ABSTRACT
Objective: To investigate the changes of metabolites of teenage football players after exercise-induced fatigue. Methods: Twelve male teenage football players (14ï½16 yrs) were selected as experimental subjects in this study. And an exercise model including aerobic and anaerobic exercise as one group exercise was established by using power bicycle: completion 6 min 150 W load, 60ï½65 r/min of riding exercise and 30 s of riding exercise which load was the maximum speed set by the tester's weight. The rest took 1 min in the middle of one group exercise, and repeat 3 times of one group exercise, then rest for 3 min after one group exercise. The maximum oxygen uptake (VO2 max) and average anaerobic power were measured after each group exercise. Their urine samples were collected before and after the whole exercise model, and gas chromatograph-mass spectrometer (GC-MS) was used to detect the differential metabolites. Results: The teenage football players had a significant decrease in anaerobic capacity after fatigue. Compared with pre-exercise, a total of 25 differential metabolites were screened out, of which 3 metabolites were significantly higher and 22 metabolites were markedly lower. The related metabolic pathways of above differential metabolites were classified as glycine-serine-threonine metabolism, tricarboxylic acid cycle, tyrosine metabolism, nitrogen metabolism and glycerophospholipid metabolism, respectively. Conclusion: After exercise-induced fatigue occurs in teenage football players, the body's metabolites: sarcosine, L-allothreonine, creatine, serine, succinic acid, citric acid, 4-hydroxyphenylacetic acid, hydroxylamine, and ethanolamine produce significant changes. The above-mentioned differential metabolites can be used as indicators for teenage football players' exercise-induced fatigue evaluation.
