJA1. MeRIP assay was used to analyze the m6A methylation of pri-miR-935. MiR-935 expression was significantly upregulated in CCA tissues and cells when compared with control groups. MiR-935 overexpression promoted CCA cell proliferation, migration, invasion, and tube formation and tumor tumorigenesis in vitro and in vivo, but miR-935 knockdown showed the opposite effects. In addition, miR-935 targeted GJA1 and mediated CCA cell tumor properties by negatively regulating GJA1 expression. METTL3 promoted miR-935 maturation by inducing m6A methylation of pri-miR-935, and its overexpression contributed to CCA cell tumor properties through the regulation of miR-935. METTL3 promoted choriocarcinoma progression by m6A-dependently activating the miR-935/GJA1 pathway.

Effects of initial temperature and moisture content on heat generation during degradation of municipal solid waste.

Heat generation from degradation of organic matter in municipal solid waste (MSW) often leads to increased landfill temperature. However, it is difficult to measure environmental heat loss in laboratory and field tests; therefore, little research has been conducted to evaluate heat generation during waste degradation under different initial temperatures and moisture contents. In this study, tests were conducted to investigate the effects of initial temperature and moisture content on heat generation during waste degradation. A simple formula for calculating heat generation was proposed. Within 200 h, the waste temperature decreased by about 70%, and lower initial moisture contents were associated with greater temperature decreases. The smallest temperature decrease of 47% and the greatest heat generation occurred when the initial temperature was 40 °C. The initial moisture content increased from 30% to 60% and the heat generation increased from 5% to 36%. The heat generation per unit mass of organic matter during the aerobic and anaerobic stages were 19.44-23.77 and 0.27-0.50 MJ·kg-1, respectively, indicating that the proposed formula for calculation of heat generated from waste degradation was reasonable. The results presented herein provide theoretical support for the prediction of heat generation and the recycling of heat resources in MSW landfill sites.

Engineering Properties of Novel Vertical Cutoff Wall Backfills Composed of Alkali-Activated Slag, Polymer-Amended Bentonite and Sand.

The workability, hydraulic conductivity, and mechanical properties are essential to contaminant containment performance of cementitious backfills in vertical cutoff walls at contaminated sites. This study aims to investigate the engineering properties of a novel vertical cutoff wall backfill composed of reactive magnesia (MgO)-activated ground granulated blast furnace slag (GGBS), sodium-activated calcium bentonite amended with polyacrylamide cellulose (PAC), and clean sand (referred to as MSBS-PAC). Backfills composed of MgO-activated GGBS, sodium-activated calcium bentonite, and clean sand (referred to as MSBS) were also tested for comparison purposes. A series of tests were conducted which included slump test, flexible-wall hydraulic conductivity test, and unconfined compression test. The pore size distributions of two types of backfills were investigated via the nuclear magnetic resonance (NMR) technique. The results showed the moisture content corresponding to the target slump height was higher for MSBS-PAC backfill than that for MSBS backfill. The MSBS-PAC backfill possessed lower pH, dry density, and higher void ratio at different standard curing times as compared to MSBS backfill. The unconfined compressive strength and strain at failure of the MSBS-PAC backfill were noticeable lower than those of the MSBS backfill. In contrast, the hydraulic conductivity of MSBS-PAC backfill was approximately one order of magnitude lower than that of the MSBS backfill, which was less than 10-9 m/s after 28-day and 90-day curing. Lower hydraulic conductivity of MSBS-PAC backfill was attributed to the improvement of pore structure and pore fluid environment by PAC amendment.

Strength Characteristics of a Smooth HDPE Geomembrane/Nonwoven Geotextile Interface Based on a Novel Ring Shear Apparatus.

This paper aims to investigate the interfacial strength characteristics, particularly the residual strength, of a high-density polyethylene smooth geomembrane (GMB-S)/nonwoven geotextile (NW GTX) interface using a novel ring shear apparatus under high normal stresses and two specimen conditions. A total of eight normal stresses (from 50 kPa to 2308 kPa) and two specimen conditions (dry and submerged at ambient temperature) are considered in this study. The reliability of using the novel ring shear apparatus to study the strength characteristics of the GMB-S/NW GTX interface was demonstrated by conducting a series of direct shear experiments with a maximum shear displacement of 40 mm and ring shear experiments with a shear displacement of 10 m. The peak strength, post-peak strength development, and residual strength determination method of the GMB-S/NW GTX interface are explained. Three exponential equations suitable for characterizing the relationship between the post-peak friction angle and the residual friction angle of the GMB-S/NW GTX interface are established. This relationship can be used with the relevant apparatus (i.e., an apparatus with deficiencies in executing large shear displacement) in determining the residual friction angle of the high-density polyethylene smooth geomembrane/nonwoven geotextile interface.

Investigating the Deformation Characteristics of Buried High-Density Polyethylene Pipes: Considering the Effect of Sequentially Applying Pressure and Elevating Temperature.

High-density polyethylene (HDPE) materials have many applications in the municipal solid waste (MSW) landfills. HDPE gravity drainage pipes are commonly utilized in MSW landfills because of the polymer's resistance to harsh chemical conditions. When landfill wastes are freshly filled, the weight acting on the leachate collection pipe increases. The temperature of the leachate collection pipe increases as a result of the heat produced by the decomposition of organic components after waste filling. In this paper, the effects of sequentially applying pressure and elevating temperature on the deformation characteristics (such as deformations and strains) of HDPE pipes are investigated. Measurements of pipe deformations and circumferential strains from model experiments in which 110 mm HDPE pipes were backfilled with sand and subjected to 300 kPa of maximum vertical pressure at temperatures of 20, 60 and 80 °C showed the following results: (1) a classification of pipe behavior relative to the surrounding soil stiffness is advantageous for HDPE pipe design; (2) when temperature increases to 60 °C and 80 °C, the strain distribution around the pipe changes from V-shaped to U-shaped, and the pipe deformation profile changes from elliptical to rectangular; (3) when temperature increases from 20 °C to 60 °C, the vertical and horizontal pipe deflections increase by a factor of 1.08~1.19; (4) when temperature increases from 60 °C to 80 °C, the vertical and horizontal pipe deflections increase by a factor of 1.15~1.31; and (5) the existing analytical method that considers two extreme interfaces can capture the deformations measured in the model test well. In addition, preliminary recommendations for the design of leachate collection pipes are provided based on the analysis of differences in pipe profile versus temperature.

Simulation of heat transfer in a landfill with layered new and old municipal solid waste.

Due to rapid degradation of the newly filled municipal solid waste (MSW), the local temperature of the waste layer increases greatly. The mechanical parameters related to waste degradation and the deformation of high-density polyethylene (HDPE) pipes in the waste body will be affected by the elevated temperature. To predict the temperature distribution in the anaerobic landfill, a one-dimensional heat transfer model is established in this study. This model considers the stratification of the saturated and unsaturated zones, and the layering of new and old waste. Furthermore, a single peak model for heat production is applied as the source term of heat production. The stratification of the unsaturated and saturated zones is considered by distinguishing the difference in heat conductivity and specific heat capacity. The layering of the new and old waste layers is considered by distinguishing the difference in the length of time that waste has been degraded to produce heat. Based on the numerical calculation method, the temperature distribution in a landfill with layered new and old MSW is well simulated. The position where the maximum temperature occurs and the variation in the temperature at the edge of new and old waste are elucidated. The sensitivity analysis shows that the influence of the density on the temperature distribution is more significant. Besides, the stratification of saturated-unsaturated waste should also be considered in landfills.

Identification of Circular RNA circ_0017068 as a Regulator of Proliferation and Apoptosis in Trophoblast Cells by miR-330-5p/XIAP Axis.

Preeclampsia (PE) is a major and serious complication of pregnancy. Circular RNAs (circRNAs) have been implicated in the initiation and progression of PE. In this paper, we explored the precise actions of circ_0017068 in trophoblast cell functional properties. Ribonuclease (RNase) R, and Actinomycin D treatments were used to characterize circ_0017068. The levels of circ_0017068, microRNA (miR)-330-5p and X-linked inhibitor of apoptosis protein (XIAP) were measured by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot analysis. Cell proliferation, cell cycle progression, and apoptosis were gauged by the Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), and flow cytometry assays, respectively. Direct relationship between miR-330-5p and circ_0017068 or XIAP was validated by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Our data showed that circ_0017068 was downregulated in PE placental samples. Enforced expression of circ_0017068 promoted HTR-8/SVneo cell proliferation, cycle progression, and suppressed apoptosis, while silencing of circ_0017068 exhibited opposite effects. Mechanistically, circ_0017068 targeted miR-330-5p, and circ_0017068 regulated proliferation, cycle progression, and apoptosis of HTR-8/SVneo cells through miR-330-5p. Moreover, XIAP was identified as a direct and functional target of miR-330-5p. Furthermore, circ_0017068 operated as a post-transcriptional regulator of XIAP expression through miR-330-5p. Our study identifies circ_0017068 as an important regulator of the proliferation and apoptosis of HTR-8/SVneo trophoblast cells at least in part by miR-330-5p-dependent regulation of XIAP, highlighting circ_0017068 as a potential therapeutic agent for PE treatment.

Effect of gas pressure on municipal solid waste landfill slope stability.

Slope failure in municipal solid waste (MSW) landfills is a common environmental disaster that poses serious ecological and health risks. Landfill slope stability (SS) is sensitive to leachate levels and gas pressure (GP) caused by the degradation of organic material, but the extent of these combined effects remains poorly understood. In this study, a simplified landfill GP calculation method is presented and a circular slide method that considers the combined effects of leachate and GP is established. The results show that the landfill GP is mainly affected by the gas production rate, gas conductivity of the solid waste (SW), and landfill depth. The safety factor of landfill SS is also significantly lower when GP is considered. The distribution of GP is affected by the depth of the failure circle and SW. Landfill slope instability can be explained by localized damage caused by GP breakthrough of the filled SW. This study probably provides important guidance for the design, operation, and management of MSW landfills.

Wedge method for landfill stability analysis considering temperature increase.

The increase in landfill temperature often results in shear strength reduction of both the solid waste and the liner, which leads to slope instability. However, very few landfill slope analysis methods can simultaneously consider the effect of temperature on the shear strength of the waste solid and the liner. In this study, based on the strength parameters of the liner and waste with temperature, a wedge method for translational failure analysis of landfills considering temperature increase was established. The results showed that rising temperatures caused by biochemical degradation at the bottom and middle of the landfill reduced the anti-slide force of back slope more than that of bottom slope. With the leachate level increasing, the effect of temperature rise on landfill stability became obvious. The feasibility of the proposed wedge method was verified by the engineering case study of Xiaping Landfill, Shenzhen, China. This study probably provides important guidance for the design, operation and management of municipal solid waste landfills.

Simulation of gas transport in a landfill with layered new and old municipal solid waste.

Average biodegradation rate of newly filled municipal solid waste (MSW) in landfills is relatively fast, and the landfill gas produced by the new MSW biodegradation can cause great variations in gas pressure. To predict the gas pressure distribution in the MSW layer, a one-dimensional gas transport model is established in this study. The following factors are considered in this model: (1) the variation of gas permeability with depth; (2) the anisotropy ratio of gas permeability; (3) the settlement caused by waste biodegradation. Furthermore, a single peak model for gas production is applied as the source term of gas production. The equation for settlement caused by waste biodegradation is presented, and the time of peak gas production rate is obtained by fitting the settlement of the newly filled layer. The stratification of the unsaturated and saturated regions is taken into account by distinguishing the difference in gas saturation. The layering of the new and old waste layers is considered by distinguishing the difference in the length of time that waste has been degraded to produce gas. Based on the method of numerical calculation, the gas pressure distribution in the landfill with layered new and old MSW is well simulated. The position where the maximum gas pressure occurs is found. The sensitivity analysis shows that the influence of the anisotropy ratio on gas pressure distribution is more significant.

Effect of elevated temperature on solid waste shear strength and landfill slope stability.

Slope instability occurs in landfills owing to increased internal temperatures. However, strength characteristic tests for solid waste (SW) and landfill slope stability (SS) calculations that consider temperature variations are scarce in the literature. In this study, we conducted triaxial tests on SW under a range of temperature conditions and proposed the circular slide method (CSM) for calculating SS in consideration of temperature effects. SW cohesion decreased linearly with increasing temperature, whereas the internal friction angle remained essentially unchanged. Our results showed that higher temperatures reduced the SW shear strength, changing the most dangerous sliding arc away from the slope toe. The landfill slope safety factor decreased by more than 20% with an increase of the maximum temperature from 20°C to 50°C. Reduction of the leachate level (LL) led to a decrease in the landfill high-temperature zone and the safety factor increased according to LL and temperature distribution. If cooling pipes are used to control the SW temperature, we recommend arranging the cooling pipes on the landfill liner. The proposed CSM can be used to analyse landfill SS.

A modified stability analysis method of landfills dependent on gas pressure.

Excessive gas pressure (GP) in landfills is a potential triggering mechanism for slope failure, which is rarely considered in analytical models. This paper presents a modified analytical model for landfill stability dependent on GP. A two-layered GP model is established to describe the GP above and below the leachate level. The distribution of GP is then used to calculate the factor of safety (FS) using a modified wedge stability analysis method. It is found that the lack of consideration of the GP in landfill stability analysis leads to serious overestimation of the FS. In addition, the GP gas pressure within the landfill accelerates the critical interface of a multilayer liner system shifting from one to another. A new estimation criterion for FS is proposed. The proposed criterion can directly estimate the stability of the landfill by the field-tested pore pressure. Finally, the proposed method is applied to estimate the slope failure of Xiaping landfill in Shenzhen, and the results verify the proposed method.

Simulation of gas-leachate pressure in various tested landfills using the differential quadrature method.

The degradation of solid waste in landfills results in the coupled migration of gas and leachate through the pore spaces in waste material. The existing analytical methods cannot be used to obtain a solution for the gas-leachate coupled migration problem. This study used the differential quadrature method to solve the gas and leachate phase continuity equations considering the effect of the gas-leachate coupling. The calculation results were verified based on the calculated data of previous studies. The results of the field gas collection tests and the laboratory degradation tests were fitted using the peak gas generation equation. The peak values of gas generation were found between 0.94 and 20.29 years in the field tests, and between 0.09 and 0.19 years in the laboratory tests. The gas pressure calculated by parameters fitting of the field tests and the laboratory tests were less than 1 kPa and greater than 8 kPa, respectively. Considering the gas-leachate coupling effect, the pore gas pressure in the simulated landfill increased by approximately 20%, and the peak pore gas pressure occurred slightly earlier than that without consideration of the coupling effect.

A unit-cell model for thermal regulation of degradation of organics in solid waste.

It is a well appreciated fact that temperature is one of the key factors influencing the degradation of organics. Heat exchangers are a viable option that can be used to adjust the temperature in solid waste to an extent most suitable for waste degradation. This paper focuses on an experimental and theoretical investigation of the feasibility of using a water-circulating heat exchanger for thermal regulation of waste degradation. A cylindrical bioreactor with a central pipe connected to a water circulation system is devised and instrumented. The changes in temperature and gas production were monitored during the degradation of the organic component of the waste. Test results with and without thermal regulation are analyzed and compared. In addition, an analytical model is proposed to simulate the symmetrical heat transport behavior subjected to heat exchange. Heat generation due to the degradation of organics is taken into account. There was a good correlation between the analytical model prediction and the experimental data obtained from the laboratory test and field monitoring.

A new method for determination of heavy metal adsorption parameters in compacted clay by batch tests.

Evaluation of adsorption properties of pollutants on artificial or natural clay strata is normally considered in investigations of soil and groundwater pollution. Batch adsorption tests can be used to obtain the adsorption parameters of clay particles; however, the results from these tests are usually very different from the adsorption of actual clay strata. If the adsorption parameters obtained by batch tests are used to directly evaluate the properties of adsorption of pollutants onto compacted clay, the predicted groundwater and soil pollution will be unsafe. Although the column diffusion tests are closer to the actual situation, they may require much more time, and diffusion and adsorption occur simultaneously in tests, making it difficult to accurately determine the adsorption parameters. To solve this problem, batch adsorption and column diffusion tests were conducted using three kinds of clay materials to investigate the mechanism of the differences in adsorption properties of heavy metal on clay particles and in compacted clay. The amount of adsorption per unit particle surface area of clay particles was found to be equal to that per unit pore surface area of compacted clay. A new simplified method was proposed to determine the adsorption parameters in compacted clay. It is easy to use and provide a reference for prediction and evaluation of soil and groundwater pollution.

Effect of the leachate head on the key pollutant indicator in a municipal solid waste landfill barrier system.

In previous studies of municipal solid waste (MSW) landfill barrier systems, chemical oxygen demand has been found to be the key pollutant indicator when estimating the breakthrough time for. However, the leachate head in a municipal solid waste landfill can be very high (>10 m in some Chinese landfills). The key pollutant indicator could be different at different leachate heads. The leachate head will continuously change during the use of a landfill. Different pollutants have different transport characteristics in the leachate, so it is necessary to determine whether changes in the leachate head change the key pollutant indicator for identifying breakthrough in a landfill barrier system. In this study, numerical models were used to investigate transport of common leachate pollutants through four typical landfill barrier systems with different leachate heads. Chemical oxygen demand reached the breakthrough threshold before the other pollutants, irrespective of (1) the leachate head and (2) changes in the leachate head. It was therefore clear that the leachate head did not affect the selection of the key pollutant indicator for identifying breakthrough in a landfill barrier system.

Temperature monitoring during a water-injection test using a vertical well in a newly filled MSW layer of a landfill.

High temperature may adversely affect municipal solid waste (MSW) biodegradation and lead to an increase in the deformation of high-density polyethylene (HDPE) pipes used for the collection of leachate and landfill gas in landfills. The test in this study was to change the waste temperature around the vertical injection well by water injection using a vertical well. The test was conducted intermittently with two different flowrates in a newly filled MSW layer of a landfill. The temperature, gas pressure and leachate level in the test area were simultaneously monitored during this study. The results showed that the waste temperature around the vertical injection well was effectively changed by water injection, which did not result in a significant rise in the leachate level. During water injection, the waste temperature influence distance in the horizontal direction increased with depth from the leachate level to the bottom of the injection well. The bottom temperature of the injection well decreased to near the water-injection temperature. The range of influence of the waste temperature caused by intermittent water injections slightly increased in this test. After water injection was stopped, the waste temperature near the vertical injection well increased quickly initially, and then the increments became more gradual with time. When the leachate level recovered stably, there was still a temperature gradient around the injection well within the range of influence. The temperature and gas pressure in the waste above the leachate level and far away from the injection well were slightly influenced by water injection.

Laboratory test investigations on soil water characteristic curve and air permeability of municipal solid waste.

The air permeability coefficient has a high correlation with the water content of municipal solid waste. In this study, continuous drying methodology using a tension meter was employed to construct the soil water characteristic curve of municipal solid waste (M-SWCC). The municipal solid waste air permeability test was conducted by a newly designed apparatus. The measured M-SWCC was well reproduced by the van Genuchten (V-G) model and was used to predict the parameters of typical points in M-SWCC, including saturated water content, field capacity, residual water content and water content at the inflection point. It was found that the M-SWCC was significantly influenced by void ratio. The final evaporation and test period of M-SWCC increase with the increase in void ratio of municipal solid waste. The evolution of air permeability coefficient with water content of municipal solid waste depicted three distinct characteristic stages. It was observed that the water contents that corresponded to the two cut-off points of the three stages were residual water content and water content at the inflection point, respectively. The air permeability coefficient of municipal solid waste decreased with the increase of the water content from zero to the residual water content. The air permeability coefficient was almost invariable when the water content increased from residual water content to the water content at the inflection point. When the water content of municipal solid waste exceeded the water content at the inflection point, the air permeability coefficient sharply decreased with the increase of water content.

Clinical and biomechanical researches of polyetheretherketone (PEEK) rods for semi-rigid lumbar fusion: a systematic review.

Lumbar spinal fusion using rigid rods is a common surgical technique. However, adjacent segment disease and other adverse effects can occur. Dynamic stabilization devices preserve physiologic motion and reduce painful stress but have a high rate of construct failure and reoperation. Polyetheretherketone (PEEK) rods for semi-rigid fusions have a similar stiffness and adequate stabilization power compared with titanium rods, but with improved load sharing and reduced mechanical failure. The purpose of this paper is to review and evaluate the clinical and biomechanical performance of PEEK rods. A systematic review of clinical and biomechanical studies was conducted. A literature search using the PubMed, EMBASE, and Cochrane Library databases identified studies that met the eligibility criteria. Eight clinical studies and 15 biomechanical studies were included in this systematic review. The visual analog scale and the Oswestry disability index improved significantly in most studies, with satisfactory fusion rates. The occurrence of adjacent segment disease was low. In biomechanical studies, PEEK rods demonstrated a superior load-sharing distribution, a larger adjacent segment range of motion, and reduced stress at the rod-screw/screw-bone interfaces compared with titanium rods. The PEEK rod construct was simple to assemble and had a reliable in vivo performance compared with dynamic devices. The quality of clinical studies was low with confounding results, although results from mechanical studies were encouraging. There is no evidence strong enough to confirm better outcomes with PEEK rods than titanium rods. More studies with better protocols, a larger sample size, and a longer follow-up time are needed.

Prototype heat exchange and monitoring system at a municipal solid waste landfill in China.

A prototype heat exchange and monitoring system was installed and operated in a newly filled municipal solid waste (MSW) landfill, located in Wuxi City, China. In this study, a test was conducted using the system to investigate the influence of heat exchange on waste temperature over three heat exchange stages. During the period of sharp increase of waste temperature, the first stage test was performed, whereas during the period of gradual increase of waste temperature, the second and third stages were performed. The results showed that (1) the waste temperature increased during the first two months after the commencement of temperature observations. This increase in temperature partially counteracted the effect of the heat exchange system on waste temperature during the first stage test. (2) During the subsequent 360 days, the waste temperature increased gradually. The influence of the heat exchange system was relatively more effective during the second and third stages than during the first stage. (3) During the test, the waste temperatures showed similar changes throughout the saturated portion of the waste, which was below the leachate level. (4) At ten days after the third heat exchange stage, the waste temperature increased gradually to the previous elevated temperature. The results of the test demonstrate that waste temperatures are mainly affected by thermal conduction in the waste mass and thermal convection in the leachate. Moreover, preliminary suggestions are provided for the installation of heat exchange pipes in landfills.