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Near-infrared fluorescent proteins.

Shcherbo, Dmitry; Shemiakina, Irina I; Ryabova, Anastasiya V; Luker, Kathryn E; Schmidt, Bradley T; Souslova, Ekaterina A; Gorodnicheva, Tatiana V; Strukova, Lydia; Shidlovskiy, Konstantin M; Britanova, Olga V; Zaraisky, Andrey G; Lukyanov, Konstantin A; Loschenov, Victor B; Luker, Gary D; Chudakov, Dmitriy M.

Nat Methods ; 7(10): 827-9, 2010 Oct.

Article in English | MEDLINE | ID: mdl-20818379

ABSTRACT

Fluorescent proteins with emission wavelengths in the near-infrared and infrared range are in high demand for whole-body imaging techniques. Here we report near-infrared dimeric fluorescent proteins eqFP650 and eqFP670. To our knowledge, eqFP650 is the brightest fluorescent protein with emission maximum above 635 nm, and eqFP670 displays the most red-shifted emission maximum and high photostability.

Subject(s)

Biotechnology/methods , Luminescent Proteins , Whole Body Imaging/methods , Amino Acid Sequence , Animals , Biotechnology/instrumentation , Embryo, Nonmammalian/cytology , Embryo, Nonmammalian/drug effects , Embryo, Nonmammalian/metabolism , Escherichia coli/genetics , Escherichia coli/growth & development , HeLa Cells , Humans , Infrared Rays , Luminescent Proteins/genetics , Luminescent Proteins/toxicity , Mice , Molecular Sequence Data , Protein Multimerization , Protein Stability , Sequence Alignment , Transfection , Xenopus laevis/genetics , Xenopus laevis/metabolism , Zebrafish/genetics , Zebrafish/metabolism

ABSTRACT

Subject(s)

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