ABSTRACT
Triallyl isocyanurate (TAIC) was modified by hydrogen silicone oil (SO) via hydrosilylation reaction, generating the original TAIC-SO (TS) intermediate. After the cross-linking polymerization of TS (PTS), the shape-stabilized phase change materials (PCMs) consisting of n-octadecane and silicone-modified supporting matrix were first synthesized by an in situ reaction. Remarkably, the novel three-dimensional PTS network effectively prevents the leakage of n-octadecane during its phase transition, solving the prominent problem of solid-liquid PCMs in practical applications. Moreover, n-octadecane is uniformly dispersed in the continuous and high-strength cross-linked network, contributing to excellent thermal reliability and structural stability of PTS/n-octadecane (TSO) composites. Differential scanning calorimetry analysis of the optimal TSO composite indicates that melting and freezing temperatures are 29.05 and 22.89 °C, and latent heats of melting and freezing are 130.35 and 129.81 J/g, respectively. After comprehensive characterizations, the shape-stabilized TSO composites turn out to be promising in thermal energy storage applications. Meanwhile, the strategy is practical and economical due to its advantages of easy operation, mild conditions, short reaction time, and low energy consumption.
ABSTRACT
OBJECTIVE@#To explore the mechanisms underlying the proliferative inhibition of Chinese herbal medicine Kang-Ai injection (KAI) in gastric cancer cells.@*METHODS@#Gastric cancer cell lines MGC803 and BGC823 were treated by 0, 0.3%, 1%, 3% and 10% KAI for 24, 48 and 72 h, respectively. The cell proliferation was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. The apoptosis and cell cycle were evaluated by flow cytometry. Interleukin (IL)-6 mRNA and protein expression levels were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immune sorbent assay (ELISA), respectively. The protein expression levels of cyclin A, cyclin E, cyclin B1, cyclin D1, p21, retinoblastoma (RB), protein kinase B (AKT), extracellular regulated protein kinases (ERK), signal transducer and activator of transcription (STAT) 1 and STAT3 were detected by Western blot.@*RESULTS@#KAI inhibited the proliferation of MGC803 and BGC823 gastric cancer cells in dose- and time-dependent manner. After treated with KAI for 48 h, the proportion of G1 phase was increased, expression level of cyclin D1 and phosphorylation-RB were down-regulated, whereas the expression of p21 was up-regulated (all P<0.01). Furthermore, 48-h treatment with KAI decreased the phosphorylation level of STAT3, inhibited the mRNA and protein expressions of IL-6 (all P<0.01). IL-6 at dose of 10 ng/mL significantly attenuated the proliferative effect of both 3% and 10% KAI, and recovered KAI-inhibited STAT3 phosphorylation and cyclin D1 expression level (all P<0.01).@*CONCLUSION@#KAI exerted an anti-proliferative function by inhibiting IL-6/STAT3 signaling pathway followed by the induction of G1 phase arrest in gastric cancer cells.
